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Gene information
General information
TC006211 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 4790150..4794114
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006211
    iBeetle screen iB_04088
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006211 RA
      MKPLNIEII LKNQSHISK TFITSITLH YLETIKVGS 
      NTVITQFDQ DHHPILHSE VKAIIVEEI PGLVGFINF 
      SQYEVHWHV YTLDNDGPQ SQIEEGEDE GQLNFATHL 
      MLPSAHIYN LWENLHYDN NIKQNLLKY AQTMMEFSD 
      RGVDTNIVN CNRVILLHG PPGTGKTSL CKALAHKLA 
      IRMQERYNS GVLIEINSH SLFSKWFSE SGKLVTKMF 
      TKIIEIVEN SNLLVCVLI DEIESLAHA RNQCISGNE 
      PSDSIRVVN AVLTQIDRI KRYSNVLIL TTSNITESI 
      DLAFIDRAD IKQYLGLPT PTAIYKVYH SCFEELTKA 
      EIIKKGEQG NEETQKLWQ ICEKSKGLS GRSLRKIPF 
      IAHALYLEG TCIDLGKFL DAMLSAIER EKKERQYFE 
      KDLKS
    CDS sequence
    • >TC006211 RA
      atgaaaccc ctcaatatt gaaattatt ttaaagaac 
      caaagccac atttcgaaa acgttcata acttcaata 
      accctgcat tatttggaa actatcaaa gtaggaagt 
      aatacagta attacgcag tttgaccaa gaccatcac 
      cccatactt cactcagaa gttaaagcg atcattgtt 
      gaggaaatc ccaggcctt gttggtttc atcaacttt 
      agccaatat gaagtacat tggcacgtt tacactctt 
      gacaatgac ggcccccag agccaaatc gaggagggc 
      gaggacgag ggacagcta aattttgcc actcatttg 
      atgctacct tcagctcat atttacaac ttatgggag 
      aacctccac tacgataac aacatcaaa caaaacttg 
      cttaaatac gcccaaaca atgatggaa ttctcagac 
      cgtggtgtt gacacaaac atcgtcaat tgcaaccgt 
      gttatactc ctccatggc cctccagga accggtaaa 
      acatcccta tgtaaagcc ctagcccat aaattagcg 
      attcgcatg caagaacgc tacaattct ggcgtttta 
      atcgaaatc aacagtcac agtttattc tccaagtgg 
      ttttccgag agtggtaaa ttggtgacg aaaatgttt 
      acaaaaatc atcgaaatc gtagaaaat tcaaatttg 
      ttagtttgt gttttgatc gacgaaatc gagtcgctt 
      gcgcatgcg cggaaccaa tgcatttca ggtaacgaa 
      ccgtcggat tcgatccgc gtcgtcaac gccgttttg 
      acccaaatc gatcgaatc aagcgatac tcgaatgtc 
      ttaatcctg acaacttcg aatattacc gaatcaatc 
      gacttagca ttcattgat agagccgac attaaacaa 
      tacttaggg ctaccaacc ccaaccgct atttacaag 
      gtgtaccac tcgtgcttc gaggaatta actaaagcg 
      gaaattatt aaaaagggc gagcaggga aatgaagaa 
      acgcagaaa ttgtggcaa atctgcgag aaaagtaaa 
      ggtttgagc ggccgatct ttaaggaaa attcccttc 
      atagcgcat gcgctttac ctggagggg acttgcatc 
      gatttgggg aaatttctc gacgccatg ttgagtgcc 
      atcgagcgg gaaaaaaag gagcgccaa tatttcgaa 
      aaagatctc aaatcgtaa
    mRNA sequence
    • >TC006211 RA
      ctcacttta aattcaaat ttgtcggtt atttgtttt 
      gaattttag aatgaaacc cctcaatat tgaaattat 
      tttaaagaa ccaaagcca catttcgaa aacgttcat 
      aacttcaat aaccctgca ttatttgga aactatcaa 
      agtaggaag taatacagt aattacgca gtttgacca 
      agaccatca ccccatact tcactcaga agttaaagc 
      gatcattgt tgaggaaat cccaggcct tgttggttt 
      catcaactt tagccaata tgaagtaca ttggcacgt 
      ttacactct tgacaatga cggccccca gagccaaat 
      cgaggaggg cgaggacga gggacagct aaattttgc 
      cactcattt gatgctacc ttcagctca tatttacaa 
      cttatggga gaacctcca ctacgataa caacatcaa 
      acaaaactt gcttaaata cgcccaaac aatgatgga 
      attctcaga ccgtggtgt tgacacaaa catcgtcaa 
      ttgcaaccg tgttatact cctccatgg ccctccagg 
      aaccggtaa aacatccct atgtaaagc cctagccca 
      taaattagc gattcgcat gcaagaacg ctacaattc 
      tggcgtttt aatcgaaat caacagtca cagtttatt 
      ctccaagtg gttttccga gagtggtaa attggtgac 
      gaaaatgtt tacaaaaat catcgaaat cgtagaaaa 
      ttcaaattt gttagtttg tgttttgat cgacgaaat 
      cgagtcgct tgcgcatgc gcggaacca atgcatttc 
      aggtaacga accgtcgga ttcgatccg cgtcgtcaa 
      cgccgtttt gacccaaat cgatcgaat caagcgata 
      ctcgaatgt cttaatcct gacaacttc gaatattac 
      cgaatcaat cgacttagc attcattga tagagccga 
      cattaaaca atacttagg gctaccaac cccaaccgc 
      tatttacaa ggtgtacca ctcgtgctt cgaggaatt 
      aactaaagc ggaaattat taaaaaggg cgagcaggg 
      aaatgaaga aacgcagaa attgtggca aatctgcga 
      gaaaagtaa aggtttgag cggccgatc tttaaggaa 
      aattccctt catagcgca tgcgcttta cctggaggg 
      gacttgcat cgatttggg gaaatttct cgacgccat 
      gttgagtgc catcgagcg ggaaaaaaa ggagcgcca 
      atatttcga aaaagatct caaatcgta atatttttt 
      attaacacg ttcctcgcc gctgtgaaa tt