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Gene information
General information
TC006206 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5040349..5042511
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006206
    iBeetle screen iB_04087

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006206 RA
      MRLIRLLNK LPREVRQEL HSHDIQTKQ SFIDYYFSL 
      FEQHVRDFD EYEKSTLVK FSEGVVSNN LNSVRFLME 
      AIYNNVVRD DFLTVFERQ FEPKTTLFC NETFSQQEA 
      LHQLLNEIL VTNFKSYVL EQSAYLIKK HNNQGNYTK 
      EVELSYKEF LSRSYNITK SFKKSMSEA SREVWRCDP 
      RKHIRNETF VEITNFLHG FILNKANLD SRGQCRDEC 
      SEYKMVLKP KNCEKNYWC KKQTTCPKI IDCQYVDSS 
      MTVCSNIMA KHNRRYDYV QFADGKNFG AKKSCNGDS 
      DYLETYKFW LIWSCSYCF CLCDEGPKY FSDRYINLR 
      LAMSNISDN KVVTGLRFV KHNQIIHLQ VQEGKLLEH 
      GTIDPDSVQ WVPPENYKR TDRKIHEGQ DYQVITWEK 
      RSIELTEIM ARNGSVVTG VRFKKIGSR LELEIMITP 
      FNFTTGKLR NYSDQSSIF VEAPNIHLR HRFGKKITL 
      SSPDVPTRS TAPSTHYTT GKYIEFTTT DVYKDAAQT 
      TVPFFDSQQ VYNKNPVPL SGVGLFHRS RRGSGGFIA 
      PKIFTYDFS QHL
    CDS sequence
    • >TC006206 RA
      atgcgactt attagatta ttaaataaa ctaccacga 
      gaagtccga caagaattg cattcgcac gacatccag 
      accaagcaa agtttcatc gactattac ttctcactt 
      tttgagcaa cacgttaga gactttgac gaatatgaa 
      aaatcgact ttggttaaa ttcagtgaa ggtgtcgtt 
      tcaaataat ttaaattca gttcgtttc ttaatggaa 
      gcaatttac aacaatgtg gtcagagac gattttttg 
      acagtgttc gagagacaa ttcgagccc aaaacgact 
      cttttctgc aacgaaacc ttttcacaa caagaggcg 
      ctccatcaa ctgttaaac gaaattcta gtcacaaat 
      ttcaagagt tatgttctt gaacagtcg gcttacctt 
      atcaaaaaa cacaacaat caaggtaac tacaccaaa 
      gaggttgaa cttagctac aaagaattt ttgtcaagg 
      tcttacaac atcaccaag tcttttaaa aaatccatg 
      agcgaggcg tcacgagaa gtgtggaga tgtgaccct 
      cggaaacac atcagaaac gaaactttt gtcgaaatt 
      acgaatttt cttcacggt tttatcctc aataaagcc 
      aatttagat tctcgtggc caatgtcgc gacgagtgc 
      agtgagtac aaaatggtc ttaaaacca aaaaactgc 
      gagaaaaat tattggtgt aaaaaacag accacttgt 
      ccgaaaatt atcgactgc caatatgtt gattcgtcc 
      atgactgtg tgttccaac attatggca aaacataac 
      cgaaggtac gattacgta caatttgct gacggaaaa 
      aattttggg gcaaaaaaa tcatgcaat ggtgactca 
      gactatttg gaaacgtac aaattttgg ttgatttgg 
      agttgttcc tattgcttc tgcttgtgc gacgaaggg 
      ccaaaatat ttttcagac agatatata aatttacgg 
      ctcgccatg tcaaacata agcgataac aaagttgtc 
      accggcttg agattcgtg aaacacaac caaatcatt 
      cacttgcaa gtccaagaa ggtaaactc ctcgaacat 
      ggaacaatt gatccggat tcagtccaa tgggtcccg 
      ccagaaaat tacaaaaga actgacaga aaaatccac 
      gaaggccaa gactaccaa gtcatcact tgggaaaaa 
      cgaagcatc gagttaacc gaaattatg gcaagaaac 
      ggttcggtt gtaaccggt gtcagattt aagaaaatc 
      ggttcgcga cttgagctc gaaatcatg ataacaccg 
      tttaacttc accacagga aaactgaga aattattcc 
      gatcagtca agtattttc gtagaagct ccaaatatc 
      catctgaga cacagattt gggaaaaaa atcacttta 
      tcatccccg gatgttccc actcgatca accgcacct 
      tccactcat tacacaaca ggaaaatac attgaattt 
      acaaccaca gatgtgtat aaggatgca gctcaaacc 
      acggtacca tttttcgac tctcagcaa gtctacaac 
      aaaaatccg gtgcccctt tcaggggtc ggtctcttt 
      cacagaagt cggagagga tcagggggg tttatagcc 
      ccgaaaata ttcacttac gatttcagc caacatttg 
      tag
    mRNA sequence
    • >TC006206 RA
      tacgacacg aaaaagagg gaggaaaaa ttattcatg 
      gcattagat cgcatcaat cttagaata aaaataatg 
      gaaggccga cttcnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn nnnnnnnnn nnnnnnnnn 
      nnnnnnnnn nnnnnnnnn ncaccgtgg tatgcgact 
      tattagatt attaaataa actaccacg agaagtccg 
      acaagaatt gcattcgca cgacatcca gaccaagca 
      aagtttcat cgactatta cttctcact ttttgagca 
      acacgttag agactttga cgaatatga aaaatcgac 
      tttggttaa attcagtga aggtgtcgt ttcaaataa 
      tttaaattc agttcgttt cttaatgga agcaattta 
      caacaatgt ggtcagaga cgatttttt gacagtgtt 
      cgagagaca attcgagcc caaaacgac tcttttctg 
      caacgaaac cttttcaca acaagaggc gctccatca 
      actgttaaa cgaaattct agtcacaaa tttcaagag 
      ttatgttct tgaacagtc ggcttacct tatcaaaaa 
      acacaacaa tcaaggtaa ctacaccaa agaggttga 
      acttagcta caaagaatt tttgtcaag gtcttacaa 
      catcaccaa gtcttttaa aaaatccat gagcgaggc 
      gtcacgaga agtgtggag atgtgaccc tcggaaaca 
      catcagaaa cgaaacttt tgtcgaaat tacgaattt 
      tcttcacgg ttttatcct caataaagc caatttaga 
      ttctcgtgg ccaatgtcg cgacgagtg cagtgagta 
      caaaatggt cttaaaacc aaaaaactg cgagaaaaa 
      ttattggtg taaaaaaca gaccacttg tccgaaaat 
      tatcgactg ccaatatgt tgattcgtc catgactgt 
      gtgttccaa cattatggc aaaacataa ccgaaggta 
      cgattacgt acaatttgc tgacggaaa aaattttgg 
      ggcaaaaaa atcatgcaa tggtgactc agactattt 
      ggaaacgta caaattttg gttgatttg gagttgttc 
      ctattgctt ctgcttgtg cgacgaagg gccaaaata 
      tttttcaga cagatatat aaatttacg gctcgccat 
      gtcaaacat aagcgataa caaagttgt caccggctt 
      gagattcgt gaaacacaa ccaaatcat tcacttgca 
      agtccaaga aggtaaact cctcgaaca tggaacaat 
      tgatccgga ttcagtcca atgggtccc gccagaaaa 
      ttacaaaag aactgacag aaaaatcca cgaaggcca 
      agactacca agtcatcac ttgggaaaa acgaagcat 
      cgagttaac cgaaattat ggcaagaaa cggttcggt 
      tgtaaccgg tgtcagatt taagaaaat cggttcgcg 
      acttgagct cgaaatcat gataacacc gtttaactt 
      caccacagg aaaactgag aaattattc cgatcagtc 
      aagtatttt cgtagaagc tccaaatat ccatctgag 
      acacagatt tgggaaaaa aatcacttt atcatcccc 
      ggatgttcc cactcgatc aaccgcacc ttccactca 
      ttacacaac aggaaaata cattgaatt tacaaccac 
      agatgtgta taaggatgc agctcaaac cacggtacc 
      atttttcga ctctcagca agtctacaa caaaaatcc 
      ggtgcccct ttcaggggt cggtctctt tcacagaag 
      tcggagagg atcaggggg gtttatagc cccgaaaat 
      attcactta cgatttcag ccaacattt gtagttgtt 
      gtaattaaa tttttgcta aaataaagt gtgtgtttt 
      taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY