Status
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Gene information
General information
TC006202 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4939304..4943327
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006202
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04086

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006202 RA
        MSQVKQQES KKGAFLDRD DFASKLVIG PVDLCIESL 
        KLLQNCVAI LSMMYNMPK CPSFNVTHR MNIDTDPQV 
        VDPIIQNIE ELHDLELCE SSGITLAKM DRQSNVLKI 
        WYILLEGLS SASIMSAHK NQPFVVETL FKLLRDLID 
        NPGVNFGLY SINHLLLPM VQNWMRQNS KIQKPGEIW 
        QNFKHCCGL ASELVVDYL HHLQGFGPF VENPAATLA 
        LKQLLLILI ECITQPSEN IARLGTSCI RHVILSAGK 
        ILTGGQWEI LVVALHRAC TISLNPLHQ LTLAFKENS 
        DSFYGDLAT VKVAARKDS TVSENERLY ELAQQVFLM 
        QCQRNCLKC DKLCECDAS MTIDDRSYV FLLYPLDMS 
        SILNPDLYT VRVPFRNLV VGILAHQML VQTISSALL 
        QNLNHITPI LNILQINSC SLRGILTHV NAKHVDILL 
        KCLDVSNAR AKQFDVRPG LKFLTQKVG NLSKAANLY 
        TQANTSEVV QMIVLIELC LDGIEKYSI KPKDLKELL 
        GKDGKVRCS SDLDYVEQF LRRLQNKWE FLCESYINL 
        TINIPVQDS EDELKSSSD NLDQIADKP RPFKLADFK 
        QEEDTLSSS TDSEGYLEK EILPNVKTE EEAEKPKLR 
        QSCSLDETI LKKTKEVSS TDDETNDMA AKYTKQIHD 
        KYKVELNSI KYDTNTLLQ MRKSTISSD NITSCDSFE 
        KKTPSPRIN PFMVANVPP PQPIPPEIQ QQRAMSIFK 
        DSEAYKTTR IETMEACLE LLSSLSSEK LSPLATVLK 
        QGAVLLIGA QDEKIKNAA ENLLNRMNI SYIDHDNF
      CDS sequence
      • >TC006202 RA
        atgtcgcaa gttaaacag caagagagt aagaaaggg 
        gcgtttttg gatagagat gattttgct tcaaaactg 
        gtcatcgga ccggttgat ctatgcatc gaaagcctc 
        aaactcctc caaaattgc gtcgccatc ttatcaatg 
        atgtacaac atgccgaaa tgtccctcc tttaacgtc 
        acacatcgc atgaatatt gacaccgat ccgcaagtt 
        gttgatcca atcatccag aatattgaa gagttacac 
        gatttggaa ttgtgcgaa tcaagtggt attacttta 
        gccaaaatg gatcggcaa agtaacgtt ttaaaaatt 
        tggtacatc ctccttgaa ggactctcc tcagcttct 
        atcatgtca gctcataaa aaccaacca tttgttgtt 
        gaaaccctc ttcaaactt ttgagggat ttgatcgat 
        aacccgggc gttaacttt gggttatat tccataaat 
        cacttgcta ctcccgatg gttcaaaac tggatgcgc 
        cagaacagc aagatccaa aaaccgggc gaaatctgg 
        caaaacttc aagcactgt tgtggctta gctagtgag 
        cttgtggtc gattatttg caccatttg caaggtttc 
        ggccctttt gttgaaaac ccagccgct actttggcc 
        ctcaaacaa ctcctcttg atcctaatc gagtgtata 
        acacaacct agcgaaaac atagcccgg ttagggact 
        tcctgcatc cgtcacgtt attttaagt gctggtaag 
        attttgact ggagggcaa tgggaaatt ctggtcgtg 
        gccctgcac agggcttgc accatctcg cttaaccct 
        ctccaccaa ctaaccctc gcatttaaa gagaactcg 
        gatagtttt tacggcgac ttggcgaca gttaaagtc 
        gcagcgcgt aaagacagc acagtgtcc gaaaacgag 
        aggttgtac gagcttgcc cagcaagtt ttcctcatg 
        caatgccaa cgcaactgt ctcaagtgc gacaagttg 
        tgcgagtgt gatgccagt atgacgatt gatgatcgc 
        agttatgtt tttctcctc tatccgctt gacatgtct 
        tcgatttta aacccagat ttgtataca gtgagggtt 
        ccatttcgc aatttggtg gttgggatt ttggcgcat 
        caaatgctc gttcagacg atttccagc gcgcttttg 
        caaaatttg aaccatatc acgccgatt ttgaacatt 
        ttgcaaatc aattcgtgt agtttgagg gggattttg 
        acgcatgtt aacgccaaa catgtcgat attctgttg 
        aagtgtttg gatgtgagt aatgccaga gcgaagcag 
        tttgatgtc aggcccggc ttgaagttt ttgactcag 
        aaggttggg aatttgagc aaggcggcg aatttgtac 
        acgcaggca aacacttcg gaggtggtc caaatgatt 
        gttttaatc gagttgtgt cttgatggc attgaaaaa 
        tattcgatt aaacccaaa gacttgaaa gaattgttg 
        gggaaggat ggtaaagtt aggtgttct agtgatttg 
        gattacgtt gagcaattt ttgcgacgg ttgcaaaac 
        aagtgggag tttttatgc gagtcttac atcaaccta 
        acgataaac atcccagtg caagacagt gaagacgaa 
        ttgaagagt tctagtgat aatcttgac caaattgcc 
        gacaaaccc cgacctttc aaattagca gatttcaag 
        caagaagaa gacactttg agttcaagc actgattcg 
        gaaggttat ttggagaag gaaattttg ccaaatgtg 
        aaaactgaa gaggaggcg gaaaaaccg aaactgcgt 
        caaagttgt agtcttgac gagacaatt ttgaaaaag 
        acaaaggaa gtctcatcg actgatgac gaaacgaat 
        gacatggcg gccaaatat acgaaacag atccacgac 
        aagtataaa gtagagctt aattcaatt aaatacgac 
        actaatacg ttgctgcaa atgcgtaaa tcgaccatt 
        tcgagtgat aatatcacg agttgtgac agttttgag 
        aaaaagacg ccaagtccg aggattaat ccttttatg 
        gtggctaat gtgccaccg ccgcaaccg ataccgcca 
        gaaattcag caacagagg gccatgagt atctttaag 
        gactctgag gcgtacaag acgactcgg atcgagaca 
        atggaggca tgtctcgag cttttgagt agtctatcg 
        tctgaaaag ctctcgcct ttggccacc gttttgaaa 
        caaggcgcc gttcttcta atcggggct caggacgag 
        aaaataaaa aacgcagct gagaatctt ctcaacaga 
        atgaatatc tcgtacatt gaccacgat aatttctaa
      mRNA sequence
      • >TC006202 RA
        tactaatcg agtgcataa cgcaattta gtgaaaaca 
        tagcccgat taagcactt ttggacatt tttcgaatt 
        tttctctcg actaataat gtcgcaagt taaacagca 
        agagagtaa gaaaggggc gtttttgga tagagatga 
        ttttgcttc aaaactggt catcggacc ggttgatct 
        atgcatcga aagcctcaa actcctcca aaattgcgt 
        cgccatctt atcaatgat gtacaacat gccgaaatg 
        tccctcctt taacgtcac acatcgcat gaatattga 
        caccgatcc gcaagttgt tgatccaat catccagaa 
        tattgaaga gttacacga tttggaatt gtgcgaatc 
        aagtggtat tactttagc caaaatgga tcggcaaag 
        taacgtttt aaaaatttg gtacatcct ccttgaagg 
        actctcctc agcttctat catgtcagc tcataaaaa 
        ccaaccatt tgttgttga aaccctctt caaactttt 
        gagggattt gatcgataa cccgggcgt taactttgg 
        gttatattc cataaatca cttgctact cccgatggt 
        tcaaaactg gatgcgcca gaacagcaa gatccaaaa 
        accgggcga aatctggca aaacttcaa gcactgttg 
        tggcttagc tagtgagct tgtggtcga ttatttgca 
        ccatttgca aggtttcgg cccttttgt tgaaaaccc 
        agccgctac tttggccct caaacaact cctcttgat 
        cctaatcga gtgtataac acaacctag cgaaaacat 
        agcccggtt agggacttc ctgcatccg tcacgttat 
        tttaagtgc tggtaagat tttgactgg agggcaatg 
        ggaaattct ggtcgtggc cctgcacag ggcttgcac 
        catctcgct taaccctct ccaccaact aaccctcgc 
        atttaaaga gaactcgga tagttttta cggcgactt 
        ggcgacagt taaagtcgc agcgcgtaa agacagcac 
        agtgtccga aaacgagag gttgtacga gcttgccca 
        gcaagtttt cctcatgca atgccaacg caactgtct 
        caagtgcga caagttgtg cgagtgtga tgccagtat 
        gacgattga tgatcgcag ttatgtttt tctcctcta 
        tccgcttga catgtcttc gattttaaa cccagattt 
        gtatacagt gagggttcc atttcgcaa tttggtggt 
        tgggatttt ggcgcatca aatgctcgt tcagacgat 
        ttccagcgc gcttttgca aaatttgaa ccatatcac 
        gccgatttt gaacatttt gcaaatcaa ttcgtgtag 
        tttgagggg gattttgac gcatgttaa cgccaaaca 
        tgtcgatat tctgttgaa gtgtttgga tgtgagtaa 
        tgccagagc gaagcagtt tgatgtcag gcccggctt 
        gaagttttt gactcagaa ggttgggaa tttgagcaa 
        ggcggcgaa tttgtacac gcaggcaaa cacttcgga 
        ggtggtcca aatgattgt tttaatcga gttgtgtct 
        tgatggcat tgaaaaata ttcgattaa acccaaaga 
        cttgaaaga attgttggg gaaggatgg taaagttag 
        gtgttctag tgatttgga ttacgttga gcaattttt 
        gcgacggtt gcaaaacaa gtgggagtt tttatgcga 
        gtcttacat caacctaac gataaacat cccagtgca 
        agacagtga agacgaatt gaagagttc tagtgataa 
        tcttgacca aattgccga caaaccccg acctttcaa 
        attagcaga tttcaagca agaagaaga cactttgag 
        ttcaagcac tgattcgga aggttattt ggagaagga 
        aattttgcc aaatgtgaa aactgaaga ggaggcgga 
        aaaaccgaa actgcgtca aagttgtag tcttgacga 
        gacaatttt gaaaaagac aaaggaagt ctcatcgac 
        tgatgacga aacgaatga catggcggc caaatatac 
        gaaacagat ccacgacaa gtataaagt agagcttaa 
        ttcaattaa atacgacac taatacgtt gctgcaaat 
        gcgtaaatc gaccatttc gagtgataa tatcacgag 
        ttgtgacag ttttgagaa aaagacgcc aagtccgag 
        gattaatcc ttttatggt ggctaatgt gccaccgcc 
        gcaaccgat accgccaga aattcagca acagagggc 
        catgagtat ctttaagga ctctgaggc gtacaagac 
        gactcggat cgagacaat ggaggcatg tctcgagct 
        tttgagtag tctatcgtc tgaaaagct ctcgccttt 
        ggccaccgt tttgaaaca aggcgccgt tcttctaat 
        cggggctca ggacgagaa aataaaaaa cgcagctga 
        gaatcttct caacagaat gaatatctc gtacattga 
        ccacgataa tttctaatt tatttgcac tctttaaga 
        ctggatttg gaagtctga tggtaaatc ttcgtgctt 
        actttccgt ataattact gtagaataa aaaaataaa 
        tgtaaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY