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Gene information
General information
TC006188 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4754379..4762429
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006188
    iBeetle screen iB_04084

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006188 RA
      MYRLFNTRI SSFRKLTNL SSGFNSENV NFKVRDGGG 
      LLNTQLNNY CIACARNQS TNTNPNLAP KKIRKRSKS 
      KKSYYDLLV VTETNAKQQ KTSIKKLTW NDLKLLAST 
      NLNLSELHK LKDLTKDNQ HVQEHTYNN LAVIDPIAA 
      EETPNEKER VVTITDIPQ PPEQPQPPV EELLDTIPK 
      IDFLQPEII DEPPEISEV SDPAPKINL SKKARYEIS 
      VKTLGSYLE MCSTLQTPN RGLNALIFH NQRATRGKD 
      LTFATMKSI KVYNAVLKG FAAKGETSR LEEVLAMVR 
      KENVKLNLQ SYIAILECY GRANFHDHY LKQIRIYAK 
      EAVTNGFTF DRMLNEGTF LNDERAVVL ATMQKFDPS 
      YRPKYAEPI VQYDNELVN SLNHDSQLE LPEEGRLID 
      ETGLFTPGF MQSAVAKQL ELEKTGQIV VKSIENRGK 
      VSDEVTKYR EIMNQHIRD WEETAAKAF NRDLSALAA 
      QKSPLNPEI YMRSIPLKD FVTILVNEA KQIAEGSET 
      YSPTVNQLY RDLGSKVYA RYKILRKQK TGVLDKVQS 
      IHSRYCQSY AHQHEELGV LPETVHHVN PRQQWQWFE 
      YSMKGTGAT LTMDHRTWS PSTLQTIGK FLYKIVMHD 
      LKVDVNAMK ANIKHKNNH PAFYTVFRT QGRVLKEEV 
      KPHPVLARL FRASMPETL TFPANELPM FCPPVPWTC 
      VDNGGYLIV PCEVVRLPP QATSQKQRL YQSEVQQLY 
      PPLDALNQL AAVAWKVNT DVLDVILEV FNNGGSAKL 
      DVPEPPTAL ARPQAITND MDKAQKFKL FRQKLQYRR 
      KKAEMYSLW CDCLYRLSL ANHFRSDIF WLPHNMDFR 
      GRVYPVPPH LNHLGSDLA RSMLVFAES RPLGPDGLN 
      WLKLHLINL TGLKKRDSI TDRLAYADT IMDDILDSA 
      DSPLTGRQW WAESEEPWQ TLACCIEIA KATRSGNPE 
      TYRSHFPVH QDGSCNGLQ HYAALGRDS AGAYSVNLC 
      PSEVPQDVY SAVVALVEQ QRLVDAANG VEVAKALHG 
      FVKRKVIKQ TIMTTVYGV TKYGARLQI ARQLKDIDD 
      FPKDLVWAA SSYLTGRTF DSLRSMFTS TREIQDWFT 
      DCARLISMS GQHVEWVTP MGLPIVQPY IRYKNLGTS 
      LVYKPYHMD EFEKPNVMK QKNAFPPNF IHSLDSSHM 
      MLTSLFCER AGITFVSVH DCYWTHPST VDIMNKICR 
      EQFVALHSL PILENLSMF LTEKYSFRN DDLIGDGSI 
      SDLTKTKLN KVLRKLPKT GDFDITQVL KSVYFFS
    CDS sequence
    • >TC006188 RA
      atgtatcga ttattcaac acgcgaatt tcatctttc 
      cgaaagctg acaaatttg tcatcgggt tttaacagt 
      gaaaatgta aattttaag gttagggac gggggtggc 
      ctcctcaac acacagcta aataactac tgtattgca 
      tgtgctcgt aatcaatcg actaacact aacccgaac 
      ttagcgcct aaaaagata cgcaaaaga tcgaagtcg 
      aagaaatcg tattatgac ctacttgtt gtgactgag 
      acaaacgcc aaacaacaa aaaacctca atcaagaaa 
      ttaacctgg aacgacctc aaactcctc gcctcgacc 
      aacctcaac ctcagcgag ctccacaaa ctcaaagac 
      ctgaccaaa gacaaccaa cacgtccag gaacacacc 
      tacaacaac ctggcggtg atcgacccc atcgccgcc 
      gaggaaacc cccaatgag aaggaacgc gttgtcaca 
      atcaccgac atcccccag cccccggaa caaccccaa 
      cccccagtc gaggaatta ctcgacaca atccccaaa 
      atcgacttc ctccaaccc gaaatcatc gacgaaccg 
      ccagagata agcgaggtc agcgacccc gcacccaaa 
      ataaatttg agcaaaaaa gcgcgttac gaaatatca 
      gtcaaaaca ctaggctcg tacttggag atgtgctcc 
      accttgcaa acgcccaat cggggccta aacgccctc 
      atcttccac aatcagagg gccaccaga ggcaaggat 
      ttgactttc gccacaatg aaaagcatc aaggtttac 
      aacgcggta ctgaagggg ttcgccgcc aagggcgaa 
      acgtcgcga ctcgaggaa gtgctagcg atggtacgc 
      aaagagaac gtcaagctg aacttgcag agttatatt 
      gcaatattg gagtgttac ggacgggcc aatttccac 
      gaccactac ctcaaacaa attcggatt tatgcaaag 
      gaggccgtg actaacggt ttcaccttc gatcggatg 
      ctgaacgaa gggactttt ctcaacgac gagcgggcc 
      gttgttttg gcaacgatg caaaaattt gacccgtct 
      tatcgtcca aaatatgcg gaaccaatt gttcagtat 
      gacaatgaa ctggtcaat agcttgaac cacgatagt 
      caactggaa ttgcctgag gagggccgt ttgattgac 
      gaaactggg ctcttcacc cccggtttt atgcaatca 
      gcggttgcc aaacaactc gagttggaa aaaaccggt 
      caaatcgtc gtcaaaagt atcgaaaat cgtggcaaa 
      gtctcagac gaagtgaca aagtatcgc gagataatg 
      aaccagcac atcagagac tgggaggag accgctgcc 
      aaggctttc aaccgcgac ttgagcgcc ttggcggcc 
      cagaagagc cccctgaac cccgaaatc tacatgcgg 
      agcatcccc cttaaggac tttgtcacg atcctggtg 
      aacgaagcg aagcaaatt gccgagggt tccgaaacc 
      tacagtccg acagtgaac cagttgtac cgcgatttg 
      gggagcaag gtgtacgca aggtacaag atcttgcgt 
      aagcagaag accggtgtc ttggacaag gtccagtca 
      attcactcg cggtattgc caaagttac gcccaccag 
      cacgaagaa ctgggggtt ttacccgag acagtccac 
      cacgtgaac ccccggcag cagtggcag tggttcgag 
      tacagcatg aagggcact ggggccacc ctaaccatg 
      gaccaccgc acttggtcg ccctccacc ctccaaacc 
      atcggcaaa ttcctgtac aagatcgtg atgcacgac 
      ttgaaagtg gacgtcaac gccatgaag gccaacatc 
      aagcataag aacaaccac ccggcgttt tacaccgtg 
      ttccggacg caggggcgc gtcctgaag gaggaggtg 
      aagccgcat ccggtgttg gcgcggctg ttccgggcg 
      tcgatgccg gagacgttg acgtttccg gcgaatgag 
      ctgccgatg ttttgcccg cctgttccc tggacgtgt 
      gtggacaac ggcgggtat ttgattgtt ccgtgtgag 
      gttgtgcgg ttgccgccg caggccacg tcgcagaag 
      caacgcttg tatcagagt gaggttcag cagttgtat 
      ccgcctttg gatgcactg aatcagctg gcggcagtg 
      gcatggaag gttaatacc gatgttttg gatgtgatt 
      ttggaggtt tttaataat gggggctcg gcgaagttg 
      gatgtgccg gaaccgcca acggcgttg gctaggcca 
      caggcgatc acgaatgat atggataag gctcagaag 
      tttaagttg tttaggcag aagttgcag tataggagg 
      aagaaggcg gagatgtat tcgctgtgg tgcgattgt 
      ttgtacagg ttatcactg gccaatcat ttccggtcg 
      gacatattc tggctcccc cacaacatg gacttccgg 
      ggccgcgtc taccccgtc ccccctcac ttgaaccac 
      ctcggttcg gacttggca cgttcaatg ctagttttc 
      gccgaatca cgccctcta ggccccgac ggcctcaac 
      tggctcaaa ctccactta atcaacctc accggttta 
      aaaaaacgc gactcgata accgatcgg ttagcatac 
      gctgacacc atcatggac gacatcctc gattccgcc 
      gacagcccc ctcactggc cgccaatgg tgggcggaa 
      tcagaggag ccgtggcaa accctcgcc tgctgcatc 
      gaaatcgcc aaagcgacg cgttcgggg aaccccgag 
      acgtaccgc tcccacttc ccggtgcac caagacggc 
      agctgcaac ggcctgcaa cactacgcg gccttgggg 
      cgggacagc gcaggggcg tacagcgtg aatttgtgc 
      ccgtcggag gtgccccag gacgtgtac agcgccgtg 
      gtggcgctg gtggagcag cagcggctc gtggacgcg 
      gccaatggg gtggaagtc gcgaaggcg ctccatggg 
      ttcgttaag cgcaaagtg atcaagcag accataatg 
      accacggtt tatggggtg acgaagtac ggggccagg 
      ttgcaaatc gcgaggcag ttgaaggat attgacgat 
      ttcccgaag gatttggtc tgggcggct tcttcctac 
      ctcactgga cggactttt gactcgttg aggtcgatg 
      tttacgtcg acgagggag attcaagac tggtttacg 
      gactgtgcc cgcttgatt tctatgagt gggcagcac 
      gtcgaatgg gtgactccc atggggttg cccattgtg 
      cagccatac attaggtat aaaaatcta gggacgtct 
      ttggtctac aagccgtac catatggac gagtttgag 
      aaaccaaat gtgatgaaa caaaaaaat gcctttccg 
      cccaatttt atacattcg ctcgattct agtcatatg 
      atgctcacg tcgctgttt tgcgaacga gcggggatt 
      acgtttgtt tcggttcat gattgttac tggacgcat 
      ccttcgact gtagatatt atgaataaa atatgtagg 
      gaacaattt gtcgcactt cattcattg ccaatttta 
      gagaatctc tcgatgttt ttgaccgaa aagtacagt 
      tttcgaaat gacgatctt atcggagat ggatctata 
      tcagatttg accaaaacg aagctaaat aaggtgcta 
      aggaagtta ccaaagacg ggcgatttt gatataaca 
      caagtttta aaatctgtg tacttcttt agttaa
    mRNA sequence
    • >TC006188 RA
      tgcggttct tataagcac gtgaatgtc aaattcgaa 
      cgtttttta cacttttct accgatttt aacatgaaa 
      taacgactc gtcaaacat gtatcgatt attcaacac 
      gcgaatttc atctttccg aaagctgac aaatttgtc 
      atcgggttt taacagtga aaatgtaaa ttttaaggt 
      tagggacgg gggtggcct cctcaacac acagctaaa 
      taactactg tattgcatg tgctcgtaa tcaatcgac 
      taacactaa cccgaactt agcgcctaa aaagatacg 
      caaaagatc gaagtcgaa gaaatcgta ttatgacct 
      acttgttgt gactgagac aaacgccaa acaacaaaa 
      aacctcaat caagaaatt aacctggaa cgacctcaa 
      actcctcgc ctcgaccaa cctcaacct cagcgagct 
      ccacaaact caaagacct gaccaaaga caaccaaca 
      cgtccagga acacaccta caacaacct ggcggtgat 
      cgaccccat cgccgccga ggaaacccc caatgagaa 
      ggaacgcgt tgtcacaat caccgacat cccccagcc 
      cccggaaca accccaacc cccagtcga ggaattact 
      cgacacaat ccccaaaat cgacttcct ccaacccga 
      aatcatcga cgaaccgcc agagataag cgaggtcag 
      cgaccccgc acccaaaat aaatttgag caaaaaagc 
      gcgttacga aatatcagt caaaacact aggctcgta 
      cttggagat gtgctccac cttgcaaac gcccaatcg 
      gggcctaaa cgccctcat cttccacaa tcagagggc 
      caccagagg caaggattt gactttcgc cacaatgaa 
      aagcatcaa ggtttacaa cgcggtact gaaggggtt 
      cgccgccaa gggcgaaac gtcgcgact cgaggaagt 
      gctagcgat ggtacgcaa agagaacgt caagctgaa 
      cttgcagag ttatattgc aatattgga gtgttacgg 
      acgggccaa tttccacga ccactacct caaacaaat 
      tcggattta tgcaaagga ggccgtgac taacggttt 
      caccttcga tcggatgct gaacgaagg gacttttct 
      caacgacga gcgggccgt tgttttggc aacgatgca 
      aaaatttga cccgtctta tcgtccaaa atatgcgga 
      accaattgt tcagtatga caatgaact ggtcaatag 
      cttgaacca cgatagtca actggaatt gcctgagga 
      gggccgttt gattgacga aactgggct cttcacccc 
      cggttttat gcaatcagc ggttgccaa acaactcga 
      gttggaaaa aaccggtca aatcgtcgt caaaagtat 
      cgaaaatcg tggcaaagt ctcagacga agtgacaaa 
      gtatcgcga gataatgaa ccagcacat cagagactg 
      ggaggagac cgctgccaa ggctttcaa ccgcgactt 
      gagcgcctt ggcggccca gaagagccc cctgaaccc 
      cgaaatcta catgcggag catccccct taaggactt 
      tgtcacgat cctggtgaa cgaagcgaa gcaaattgc 
      cgagggttc cgaaaccta cagtccgac agtgaacca 
      gttgtaccg cgatttggg gagcaaggt gtacgcaag 
      gtacaagat cttgcgtaa gcagaagac cggtgtctt 
      ggacaaggt ccagtcaat tcactcgcg gtattgcca 
      aagttacgc ccaccagca cgaagaact gggggtttt 
      acccgagac agtccacca cgtgaaccc ccggcagca 
      gtggcagtg gttcgagta cagcatgaa gggcactgg 
      ggccaccct aaccatgga ccaccgcac ttggtcgcc 
      ctccaccct ccaaaccat cggcaaatt cctgtacaa 
      gatcgtgat gcacgactt gaaagtgga cgtcaacgc 
      catgaaggc caacatcaa gcataagaa caaccaccc 
      ggcgtttta caccgtgtt ccggacgca ggggcgcgt 
      cctgaagga ggaggtgaa gccgcatcc ggtgttggc 
      gcggctgtt ccgggcgtc gatgccgga gacgttgac 
      gtttccggc gaatgagct gccgatgtt ttgcccgcc 
      tgttccctg gacgtgtgt ggacaacgg cgggtattt 
      gattgttcc gtgtgaggt tgtgcggtt gccgccgca 
      ggccacgtc gcagaagca acgcttgta tcagagtga 
      ggttcagca gttgtatcc gcctttgga tgcactgaa 
      tcagctggc ggcagtggc atggaaggt taataccga 
      tgttttgga tgtgatttt ggaggtttt taataatgg 
      gggctcggc gaagttgga tgtgccgga accgccaac 
      ggcgttggc taggccaca ggcgatcac gaatgatat 
      ggataaggc tcagaagtt taagttgtt taggcagaa 
      gttgcagta taggaggaa gaaggcgga gatgtattc 
      gctgtggtg cgattgttt gtacaggtt atcactggc 
      caatcattt ccggtcgga catattctg gctccccca 
      caacatgga cttccgggg ccgcgtcta ccccgtccc 
      ccctcactt gaaccacct cggttcgga cttggcacg 
      ttcaatgct agttttcgc cgaatcacg ccctctagg 
      ccccgacgg cctcaactg gctcaaact ccacttaat 
      caacctcac cggtttaaa aaaacgcga ctcgataac 
      cgatcggtt agcatacgc tgacaccat catggacga 
      catcctcga ttccgccga cagccccct cactggccg 
      ccaatggtg ggcggaatc agaggagcc gtggcaaac 
      cctcgcctg ctgcatcga aatcgccaa agcgacgcg 
      ttcggggaa ccccgagac gtaccgctc ccacttccc 
      ggtgcacca agacggcag ctgcaacgg cctgcaaca 
      ctacgcggc cttggggcg ggacagcgc aggggcgta 
      cagcgtgaa tttgtgccc gtcggaggt gccccagga 
      cgtgtacag cgccgtggt ggcgctggt ggagcagca 
      gcggctcgt ggacgcggc caatggggt ggaagtcgc 
      gaaggcgct ccatgggtt cgttaagcg caaagtgat 
      caagcagac cataatgac cacggttta tggggtgac 
      gaagtacgg ggccaggtt gcaaatcgc gaggcagtt 
      gaaggatat tgacgattt cccgaagga tttggtctg 
      ggcggcttc ttcctacct cactggacg gacttttga 
      ctcgttgag gtcgatgtt tacgtcgac gagggagat 
      tcaagactg gtttacgga ctgtgcccg cttgatttc 
      tatgagtgg gcagcacgt cgaatgggt gactcccat 
      ggggttgcc cattgtgca gccatacat taggtataa 
      aaatctagg gacgtcttt ggtctacaa gccgtacca 
      tatggacga gtttgagaa accaaatgt gatgaaaca 
      aaaaaatgc ctttccgcc caattttat acattcgct 
      cgattctag tcatatgat gctcacgtc gctgttttg 
      cgaacgagc ggggattac gtttgtttc ggttcatga 
      ttgttactg gacgcatcc ttcgactgt agatattat 
      gaataaaat atgtaggga acaatttgt cgcacttca 
      ttcattgcc aattttaga gaatctctc gatgttttt 
      gaccgaaaa gtacagttt tcgaaatga cgatcttat 
      cggagatgg atctatatc agatttgac caaaacgaa 
      gctaaataa ggtgctaag gaagttacc aaagacggg 
      cgattttga tataacaca agttttaaa atctgtgta 
      cttctttag ttaacttta agttaataa agtgatttt 
      tgttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY