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Gene information
General information
TC006187 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 4384008..4435381
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006187
    iBeetle screen iB_04083
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    head orientation irregular - Number of animals showing the phenotype: 8
    labrum swollen - Number of animals showing the phenotype: 8

    labrum shape irregular - Number of animals showing the phenotype: 8
    head size decreased - Number of animals showing the phenotype: 8
    legs size shortened - Number of animals showing the phenotype: 8

    pronotum orientation bent - Number of animals showing the phenotype: 8

    Comment: Transverse furrow present.
    elytra ventralnot closed - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006187 RA
      MTEVASLLP TDHRLTTVR RYEAGPPEG LELVEPGET 
      YATTKEEEW VSKKKTEVI NTKQIETRV KRQVVLEDG 
      EVVEDSGPM VTTNTTEDT ETQEHHQTE LRKLGDDEP 
      DGPSKAVEG TPEVPSDGS KWVAVPNPE GVVREVKEK 
      RVISREETE EVKETEDVQ HFGDITDED FLSAVKTGK 
      KDLRDVLRT TKNGVSVVS TGPRIVHES SKSNKVTDT 
      ENTRELSSV LPDGKIVTE TQRTTEHEE IKDDELPED 
      APEQDFYKE NSQRYLKTR DQEDVDYLA DGVKIGHEM 
      RFRTETMEV ERHGDDDDE PDFDSLSAR ARRRANNRQ 
      PHRYRVPIT GLENGVSPL DRKDALTRK PLDFDQEEE 
      TRKVETSKW LEHHFGSES RSSNSVIDD EEQPPKTSF 
      FNVTIKSQP SRAEPPPQK YITTINNTP RVYSPVEPE 
      RDRPTHHSD YYKGISQWS ERHPMPSYH HQDTPIYVH 
      EPVTYTPPR RSPIPDYRS SSSPMLVPP PVRVSPIRE 
      ITPTPPQRK RVSERRHQQ DSRYANAME PPPDYSPPV 
      NEKKAMQKT RFAPDPPKA KSGNIIGQS IRKLVGKIR 
      SASTERKTR HRTKRSPSP SYQKGHVID NNIHHNGVE 
      SKQPVQRYY LGEDPFGGS IYGRENKYD GVKPARGSS 
      KRKEEEHRQ CRVRGVGMP HISRCLNCP NHSRSQSTL 
      GRFSKSTSR LLNAPAPHE RNSQTLPRH LSRHHEPPT 
      RLEKNNKSN STINVSIIN TVSRTLGPP LGPAKPART 
      YKSNLSRSK SLNVHANDY RKPGMYTSN PHLNRLEEN 
      PVGLKSPGL ISSLSRSQK DLQEDSVYT NRFVRNGTS 
      DNTSDNKRV FMKSLKDRA PELFKTLHE DEPDSVIYS 
      TPIKNHHKN KYNGDIYEP PTRLREPQS ILRRGSNSS 
      TDYSETYHT TTRNDDPLR PSVTNTVKS YSKKTIPAK 
      NGRSMETIE SSETKSVTK SHYRGDPSV KFYETERRY 
      SGGSPVVIE VRNSNYRK
    • >TC006187 RB
      MTEVASLLP TDHRLTTVR RYEAGPPEG LELVEPGET 
      YATTKEEEW VSKKKTEVI NTKQIETRV KRQVVLEDG 
      EVVEDSGPM VTTNTTEDT ETQEHHQTE LRKLGDDEP 
      DGPSKAVEG TPEVPSDGS KWVAVPNPE GVVREVKEK 
      RVISREETE EVKETEDVQ HFGDITDED FLSAVKTGK 
      KDLRDVLRT TKNGVSVVS TGPRIVHES SKSNKVTDT 
      ENTRELSSV LPDGKIVTE TQRTTEHEE IKDDELPED 
      APEQDFYKE NSQRYLKTR DQEDVDYLA DGVKIGHEM 
      RFRTETMEV ERHGDDDDE PDFDSLSAR ARRRANNRQ 
      PHRYRGLEN GVSPLDRKD ALTRKPLDF DQEEETRKV 
      ETSKWLEHH FGSESRSSN SVIDDEEQP PKTSFFNVT 
      IKSQPSRAE PPPQKYITT INNTPRVYS PVEPERDRP 
      THHSDYYKG ISQWSERHP MPSYHHQDT PIYVHEPVT 
      YTPPRRSPI PDYRSSSSP MLVPPPVRV SPIREITPT 
      PPQRKRVSE RRHQQDSRY ANAMEPPPD YSPPVNEKK 
      AMQKTRFAP DPPKAKSGN IIGQSIRKL VGKIRSAST 
      ERKTRHRTK RSPSPSYQK GHVIDNNIH HNGVESKQP 
      VQRYYLGED PFGGSIYGR ENKYDGVKP ARGSSKRKE 
      EEHRQCRVR GVGMPHISR CLNCPNHSR SQSTLGRFS 
      KSTSRLLNA PAPHERNSQ TLPRHLSRH HEPPTRLEK 
      NNKSNSTIN VSIINTVSR TLGPPLGPA KPARTYKSN 
      LSRSKSLNV HANDYRKPG MYTSNPHLN RLEENPVGL 
      KSPGLISSL SRSQKDLQE DSVYTNRFV RNGTSDNTS 
      DNKRVFMKS LKDRAPELF KTLHEDEPD SVIYSTPIK 
      NHHKNKYNG DIYEPPTRL REPQSILRR GSNSSTDYS 
      ETYHTTTRN DDPLRPSVT NTVKSYSKK TIPAKNGRS 
      METIESSET KSVTKSHYR GDPSVKFYE TERRYSGGS 
      PVVIEVRNS NYRK
    CDS sequence
    • >TC006187 RA
      atgacggaa gttgcaagt ctcctgcca acggaccat 
      agactgacc acggtccgg cggtatgag gccggacca 
      ccggaaggc ctggagctg gttgagccg ggggagacc 
      tatgccacc accaaagaa gaggaatgg gtgagcaaa 
      aagaaaacg gaagtaatc aacaccaaa caaatcgag 
      acgcgagtc aaacgccaa gtggtcctc gaagatggc 
      gaagtcgtc gaagactcc ggacccatg gtcaccacc 
      aacaccact gaagacacc gaaacgcaa gaacaccac 
      cagactgaa ttacgcaaa ctgggcgac gacgaaccc 
      gatggcccc tccaaggcc gtggagggc acccccgag 
      gtgcccagc gatgggtcc aaatgggtg gcggtgccc 
      aaccccgaa ggggtcgtc cgcgaggtt aaggagaag 
      cgagtcatc tcacgcgag gaaaccgaa gaggttaag 
      gaaacggaa gacgttcag cacttcgga gacatcacc 
      gatgaggat ttcctctca gctgtaaaa accggaaaa 
      aaggatctc cgcgacgtc ctccgtacg acgaaaaac 
      ggcgtttcg gtggtttct accggccct cgaatcgtc 
      cacgagtcg tcaaagagc aacaaagtg acagatacg 
      gaaaacacg cgtgaactg agctcggta ctacctgac 
      ggtaaaatc gtgaccgag acgcaaaga accaccgaa 
      cacgaagag atcaaagac gacgaattg cccgaagac 
      gcgcccgaa caagatttc tacaaggag aattcgcaa 
      aggtatctg aaaacacgc gatcaggaa gatgtggat 
      tacctagct gacggtgtt aaaatcggg cacgaaatg 
      cgcttccgg accgagacc atggaggtt gaaaggcat 
      ggagatgat gacgatgag cccgatttc gattcgttg 
      agtgccagg gcgaggagg agggccaac aaccgacaa 
      ccgcacaga tacagggtc ccgattaca ggtctggag 
      aacggtgtt tccccattg gaccgcaaa gacgccctg 
      acccgaaaa ccgctcgat ttcgaccaa gaggaagaa 
      acgcgaaaa gtggaaaca tcgaaatgg ttagagcac 
      catttcggg agcgaatca aggtcttcg aattccgtc 
      atagacgac gaagaacag ccaccgaaa acaagtttc 
      tttaatgtc acaatcaaa tcgcagccg agtcgagcc 
      gaaccacca cctcagaaa tacatcaca acgataaac 
      aacactccg cgagtgtac agtccggtg gagcccgag 
      cgcgaccgc cccacacat cactccgac tactacaaa 
      ggtatcagt cagtggtcg gagcgccat cccatgccc 
      tcctaccac caccaggac accccgatc tacgtgcac 
      gagcccgtg acgtacacg cccccacgc cggtccccc 
      atccccgac taccgcagc agcagctcg cccatgctg 
      gtgcctcct ccagtccgc gtctccccc atccgggag 
      atcactccg acgccccca cagcgcaaa cgggtttcc 
      gagcgtcga caccagcaa gacagtcga tacgcgaac 
      gcgatggag cctccaccg gactactcg ccgccggtc 
      aacgagaag aaggccatg cagaaaacg cgcttcgcc 
      ccggacccc cccaaggcc aaaagcggc aacatcata 
      ggccagtcc atccggaaa ctagtcggg aaaattcga 
      tcggcgagc accgaacgc aagaccagg cacaggacg 
      aagaggtcc cccagcccc tcgtaccaa aagggccac 
      gtgatcgac aacaacatc caccacaac ggcgtcgag 
      agcaagcag ccggtgcag aggtactac ctgggggag 
      gaccccttc gggggcagc atctacggc agggagaac 
      aagtacgac ggggtgaag ccggccaga ggcagctcc 
      aagaggaaa gaagaggag cacaggcaa tgccgtgtc 
      cgcggcgtg ggcatgccc catataagc cgatgtcta 
      aactgtccc aaccattcc aggtcccag agtaccctg 
      ggccgcttc agtaaatcc accagtcgc cttctgaac 
      gcgccagcc ccccacgag cgcaactcg cagacgctc 
      ccgcgtcac ctgtcccgc caccacgag cccccgacc 
      cgtctggag aagaacaac aagtccaac agcacgatc 
      aacgtctcc atcatcaac actgtgtcg cgcaccctt 
      ggcccacct ctgggcccc gccaagcca gccaggaca 
      tacaaatcg aatctctca cgcagcaaa agtttgaac 
      gtccacgcc aacgattac cgcaagccg gggatgtac 
      accagcaac ccccatctc aatcggctg gaggagaac 
      ccagtcggg ctcaaaagt cctggattg atttccagc 
      cttagtcgc agccagaag gacctccag gaagacagt 
      gtgtacacg aaccgtttt gtaagaaac ggcaccagt 
      gacaacacc agtgataat aaacgagtg ttcatgaaa 
      agtctcaag gatcgagcg ccggaattg tttaaaact 
      ttgcacgag gacgagcca gactctgtg atctacagc 
      accccaatc aaaaaccac cacaagaac aagtacaat 
      ggggacatc tacgagccc ccgacgcgc ctgagggag 
      ccccagtcc atcctcagg agagggagc aactccagc 
      accgactac tccgaaacg taccacacc accacccga 
      aacgacgac cccctcaga cccagcgtc accaacacg 
      gtgaagagc tacagcaag aagacaatc ccggcgaaa 
      aacgggcga agcatggag acgatcgag tcaagcgag 
      acgaagtcg gtcaccaaa agccactat cggggggat 
      cccagcgtg aagttctac gagaccgag cggagatac 
      agcggggga agccccgta gtcatcgaa gtccgaaat 
      agcaactat aggaaatga
    • >TC006187 RB
      atgacggaa gttgcaagt ctcctgcca acggaccat 
      agactgacc acggtccgg cggtatgag gccggacca 
      ccggaaggc ctggagctg gttgagccg ggggagacc 
      tatgccacc accaaagaa gaggaatgg gtgagcaaa 
      aagaaaacg gaagtaatc aacaccaaa caaatcgag 
      acgcgagtc aaacgccaa gtggtcctc gaagatggc 
      gaagtcgtc gaagactcc ggacccatg gtcaccacc 
      aacaccact gaagacacc gaaacgcaa gaacaccac 
      cagactgaa ttacgcaaa ctgggcgac gacgaaccc 
      gatggcccc tccaaggcc gtggagggc acccccgag 
      gtgcccagc gatgggtcc aaatgggtg gcggtgccc 
      aaccccgaa ggggtcgtc cgcgaggtt aaggagaag 
      cgagtcatc tcacgcgag gaaaccgaa gaggttaag 
      gaaacggaa gacgttcag cacttcgga gacatcacc 
      gatgaggat ttcctctca gctgtaaaa accggaaaa 
      aaggatctc cgcgacgtc ctccgtacg acgaaaaac 
      ggcgtttcg gtggtttct accggccct cgaatcgtc 
      cacgagtcg tcaaagagc aacaaagtg acagatacg 
      gaaaacacg cgtgaactg agctcggta ctacctgac 
      ggtaaaatc gtgaccgag acgcaaaga accaccgaa 
      cacgaagag atcaaagac gacgaattg cccgaagac 
      gcgcccgaa caagatttc tacaaggag aattcgcaa 
      aggtatctg aaaacacgc gatcaggaa gatgtggat 
      tacctagct gacggtgtt aaaatcggg cacgaaatg 
      cgcttccgg accgagacc atggaggtt gaaaggcat 
      ggagatgat gacgatgag cccgatttc gattcgttg 
      agtgccagg gcgaggagg agggccaac aaccgacaa 
      ccgcacaga tacaggggt ctggagaac ggtgtttcc 
      ccattggac cgcaaagac gccctgacc cgaaaaccg 
      ctcgatttc gaccaagag gaagaaacg cgaaaagtg 
      gaaacatcg aaatggtta gagcaccat ttcgggagc 
      gaatcaagg tcttcgaat tccgtcata gacgacgaa 
      gaacagcca ccgaaaaca agtttcttt aatgtcaca 
      atcaaatcg cagccgagt cgagccgaa ccaccacct 
      cagaaatac atcacaacg ataaacaac actccgcga 
      gtgtacagt ccggtggag cccgagcgc gaccgcccc 
      acacatcac tccgactac tacaaaggt atcagtcag 
      tggtcggag cgccatccc atgccctcc taccaccac 
      caggacacc ccgatctac gtgcacgag cccgtgacg 
      tacacgccc ccacgccgg tcccccatc cccgactac 
      cgcagcagc agctcgccc atgctggtg cctcctcca 
      gtccgcgtc tcccccatc cgggagatc actccgacg 
      cccccacag cgcaaacgg gtttccgag cgtcgacac 
      cagcaagac agtcgatac gcgaacgcg atggagcct 
      ccaccggac tactcgccg ccggtcaac gagaagaag 
      gccatgcag aaaacgcgc ttcgccccg gaccccccc 
      aaggccaaa agcggcaac atcataggc cagtccatc 
      cggaaacta gtcgggaaa attcgatcg gcgagcacc 
      gaacgcaag accaggcac aggacgaag aggtccccc 
      agcccctcg taccaaaag ggccacgtg atcgacaac 
      aacatccac cacaacggc gtcgagagc aagcagccg 
      gtgcagagg tactacctg ggggaggac cccttcggg 
      ggcagcatc tacggcagg gagaacaag tacgacggg 
      gtgaagccg gccagaggc agctccaag aggaaagaa 
      gaggagcac aggcaatgc cgtgtccgc ggcgtgggc 
      atgccccat ataagccga tgtctaaac tgtcccaac 
      cattccagg tcccagagt accctgggc cgcttcagt 
      aaatccacc agtcgcctt ctgaacgcg ccagccccc 
      cacgagcgc aactcgcag acgctcccg cgtcacctg 
      tcccgccac cacgagccc ccgacccgt ctggagaag 
      aacaacaag tccaacagc acgatcaac gtctccatc 
      atcaacact gtgtcgcgc acccttggc ccacctctg 
      ggccccgcc aagccagcc aggacatac aaatcgaat 
      ctctcacgc agcaaaagt ttgaacgtc cacgccaac 
      gattaccgc aagccgggg atgtacacc agcaacccc 
      catctcaat cggctggag gagaaccca gtcgggctc 
      aaaagtcct ggattgatt tccagcctt agtcgcagc 
      cagaaggac ctccaggaa gacagtgtg tacacgaac 
      cgttttgta agaaacggc accagtgac aacaccagt 
      gataataaa cgagtgttc atgaaaagt ctcaaggat 
      cgagcgccg gaattgttt aaaactttg cacgaggac 
      gagccagac tctgtgatc tacagcacc ccaatcaaa 
      aaccaccac aagaacaag tacaatggg gacatctac 
      gagcccccg acgcgcctg agggagccc cagtccatc 
      ctcaggaga gggagcaac tccagcacc gactactcc 
      gaaacgtac cacaccacc acccgaaac gacgacccc 
      ctcagaccc agcgtcacc aacacggtg aagagctac 
      agcaagaag acaatcccg gcgaaaaac gggcgaagc 
      atggagacg atcgagtca agcgagacg aagtcggtc 
      accaaaagc cactatcgg ggggatccc agcgtgaag 
      ttctacgag accgagcgg agatacagc gggggaagc 
      cccgtagtc atcgaagtc cgaaatagc aactatagg 
      aaatga
    mRNA sequence
    • >TC006187 RA
      gatctgtag gcacaaggc gaggctcaa acacgctgc 
      gacactcgc aggaaacgt atctctgct cttgcagga 
      acgatagtc gcgctgtga ttcgtgtgc agtgacagt 
      catggtggt tttacccaa aaagcggga atattaaac 
      gagatgacg gaagttgca agtctcctg ccaacggac 
      catagactg accacggtc cggcggtat gaggccgga 
      ccaccggaa ggcctggag ctggttgag ccgggggag 
      acctatgcc accaccaaa gaagaggaa tgggtgagc 
      aaaaagaaa acggaagta atcaacacc aaacaaatc 
      gagacgcga gtcaaacgc caagtggtc ctcgaagat 
      ggcgaagtc gtcgaagac tccggaccc atggtcacc 
      accaacacc actgaagac accgaaacg caagaacac 
      caccagact gaattacgc aaactgggc gacgacgaa 
      cccgatggc ccctccaag gccgtggag ggcaccccc 
      gaggtgccc agcgatggg tccaaatgg gtggcggtg 
      cccaacccc gaaggggtc gtccgcgag gttaaggag 
      aagcgagtc atctcacgc gaggaaacc gaagaggtt 
      aaggaaacg gaagacgtt cagcacttc ggagacatc 
      accgatgag gatttcctc tcagctgta aaaaccgga 
      aaaaaggat ctccgcgac gtcctccgt acgacgaaa 
      aacggcgtt tcggtggtt tctaccggc cctcgaatc 
      gtccacgag tcgtcaaag agcaacaaa gtgacagat 
      acggaaaac acgcgtgaa ctgagctcg gtactacct 
      gacggtaaa atcgtgacc gagacgcaa agaaccacc 
      gaacacgaa gagatcaaa gacgacgaa ttgcccgaa 
      gacgcgccc gaacaagat ttctacaag gagaattcg 
      caaaggtat ctgaaaaca cgcgatcag gaagatgtg 
      gattaccta gctgacggt gttaaaatc gggcacgaa 
      atgcgcttc cggaccgag accatggag gttgaaagg 
      catggagat gatgacgat gagcccgat ttcgattcg 
      ttgagtgcc agggcgagg aggagggcc aacaaccga 
      caaccgcac agatacagg gtcccgatt acaggtctg 
      gagaacggt gtttcccca ttggaccgc aaagacgcc 
      ctgacccga aaaccgctc gatttcgac caagaggaa 
      gaaacgcga aaagtggaa acatcgaaa tggttagag 
      caccatttc gggagcgaa tcaaggtct tcgaattcc 
      gtcatagac gacgaagaa cagccaccg aaaacaagt 
      ttctttaat gtcacaatc aaatcgcag ccgagtcga 
      gccgaacca ccacctcag aaatacatc acaacgata 
      aacaacact ccgcgagtg tacagtccg gtggagccc 
      gagcgcgac cgccccaca catcactcc gactactac 
      aaaggtatc agtcagtgg tcggagcgc catcccatg 
      ccctcctac caccaccag gacaccccg atctacgtg 
      cacgagccc gtgacgtac acgccccca cgccggtcc 
      cccatcccc gactaccgc agcagcagc tcgcccatg 
      ctggtgcct cctccagtc cgcgtctcc cccatccgg 
      gagatcact ccgacgccc ccacagcgc aaacgggtt 
      tccgagcgt cgacaccag caagacagt cgatacgcg 
      aacgcgatg gagcctcca ccggactac tcgccgccg 
      gtcaacgag aagaaggcc atgcagaaa acgcgcttc 
      gccccggac ccccccaag gccaaaagc ggcaacatc 
      ataggccag tccatccgg aaactagtc gggaaaatt 
      cgatcggcg agcaccgaa cgcaagacc aggcacagg 
      acgaagagg tcccccagc ccctcgtac caaaagggc 
      cacgtgatc gacaacaac atccaccac aacggcgtc 
      gagagcaag cagccggtg cagaggtac tacctgggg 
      gaggacccc ttcgggggc agcatctac ggcagggag 
      aacaagtac gacggggtg aagccggcc agaggcagc 
      tccaagagg aaagaagag gagcacagg caatgccgt 
      gtccgcggc gtgggcatg ccccatata agccgatgt 
      ctaaactgt cccaaccat tccaggtcc cagagtacc 
      ctgggccgc ttcagtaaa tccaccagt cgccttctg 
      aacgcgcca gccccccac gagcgcaac tcgcagacg 
      ctcccgcgt cacctgtcc cgccaccac gagcccccg 
      acccgtctg gagaagaac aacaagtcc aacagcacg 
      atcaacgtc tccatcatc aacactgtg tcgcgcacc 
      cttggccca cctctgggc cccgccaag ccagccagg 
      acatacaaa tcgaatctc tcacgcagc aaaagtttg 
      aacgtccac gccaacgat taccgcaag ccggggatg 
      tacaccagc aacccccat ctcaatcgg ctggaggag 
      aacccagtc gggctcaaa agtcctgga ttgatttcc 
      agccttagt cgcagccag aaggacctc caggaagac 
      agtgtgtac acgaaccgt tttgtaaga aacggcacc 
      agtgacaac accagtgat aataaacga gtgttcatg 
      aaaagtctc aaggatcga gcgccggaa ttgtttaaa 
      actttgcac gaggacgag ccagactct gtgatctac 
      agcacccca atcaaaaac caccacaag aacaagtac 
      aatggggac atctacgag cccccgacg cgcctgagg 
      gagccccag tccatcctc aggagaggg agcaactcc 
      agcaccgac tactccgaa acgtaccac accaccacc 
      cgaaacgac gaccccctc agacccagc gtcaccaac 
      acggtgaag agctacagc aagaagaca atcccggcg 
      aaaaacggg cgaagcatg gagacgatc gagtcaagc 
      gagacgaag tcggtcacc aaaagccac tatcggggg 
      gatcccagc gtgaagttc tacgagacc gagcggaga 
      tacagcggg ggaagcccc gtagtcatc gaagtccga 
      aatagcaac tataggaaa tgattaact cgaagatac 
      tgccatata aactgtcat tatttataa ttgttatta 
      ctaattgtg attgttttg tcgttaaaa agggttttg 
      gcattgtta ggatggtag attaggtgc taaaattcc 
      ctaaacttt tgtatcata tgttgtttc taacgtaat 
      aataaatgg aaagaataa tcctagagg tatagaatg 
      tgccaaaat aatttgtaa atacgagc
    • >TC006187 RB
      gatctgtag gcacaaggc gaggctcaa acacgctgc 
      gacactcgc aggaaacgt atctctgct cttgcagga 
      acgatagtc gcgctgtga ttcgtgtgc agtgacagt 
      catggtggt tttacccaa aaagcggga atattaaac 
      gagatgacg gaagttgca agtctcctg ccaacggac 
      catagactg accacggtc cggcggtat gaggccgga 
      ccaccggaa ggcctggag ctggttgag ccgggggag 
      acctatgcc accaccaaa gaagaggaa tgggtgagc 
      aaaaagaaa acggaagta atcaacacc aaacaaatc 
      gagacgcga gtcaaacgc caagtggtc ctcgaagat 
      ggcgaagtc gtcgaagac tccggaccc atggtcacc 
      accaacacc actgaagac accgaaacg caagaacac 
      caccagact gaattacgc aaactgggc gacgacgaa 
      cccgatggc ccctccaag gccgtggag ggcaccccc 
      gaggtgccc agcgatggg tccaaatgg gtggcggtg 
      cccaacccc gaaggggtc gtccgcgag gttaaggag 
      aagcgagtc atctcacgc gaggaaacc gaagaggtt 
      aaggaaacg gaagacgtt cagcacttc ggagacatc 
      accgatgag gatttcctc tcagctgta aaaaccgga 
      aaaaaggat ctccgcgac gtcctccgt acgacgaaa 
      aacggcgtt tcggtggtt tctaccggc cctcgaatc 
      gtccacgag tcgtcaaag agcaacaaa gtgacagat 
      acggaaaac acgcgtgaa ctgagctcg gtactacct 
      gacggtaaa atcgtgacc gagacgcaa agaaccacc 
      gaacacgaa gagatcaaa gacgacgaa ttgcccgaa 
      gacgcgccc gaacaagat ttctacaag gagaattcg 
      caaaggtat ctgaaaaca cgcgatcag gaagatgtg 
      gattaccta gctgacggt gttaaaatc gggcacgaa 
      atgcgcttc cggaccgag accatggag gttgaaagg 
      catggagat gatgacgat gagcccgat ttcgattcg 
      ttgagtgcc agggcgagg aggagggcc aacaaccga 
      caaccgcac agatacagg ggtctggag aacggtgtt 
      tccccattg gaccgcaaa gacgccctg acccgaaaa 
      ccgctcgat ttcgaccaa gaggaagaa acgcgaaaa 
      gtggaaaca tcgaaatgg ttagagcac catttcggg 
      agcgaatca aggtcttcg aattccgtc atagacgac 
      gaagaacag ccaccgaaa acaagtttc tttaatgtc 
      acaatcaaa tcgcagccg agtcgagcc gaaccacca 
      cctcagaaa tacatcaca acgataaac aacactccg 
      cgagtgtac agtccggtg gagcccgag cgcgaccgc 
      cccacacat cactccgac tactacaaa ggtatcagt 
      cagtggtcg gagcgccat cccatgccc tcctaccac 
      caccaggac accccgatc tacgtgcac gagcccgtg 
      acgtacacg cccccacgc cggtccccc atccccgac 
      taccgcagc agcagctcg cccatgctg gtgcctcct 
      ccagtccgc gtctccccc atccgggag atcactccg 
      acgccccca cagcgcaaa cgggtttcc gagcgtcga 
      caccagcaa gacagtcga tacgcgaac gcgatggag 
      cctccaccg gactactcg ccgccggtc aacgagaag 
      aaggccatg cagaaaacg cgcttcgcc ccggacccc 
      cccaaggcc aaaagcggc aacatcata ggccagtcc 
      atccggaaa ctagtcggg aaaattcga tcggcgagc 
      accgaacgc aagaccagg cacaggacg aagaggtcc 
      cccagcccc tcgtaccaa aagggccac gtgatcgac 
      aacaacatc caccacaac ggcgtcgag agcaagcag 
      ccggtgcag aggtactac ctgggggag gaccccttc 
      gggggcagc atctacggc agggagaac aagtacgac 
      ggggtgaag ccggccaga ggcagctcc aagaggaaa 
      gaagaggag cacaggcaa tgccgtgtc cgcggcgtg 
      ggcatgccc catataagc cgatgtcta aactgtccc 
      aaccattcc aggtcccag agtaccctg ggccgcttc 
      agtaaatcc accagtcgc cttctgaac gcgccagcc 
      ccccacgag cgcaactcg cagacgctc ccgcgtcac 
      ctgtcccgc caccacgag cccccgacc cgtctggag 
      aagaacaac aagtccaac agcacgatc aacgtctcc 
      atcatcaac actgtgtcg cgcaccctt ggcccacct 
      ctgggcccc gccaagcca gccaggaca tacaaatcg 
      aatctctca cgcagcaaa agtttgaac gtccacgcc 
      aacgattac cgcaagccg gggatgtac accagcaac 
      ccccatctc aatcggctg gaggagaac ccagtcggg 
      ctcaaaagt cctggattg atttccagc cttagtcgc 
      agccagaag gacctccag gaagacagt gtgtacacg 
      aaccgtttt gtaagaaac ggcaccagt gacaacacc 
      agtgataat aaacgagtg ttcatgaaa agtctcaag 
      gatcgagcg ccggaattg tttaaaact ttgcacgag 
      gacgagcca gactctgtg atctacagc accccaatc 
      aaaaaccac cacaagaac aagtacaat ggggacatc 
      tacgagccc ccgacgcgc ctgagggag ccccagtcc 
      atcctcagg agagggagc aactccagc accgactac 
      tccgaaacg taccacacc accacccga aacgacgac 
      cccctcaga cccagcgtc accaacacg gtgaagagc 
      tacagcaag aagacaatc ccggcgaaa aacgggcga 
      agcatggag acgatcgag tcaagcgag acgaagtcg 
      gtcaccaaa agccactat cggggggat cccagcgtg 
      aagttctac gagaccgag cggagatac agcggggga 
      agccccgta gtcatcgaa gtccgaaat agcaactat 
      aggaaatga ttaactcga agatactgc catataaac 
      tgtcattat ttataattg ttattacta attgtgatt 
      gttttgtcg ttaaaaagg gttttggca ttgttagga 
      tggtagatt aggtgctaa aattcccta aacttttgt 
      atcatatgt tgtttctaa cgtaataat aaatggaaa 
      gaataatcc tagaggtat agaatgtgc caaaataat 
      ttgtaaata cgagc