Status
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Gene information
General information
TC033983
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006183,TC006182
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4592630..4595677
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033983
    iBeetle screen iB_04082

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033983 RA
      MTTNPFTIK QTDQNRHVV ANRLIKSGE IILTETPFV 
      YGPKPGSPV LCLGCFECV DSKTACSKC SWPVCGPEC 
      EFLSSHKDN ECEIFAKNR VKFNDVDDP YDVCLQYEG 
      ITPLRMLLA KEKDPKRWL EEIGGLKIR KNNVNVVDF 
      VRNVCKMDQ FCDDLIHTV CGILDLNAI EVRAPSGYL 
      IRCLYPKLS QISHNCVTN LFQTVTPED FKITLRASG 
      NISENQELF YNYVYPLWP TLIRRDFLK ENKNLDCCC 
      KRCGDKTEL RTHLSTLKC SKCDNGILL SSDSLSDSC 
      DWNCTHCEF KTNASSVKK VYRIVQSEI EAIQMVSGA 
      EGIEQREAI FRKYRSVFH PKNAYMTIL RVDLTQLYG 
      RAPGYTIHE LPDLLQEHK VELCYQLLE VLDVVEPGL 
      SKLRGITLY ELHAPLISL ARNEYKSGL ITREDFRKK 
      MQDAVGLLE KSVEILKLE PPNSVEGEL AIVAKQSLE 
      NLVQNFDLL IQTAVRFFR SKPKRWSEK VKGLKIRKN 
      IVNVVDFVR IVCKMDQFC DDLIHTVCG ILDLNAIEV 
      RAPSGYLTR CLYPKLSQI SHNCVTNLF QTVTPEDFK 
      ITLRASVNI SENQELFYN YVYPLWPTL IRRDFLKEN 
      KNLDCRCKR CGDKTELRT HLSTLKCSK CDNGILLSS 
      DSLSDSCDW NCTHCEFKT NASSVKKVY RIVQSEIEA 
      IQMVSGAEG IEQREAIFR KYRSVFHPK NAYMTILRV 
      DLTQLYGRA PGYTIHELP DLLQEHKVE LCYQLLEVL 
      DVVEPGLSK LRGITLYEL HAPLISLAR NEYKSGLIT 
      REDFRKKMQ DAVGLLEKS VEILKLEPP NSVEGELAI 
      VAKQSLENL VQNFDLLIQ TA
    CDS sequence
    • >TC033983 RA
      atgacgacc aatccgttt actatcaaa caaaccgat 
      caaaaccgt catgttgtg gcaaatcgt cttatcaaa 
      tccggtgaa ataatcctc accgaaacc ccttttgtt 
      tacggccca aaaccagga tcacccgtt ttgtgtctt 
      ggttgtttc gaatgtgtc gactcaaaa acagcgtgt 
      tcgaagtgc tcttggccg gtttgtggc cccgaatgc 
      gaattcttg tccagtcac aaggacaat gagtgcgaa 
      atttttgcc aagaatcgg gtcaaattt aacgatgtg 
      gacgatccc tacgatgtt tgtttacaa tacgagggg 
      ataacaccc cttcggatg ctcctagcg aaggagaaa 
      gaccccaaa cggtggttg gaagaaatc ggagggttg 
      aaaattcgc aaaaacaac gttaatgtc gtggatttt 
      gtacgaaat gtgtgcaaa atggatcaa ttttgtgac 
      gatttaatt cacaccgtt tgtgggatc cttgacttg 
      aatgcgata gaagtgaga gctccttcg ggttatttg 
      attcgttgt ttgtacccg aaactatca caaatttcg 
      cacaattgt gtcacaaat ctctttcaa actgtgacc 
      cctgaggat tttaaaatc acactcaga gcgtctggt 
      aacatttcc gaaaaccag gaattattc tataactac 
      gtttatcca ctttggccc actttaatc cggcgcgat 
      tttttgaaa gaaaacaaa aatttggac tgttgctgt 
      aaaagatgt ggtgataaa accgagcta aggacacac 
      ttgagcacc ttgaagtgt agcaaatgc gacaacggg 
      atacttttg agcagtgat tctttgagc gattcctgc 
      gactggaat tgtactcac tgtgagttc aaaaccaac 
      gcaagttcg gtgaaaaaa gtctacaga atagtccaa 
      agtgaaata gaagccatt cagatggtt tcaggagct 
      gaaggaatc gagcaacgc gaagctatt ttcaggaaa 
      tatcgctcg gtttttcac ccgaaaaac gcctacatg 
      acgatactc agagtcgac ttgacgcag ttatacgga 
      cgagctcct ggttacacc attcatgaa ttaccggat 
      ttgttacag gagcataaa gtcgagttg tgttatcag 
      ttattggaa gttttggac gtcgtagaa cctgggctt 
      tcgaaactc cggggaatc acactgtat gagcttcac 
      gctccttta atttcactc gctcggaat gaatacaag 
      tcggggtta atcacccga gaagacttt cgcaagaaa 
      atgcaggat gctgttgga cttcttgaa aagtcggtg 
      gagattttg aagttggaa ccgccgaac tcagtcgag 
      ggggaattg gcgattgtt gcgaaacaa tcgctggag 
      aatttggtg caaaatttt gatctactc atacaaact 
      gcagttcga tttttccgt tcaaaaccc aaacggtgg 
      tcggagaaa gtcaagggg ttgaaaatt cgcaaaaac 
      atcgttaat gtagtggat tttgtacga attgtgtgc 
      aaaatggat caattttgt gacgattta attcacacc 
      gtttgtggg atccttgac ttgaatgcg atagaagtg 
      agagctcct tcgggttat ttgactcgt tgtttgtac 
      cctaaatta tcacaaatt tcccacaat tgtgtcaca 
      aatctcttt caaactgtg actcctgag gattttaaa 
      atcacactc agagcgtct gttaacatt tccgaaaac 
      caggaatta ttctataac tacgtttat ccactttgg 
      cccacttta attcggcgc gattttttg aaagaaaac 
      aaaaatttg gactgtcgt tgtaaaaga tgtggtgat 
      aaaaccgag ctaaggaca cacttaagc accttaaag 
      tgtagcaaa tgcgacaac gggatactt ttgagcagt 
      gattctttg agcgattcc tgcgactgg aattgcact 
      cactgtgag ttcaaaacg aatgcaagt tcggtgaaa 
      aaagtctac agaatagtc cagagtgaa atagaagcc 
      attcagatg gtttcagga gctgaagga atcgagcaa 
      cgcgaagct attttcagg aaatatcgt tcggttttt 
      cacccgaaa aacgcctac atgacgata ctcagagtc 
      gacttgacg caattatac ggacgagct cctggttac 
      accattcat gaattaccg gatttatta caggagcat 
      aaagtcgag ttgtgttat cagttattg gaagttttg 
      gacgtggta gaacctggg ctttcgaaa ctccgggga 
      atcacactg tatgagctt cacgctcct ttaatttca 
      ctcgctcgg aatgaatac aagtcgggg ttaatcacc 
      cgagaagat tttcggaag aaaatgcag gatgctgtt 
      ggacttctt gaaaagtcg gtggagatt ttgaagttg 
      gaaccgccg aactcagtc gagggggaa ttggcgatt 
      gttgcgaaa caatcgctg gagaatttg gtgcaaaat 
      tttgatcta ctcatacaa actgcgtga
    mRNA sequence
    • >TC033983 RA
      ttttttaaa taactgcac aaaagtaca caacagtcg 
      gttcaagac atgacgacc aatccgttt actatcaaa 
      caaaccgat caaaaccgt catgttgtg gcaaatcgt 
      cttatcaaa tccggtgaa ataatcctc accgaaacc 
      ccttttgtt tacggccca aaaccagga tcacccgtt 
      ttgtgtctt ggttgtttc gaatgtgtc gactcaaaa 
      acagcgtgt tcgaagtgc tcttggccg gtttgtggc 
      cccgaatgc gaattcttg tccagtcac aaggacaat 
      gagtgcgaa atttttgcc aagaatcgg gtcaaattt 
      aacgatgtg gacgatccc tacgatgtt tgtttacaa 
      tacgagggg ataacaccc cttcggatg ctcctagcg 
      aaggagaaa gaccccaaa cggtggttg gaagaaatc 
      ggagggttg aaaattcgc aaaaacaac gttaatgtc 
      gtggatttt gtacgaaat gtgtgcaaa atggatcaa 
      ttttgtgac gatttaatt cacaccgtt tgtgggatc 
      cttgacttg aatgcgata gaagtgaga gctccttcg 
      ggttatttg attcgttgt ttgtacccg aaactatca 
      caaatttcg cacaattgt gtcacaaat ctctttcaa 
      actgtgacc cctgaggat tttaaaatc acactcaga 
      gcgtctggt aacatttcc gaaaaccag gaattattc 
      tataactac gtttatcca ctttggccc actttaatc 
      cggcgcgat tttttgaaa gaaaacaaa aatttggac 
      tgttgctgt aaaagatgt ggtgataaa accgagcta 
      aggacacac ttgagcacc ttgaagtgt agcaaatgc 
      gacaacggg atacttttg agcagtgat tctttgagc 
      gattcctgc gactggaat tgtactcac tgtgagttc 
      aaaaccaac gcaagttcg gtgaaaaaa gtctacaga 
      atagtccaa agtgaaata gaagccatt cagatggtt 
      tcaggagct gaaggaatc gagcaacgc gaagctatt 
      ttcaggaaa tatcgctcg gtttttcac ccgaaaaac 
      gcctacatg acgatactc agagtcgac ttgacgcag 
      ttatacgga cgagctcct ggttacacc attcatgaa 
      ttaccggat ttgttacag gagcataaa gtcgagttg 
      tgttatcag ttattggaa gttttggac gtcgtagaa 
      cctgggctt tcgaaactc cggggaatc acactgtat 
      gagcttcac gctccttta atttcactc gctcggaat 
      gaatacaag tcggggtta atcacccga gaagacttt 
      cgcaagaaa atgcaggat gctgttgga cttcttgaa 
      aagtcggtg gagattttg aagttggaa ccgccgaac 
      tcagtcgag ggggaattg gcgattgtt gcgaaacaa 
      tcgctggag aatttggtg caaaatttt gatctactc 
      atacaaact gcagttcga tttttccgt tcaaaaccc 
      aaacggtgg tcggagaaa gtcaagggg ttgaaaatt 
      cgcaaaaac atcgttaat gtagtggat tttgtacga 
      attgtgtgc aaaatggat caattttgt gacgattta 
      attcacacc gtttgtggg atccttgac ttgaatgcg 
      atagaagtg agagctcct tcgggttat ttgactcgt 
      tgtttgtac cctaaatta tcacaaatt tcccacaat 
      tgtgtcaca aatctcttt caaactgtg actcctgag 
      gattttaaa atcacactc agagcgtct gttaacatt 
      tccgaaaac caggaatta ttctataac tacgtttat 
      ccactttgg cccacttta attcggcgc gattttttg 
      aaagaaaac aaaaatttg gactgtcgt tgtaaaaga 
      tgtggtgat aaaaccgag ctaaggaca cacttaagc 
      accttaaag tgtagcaaa tgcgacaac gggatactt 
      ttgagcagt gattctttg agcgattcc tgcgactgg 
      aattgcact cactgtgag ttcaaaacg aatgcaagt 
      tcggtgaaa aaagtctac agaatagtc cagagtgaa 
      atagaagcc attcagatg gtttcagga gctgaagga 
      atcgagcaa cgcgaagct attttcagg aaatatcgt 
      tcggttttt cacccgaaa aacgcctac atgacgata 
      ctcagagtc gacttgacg caattatac ggacgagct 
      cctggttac accattcat gaattaccg gatttatta 
      caggagcat aaagtcgag ttgtgttat cagttattg 
      gaagttttg gacgtggta gaacctggg ctttcgaaa 
      ctccgggga atcacactg tatgagctt cacgctcct 
      ttaatttca ctcgctcgg aatgaatac aagtcgggg 
      ttaatcacc cgagaagat tttcggaag aaaatgcag 
      gatgctgtt ggacttctt gaaaagtcg gtggagatt 
      ttgaagttg gaaccgccg aactcagtc gagggggaa 
      ttggcgatt gttgcgaaa caatcgctg gagaatttg 
      gtgcaaaat tttgatcta ctcatacaa actgcgtga 
      tttattgta acactgtag taataaaaa ctatttttt 
      ctt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY