Status
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Gene information
General information
TC006174 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4274338..4282294
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006174
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04081

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006174 RA
      MLAKSLLVF ATLVLIIDV RNASKLPLF PKTRNYSDE 
      SISPCSNFY AYACNTWTK QAKKPTFEP VWNPWMAVN 
      HKIKRRLSQ ILTRHDSFL TQPQFFYRS CLNTKDHLN 
      ELKTFIDNL GGWSLIANK RDDNEWFQQ LAVVTRILG 
      IHPILKIHV DLDYKDPKS YAIYVEPGN LIFPEYILT 
      GFEYKTELE AYEKWIYET VNHLSGGKK RINKDQIRE 
      IIKFEMYLA TARNNDARN ERTTVEKLS QKFSIDWIK 
      FLDYIFGSF SIIAPNTTV IVKNYNYLK KLFLLVSQV 
      GNRVARNYL MWSVIKDLS RDTDRYLRN LNFLIDQAI 
      LGVQEDLSR EFECLDKVI AHFSPLLVP KYLKLFVNP 
      RIFPDVKSM VESIKSEFV QILLQCDWL SNEGKSVLV 
      EKIKNIKLH LGYPSWNFH ALKRHYGKV KMTDNHFIN 
      TLALKQFKT SHSLARLAE QKPQTLWPS SPFDVNAYY 
      SVLQNSIFI PLGMLEAPF YQYKREEIS NFGALGSLI 
      GHEISHSLD PAGILADPS GKIRKWLPD RDLKLYKNR 
      ISCFNGSHT IGETIADSN GLKISINAF KKRSPGRKN 
      GQFFISFAQ VWCEISEDT DIFTGDEHA PVSQRIQHI 
      LSNEDFQKT FNCGAKTSC DLW
    CDS sequence
    • >TC006174 RA
      atgctggcg aaaagtctg ctggttttt gccacactt 
      gtgctaatt attgacgtg cggaacgcc agcaagttg 
      ccgctcttt cccaaaacg cggaattat tcagatgag 
      tcaatttcc ccctgctcc aacttttac gcctacgcc 
      tgcaacact tggaccaaa caggccaaa aaaccgaca 
      tttgaaccc gtatggaac ccctggatg gccgttaat 
      cacaaaatc aaacgtcga ttatcccaa attttaact 
      cgtcacgac tccttctta acccaacca caatttttt 
      taccgctcc tgccttaac actaaagac cacttgaac 
      gaattaaaa accttcatt gataattta ggtggttgg 
      tcgttaatt gcgaataaa cgggacgac aatgagtgg 
      ttccaacaa ctggctgtg gttactcga attttgggg 
      attcaccca attttgaaa atccacgtc gatttggac 
      tacaaggac ccgaaaagt tatgccatt tacgtcgaa 
      ccggggaat ttgattttc cccgagtat attttgacc 
      ggttttgag tacaaaacc gaactggaa gcgtacgaa 
      aagtggatc tatgagacc gtgaatcat ttatctggg 
      ggcaaaaaa cgcataaat aaggaccaa attagagaa 
      atcatcaaa tttgaaatg tacttagcg actgcgcga 
      aacaacgac gcgagaaat gaacgcaca actgttgaa 
      aaattgtcg cagaaattt tcaattgat tggatcaaa 
      tttctcgac tacattttc gggagcttt tcaattata 
      gccccgaat acgactgtg atagtcaag aattacaat 
      tatttgaaa aaactgttc cttctagtc agtcaagtg 
      gggaacagg gttgcacgg aattatttg atgtggtcg 
      gtcattaag gacttatcc cgagacact gatcgttat 
      ttgagaaac ttaaatttt ttgatcgac caagcgatt 
      ctaggggtc caggaagat ttgtcgcgt gaatttgaa 
      tgtctggac aaagttatt gcacacttt agccctttg 
      ttggtcccc aaatattta aaattattc gtcaatccc 
      cgaattttc cccgatgtt aaatcaatg gttgaatca 
      atcaaaagc gaattcgtc cagattttg ttgcaatgt 
      gattggttg agcaacgaa gggaaatcg gtacttgtt 
      gagaaaatt aaaaatatt aagttgcac ttggggtac 
      ccttcttgg aatttccac gctcttaaa agacattac 
      ggcaaagta aaaatgacc gataatcat ttcataaat 
      acgcttgca ttgaaacaa ttcaaaaca tcgcacagt 
      ttggcccgt ttagctgag caaaaacca caaacactg 
      tggccctca agcccgttc gacgtgaac gcctattac 
      agcgtcctc caaaattca atttttatt cctttagga 
      atgttagaa gcgccgttc taccaatac aaaagagag 
      gaaatttcg aattttggg gctttaggt tcgctcatt 
      ggccacgaa atttcgcac tctttggat ccagcaggt 
      attttagct gacccaagt ggaaaaatc aggaagtgg 
      ttgccagat cgggactta aagctttac aaaaatcga 
      atttcgtgc tttaatggt agtcacaca attggcgaa 
      acaatcgcc gattcaaac gggttaaaa attagcata 
      aatgcgttc aaaaaacga agtcctggg cggaaaaat 
      gggcagttt tttatcagc tttgctcag gtttggtgc 
      gagatttcg gaagacact gacattttc acaggagac 
      gaacacgcc cctgtttcc cagcgaatc caacacatt 
      ttatccaac gaagacttt caaaaaacg ttcaattgt 
      ggcgcgaaa acttcatgt gacttgtgg tag
    mRNA sequence
    • >TC006174 RA
      tagttgagt gaaatgctg gcgaaaagt ctgctggtt 
      tttgccaca cttgtgcta attattgac gtgcggaac 
      gccagcaag ttgccgctc tttcccaaa acgcggaat 
      tattcagat gagtcaatt tccccctgc tccaacttt 
      tacgcctac gcctgcaac acttggacc aaacaggcc 
      aaaaaaccg acatttgaa cccgtatgg aacccctgg 
      atggccgtt aatcacaaa atcaaacgt cgattatcc 
      caaatttta actcgtcac gactccttc ttaacccaa 
      ccacaattt ttttaccgc tcctgcctt aacactaaa 
      gaccacttg aacgaatta aaaaccttc attgataat 
      ttaggtggt tggtcgtta attgcgaat aaacgggac 
      gacaatgag tggttccaa caactggct gtggttact 
      cgaattttg gggattcac ccaattttg aaaatccac 
      gtcgatttg gactacaag gacccgaaa agttatgcc 
      atttacgtc gaaccgggg aatttgatt ttccccgag 
      tatattttg accggtttt gagtacaaa accgaactg 
      gaagcgtac gaaaagtgg atctatgag accgtgaat 
      catttatct gggggcaaa aaacgcata aataaggac 
      caaattaga gaaatcatc aaatttgaa atgtactta 
      gcgactgcg cgaaacaac gacgcgaga aatgaacgc 
      acaactgtt gaaaaattg tcgcagaaa ttttcaatt 
      gattggatc aaatttctc gactacatt ttcgggagc 
      ttttcaatt atagccccg aatacgact gtgatagtc 
      aagaattac aattatttg aaaaaactg ttccttcta 
      gtcagtcaa gtggggaac agggttgca cggaattat 
      ttgatgtgg tcggtcatt aaggactta tcccgagac 
      actgatcgt tatttgaga aacttaaat tttttgatc 
      gaccaagcg attctaggg gtccaggaa gatttgtcg 
      cgtgaattt gaatgtctg gacaaagtt attgcacac 
      tttagccct ttgttggtc cccaaatat ttaaaatta 
      ttcgtcaat ccccgaatt ttccccgat gttaaatca 
      atggttgaa tcaatcaaa agcgaattc gtccagatt 
      ttgttgcaa tgtgattgg ttgagcaac gaagggaaa 
      tcggtactt gttgagaaa attaaaaat attaagttg 
      cacttgggg tacccttct tggaatttc cacgctctt 
      aaaagacat tacggcaaa gtaaaaatg accgataat 
      catttcata aatacgctt gcattgaaa caattcaaa 
      acatcgcac agtttggcc cgtttagct gagcaaaaa 
      ccacaaaca ctgtggccc tcaagcccg ttcgacgtg 
      aacgcctat tacagcgtc ctccaaaat tcaattttt 
      attccttta ggaatgtta gaagcgccg ttctaccaa 
      tacaaaaga gaggaaatt tcgaatttt ggggcttta 
      ggttcgctc attggccac gaaatttcg cactctttg 
      gatccagca ggtatttta gctgaccca agtggaaaa 
      atcaggaag tggttgcca gatcgggac ttaaagctt 
      tacaaaaat cgaatttcg tgctttaat ggtagtcac 
      acaattggc gaaacaatc gccgattca aacgggtta 
      aaaattagc ataaatgcg ttcaaaaaa cgaagtcct 
      gggcggaaa aatgggcag ttttttatc agctttgct 
      caggtttgg tgcgagatt tcggaagac actgacatt 
      ttcacagga gacgaacac gcccctgtt tcccagcga 
      atccaacac attttatcc aacgaagac tttcaaaaa 
      acgttcaat tgtggcgcg aaaacttca tgtgacttg 
      tggtagaaa taaatttta aaagtgaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY