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Gene information
General information
TC006167 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4202813..4204017
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006167
      iBeetle screen iB_04078

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006167 RA
        MATPTISKK DLQKTLSAK KKKKAEVIK NVLAKPYQN 
        YWPVLNSED NFNLREALR KFLPRLKHD KVNIPYKDI 
        KAIPREERK KFRENYPKP PLAPLLVSD SDRKSIVFG 
        INDVSKCLE DRSAACVLV DSDVQPRLL VQHIVDQAV 
        LYNVPVLVA VTMRPFFKV HCGVSGAAV AFKRGVKPD 
        SGLGLIQQT ICNIYENYP VPQNHIHYH RRLDTITIS 
        DSSNNSVCV VSDSEDEEP AKNYHLKRK LRKRAFVPP 
        EPHKVVESV VYIELDTSV EASPVSKIN YQPLTVKRL 
        KGDKNRNKR KMQKFKKK
      CDS sequence
      • >TC006167 RA
        atggcaaca cctaccatt agtaaaaag gacctacag 
        aaaaccttg tcggctaaa aaaaagaag aaagcggaa 
        gttattaaa aacgtttta gcaaagccc taccaaaac 
        tactggcct gtgctcaat tctgaagac aactttaat 
        ttgagagaa gctctccga aaattcctc ccgagactc 
        aaacatgac aaagttaac atcccgtac aaggatatt 
        aaggcaatt ccgagagaa gaacgcaag aaattccga 
        gaaaattac ccgaaaccg cccttggcc cccttgtta 
        gttagtgat tcggatcga aaaagtatc gtttttggc 
        atcaatgac gtgtcaaaa tgtttggaa gaccgttcg 
        gcagcttgt gttttggtg gacagtgat gtgcagccc 
        cgtctccta gtgcaacac attgtggac caagcggtg 
        ttgtacaat gtcccagtt ttggtggct gttacgatg 
        cgcccgttt ttcaaagtt cactgtggg gtaagtggg 
        gctgcggtc gctttcaag cggggggtt aaacccgat 
        tcgggcttg gggctgatt caacaaact atttgcaat 
        atttatgaa aattatcca gtgccccaa aaccacatt 
        cactatcac aggagacta gacactatt acaattagt 
        gattcctcc aataacagt gtttgtgtt gttagtgac 
        tctgaggat gaagaaccc gcgaagaat tatcatttg 
        aaacgaaag ttgcgaaaa cgggctttt gtgccccca 
        gaaccacat aaggttgtg gaaagtgtt gtttatatt 
        gagttagat acgagtgta gaggcgtct cctgttagt 
        aaaattaat tatcagccc ctcactgtt aagaggctg 
        aaaggggat aaaaatagg aataagcgt aaaatgcaa 
        aaatttaaa aagaagtaa
      mRNA sequence
      • >TC006167 RA
        ttagttttt tagaaagct tgttttctt tggaatgcg 
        acttactcc aacataacc taactattg tcctcgcaa 
        tggcaacac ctaccatta gtaaaaagg acctacaga 
        aaaccttgt cggctaaaa aaaagaaga aagcggaag 
        ttattaaaa acgttttag caaagccct accaaaact 
        actggcctg tgctcaatt ctgaagaca actttaatt 
        tgagagaag ctctccgaa aattcctcc cgagactca 
        aacatgaca aagttaaca tcccgtaca aggatatta 
        aggcaattc cgagagaag aacgcaaga aattccgag 
        aaaattacc cgaaaccgc ccttggccc ccttgttag 
        ttagtgatt cggatcgaa aaagtatcg tttttggca 
        tcaatgacg tgtcaaaat gtttggaag accgttcgg 
        cagcttgtg ttttggtgg acagtgatg tgcagcccc 
        gtctcctag tgcaacaca ttgtggacc aagcggtgt 
        tgtacaatg tcccagttt tggtggctg ttacgatgc 
        gcccgtttt tcaaagttc actgtgggg taagtgggg 
        ctgcggtcg ctttcaagc ggggggtta aacccgatt 
        cgggcttgg ggctgattc aacaaacta tttgcaata 
        tttatgaaa attatccag tgccccaaa accacattc 
        actatcaca ggagactag acactatta caattagtg 
        attcctcca ataacagtg tttgtgttg ttagtgact 
        ctgaggatg aagaacccg cgaagaatt atcatttga 
        aacgaaagt tgcgaaaac gggcttttg tgcccccag 
        aaccacata aggttgtgg aaagtgttg tttatattg 
        agttagata cgagtgtag aggcgtctc ctgttagta 
        aaattaatt atcagcccc tcactgtta agaggctga 
        aaggggata aaaatagga ataagcgta aaatgcaaa 
        aatttaaaa agaagtaaa atttatttt tctgtaaga 
        ggttgttct tcttttggt ttcgttcct tgattccac 
        tcctgtact tccccggac ccccatatc gtatacacc 
        acgatttca agcacatag agtcctatg gtgatgtaa 
        aacaccttc ctccatgaa ttaacacta ggctaaaat 
        tggggattt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY