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Gene information
General information
TC006159 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4119898..4126196
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006159
    iBeetle screen iB_04077

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006159 RA
      MSKHSRRKK GKSARRSQS QSTTSSENF EEGFDVTDD 
      GPIFEVKTS KVMGRYMVS RKNLKPGDV ILSEAPLVI 
      GPCTGCKVQ CLGCYKTLE SSFCTKCTC GWPLCSPKC 
      KGLGKRFGH SQVECAVLK ATKSSKFLN YDNFEEMRP 
      NFNAIVPLR CLLLKETDA RSYQHLMTM ETHNEIRRN 
      IPELWQTNQ RTVVDKIRI DWGLREYSQ EEIHSVCGI 
      LEVNSFEIG QQGVNIRGL YPSAFLMSH DCVPNTNHI 
      DEESTFRLT VRASTRIEP GEMITLSYA YTLQSTLKR 
      REHLLENKF FECQCRRCS DPTELGTFT SALICPKCK 
      DGFVLSSNP LDPEADWTC SNYNKCPGY KISAKSMQL 
      LLDKITQEV DNIDCNDVI TMETFLKKY RNVLHPTHY 
      INLSVKLSL SQLYGRING FLIDELSDE VLTRKLEIC 
      EEIIKVFNV IEPGFTRLR GVTLYEMHA PLMVLLTRQ 
      FERHCTKSE LRSKLKKVL KCLEEASVI LGYEPESSP 
      EGMMGVAAK DALVKIRDW EKILGRF
    CDS sequence
    • >TC006159 RA
      atgagtaaa cattcgcga agaaaaaag ggaaaatcc 
      gcaagacgc tcgcagtca caatcgacc acatcgtcc 
      gaaaatttc gaagaaggc ttcgatgtc actgatgat 
      ggcccgatc ttcgaggtc aaaacgtcc aaagtcatg 
      ggcagatac atggtttcg cggaaaaac ctaaaacct 
      ggcgatgtt atcctgagc gaagcaccc cttgttatt 
      ggtccttgc accggctgc aaagtgcaa tgtttgggg 
      tgctacaaa actctcgaa tcgagtttt tgcaccaag 
      tgcacttgt ggttggcca ttgtgttcg cccaaatgt 
      aaaggactc gggaagaga tttggccac agtcaagtc 
      gaatgtgcg gtgctcaaa gccaccaaa tcgtcaaag 
      tttctcaac tatgataat tttgaagaa atgcgcccg 
      aatttcaac gcgatagta cctctaagg tgtcttcta 
      ctcaaagaa acagatgct aggagttac caacacttg 
      atgacgatg gaaactcac aatgagatt cgacgaaac 
      attccagag ttatggcaa accaatcag agaacagtt 
      gtcgacaag attcgcatt gattggggt ttgagggaa 
      tactcacaa gaggaaatt cacagtgtt tgcggcatt 
      ttggaagtc aattcgttc gaaattggg caacaagga 
      gtcaacatc cgagggctg tacccttct gccttccta 
      atgtcacac gattgtgtc cctaacacg aatcacatt 
      gacgaagaa agtaccttc aggttaaca gttcgggcc 
      tcgactagg atagaacct ggcgaaatg ataacttta 
      agttatgcc tacacgttg caaagtacc ctaaaaaga 
      cgggaacac ctactagaa aacaagttc ttcgagtgt 
      cagtgtcga cgttgtagc gaccctacg gaacttggc 
      accttcaca agcgcccta atttgccct aaatgcaag 
      gacgggttc gtactctcg tccaaccct ttggacccc 
      gaagctgac tggacttgt tccaattac aacaaatgt 
      ccaggttat aaaatcagc gcaaagtca atgcaactc 
      ctcctagac aaaataacc caagaagtt gacaacatc 
      gactgtaat gatgttata acaatggaa acttttcta 
      aagaagtac cgaaacgtg ctccacccg actcactac 
      atcaacctc agtgtgaag ctctccctt agccaactc 
      tacggtcgc atcaacggg tttctaatc gatgagctc 
      agcgatgaa gtcctaacc cgaaaactg gagatttgc 
      gaggaaatt atcaaagtt ttcaacgtc atagagccc 
      ggttttacg cgactacgt ggagtcacc ctgtatgag 
      atgcatgcg cctttgatg gtactcctg acgaggcag 
      tttgagcgt cattgcact aagagtgag ctaaggagc 
      aaattgaag aaggttttg aaatgtttg gaagaagcg 
      agtgtgatt ttgggatac gaacccgag agttcgcct 
      gaagggatg atgggggtt gcggccaag gatgcgttg 
      gttaagatt agggattgg gagaagatt ttagggagg 
      ttttag
    mRNA sequence
    • >TC006159 RA
      agttgacta aaataaacg gccactccg ttacaacca 
      aatttcggt gacaagttc tgacaacaa catgagtaa 
      acattcgcg aagaaaaaa gggaaaatc cgcaagacg 
      ctcgcagtc acaatcgac cacatcgtc cgaaaattt 
      cgaagaagg cttcgatgt cactgatga tggcccgat 
      cttcgaggt caaaacgtc caaagtcat gggcagata 
      catggtttc gcggaaaaa cctaaaacc tggcgatgt 
      tatcctgag cgaagcacc ccttgttat tggtccttg 
      caccggctg caaagtgca atgtttggg gtgctacaa 
      aactctcga atcgagttt ttgcaccaa gtgcacttg 
      tggttggcc attgtgttc gcccaaatg taaaggact 
      cgggaagag atttggcca cagtcaagt cgaatgtgc 
      ggtgctcaa agccaccaa atcgtcaaa gtttctcaa 
      ctatgataa ttttgaaga aatgcgccc gaatttcaa 
      cgcgatagt acctctaag gtgtcttct actcaaaga 
      aacagatgc taggagtta ccaacactt gatgacgat 
      ggaaactca caatgagat tcgacgaaa cattccaga 
      gttatggca aaccaatca gagaacagt tgtcgacaa 
      gattcgcat tgattgggg tttgaggga atactcaca 
      agaggaaat tcacagtgt ttgcggcat tttggaagt 
      caattcgtt cgaaattgg gcaacaagg agtcaacat 
      ccgagggct gtacccttc tgccttcct aatgtcaca 
      cgattgtgt ccctaacac gaatcacat tgacgaaga 
      aagtacctt caggttaac agttcgggc ctcgactag 
      gatagaacc tggcgaaat gataacttt aagttatgc 
      ctacacgtt gcaaagtac cctaaaaag acgggaaca 
      cctactaga aaacaagtt cttcgagtg tcagtgtcg 
      acgttgtag cgaccctac ggaacttgg caccttcac 
      aagcgccct aatttgccc taaatgcaa ggacgggtt 
      cgtactctc gtccaaccc tttggaccc cgaagctga 
      ctggacttg ttccaatta caacaaatg tccaggtta 
      taaaatcag cgcaaagtc aatgcaact cctcctaga 
      caaaataac ccaagaagt tgacaacat cgactgtaa 
      tgatgttat aacaatgga aacttttct aaagaagta 
      ccgaaacgt gctccaccc gactcacta catcaacct 
      cagtgtgaa gctctccct tagccaact ctacggtcg 
      catcaacgg gtttctaat cgatgagct cagcgatga 
      agtcctaac ccgaaaact ggagatttg cgaggaaat 
      tatcaaagt tttcaacgt catagagcc cggttttac 
      gcgactacg tggagtcac cctgtatga gatgcatgc 
      gcctttgat ggtactcct gacgaggca gtttgagcg 
      tcattgcac taagagtga gctaaggag caaattgaa 
      gaaggtttt gaaatgttt ggaagaagc gagtgtgat 
      tttgggata cgaacccga gagttcgcc tgaagggat 
      gatgggggt tgcggccaa ggatgcgtt ggttaagat 
      tagggattg ggagaagat tttagggag gttttagta 
      taaataaat agtttttta attg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY