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Gene information
General information
TC033971
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006150,TC006151 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3846725..3874359
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033971
    iBeetle screen iB_04075

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033971 RA
      MSTAAATFL GTVRIKCTC TPLLLENLD RISVLFAAR 
LEEAHSKDE FVDFEVHSL APSGCERAP SAADFITRL 
QKYVDDICG EGCQRSVAG EDSSYDSDY EADPTPSRH 
QDVSRTASD TLAAEYAEY VETTPSDAS PGYTARVEF 
ALKLGYTEK LVQAALQKL GPSPAQNEL LAELIKLGA 
QKGGSCDSS PSESTLDVT DVQGEQAGG QALRPIVID 
GSNVAMSHG NKEIFSCRG IKICVDWFK SRGHKDITV 
FVPKWRKEA PRPDNPVRD QDILAELEK ERLLVFTPS 
RLVGGKRMV CYDDRYILR LAAEVDGIV VSNDNYRDL 
CQESAEFRK VVEERILMY SFVNDRFMP PDDPLGRSG 
PTLDNFLRT QPKKGDPPP PCPYAKKCT YGNKCKYHH 
PERGPFPHK SVTERLSEH AQRHLQARE GDIRKTPLG 
RTRSNVPPP KEVQHVVVS KSRSVDNVG AAPPQPENL 
HRKLQRQLT LNPASDPRL VRRYVAPQP PYMNWDMHQ 
HVTRIASAP DSYRPWPPA APTHRHMQR MSSCSDPQL 
NVWPPLWSQ GGPPPPPTL QEDARRKLH YHLAAIFPE 
EQVTQAMRM YPDETNPQK ICAAILSMF PKT
    CDS sequence
    • >TC033971 RA
      atgtcaacg gccgcagcg acatttcta ggcacagtg 
agaataaaa tgcacttgt acgcccctt ctcttggag 
aatttggac cgaatttcg gtgctgttc gcggcacga 
cttgaagaa gcccacagc aaagacgag tttgtcgat 
ttcgaagtg cattcgttg gcccccagc ggatgcgaa 
cgcgccccc agcgccgcc gacttcata accaggttg 
cagaaatat gtagacgac atttgcggc gagggatgt 
cagcgttcc gtcgccggc gaagactcc agctacgat 
tcggactac gaagccgat ccgacgcct agccgccac 
caggatgtc tcccggacc gccagcgac acgcttgcc 
gccgaatac gccgagtac gtcgaaacg acgccgtcg 
gacgcctca ccaggttac acggcgcgc gtcgagttt 
gcgctgaaa ctcggctac accgagaag ctggtgcag 
gcggcgcta cagaaattg gggccgtcg ccggcgcag 
aacgaactg ctggcggag ttgattaag ttgggggcg 
cagaaaggg ggctcctgt gatagcagt ccgtcggag 
agtacgctg gacgtgacc gatgtccaa ggggaacag 
gccggaggg caggctctg aggccgatt gttatcgat 
gggagcaat gttgcgatg agccacggc aacaaggag 
atattctca tgccgcggc ataaaaatc tgcgtggat 
tggttcaaa tcacgcggc cacaaagac atcaccgtc 
ttcgtgccg aaatggcgc aaagaagcc ccccgtccc 
gacaacccc gtccgcgac caagacatt ctagccgaa 
ctggaaaaa gaaagactg ctcgtattc accccatcg 
cgtctcgtc ggcggcaaa cgcatggtc tgctacgac 
gaccgctac atcctccgg ctggcagcc gaagtggac 
ggcatcgtc gtcagcaac gacaactac cgcgacctc 
tgccaagaa agcgccgag tttcgcaaa gtggtcgaa 
gagcgcatt ctcatgtac agcttcgtc aacgaccgc 
ttcatgccc cccgacgac cccctgggg cgctccggc 
cccacccta gacaacttc ctccgaacg cagcccaaa 
aaaggggac cccccgccc ccttgtccc tacgccaaa 
aagtgcaca tacggcaac aaatgcaag taccaccac 
cccgaacgc ggccccttc ccgcataaa agcgtaacc 
gaacggctg tcagaacac gcacaaaga cacctccaa 
gcgcgcgaa ggcgacatc cggaaaacc cccttgggg 
cgcacccgc tccaacgtg cccccaccc aaagaagtg 
caacatgtc gtggtgtcg aaaagccgc agtgtggat 
aatgtgggg gctgcgccc ccccaacct gagaacctc 
caccggaag ctccaaaga caactgaca ctaaacccg 
gcgtctgat ccgagattg gtgcggcgg tacgtggcc 
ccacagccc ccttacatg aactgggac atgcaccag 
cacgtgacg cggattgcg tcagctccg gattcgtac 
aggccgtgg cccccggca gcccccacg catcgacat 
atgcaaaga atgtcgagt tgttccgat ccgcagttg 
aatgtttgg ccgccgttg tggtcgcaa gggggccca 
ccaccgccc cctacactg caggaggat gcgcgcagg 
aaattgcac tatcatcta gcggcgatt tttcccgaa 
gaacaagtc acacaagcg atgaggatg tatccggat 
gagacgaat ccgcagaag atttgcgcg gcgatttta 
tcaatgttt cccaaaacc taa
    mRNA sequence
    • >TC033971 RA
      gatgtcgtc tgtcagctg tccgcgagc cgtcaatcg 
aaataaata aagtgcgtt cgtgtctca acccgtaat 
tattaatag aaataaagt ggatgaaaa gttcgtgcg 
cgttcagca tgtaacaag catgggctt tggcgaaaa 
tgtcaacgg ccgcagcga catttctag gcacagtga 
gaataaaat gcacttgta cgccccttc tcttggaga 
atttggacc gaatttcgg tgctgttcg cggcacgac 
ttgaagaag cccacagca aagacgagt ttgtcgatt 
tcgaagtgc attcgttgg cccccagcg gatgcgaac 
gcgccccca gcgccgccg acttcataa ccaggttgc 
agaaatatg tagacgaca tttgcggcg agggatgtc 
agcgttccg tcgccggcg aagactcca gctacgatt 
cggactacg aagccgatc cgacgccta gccgccacc 
aggatgtct cccggaccg ccagcgaca cgcttgccg 
ccgaatacg ccgagtacg tcgaaacga cgccgtcgg 
acgcctcac caggttaca cggcgcgcg tcgagtttg 
cgctgaaac tcggctaca ccgagaagc tggtgcagg 
cggcgctac agaaattgg ggccgtcgc cggcgcaga 
acgaactgc tggcggagt tgattaagt tgggggcgc 
agaaagggg gctcctgtg atagcagtc cgtcggaga 
gtacgctgg acgtgaccg atgtccaag gggaacagg 
ccggagggc aggctctga ggccgattg ttatcgatg 
ggagcaatg ttgcgatga gccacggca acaaggaga 
tattctcat gccgcggca taaaaatct gcgtggatt 
ggttcaaat cacgcggcc acaaagaca tcaccgtct 
tcgtgccga aatggcgca aagaagccc cccgtcccg 
acaaccccg tccgcgacc aagacattc tagccgaac 
tggaaaaag aaagactgc tcgtattca ccccatcgc 
gtctcgtcg gcggcaaac gcatggtct gctacgacg 
accgctaca tcctccggc tggcagccg aagtggacg 
gcatcgtcg tcagcaacg acaactacc gcgacctct 
gccaagaaa gcgccgagt ttcgcaaag tggtcgaag 
agcgcattc tcatgtaca gcttcgtca acgaccgct 
tcatgcccc ccgacgacc ccctggggc gctccggcc 
ccaccctag acaacttcc tccgaacgc agcccaaaa 
aaggggacc ccccgcccc cttgtccct acgccaaaa 
agtgcacat acggcaaca aatgcaagt accaccacc 
ccgaacgcg gccccttcc cgcataaaa gcgtaaccg 
aacggctgt cagaacacg cacaaagac acctccaag 
cgcgcgaag gcgacatcc ggaaaaccc ccttggggc 
gcacccgct ccaacgtgc ccccaccca aagaagtgc 
aacatgtcg tggtgtcga aaagccgca gtgtggata 
atgtggggg ctgcgcccc cccaacctg agaacctcc 
accggaagc tccaaagac aactgacac taaacccgg 
cgtctgatc cgagattgg tgcggcggt acgtggccc 
cacagcccc cttacatga actgggaca tgcaccagc 
acgtgacgc ggattgcgt cagctccgg attcgtaca 
ggccgtggc ccccggcag cccccacgc atcgacata 
tgcaaagaa tgtcgagtt gttccgatc cgcagttga 
atgtttggc cgccgttgt ggtcgcaag ggggcccac 
caccgcccc ctacactgc aggaggatg cgcgcagga 
aattgcact atcatctag cggcgattt ttcccgaag 
aacaagtca cacaagcga tgaggatgt atccggatg 
agacgaatc cgcagaaga tttgcgcgg cgattttat 
caatgtttc ccaaaacct aattggata cttctatgg 
gcttatgtg agattaagc gtatacgag gtttggtca 
gttattatc taaacgcaa tggatttac gatgaggtg 
tagtttaga ttgtccaaa ataacttca tattgaaga 
attcagagt aatgataga gaaactaag taggttatt 
actaagata cgagattct gttggagac agaggaact 
tttatctac gaggagaca aaataacac gactttctg 
tcctcaaaa atttgtggg gaataacgt tttgaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY