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Gene information
General information
TC033971
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006150,TC006151
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3541303..3568937
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033971
    iBeetle screen iB_04075
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033971 RA
      MSTAAATFL GTVRIKCTC TPLLLENLD RISVLFAAR 
      LEEAHSKDE FVDFEVHSL APSGCERAP SAADFITRL 
      QKYVDDICG EGCQRSVAG EDSSYDSDY EADPTPSRH 
      QDVSRTASD TLAAEYAEY VETTPSDAS PGYTARVEF 
      ALKLGYTEK LVQAALQKL GPSPAQNEL LAELIKLGA 
      QKGGSCDSS PSESTLDVT DVQGEQAGG QALRPIVID 
      GSNVAMSHG NKEIFSCRG IKICVDWFK SRGHKDITV 
      FVPKWRKEA PRPDNPVRD QDILAELEK ERLLVFTPS 
      RLVGGKRMV CYDDRYILR LAAEVDGIV VSNDNYRDL 
      CQESAEFRK VVEERILMY SFVNDRFMP PDDPLGRSG 
      PTLDNFLRT QPKKGDPPP PCPYAKKCT YGNKCKYHH 
      PERGPFPHK SVTERLSEH AQRHLQARE GDIRKTPLG 
      RTRSNVPPP KEVQHVVVS KSRSVDNVG AAPPQPENL 
      HRKLQRQLT LNPASDPRL VRRYVAPQP PYMNWDMHQ 
      HVTRIASAP DSYRPWPPA APTHRHMQR MSSCSDPQL 
      NVWPPLWSQ GGPPPPPTL QEDARRKLH YHLAAIFPE 
      EQVTQAMRM YPDETNPQK ICAAILSMF PKT
    CDS sequence
    • >TC033971 RA
      atgtcaacg gccgcagcg acatttcta ggcacagtg 
      agaataaaa tgcacttgt acgcccctt ctcttggag 
      aatttggac cgaatttcg gtgctgttc gcggcacga 
      cttgaagaa gcccacagc aaagacgag tttgtcgat 
      ttcgaagtg cattcgttg gcccccagc ggatgcgaa 
      cgcgccccc agcgccgcc gacttcata accaggttg 
      cagaaatat gtagacgac atttgcggc gagggatgt 
      cagcgttcc gtcgccggc gaagactcc agctacgat 
      tcggactac gaagccgat ccgacgcct agccgccac 
      caggatgtc tcccggacc gccagcgac acgcttgcc 
      gccgaatac gccgagtac gtcgaaacg acgccgtcg 
      gacgcctca ccaggttac acggcgcgc gtcgagttt 
      gcgctgaaa ctcggctac accgagaag ctggtgcag 
      gcggcgcta cagaaattg gggccgtcg ccggcgcag 
      aacgaactg ctggcggag ttgattaag ttgggggcg 
      cagaaaggg ggctcctgt gatagcagt ccgtcggag 
      agtacgctg gacgtgacc gatgtccaa ggggaacag 
      gccggaggg caggctctg aggccgatt gttatcgat 
      gggagcaat gttgcgatg agccacggc aacaaggag 
      atattctca tgccgcggc ataaaaatc tgcgtggat 
      tggttcaaa tcacgcggc cacaaagac atcaccgtc 
      ttcgtgccg aaatggcgc aaagaagcc ccccgtccc 
      gacaacccc gtccgcgac caagacatt ctagccgaa 
      ctggaaaaa gaaagactg ctcgtattc accccatcg 
      cgtctcgtc ggcggcaaa cgcatggtc tgctacgac 
      gaccgctac atcctccgg ctggcagcc gaagtggac 
      ggcatcgtc gtcagcaac gacaactac cgcgacctc 
      tgccaagaa agcgccgag tttcgcaaa gtggtcgaa 
      gagcgcatt ctcatgtac agcttcgtc aacgaccgc 
      ttcatgccc cccgacgac cccctgggg cgctccggc 
      cccacccta gacaacttc ctccgaacg cagcccaaa 
      aaaggggac cccccgccc ccttgtccc tacgccaaa 
      aagtgcaca tacggcaac aaatgcaag taccaccac 
      cccgaacgc ggccccttc ccgcataaa agcgtaacc 
      gaacggctg tcagaacac gcacaaaga cacctccaa 
      gcgcgcgaa ggcgacatc cggaaaacc cccttgggg 
      cgcacccgc tccaacgtg cccccaccc aaagaagtg 
      caacatgtc gtggtgtcg aaaagccgc agtgtggat 
      aatgtgggg gctgcgccc ccccaacct gagaacctc 
      caccggaag ctccaaaga caactgaca ctaaacccg 
      gcgtctgat ccgagattg gtgcggcgg tacgtggcc 
      ccacagccc ccttacatg aactgggac atgcaccag 
      cacgtgacg cggattgcg tcagctccg gattcgtac 
      aggccgtgg cccccggca gcccccacg catcgacat 
      atgcaaaga atgtcgagt tgttccgat ccgcagttg 
      aatgtttgg ccgccgttg tggtcgcaa gggggccca 
      ccaccgccc cctacactg caggaggat gcgcgcagg 
      aaattgcac tatcatcta gcggcgatt tttcccgaa 
      gaacaagtc acacaagcg atgaggatg tatccggat 
      gagacgaat ccgcagaag atttgcgcg gcgatttta 
      tcaatgttt cccaaaacc taa
    mRNA sequence
    • >TC033971 RA
      gatgtcgtc tgtcagctg tccgcgagc cgtcaatcg 
      aaataaata aagtgcgtt cgtgtctca acccgtaat 
      tattaatag aaataaagt ggatgaaaa gttcgtgcg 
      cgttcagca tgtaacaag catgggctt tggcgaaaa 
      tgtcaacgg ccgcagcga catttctag gcacagtga 
      gaataaaat gcacttgta cgccccttc tcttggaga 
      atttggacc gaatttcgg tgctgttcg cggcacgac 
      ttgaagaag cccacagca aagacgagt ttgtcgatt 
      tcgaagtgc attcgttgg cccccagcg gatgcgaac 
      gcgccccca gcgccgccg acttcataa ccaggttgc 
      agaaatatg tagacgaca tttgcggcg agggatgtc 
      agcgttccg tcgccggcg aagactcca gctacgatt 
      cggactacg aagccgatc cgacgccta gccgccacc 
      aggatgtct cccggaccg ccagcgaca cgcttgccg 
      ccgaatacg ccgagtacg tcgaaacga cgccgtcgg 
      acgcctcac caggttaca cggcgcgcg tcgagtttg 
      cgctgaaac tcggctaca ccgagaagc tggtgcagg 
      cggcgctac agaaattgg ggccgtcgc cggcgcaga 
      acgaactgc tggcggagt tgattaagt tgggggcgc 
      agaaagggg gctcctgtg atagcagtc cgtcggaga 
      gtacgctgg acgtgaccg atgtccaag gggaacagg 
      ccggagggc aggctctga ggccgattg ttatcgatg 
      ggagcaatg ttgcgatga gccacggca acaaggaga 
      tattctcat gccgcggca taaaaatct gcgtggatt 
      ggttcaaat cacgcggcc acaaagaca tcaccgtct 
      tcgtgccga aatggcgca aagaagccc cccgtcccg 
      acaaccccg tccgcgacc aagacattc tagccgaac 
      tggaaaaag aaagactgc tcgtattca ccccatcgc 
      gtctcgtcg gcggcaaac gcatggtct gctacgacg 
      accgctaca tcctccggc tggcagccg aagtggacg 
      gcatcgtcg tcagcaacg acaactacc gcgacctct 
      gccaagaaa gcgccgagt ttcgcaaag tggtcgaag 
      agcgcattc tcatgtaca gcttcgtca acgaccgct 
      tcatgcccc ccgacgacc ccctggggc gctccggcc 
      ccaccctag acaacttcc tccgaacgc agcccaaaa 
      aaggggacc ccccgcccc cttgtccct acgccaaaa 
      agtgcacat acggcaaca aatgcaagt accaccacc 
      ccgaacgcg gccccttcc cgcataaaa gcgtaaccg 
      aacggctgt cagaacacg cacaaagac acctccaag 
      cgcgcgaag gcgacatcc ggaaaaccc ccttggggc 
      gcacccgct ccaacgtgc ccccaccca aagaagtgc 
      aacatgtcg tggtgtcga aaagccgca gtgtggata 
      atgtggggg ctgcgcccc cccaacctg agaacctcc 
      accggaagc tccaaagac aactgacac taaacccgg 
      cgtctgatc cgagattgg tgcggcggt acgtggccc 
      cacagcccc cttacatga actgggaca tgcaccagc 
      acgtgacgc ggattgcgt cagctccgg attcgtaca 
      ggccgtggc ccccggcag cccccacgc atcgacata 
      tgcaaagaa tgtcgagtt gttccgatc cgcagttga 
      atgtttggc cgccgttgt ggtcgcaag ggggcccac 
      caccgcccc ctacactgc aggaggatg cgcgcagga 
      aattgcact atcatctag cggcgattt ttcccgaag 
      aacaagtca cacaagcga tgaggatgt atccggatg 
      agacgaatc cgcagaaga tttgcgcgg cgattttat 
      caatgtttc ccaaaacct aattggata cttctatgg 
      gcttatgtg agattaagc gtatacgag gtttggtca 
      gttattatc taaacgcaa tggatttac gatgaggtg 
      tagtttaga ttgtccaaa ataacttca tattgaaga 
      attcagagt aatgataga gaaactaag taggttatt 
      actaagata cgagattct gttggagac agaggaact 
      tttatctac gaggagaca aaataacac gactttctg 
      tcctcaaaa atttgtggg gaataacgt tttgaatt