| General information | |||
| TC033971
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 3846725..3874359 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033971 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04075 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)
- >TC033971 RA
MSTAAATFL GTVRIKCTC TPLLLENLD RISVLFAAR LEEAHSKDE FVDFEVHSL APSGCERAP SAADFITRL QKYVDDICG EGCQRSVAG EDSSYDSDY EADPTPSRH QDVSRTASD TLAAEYAEY VETTPSDAS PGYTARVEF ALKLGYTEK LVQAALQKL GPSPAQNEL LAELIKLGA QKGGSCDSS PSESTLDVT DVQGEQAGG QALRPIVID GSNVAMSHG NKEIFSCRG IKICVDWFK SRGHKDITV FVPKWRKEA PRPDNPVRD QDILAELEK ERLLVFTPS RLVGGKRMV CYDDRYILR LAAEVDGIV VSNDNYRDL CQESAEFRK VVEERILMY SFVNDRFMP PDDPLGRSG PTLDNFLRT QPKKGDPPP PCPYAKKCT YGNKCKYHH PERGPFPHK SVTERLSEH AQRHLQARE GDIRKTPLG RTRSNVPPP KEVQHVVVS KSRSVDNVG AAPPQPENL HRKLQRQLT LNPASDPRL VRRYVAPQP PYMNWDMHQ HVTRIASAP DSYRPWPPA APTHRHMQR MSSCSDPQL NVWPPLWSQ GGPPPPPTL QEDARRKLH YHLAAIFPE EQVTQAMRM YPDETNPQK ICAAILSMF PKT
- >TC033971 RA
atgtcaacg gccgcagcg acatttcta ggcacagtg agaataaaa tgcacttgt acgcccctt ctcttggag aatttggac cgaatttcg gtgctgttc gcggcacga cttgaagaa gcccacagc aaagacgag tttgtcgat ttcgaagtg cattcgttg gcccccagc ggatgcgaa cgcgccccc agcgccgcc gacttcata accaggttg cagaaatat gtagacgac atttgcggc gagggatgt cagcgttcc gtcgccggc gaagactcc agctacgat tcggactac gaagccgat ccgacgcct agccgccac caggatgtc tcccggacc gccagcgac acgcttgcc gccgaatac gccgagtac gtcgaaacg acgccgtcg gacgcctca ccaggttac acggcgcgc gtcgagttt gcgctgaaa ctcggctac accgagaag ctggtgcag gcggcgcta cagaaattg gggccgtcg ccggcgcag aacgaactg ctggcggag ttgattaag ttgggggcg cagaaaggg ggctcctgt gatagcagt ccgtcggag agtacgctg gacgtgacc gatgtccaa ggggaacag gccggaggg caggctctg aggccgatt gttatcgat gggagcaat gttgcgatg agccacggc aacaaggag atattctca tgccgcggc ataaaaatc tgcgtggat tggttcaaa tcacgcggc cacaaagac atcaccgtc ttcgtgccg aaatggcgc aaagaagcc ccccgtccc gacaacccc gtccgcgac caagacatt ctagccgaa ctggaaaaa gaaagactg ctcgtattc accccatcg cgtctcgtc ggcggcaaa cgcatggtc tgctacgac gaccgctac atcctccgg ctggcagcc gaagtggac ggcatcgtc gtcagcaac gacaactac cgcgacctc tgccaagaa agcgccgag tttcgcaaa gtggtcgaa gagcgcatt ctcatgtac agcttcgtc aacgaccgc ttcatgccc cccgacgac cccctgggg cgctccggc cccacccta gacaacttc ctccgaacg cagcccaaa aaaggggac cccccgccc ccttgtccc tacgccaaa aagtgcaca tacggcaac aaatgcaag taccaccac cccgaacgc ggccccttc ccgcataaa agcgtaacc gaacggctg tcagaacac gcacaaaga cacctccaa gcgcgcgaa ggcgacatc cggaaaacc cccttgggg cgcacccgc tccaacgtg cccccaccc aaagaagtg caacatgtc gtggtgtcg aaaagccgc agtgtggat aatgtgggg gctgcgccc ccccaacct gagaacctc caccggaag ctccaaaga caactgaca ctaaacccg gcgtctgat ccgagattg gtgcggcgg tacgtggcc ccacagccc ccttacatg aactgggac atgcaccag cacgtgacg cggattgcg tcagctccg gattcgtac aggccgtgg cccccggca gcccccacg catcgacat atgcaaaga atgtcgagt tgttccgat ccgcagttg aatgtttgg ccgccgttg tggtcgcaa gggggccca ccaccgccc cctacactg caggaggat gcgcgcagg aaattgcac tatcatcta gcggcgatt tttcccgaa gaacaagtc acacaagcg atgaggatg tatccggat gagacgaat ccgcagaag atttgcgcg gcgatttta tcaatgttt cccaaaacc taa
- >TC033971 RA
gatgtcgtc tgtcagctg tccgcgagc cgtcaatcg aaataaata aagtgcgtt cgtgtctca acccgtaat tattaatag aaataaagt ggatgaaaa gttcgtgcg cgttcagca tgtaacaag catgggctt tggcgaaaa tgtcaacgg ccgcagcga catttctag gcacagtga gaataaaat gcacttgta cgccccttc tcttggaga atttggacc gaatttcgg tgctgttcg cggcacgac ttgaagaag cccacagca aagacgagt ttgtcgatt tcgaagtgc attcgttgg cccccagcg gatgcgaac gcgccccca gcgccgccg acttcataa ccaggttgc agaaatatg tagacgaca tttgcggcg agggatgtc agcgttccg tcgccggcg aagactcca gctacgatt cggactacg aagccgatc cgacgccta gccgccacc aggatgtct cccggaccg ccagcgaca cgcttgccg ccgaatacg ccgagtacg tcgaaacga cgccgtcgg acgcctcac caggttaca cggcgcgcg tcgagtttg cgctgaaac tcggctaca ccgagaagc tggtgcagg cggcgctac agaaattgg ggccgtcgc cggcgcaga acgaactgc tggcggagt tgattaagt tgggggcgc agaaagggg gctcctgtg atagcagtc cgtcggaga gtacgctgg acgtgaccg atgtccaag gggaacagg ccggagggc aggctctga ggccgattg ttatcgatg ggagcaatg ttgcgatga gccacggca acaaggaga tattctcat gccgcggca taaaaatct gcgtggatt ggttcaaat cacgcggcc acaaagaca tcaccgtct tcgtgccga aatggcgca aagaagccc cccgtcccg acaaccccg tccgcgacc aagacattc tagccgaac tggaaaaag aaagactgc tcgtattca ccccatcgc gtctcgtcg gcggcaaac gcatggtct gctacgacg accgctaca tcctccggc tggcagccg aagtggacg gcatcgtcg tcagcaacg acaactacc gcgacctct gccaagaaa gcgccgagt ttcgcaaag tggtcgaag agcgcattc tcatgtaca gcttcgtca acgaccgct tcatgcccc ccgacgacc ccctggggc gctccggcc ccaccctag acaacttcc tccgaacgc agcccaaaa aaggggacc ccccgcccc cttgtccct acgccaaaa agtgcacat acggcaaca aatgcaagt accaccacc ccgaacgcg gccccttcc cgcataaaa gcgtaaccg aacggctgt cagaacacg cacaaagac acctccaag cgcgcgaag gcgacatcc ggaaaaccc ccttggggc gcacccgct ccaacgtgc ccccaccca aagaagtgc aacatgtcg tggtgtcga aaagccgca gtgtggata atgtggggg ctgcgcccc cccaacctg agaacctcc accggaagc tccaaagac aactgacac taaacccgg cgtctgatc cgagattgg tgcggcggt acgtggccc cacagcccc cttacatga actgggaca tgcaccagc acgtgacgc ggattgcgt cagctccgg attcgtaca ggccgtggc ccccggcag cccccacgc atcgacata tgcaaagaa tgtcgagtt gttccgatc cgcagttga atgtttggc cgccgttgt ggtcgcaag ggggcccac caccgcccc ctacactgc aggaggatg cgcgcagga aattgcact atcatctag cggcgattt ttcccgaag aacaagtca cacaagcga tgaggatgt atccggatg agacgaatc cgcagaaga tttgcgcgg cgattttat caatgtttc ccaaaacct aattggata cttctatgg gcttatgtg agattaagc gtatacgag gtttggtca gttattatc taaacgcaa tggatttac gatgaggtg tagtttaga ttgtccaaa ataacttca tattgaaga attcagagt aatgataga gaaactaag taggttatt actaagata cgagattct gttggagac agaggaact tttatctac gaggagaca aaataacac gactttctg tcctcaaaa atttgtggg gaataacgt tttgaatt
