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Gene information
General information
TC006146 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3725566..3727490
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006146
    iBeetle screen iB_04074

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006146 RA
      MTTHSHKVR RVQGHECSL PQEDELITL YINGGLFVD 
      FRRRFRQYL MVSAANPES KTVLKSTAQ IRLEESRHL 
      RRYYHMIHP FSEARMTWE MIMIVVYTS LLVLMPLEL 
      GIPITESLI LYSKFFLDL ISLMDIALF FCTGYFDVR 
      KHKIILKPT LVLKNYLFP FFFFDLISS LPYNMYIVQ 
      TKSYDLRWL KFFYLVKLT RLPTLSKYI RKFCIRMEL 
      YSYKLSTVN FALWFFTAI WWATAITLL VYSSVENYS 
      LQYYIENPD IFNPTLESC YQVVRAFML VAMSKIQTD 
      RPLTMIFNI ICILIGSVL NMVILAQVM QIYKRHSSS 
      RNKYDNLLQ EIAEYMNYK ELPPPIKNR VFRYVEFKF 
      QKNIFKEAD ILNTLSKIL KQDILLHNC KKMVEKVDF 
      FKGLPGSLI LRLVTKLRS EIYLPNDLI VQAGIPGNA 
      MYFIYVGSV AVYTINEQE ICHLEDGSY FGEISLVIN 
      EPRVASVIA ITNCEVFRL SRKDFMEAM EPYPNLTGK 
      IREKALSRL KRTKQMTDD YHQRIKAKV SVAFDEPTI 
      LD
    CDS sequence
    • >TC006146 RA
      atgactaca cactctcac aaagtccgc cgcgtacaa 
      ggccacgaa tgcagcctt ccccaagaa gacgaactc 
      atcactctt tacataaac ggcggctta tttgtggac 
      tttcgccga aggttccgc cagtaccta atggtcagc 
      gctgcaaac cccgaaagc aaaaccgtc ctcaaatcc 
      accgcccaa attcgactc gaggaatcc agacacctc 
      cgacggtac taccacatg atccaccca ttcagcgaa 
      gccagaatg acatgggag atgataatg atcgttgtg 
      tacacaagt cttctcgtc ctcatgcct ctggaactg 
      ggaattcca atcacagag tcgttgatt ctctactcg 
      aagtttttc ctggacttg atttcgcta atggacatt 
      gcgttgttc ttctgtact ggatatttc gacgtcagg 
      aaacataag ataatattg aaacccacg ctggttttg 
      aaaaactac ctcttccct ttttttttc ttcgacttg 
      atctcgtct ttaccgtac aacatgtat atcgtccag 
      acgaagtcg tatgacttg cgctggttg aaatttttc 
      tacttggtt aaactgaca cgactacca actttgagc 
      aaatacatc aggaagttt tgcattcga atggaattg 
      tacagttac aaattgtca actgttaat tttgcgttg 
      tggtttttt acggccatt tggtgggcc acagcaatc 
      actttactc gtctattcc tcagtcgaa aattactct 
      ttacagtac tacatcgaa aatcccgac attttcaac 
      ccaactcta gaatcctgc taccaagtt gtgagagcg 
      tttatgttg gtggcaatg agcaaaata caaaccgat 
      agaccttta acaatgatt ttcaacata atttgtatc 
      ctaatcgga agcgttcta aacatggtc attttagcc 
      caagttatg caaatctat aagcggcac tcaagctcc 
      cgaaacaaa tacgacaat ttgctccaa gaaatcgca 
      gaatatatg aactacaag gaactgcca ccacctatc 
      aaaaacagg gtgttccgc tatgttgaa ttcaaattc 
      cagaaaaat atcttcaag gaagccgac atcctcaac 
      actttgtcc aaaatcctg aaacaggat attttgctg 
      cacaattgc aagaaaatg gtcgagaag gttgacttc 
      ttcaagggc ttgccaggt tctctaatt ttgcgtttg 
      gtgacgaag cttcgatcg gagatttat ttaccaaac 
      gacctcatt gtccaagct ggaattcca ggaaacgcc 
      atgtatttt atttatgtt ggatcggtc gccgtgtac 
      actatcaat gagcaagaa atttgtcat ttggaggat 
      ggaagttac ttcggcgaa atctcctta gttattaac 
      gaacctcga gtcgccagt gtcattgcg ataacaaac 
      tgtgaggtg ttcagactc agtcgtaag gattttatg 
      gaggcaatg gagccatat ccgaacttg actggaaaa 
      attcgcgaa aaagccttg agcagattg aagagaacc 
      aagcagatg actgacgat taccaccaa agaatcaaa 
      gctaaggtt tcagttgcg ttcgatgag ccaaccatt 
      ttggattaa
    mRNA sequence
    • >TC006146 RA
      tgtcaaatt ttcctcaca ttttgaaat gactacaca 
      ctctcacaa agtccgccg cgtacaagg ccacgaatg 
      cagccttcc ccaagaaga cgaactcat cactcttta 
      cataaacgg cggcttatt tgtggactt tcgccgaag 
      gttccgcca gtacctaat ggtcagcgc tgcaaaccc 
      cgaaagcaa aaccgtcct caaatccac cgcccaaat 
      tcgactcga ggaatccag acacctccg acggtacta 
      ccacatgat ccacccatt cagcgaagc cagaatgac 
      atgggagat gataatgat cgttgtgta cacaagtct 
      tctcgtcct catgcctct ggaactggg aattccaat 
      cacagagtc gttgattct ctactcgaa gtttttcct 
      ggacttgat ttcgctaat ggacattgc gttgttctt 
      ctgtactgg atatttcga cgtcaggaa acataagat 
      aatattgaa acccacgct ggttttgaa aaactacct 
      cttcccttt ttttttctt cgacttgat ctcgtcttt 
      accgtacaa catgtatat cgtccagac gaagtcgta 
      tgacttgcg ctggttgaa atttttcta cttggttaa 
      actgacacg actaccaac tttgagcaa atacatcag 
      gaagttttg cattcgaat ggaattgta cagttacaa 
      attgtcaac tgttaattt tgcgttgtg gttttttac 
      ggccatttg gtgggccac agcaatcac tttactcgt 
      ctattcctc agtcgaaaa ttactcttt acagtacta 
      catcgaaaa tcccgacat tttcaaccc aactctaga 
      atcctgcta ccaagttgt gagagcgtt tatgttggt 
      ggcaatgag caaaataca aaccgatag acctttaac 
      aatgatttt caacataat ttgtatcct aatcggaag 
      cgttctaaa catggtcat tttagccca agttatgca 
      aatctataa gcggcactc aagctcccg aaacaaata 
      cgacaattt gctccaaga aatcgcaga atatatgaa 
      ctacaagga actgccacc acctatcaa aaacagggt 
      gttccgcta tgttgaatt caaattcca gaaaaatat 
      cttcaagga agccgacat cctcaacac tttgtccaa 
      aatcctgaa acaggatat tttgctgca caattgcaa 
      gaaaatggt cgagaaggt tgacttctt caagggctt 
      gccaggttc tctaatttt gcgtttggt gacgaagct 
      tcgatcgga gatttattt accaaacga cctcattgt 
      ccaagctgg aattccagg aaacgccat gtattttat 
      ttatgttgg atcggtcgc cgtgtacac tatcaatga 
      gcaagaaat ttgtcattt ggaggatgg aagttactt 
      cggcgaaat ctccttagt tattaacga acctcgagt 
      cgccagtgt cattgcgat aacaaactg tgaggtgtt 
      cagactcag tcgtaagga ttttatgga ggcaatgga 
      gccatatcc gaacttgac tggaaaaat tcgcgaaaa 
      agccttgag cagattgaa gagaaccaa gcagatgac 
      tgacgatta ccaccaaag aatcaaagc taaggtttc 
      agttgcgtt cgatgagcc aaccatttt ggattaagc 
      tgcgtttta ttgtttaat caagtactt tcgttgacg 
      tgacttgtc caattccat tatttctta tcggcagag 
      gttgtaaca atagctttc gtttatcac ctccaaaca 
      atacaattt ctcctaatg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY