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Gene information
General information
TC006132 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2884315..2886039
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006132
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04073

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006132 RA
      MYSLYRAWL SLGPCPIPA LPPAPLNLT APAQGAPDV 
      ALQVGPQMT HFAAHRAVL ETHSGFFKA ALLNHADAA 
      PIAVPNVNV DEFSSLLTF MYTGYLDLN LSNIYNILL 
      ATHILHMPR ALEICRSFL LQSQPTLPR PAIIKPIPS 
      RKTAPPTQQ IHAPTYGHL EKSEATPFK TVVTQSRAS 
      TSKTEDAPT EKPKSEKPK KRRSETSAS GSEKVIIDI 
      ACCDGPVRF HRVINKNYG QQTQSENVY DQVDEVNRN 
      AAMNKAMTE NIRDRELSD ENQAGSEMF TCLYCNHTF 
      KSQYCYQKH ARRHLNPVS LDGKNVATV PKENKREVK 
      LLDMNVQYY PCKTCGSKF PSYYFVHKH RKMCHADEM 
      ADNNNKNNE MVPENSDSS SAIDVEAVN NDV
    CDS sequence
    • >TC006132 RA
      atgtactcg ctgtacagg gcctggctg tctttgggc 
      ccgtgcccc attccggcc ctgcctccg gcgcccctc 
      aacctcacg gcccccgca cagggcgcc cccgacgtc 
      gccctccaa gtcgggccc caaatgacg cactttgcc 
      gcgcacagg gccgtcctc gagacacac agcggattt 
      ttcaaagcg gctttactc aaccacgca gacgccgcc 
      cccattgcc gtccccaac gtcaacgtg gacgaattt 
      tcgtccctt ctcacgttc atgtacacc ggctacttg 
      gatctcaac ctcagcaat atatacaat atactactc 
      gccactcac atcctgcac atgccacgt gcccttgag 
      atttgtcgt tctttcctc ctccaaagc cagcccact 
      ttgccacgc cccgcaata attaaacca atcccgagt 
      cgtaaaacg gcacccccc acccaacaa atccacgcc 
      cccacctat ggtcacttg gagaaatct gaggctacg 
      ccttttaaa accgtcgtt acgcaaagt cgagcatcg 
      acgtcgaag accgaagac gcgccgacc gaaaaaccg 
      aaaagcgaa aaaccgaaa aaacgccgc agcgaaaca 
      agtgccagt ggaagcgag aaggttatt atcgacatc 
      gcttgctgc gatggacct gtgaggttt caccgtgtg 
      attaataaa aattacgga cagcaaacg caaagtgaa 
      aatgtttac gatcaagtg gacgaagtt aatcgaaat 
      gctgcaatg aataaggcg atgacggag aatatcaga 
      gatcgcgaa ttgtcggat gagaatcaa gcaggcagc 
      gagatgttc acttgtttg tactgtaac cacacgttc 
      aagtcgcag tattgttac cagaagcat gcccgaagg 
      cacttgaat cccgttagc cttgacggg aaaaatgtg 
      gcaacggtg cccaaagaa aacaagcga gaggtgaag 
      ttactagat atgaacgta cagtattat ccttgtaag 
      acgtgcggc tcgaaattc cctagttac tattttgtt 
      cacaagcac cgcaaaatg tgtcacgcc gacgaaatg 
      gccgataat aacaataaa aacaacgaa atggtgccc 
      gaaaacagt gacagcagc agcgccatc gacgtggaa 
      gctgtcaat aatgacgtt taa
    mRNA sequence
    • >TC006132 RA
      gggtagaaa gagcaaccc ccaccaagc caatataga 
      gagggtaat attttgaat tatggttat aaagcactg 
      acatttgat gaagatatc agtggtcgg tgacgagtg 
      agtgatatg tactcgctg tacagggcc tggctgtct 
      ttgggcccg tgccccatt ccggccctg cctccggcg 
      cccctcaac ctcacggcc cccgcacag ggcgccccc 
      gacgtcgcc ctccaagtc gggccccaa atgacgcac 
      tttgccgcg cacagggcc gtcctcgag acacacagc 
      ggatttttc aaagcggct ttactcaac cacgcagac 
      gccgccccc attgccgtc cccaacgtc aacgtggac 
      gaattttcg tcccttctc acgttcatg tacaccggc 
      tacttggat ctcaacctc agcaatata tacaatata 
      ctactcgcc actcacatc ctgcacatg ccacgtgcc 
      cttgagatt tgtcgttct ttcctcctc caaagccag 
      cccactttg ccacgcccc gcaataatt aaaccaatc 
      ccgagtcgt aaaacggca ccccccacc caacaaatc 
      cacgccccc acctatggt cacttggag aaatctgag 
      gctacgcct tttaaaacc gtcgttacg caaagtcga 
      gcatcgacg tcgaagacc gaagacgcg ccgaccgaa 
      aaaccgaaa agcgaaaaa ccgaaaaaa cgccgcagc 
      gaaacaagt gccagtgga agcgagaag gttattatc 
      gacatcgct tgctgcgat ggacctgtg aggtttcac 
      cgtgtgatt aataaaaat tacggacag caaacgcaa 
      agtgaaaat gtttacgat caagtggac gaagttaat 
      cgaaatgct gcaatgaat aaggcgatg acggagaat 
      atcagagat cgcgaattg tcggatgag aatcaagca 
      ggcagcgag atgttcact tgtttgtac tgtaaccac 
      acgttcaag tcgcagtat tgttaccag aagcatgcc 
      cgaaggcac ttgaatccc gttagcctt gacgggaaa 
      aatgtggca acggtgccc aaagaaaac aagcgagag 
      gtgaagtta ctagatatg aacgtacag tattatcct 
      tgtaagacg tgcggctcg aaattccct agttactat 
      tttgttcac aagcaccgc aaaatgtgt cacgccgac 
      gaaatggcc gataataac aataaaaac aacgaaatg 
      gtgcccgaa aacagtgac agcagcagc gccatcgac 
      gtggaagct gtcaataat gacgtttaa actcaataa 
      atgtaacaa actttggcg cgttttttt tttcaactc 
      acccggtat atcggcgtt ttcctcgtc actagtctc 
      cacattggc gttctcttt gtcttttct tctctcttt 
      cttcttttt ggtttcggc gatcgccgt tggagtcgc 
      agaggtcgt ttggtcgcc actgcaaat tcgaaaaaa 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY