| General information | |||
| TC006129 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 2828035..2832049 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC006129 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04072 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC006129 RA
MHRFSIVFL LFWLNLCQC KYLEGEIHT SDNWAFLAR FCFLSEDGQ FEYFLEFSD EQGNPNLLL YYDTDDQWP AVYKTNKNC YEKEAVLHV EQNQIINLT TTNRFYQEL SGCTSKPSP LPTPKPKPS TTLLPKKAT KSRKLRPTT TIKTTTIAL PTPRTTPTT TEDVTTKFV EDFNYTTLD LKSQWEHIE ITKAPIKKR AVRPPPTPQ IRGNTIMCH NARKFRSAR ERWWFIALS NCNGTRGIH VKYKILMTN GPPGDYWHE HFSADEFYI LPVLMAFSI AYSFLMLGI IVCSIELKS RQLLHTTYK IFVVSVIFQ LFGIILTSA AYLKYAVNG LGPSPVKRG GAIFMGVSE TLYMLLLLL LAKGFTITR GRLPLAASV KLTIFMCLY VVTYISIFI YEANVFDPG EVLYLYESP AGYGLIILR VLAWCMFVY STVFTLKHY PEKSNFYYP FNVCGTMWF IAGPAFILS ANSYIDKWI RESVVCAVL QFITFGGHL TFLILTMPS VANKNFPYH VRTTQIGIM ELTGTSGSS TIQQFGHHV YEPSGTVRE TVIVPLTRR TEEIFEGMY NQPVHNIRR ESLNEDLSN TRNLMMENV INWSLARNI AALEMPNFE RIERNSFVA EHSDNESVI TSRRSSQPN GHFDDYVRE VPIQLFTVS KMVTGNNDT PNGKTV
- >TC006129 RA
atgcaccgt ttttccatc gtttttctt ttattttgg ctgaatttg tgtcaatgc aaatatcta gaaggcgag atacacacc agtgacaat tgggctttt cttgcacgt ttttgcttc ttgtcggaa gacggccag tttgaatat tttctcgaa tttagtgac gaacaagga aatccaaat ttattgctt tattacgat acggacgac caatggccg gccgtatac aaaactaat aagaattgt tacgaaaaa gaagccgtg cttcacgtc gaacaaaac caaattatc aacctaaca acaactaac aggttttat caagaattg tcaggttgt acgagcaaa cctagccca cttccgaca cccaaaccc aaacctagc acaacctta ttaccgaaa aaagcaaca aaaagtcgc aaattacga cctacgact acgattaaa acaacaaca atcgcatta ccaacgcct agaacaaca cctactaca actgaagat gtgacaaca aaatttgtc gaagatttc aattacaca actttagat ttaaaaagc caatgggaa cacatcgaa atcactaaa gcaccaatc aagaaacga gccgtaagg ccaccaccc actccccaa atccgggga aataccatc atgtgtcac aacgcccgg aaattccgg agcgcgcgt gaaaggtgg tggttcata gctttgagt aattgtaac ggaacgaga ggaattcac gtcaagtat aagatttta atgacgaat ggaccaccg ggggattac tggcatgag catttctca gccgacgaa ttctatatt ctcccagtc ttgatggca ttctccatc gcctactcg ttcctaatg ttgggcata atcgtctgt tcaattgaa ctaaaatcg cgccaattg ttgcacaca acgtacaaa attttcgtc gtttcggtc attttccaa ctgtttgga attatttta acaagtgcc gcgtatttg aaatacgcc gttaatggg ctaggtcca agtcctgta aaacgaggc ggcgccatt ttcatgggc gtgtctgag acactatac atgcttctc ctgctctta ttggctaag ggctttacg ataacacgc ggccggctc ccattggct gccagtgtc aaacttacg attttcatg tgtttgtac gtggtgacg tacatttcg atttttatt tacgaggcc aatgttttc gatccgggc gaagtcctg tacttgtat gaatcaccg gctgggtat ggacttatc attttgagg gttttggcc tggtgtatg ttcgtctat tcgacggtt tttaccctc aaacattac ccggaaaag tcaaatttt tattatccg ttcaatgtg tgtggaacg atgtggttc atcgcaggg ccggctttt atactttcg gccaattcg tatattgac aagtggatt cgtgagtct gtcgtttgt gccgttttg cagtttatc accttcgga ggccacctc acgtttctg attttgacg atgccttca gtggccaat aaaaatttc ccgtaccac gtccggact actcagatc ggtataatg gaactaacc ggaacttcc ggcagtagt accatccaa cagttcggt catcacgtc tacgaaccg tcaggtaca gtccgtgaa acagtgatt gtacctttg acacgtcga actgaagaa atatttgaa ggaatgtac aatcaaccg gtgcacaat attcgtagg gagagttta aatgaagac ttgagtaac actagaaac ttaatgatg gaaaatgta attaattgg tcattggcg cgaaatatt gcagcttta gaaatgccc aatttcgag cgaattgaa cgcaacagt tttgttgca gaacattca gacaacgaa tcggttata accagtcga cgatcatcg caacccaat ggccacttt gacgattac gtccgagaa gtgccgatt caattgttt acggttagc aaaatggta accggcaat aatgacact cctaatgga aaaactgtt taa
- >TC006129 RA
tggctaaaa aataaattt cctccgacc ttcagctgt tttaaatgc gttgccttt ggcgtaacc atggctacc attgattcc gcacaaatg tacacaatc gaagatttt ccaaaatgc accgttttt ccatcgttt ttcttttat tttggctga atttgtgtc aatgcaaat atctagaag gcgagatac acaccagtg acaattggg cttttcttg cacgttttt gcttcttgt cggaagacg gccagtttg aatattttc tcgaattta gtgacgaac aaggaaatc caaatttat tgctttatt acgatacgg acgaccaat ggccggccg tatacaaaa ctaataaga attgttacg aaaaagaag ccgtgcttc acgtcgaac aaaaccaaa ttatcaacc taacaacaa ctaacaggt tttatcaag aattgtcag gttgtacga gcaaaccta gcccacttc cgacaccca aacccaaac ctagcacaa ccttattac cgaaaaaag caacaaaaa gtcgcaaat tacgaccta cgactacga ttaaaacaa caacaatcg cattaccaa cgcctagaa caacaccta ctacaactg aagatgtga caacaaaat ttgtcgaag atttcaatt acacaactt tagatttaa aaagccaat gggaacaca tcgaaatca ctaaagcac caatcaaga aacgagccg taaggccac cacccactc cccaaatcc ggggaaata ccatcatgt gtcacaacg cccggaaat tccggagcg cgcgtgaaa ggtggtggt tcatagctt tgagtaatt gtaacggaa cgagaggaa ttcacgtca agtataaga ttttaatga cgaatggac caccggggg attactggc atgagcatt tctcagccg acgaattct atattctcc cagtcttga tggcattct ccatcgcct actcgttcc taatgttgg gcataatcg tctgttcaa ttgaactaa aatcgcgcc aattgttgc acacaacgt acaaaattt tcgtcgttt cggtcattt tccaactgt ttggaatta ttttaacaa gtgccgcgt atttgaaat acgccgtta atgggctag gtccaagtc ctgtaaaac gaggcggcg ccattttca tgggcgtgt ctgagacac tatacatgc ttctcctgc tcttattgg ctaagggct ttacgataa cacgcggcc ggctcccat tggctgcca gtgtcaaac ttacgattt tcatgtgtt tgtacgtgg tgacgtaca tttcgattt ttatttacg aggccaatg ttttcgatc cgggcgaag tcctgtact tgtatgaat caccggctg ggtatggac ttatcattt tgagggttt tggcctggt gtatgttcg tctattcga cggttttta ccctcaaac attacccgg aaaagtcaa atttttatt atccgttca atgtgtgtg gaacgatgt ggttcatcg cagggccgg cttttatac tttcggcca attcgtata ttgacaagt ggattcgtg agtctgtcg tttgtgccg ttttgcagt ttatcacct tcggaggcc acctcacgt ttctgattt tgacgatgc cttcagtgg ccaataaaa atttcccgt accacgtcc ggactactc agatcggta taatggaac taaccggaa cttccggca gtagtacca tccaacagt tcggtcatc acgtctacg aaccgtcag gtacagtcc gtgaaacag tgattgtac ctttgacac gtcgaactg aagaaatat ttgaaggaa tgtacaatc aaccggtgc acaatattc gtagggaga gtttaaatg aagacttga gtaacacta gaaacttaa tgatggaaa atgtaatta attggtcat tggcgcgaa atattgcag ctttagaaa tgcccaatt tcgagcgaa ttgaacgca acagttttg ttgcagaac attcagaca acgaatcgg ttataacca gtcgacgat catcgcaac ccaatggcc actttgacg attacgtcc gagaagtgc cgattcaat tgtttacgg ttagcaaaa tggtaaccg gcaataatg acactccta atggaaaaa ctgtttaat tagcacaaa ttttgtaaa tttggaact ttttacaag tccagaatc gacgaacta aagcaatag gaatataca gttttgtgt aat
