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Gene information
General information
TC006129 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2828035..2832049
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006129
    iBeetle screen iB_04072

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006129 RA
      MHRFSIVFL LFWLNLCQC KYLEGEIHT SDNWAFLAR 
      FCFLSEDGQ FEYFLEFSD EQGNPNLLL YYDTDDQWP 
      AVYKTNKNC YEKEAVLHV EQNQIINLT TTNRFYQEL 
      SGCTSKPSP LPTPKPKPS TTLLPKKAT KSRKLRPTT 
      TIKTTTIAL PTPRTTPTT TEDVTTKFV EDFNYTTLD 
      LKSQWEHIE ITKAPIKKR AVRPPPTPQ IRGNTIMCH 
      NARKFRSAR ERWWFIALS NCNGTRGIH VKYKILMTN 
      GPPGDYWHE HFSADEFYI LPVLMAFSI AYSFLMLGI 
      IVCSIELKS RQLLHTTYK IFVVSVIFQ LFGIILTSA 
      AYLKYAVNG LGPSPVKRG GAIFMGVSE TLYMLLLLL 
      LAKGFTITR GRLPLAASV KLTIFMCLY VVTYISIFI 
      YEANVFDPG EVLYLYESP AGYGLIILR VLAWCMFVY 
      STVFTLKHY PEKSNFYYP FNVCGTMWF IAGPAFILS 
      ANSYIDKWI RESVVCAVL QFITFGGHL TFLILTMPS 
      VANKNFPYH VRTTQIGIM ELTGTSGSS TIQQFGHHV 
      YEPSGTVRE TVIVPLTRR TEEIFEGMY NQPVHNIRR 
      ESLNEDLSN TRNLMMENV INWSLARNI AALEMPNFE 
      RIERNSFVA EHSDNESVI TSRRSSQPN GHFDDYVRE 
      VPIQLFTVS KMVTGNNDT PNGKTV
    CDS sequence
    • >TC006129 RA
      atgcaccgt ttttccatc gtttttctt ttattttgg 
      ctgaatttg tgtcaatgc aaatatcta gaaggcgag 
      atacacacc agtgacaat tgggctttt cttgcacgt 
      ttttgcttc ttgtcggaa gacggccag tttgaatat 
      tttctcgaa tttagtgac gaacaagga aatccaaat 
      ttattgctt tattacgat acggacgac caatggccg 
      gccgtatac aaaactaat aagaattgt tacgaaaaa 
      gaagccgtg cttcacgtc gaacaaaac caaattatc 
      aacctaaca acaactaac aggttttat caagaattg 
      tcaggttgt acgagcaaa cctagccca cttccgaca 
      cccaaaccc aaacctagc acaacctta ttaccgaaa 
      aaagcaaca aaaagtcgc aaattacga cctacgact 
      acgattaaa acaacaaca atcgcatta ccaacgcct 
      agaacaaca cctactaca actgaagat gtgacaaca 
      aaatttgtc gaagatttc aattacaca actttagat 
      ttaaaaagc caatgggaa cacatcgaa atcactaaa 
      gcaccaatc aagaaacga gccgtaagg ccaccaccc 
      actccccaa atccgggga aataccatc atgtgtcac 
      aacgcccgg aaattccgg agcgcgcgt gaaaggtgg 
      tggttcata gctttgagt aattgtaac ggaacgaga 
      ggaattcac gtcaagtat aagatttta atgacgaat 
      ggaccaccg ggggattac tggcatgag catttctca 
      gccgacgaa ttctatatt ctcccagtc ttgatggca 
      ttctccatc gcctactcg ttcctaatg ttgggcata 
      atcgtctgt tcaattgaa ctaaaatcg cgccaattg 
      ttgcacaca acgtacaaa attttcgtc gtttcggtc 
      attttccaa ctgtttgga attatttta acaagtgcc 
      gcgtatttg aaatacgcc gttaatggg ctaggtcca 
      agtcctgta aaacgaggc ggcgccatt ttcatgggc 
      gtgtctgag acactatac atgcttctc ctgctctta 
      ttggctaag ggctttacg ataacacgc ggccggctc 
      ccattggct gccagtgtc aaacttacg attttcatg 
      tgtttgtac gtggtgacg tacatttcg atttttatt 
      tacgaggcc aatgttttc gatccgggc gaagtcctg 
      tacttgtat gaatcaccg gctgggtat ggacttatc 
      attttgagg gttttggcc tggtgtatg ttcgtctat 
      tcgacggtt tttaccctc aaacattac ccggaaaag 
      tcaaatttt tattatccg ttcaatgtg tgtggaacg 
      atgtggttc atcgcaggg ccggctttt atactttcg 
      gccaattcg tatattgac aagtggatt cgtgagtct 
      gtcgtttgt gccgttttg cagtttatc accttcgga 
      ggccacctc acgtttctg attttgacg atgccttca 
      gtggccaat aaaaatttc ccgtaccac gtccggact 
      actcagatc ggtataatg gaactaacc ggaacttcc 
      ggcagtagt accatccaa cagttcggt catcacgtc 
      tacgaaccg tcaggtaca gtccgtgaa acagtgatt 
      gtacctttg acacgtcga actgaagaa atatttgaa 
      ggaatgtac aatcaaccg gtgcacaat attcgtagg 
      gagagttta aatgaagac ttgagtaac actagaaac 
      ttaatgatg gaaaatgta attaattgg tcattggcg 
      cgaaatatt gcagcttta gaaatgccc aatttcgag 
      cgaattgaa cgcaacagt tttgttgca gaacattca 
      gacaacgaa tcggttata accagtcga cgatcatcg 
      caacccaat ggccacttt gacgattac gtccgagaa 
      gtgccgatt caattgttt acggttagc aaaatggta 
      accggcaat aatgacact cctaatgga aaaactgtt 
      taa
    mRNA sequence
    • >TC006129 RA
      tggctaaaa aataaattt cctccgacc ttcagctgt 
      tttaaatgc gttgccttt ggcgtaacc atggctacc 
      attgattcc gcacaaatg tacacaatc gaagatttt 
      ccaaaatgc accgttttt ccatcgttt ttcttttat 
      tttggctga atttgtgtc aatgcaaat atctagaag 
      gcgagatac acaccagtg acaattggg cttttcttg 
      cacgttttt gcttcttgt cggaagacg gccagtttg 
      aatattttc tcgaattta gtgacgaac aaggaaatc 
      caaatttat tgctttatt acgatacgg acgaccaat 
      ggccggccg tatacaaaa ctaataaga attgttacg 
      aaaaagaag ccgtgcttc acgtcgaac aaaaccaaa 
      ttatcaacc taacaacaa ctaacaggt tttatcaag 
      aattgtcag gttgtacga gcaaaccta gcccacttc 
      cgacaccca aacccaaac ctagcacaa ccttattac 
      cgaaaaaag caacaaaaa gtcgcaaat tacgaccta 
      cgactacga ttaaaacaa caacaatcg cattaccaa 
      cgcctagaa caacaccta ctacaactg aagatgtga 
      caacaaaat ttgtcgaag atttcaatt acacaactt 
      tagatttaa aaagccaat gggaacaca tcgaaatca 
      ctaaagcac caatcaaga aacgagccg taaggccac 
      cacccactc cccaaatcc ggggaaata ccatcatgt 
      gtcacaacg cccggaaat tccggagcg cgcgtgaaa 
      ggtggtggt tcatagctt tgagtaatt gtaacggaa 
      cgagaggaa ttcacgtca agtataaga ttttaatga 
      cgaatggac caccggggg attactggc atgagcatt 
      tctcagccg acgaattct atattctcc cagtcttga 
      tggcattct ccatcgcct actcgttcc taatgttgg 
      gcataatcg tctgttcaa ttgaactaa aatcgcgcc 
      aattgttgc acacaacgt acaaaattt tcgtcgttt 
      cggtcattt tccaactgt ttggaatta ttttaacaa 
      gtgccgcgt atttgaaat acgccgtta atgggctag 
      gtccaagtc ctgtaaaac gaggcggcg ccattttca 
      tgggcgtgt ctgagacac tatacatgc ttctcctgc 
      tcttattgg ctaagggct ttacgataa cacgcggcc 
      ggctcccat tggctgcca gtgtcaaac ttacgattt 
      tcatgtgtt tgtacgtgg tgacgtaca tttcgattt 
      ttatttacg aggccaatg ttttcgatc cgggcgaag 
      tcctgtact tgtatgaat caccggctg ggtatggac 
      ttatcattt tgagggttt tggcctggt gtatgttcg 
      tctattcga cggttttta ccctcaaac attacccgg 
      aaaagtcaa atttttatt atccgttca atgtgtgtg 
      gaacgatgt ggttcatcg cagggccgg cttttatac 
      tttcggcca attcgtata ttgacaagt ggattcgtg 
      agtctgtcg tttgtgccg ttttgcagt ttatcacct 
      tcggaggcc acctcacgt ttctgattt tgacgatgc 
      cttcagtgg ccaataaaa atttcccgt accacgtcc 
      ggactactc agatcggta taatggaac taaccggaa 
      cttccggca gtagtacca tccaacagt tcggtcatc 
      acgtctacg aaccgtcag gtacagtcc gtgaaacag 
      tgattgtac ctttgacac gtcgaactg aagaaatat 
      ttgaaggaa tgtacaatc aaccggtgc acaatattc 
      gtagggaga gtttaaatg aagacttga gtaacacta 
      gaaacttaa tgatggaaa atgtaatta attggtcat 
      tggcgcgaa atattgcag ctttagaaa tgcccaatt 
      tcgagcgaa ttgaacgca acagttttg ttgcagaac 
      attcagaca acgaatcgg ttataacca gtcgacgat 
      catcgcaac ccaatggcc actttgacg attacgtcc 
      gagaagtgc cgattcaat tgtttacgg ttagcaaaa 
      tggtaaccg gcaataatg acactccta atggaaaaa 
      ctgtttaat tagcacaaa ttttgtaaa tttggaact 
      ttttacaag tccagaatc gacgaacta aagcaatag 
      gaatataca gttttgtgt aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY