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Gene information
General information
TC006124 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2709545..2721174
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006124
    iBeetle screen iB_04071

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006124 RA
      MYETIEREG GDITAADMV LPEESSEIA RFYADSTVF 
      LTGATGFLG KMCLEKLLR DCYDVRKIY VMIRPKKGK 
      DIQTRFDEI FDGPNMEPL KRKNPNFGS KVVFINGDC 
      SLPDLGLND EDRAKLINE TNCIIHCAA TVRFDEKIR 
      TATHINVRA VIDLIQMAK QMKNLKAMI YVSTAFSNC 
      IRSEIREEF YPPPITGRK LLSLLDALD DDKLDKITP 
      IIMGDYPNT YVFTKAVAE DVIKTEGKT LPIAVFRPS 
      IVIASVKEP VAGWIDNLY GATGVLVGA ALGVLRSLH 
      GKIENGAEM VPADFVVNC AIASAWDIA SAKSINENR 
      EKNGKNQFE EEVPVYNFV SSPEAGITW DIYTKLAEK 
      HAKQVPSPL LVWHYFFAL RSSRIHHLI AVFFLHTIP 
      AYLVDFIAV CLGKKPMLV KGYQKINKF ADVISYFSS 
      REWKFTNAN VQSLWKKMG KRDREMFEF SMKNFNWDS 
      YFYTYVRGT RAYLLKDPL TTLPQGTVK YYKLMVLHY 
      VVMAILFFG FYKFLMFLL GLLF
    CDS sequence
    • >TC006124 RA
      atgtacgaa acaatcgaa cgcgagggc ggcgacatc 
      accgccgcc gacatggtc ctcccggaa gaatccagt 
      gaaattgcg cgattctac gccgattcg acggttttc 
      ctcaccgga gccaccgga tttttgggg aaaatgtgc 
      ctggaaaaa ctgctgagg gactgctac gacgtgcgg 
      aaaatctac gtcatgatc aggccgaaa aaagggaaa 
      gacatacag accagattc gacgagatt ttcgacgga 
      ccgaatatg gagccgctc aagcgcaaa aacccgaat 
      ttcgggtca aaggtcgtc ttcatcaac ggcgactgc 
      tcccttcct gacttgggt ttaaacgac gaagaccgc 
      gcaaaacta atcaacgaa acaaactgc ataatccac 
      tgtgctgcg acggtgcgt ttcgacgaa aaaatccgt 
      acggcaacg catatcaac gtccgtgca gttatagac 
      cttatccaa atggccaaa caaatgaag aatttaaag 
      gcgatgatt tacgtctcc accgccttc tcgaactgc 
      atccggagc gaaatccgg gaggaattc tacccgcct 
      ccaatcaca ggccggaaa ctcctctcc ctcttggac 
      gccctggac gacgacaag ctcgacaaa atcaccccg 
      ataatcatg ggtgattac cccaacact tacgtcttc 
      accaaagcc gtggccgaa gatgttata aaaacagaa 
      ggcaaaaca ctgccaatt gccgtcttc cggccctcg 
      attgttatt gcatcggtc aaggaaccg gtggctgga 
      tggattgat aatttgtac ggggccacg ggggtgctt 
      gtgggggct gccttgggg gttttgcgg agcctccac 
      gggaaaatc gaaaatggg gctgaaatg gtaccggcc 
      gattttgta gtcaactgt gccatagca tcggcctgg 
      gacatcgct tcggccaag tcaattaac gaaaatcgg 
      gagaaaaac gggaaaaat cagtttgag gaagaggtt 
      ccagtttat aattttgtc agttcgcct gaagccggg 
      attacgtgg gatatttac acgaaactg gccgagaaa 
      cacgcaaag caagtgcct agtccctta ctcgtctgg 
      cattacttt ttcgccctg cgatcgtcg cgaatccac 
      catttaatc gctgtgttc tttcttcac acaatcccg 
      gcgtatttg gtcgatttt attgccgtc tgtttgggc 
      aaaaaaccc atgctggtg aaaggttac cagaaaatc 
      aacaaattc gccgatgtt ataagttac ttcagttcg 
      cgcgaatgg aaattcaca aatgcgaac gtccaatcg 
      ctttggaag aaaatgggg aaacgtgat agggaaatg 
      tttgagttc agtatgaag aacttcaac tgggattcc 
      tatttttat acgtatgtt agaggaacg agggcctat 
      ctccttaaa gacccttta acgactttg cctcaaggg 
      actgtgaaa tattataag ttaatggtg ttgcattat 
      gtcgtaatg gcgattttg ttctttggt ttttataaa 
      tttttaatg ttcttgttg ggtttgttg ttttaa
    mRNA sequence
    • >TC006124 RA
      cacttctag taattttta taatagtag gaatggaaa 
      aagtgtcat ttttaagcc ttgtgggtt ttcccaaac 
      aaatgtcta aaaatcgca ccgagcgac cttctcttc 
      tctcttatt cgcggatca agggcgtgc tcgaagaga 
      tttcctgtt ttgaaaatt gttttgtga taggtatga 
      gtagacagc aacgtcgaa gtggggcaa aagcctacc 
      gtggagaac tcttgggcc aaacttgca atgtcagtt 
      ataaacttt acgtatacg catcgaaaa gtgaacgcg 
      cgtggtggg ttttgtgtg gttccgttc gaccacaat 
      cacaaacca tttgaaaga aaatgtacg aaacaatcg 
      aacgcgagg gcggcgaca tcaccgccg ccgacatgg 
      tcctcccgg aagaatcca gtgaaattg cgcgattct 
      acgccgatt cgacggttt tcctcaccg gagccaccg 
      gatttttgg ggaaaatgt gcctggaaa aactgctga 
      gggactgct acgacgtgc ggaaaatct acgtcatga 
      tcaggccga aaaaaggga aagacatac agaccagat 
      tcgacgaga ttttcgacg gaccgaata tggagccgc 
      tcaagcgca aaaacccga atttcgggt caaaggtcg 
      tcttcatca acggcgact gctcccttc ctgacttgg 
      gtttaaacg acgaagacc gcgcaaaac taatcaacg 
      aaacaaact gcataatcc actgtgctg cgacggtgc 
      gtttcgacg aaaaaatcc gtacggcaa cgcatatca 
      acgtccgtg cagttatag accttatcc aaatggcca 
      aacaaatga agaatttaa aggcgatga tttacgtct 
      ccaccgcct tctcgaact gcatccgga gcgaaatcc 
      gggaggaat tctacccgc ctccaatca caggccgga 
      aactcctct ccctcttgg acgccctgg acgacgaca 
      agctcgaca aaatcaccc cgataatca tgggtgatt 
      accccaaca cttacgtct tcaccaaag ccgtggccg 
      aagatgtta taaaaacag aaggcaaaa cactgccaa 
      ttgccgtct tccggccct cgattgtta ttgcatcgg 
      tcaaggaac cggtggctg gatggattg ataatttgt 
      acggggcca cgggggtgc ttgtggggg ctgccttgg 
      gggttttgc ggagcctcc acgggaaaa tcgaaaatg 
      gggctgaaa tggtaccgg ccgattttg tagtcaact 
      gtgccatag catcggcct gggacatcg cttcggcca 
      agtcaatta acgaaaatc gggagaaaa acgggaaaa 
      atcagtttg aggaagagg ttccagttt ataattttg 
      tcagttcgc ctgaagccg ggattacgt gggatattt 
      acacgaaac tggccgaga aacacgcaa agcaagtgc 
      ctagtccct tactcgtct ggcattact ttttcgccc 
      tgcgatcgt cgcgaatcc accatttaa tcgctgtgt 
      tctttcttc acacaatcc cggcgtatt tggtcgatt 
      ttattgccg tctgtttgg gcaaaaaac ccatgctgg 
      tgaaaggtt accagaaaa tcaacaaat tcgccgatg 
      ttataagtt acttcagtt cgcgcgaat ggaaattca 
      caaatgcga acgtccaat cgctttgga agaaaatgg 
      ggaaacgtg atagggaaa tgtttgagt tcagtatga 
      agaacttca actgggatt cctattttt atacgtatg 
      ttagaggaa cgagggcct atctcctta aagaccctt 
      taacgactt tgcctcaag ggactgtga aatattata 
      agttaatgg tgttgcatt atgtcgtaa tggcgattt 
      tgttctttg gtttttata aatttttaa tgttcttgt 
      tgggtttgt tgttttaag aaggtgtat gtgattaaa 
      taaatttat ttgtctata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY