Status
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Gene information
General information
TC033959
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006122,TC006121
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2698045..2706605
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033959
    iBeetle screen iB_04070

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033959 RA
      METSQIANF FKNQTLFLT GGTGFVGKL ILEKLLREC 
      PEIKKIILI LRPKKGKTS QERFDQLFD LPCFELLKS 
      TNINFSEKV FLVDGDCQE PFLGLSAQN LDLLREEVT 
      CVIHAAANV KFDQSLKEA AFNVRATRD LLELAKQMP 
      NLKSFVYVS TAYSNCLNA HIKEDFYKP PLKHDNLLW 
      LVNFLNDDV LTKITPSLL GPWPNTYVY TKCISEDLV 
      KSASTSLPI AIVRPAIII SAFKEPIPG WIDNFYGVV 
      GLVLGVGLG VIHSLHLKL DAKALLVPV DYVANLILA 
      VTCKTGRDK SKKTRIYNY VKMRRNDLT WGEFLKIIE 
      GYYWNYASV KCIWYYSCQ YVESKRWHD IRIFFLHTV 
      LGYVVDFVL FCLGRPTLA VKNYERLNK LLECTSFFA 
      IRNWSFDDS NVRRLWNEL SDDDKKTFE FYCDQMDLN 
      SYAFDSIFG GRQFLLKDS QDTIPRARK KVKILFVAH 
      YTLLAIFCA QNLDLLREE VTCVIHAAA NVKFDQSLK 
      EAAFNVRAT RDLLELAKQ ILNLKSFVY VSTAYSNCL 
      NPHIREDFY NQPLKPENL LSVVNSLDN DVLTKITPS 
      LLGPWPNTY IYTKSITED LVKSASTSL PIAIVRPAI 
      SSIKEPVPG WIDNFYGVV GIVLAASLG VLRSLNAKL 
      DAIAPIVPV DYVANVIIA ATYQFMKKV EPECWNAGY 
      DKVVWYFGF KLRENKTWH NIYIFFTHN VVSHIVDFV 
      LLCVGRPTL AVKNYERLN KLLDLISYF STRSWTFDT 
      DNVTKLWHE MSDDDKNRF EFHLDSADW NLLAHGSVF 
      GEKKFLLRE SINIFSEPL LVLTMEPNQ IVNFFKNQT 
      LFLTGGTGF VGKLILEKL LRECPEIKK IVLILRPKK 
      GKTSQERFD QLFDLPCFE LLKSMKINI SEKVFLVDG 
      DCQEPFLGL SAQNLDLLR EEVTCVIHA AANVKFDQS 
      LKEAAFNVR GTRDLLELA KQMPNLKSF VYVSTAYSN 
      CLNPHIRED FYEPPLKPE NLLSVVNIL DDDVLTEIT 
      PFVLGPWPN TYIYTKSIT EDLVKSAGT SLPIAIVRP 
      AISSIKEPI PGWIDNFYG VVGIVLAAS LGVLRSLNA 
      KLDAIAPLV PVDYVANVI IAATYKTAR DQPKFPVIY 
      NYVHFGKKS ELTWDEFMK KVEPECWNA ASDKVIWYP 
      VFKLQENKT WHNIYIFFT HTVVAYIVD FILFCIGRP 
      TLAVKNYAR LNKLLALIS YFSTRSWTF DTDNVTKLW 
      HEMSDDDKN KFEFLLDTI DWNSFVHDC VVGGRKFLL 
      KETPDTIPR GKRKLRILF FAYYTLMAV FWFLMYKLQ 
      SNTMSSSEI NTFFKNQTV FLLGGTGFL GKTLIEKLL 
      RFDQIAKIF LLVRPKKDR SLDERFDDL FNFPCFERL 
      KKENPEFRK KIVFVSGDC ERPNLGISP ETEDVLIAE 
      TSIVIHAAA NVKFDQPLR TAAYINVRS TWDCLKLAK 
      KMPKLKAFI YVSTAYSNC PYIDIEEKF YTPLVTPRT 
      LLQIVDSLD DEILDNITP GLLGKWPNA YVFTKTIAE 
      DLIKSEGKN LPIGIVRPA IVVTCAEEP VPGWIDNFY 
      GVIGMTVAI ALGIVRSVH AIKKAPCHL VPCDYVCNF 
      ILASTWNVA QETTDQITI FNYSGSNKN LISWQKYAQ 
      LIETCAWDY PSTKILWYY GFSFTDSKL YHDVQTFLF 
      HTVVFSLVD LVLFCVGKK PMAVKNVKR LNYTLSKVS 
      YFTLNKFSF KHENVDKLW AKMKSDDKN LFRFDMSDF 
      HWGNYLRKT VEGGRTYLL KESQETIPL GHKKVRILR 
      VAYYSSLVL LFYCLFLFV KLILHFVM
    CDS sequence
    • >TC033959 RA
      atggaaacg agtcaaatc gccaacttc ttcaaaaac 
      caaacactt ttcctaacc ggaggaacc ggttttgtt 
      ggcaaacta atcctcgaa aaactcctt cgggaatgt 
      cccgaaatc aaaaaaatc atcctaatt ttgcgcccc 
      aaaaaaggc aaaacgtcc caagaacga ttcgaccaa 
      ctctttgac ttgccttgt ttcgaattg ctcaaatcc 
      acgaatatc aacttttcg gagaaagtc tttttggtc 
      gatggtgac tgtcaggag ccttttttg ggcctcagt 
      gcccaaaat ttggacttg ttgcgtgag gaagtgacg 
      tgtgttatc cacgctgct gccaacgtt aaattcgac 
      caaagtctc aaagaggca gctttcaat gttcgagcg 
      actcgagat ttgctcgag ttggccaaa cagatgcca 
      aacttgaag agcttcgtt tacgtctcg actgcttat 
      tccaactgt ttgaacgct cacattaag gaagatttc 
      tacaaacca cccttgaaa cacgataat ttactatgg 
      ttggtcaat tttctcaac gatgatgtt ttgacaaaa 
      ataacaccc tctttgttg ggcccttgg cccaacact 
      tacgtctac accaaatgc atatctgag gatttagtc 
      aaatcagct agtactagc ctcccgatt gccatagta 
      cgcccagct atcattata agtgcgttc aaagaacca 
      atccctggc tggattgat aacttctat ggagttgtt 
      ggtctagtt ttaggtgtc ggtttgggc gtgatacac 
      agcttgcat ctaaaatta gatgcaaaa gcgttatta 
      gtacctgtc gattatgtt gcaaattta attctcgca 
      gtcacttgt aaaacagga cgtgacaaa tcgaaaaaa 
      acacgcatt tataattac gtaaaaatg aggaggaat 
      gacttgact tggggagaa tttttgaaa ataatcgaa 
      gggtactat tggaattat gcgtccgtt aaatgtatc 
      tggtactac agttgtcaa tacgtcgag agcaaacgc 
      tggcatgac atacgtatt tttttcttg cacacggtt 
      ttgggatac gtcgtcgat tttgtgttg ttttgtctc 
      ggaagacct acactcgcc gtcaaaaat tacgaaaga 
      ttgaacaaa cttttggaa tgcacttcg ttttttgcg 
      atccggaat tggagtttt gacgattcc aacgtacga 
      cgattatgg aatgaattg agtgatgac gataagaaa 
      acttttgaa ttttattgc gaccaaatg gatttgaat 
      agttatgcc ttcgatagt atttttgga ggaaggcaa 
      tttttgttg aaggactca caagacacg attcctcgt 
      gcgaggaaa aaagtgaag attttattt gtggcacat 
      tatactctc cttgcaatc ttttgtgcc caaaatttg 
      gacttgttg cgcgaggaa gtgacgtgt gttatccac 
      gctgctgcc aatgttaaa ttcgaccaa agtctcaaa 
      gaggcagct ttcaatgtt cgagcgact cgagatttg 
      ctcgaattg gccaaacag atactaaac ttgaagagt 
      ttcgtctac gtctcgacg gcttattcc aactgtctt 
      aaccctcac attagggaa gatttttac aaccaaccc 
      ctaaaacct gaaaactta ctctcggtt gtgaattcc 
      ctggacaat gatgttttg acgaaaata acaccatct 
      ttgttgggc ccttggccc aacacttac atctacacc 
      aaatccata actgaggat ttagtcaaa tcagctagt 
      actagcctc ccgattgcc atagtacgc ccagctatc 
      agttcgatc aaagaacca gtccctggc tggattgac 
      aacttttac ggagtcgtt ggtatagtt ctagctgca 
      tctttgggc gttttgagg agtctgaat gcaaaacta 
      gacgccata gcacccata gtaccggtc gattatgtc 
      gccaatgta atcatagca gcgacgtac caatttatg 
      aaaaaagtg gagcccgaa tgttggaac gcagggtat 
      gataaagtc gtttggtac ttcggcttc aaactacgc 
      gaaaataag acttggcac aacatttac atatttttc 
      acgcacaat gttgtatct cacatcgtt gatttcgtc 
      ctgctctgt gtcggaaga ccgacttta gctgtgaaa 
      aattacgaa cgtctcaat aaactactc gacttgatt 
      tcgtatttc tcgacccga agctggact tttgacact 
      gataatgtt acaaaattg tggcacgaa atgagcgat 
      gatgataag aacagattt gagtttcat ttggattcg 
      gccgactgg aatttgctt gcccacggc agtgttttc 
      ggagagaaa aaattcctt ctaagggaa agcatcaat 
      atattttcc gaacccttg ttagttctc actatggaa 
      ccgaaccaa atagtcaat ttcttcaaa aaccaaaca 
      cttttccta accggagga accggcttt gttggcaaa 
      ctgatcctc gaaaaactc cttcgagaa tgtcccgaa 
      ataaaaaaa atcgtccta attttgcgt ccaaaaaaa 
      gggaaaacg tcccaagaa cgattcgac caactcttc 
      gacttgcct tgtttcgaa ttactcaaa tcgatgaaa 
      atcaacatt tcggagaaa gtttttttg gtggacggt 
      gattgtcaa gagcctttc ttgggcctc agtgcccaa 
      aatttggac ttgttgcgc gaggaagtg acgtgtgtt 
      atccacgct gctgccaat gttaaattc gaccaaagt 
      ctcaaagag gcagctttc aatgttcga gggactcga 
      gatttgctc gagttggcc aaacagatg ccaaacttg 
      aagagtttt gtctacgtc tcgacggct tattccaac 
      tgtcttaac cctcacatt agggaagat ttttacgaa 
      ccaccccta aaacctgaa aacttactc tcggtggtg 
      aatatcctc gacgacgat gttttgacg gaaataaca 
      ccttttgtg ttgggccct tggcccaac acttacatc 
      tacaccaaa tccataact gaggattta gtcaaatca 
      gctggtact agcctcccg attgccata gtacgccca 
      gctatcagt tcgatcaaa gaaccaatt cccggctgg 
      attgacaac ttttacgga gttgttggt atagttcta 
      gctgcatct ttgggcgtt ttgaggagt ctgaatgca 
      aaactagac gccatagca cccctagta cccgtcgat 
      tatgtcgcc aatgtaatc atagcagcg acttataaa 
      accgcaaga gatcaaccg aaattcccg gtcatttac 
      aactacgtc cactttggc aaaaaaagt gaactcact 
      tgggatgaa tttatgaaa aaagttgag cccgaatgt 
      tggaacgca gcgtctgat aaagtcatt tggtacccc 
      gtcttcaaa ctacaagaa aataagact tggcacaac 
      atttatata tttttcacg cacactgtt gtggcttac 
      attgtagat ttcatcctg ttttgtatc ggaagaccg 
      actttagct gtgaaaaat tacgcacgt ctcaataaa 
      ctgctcgcc ttgatttcg tatttctcg acccgaagc 
      tggactttt gacactgat aatgttaca aaattgtgg 
      cacgaaatg agcgatgat gataagaac aaatttgag 
      tttcttttg gatacgatc gactggaat tcgtttgtc 
      cacgactgt gttgtcgga ggtagaaaa ttccttctg 
      aaggaaact cccgatact attccgaga gggaaacgg 
      aaacttaga attttgttt tttgcttat tatactcta 
      atggccgtg ttttggttt ctcatgtat aaactacag 
      tccaataca atgtcgtcg agcgaaatt aatactttc 
      ttcaaaaac caaaccgtc ttccttctc ggtggtacg 
      ggttttttg ggcaaaacc ctaatcgag aaactcctg 
      cgattcgac caaatcgca aaaattttt cttttagtg 
      cgccccaaa aaagacaga agcctcgac gaacgcttc 
      gacgactta ttcaatttt ccgtgtttc gagcgtttg 
      aaaaaagaa aatccggaa tttcgaaaa aaaattgtc 
      ttcgtcagt ggtgattgt gagcgcccc aatttaggg 
      atttccccc gagactgaa gatgtgcta attgccgaa 
      acaagtatc gtgatacat gcagccgct aatgtcaag 
      tttgaccaa cctttacga acagccgcc tacatcaat 
      gtacgaagt acttgggat tgcctaaag ttggccaaa 
      aaaatgcca aaattaaaa gcctttatt tacgtgtcc 
      accgcctat tccaactgt ccttacatt gatattgaa 
      gaaaagttt tacacacca ttggtgact ccccggact 
      ttacttcaa attgtcgac tcgttggac gacgaaatt 
      ttggataac ataactcca ggattattg ggcaaatgg 
      cccaatgct tatgttttc accaaaaca atagctgag 
      gatttgatc aaatctgag ggaaaaaac ctcccaata 
      ggaatagta cgaccagcg atagtggtg acatgtgcc 
      gaagaacca gtgcctgga tggattgac aatttctac 
      ggtgttata ggaatgaca gtagctata gctctaggt 
      attgtcagg agtgtccac gctataaaa aaggcccct 
      tgtcatttg gtaccttgt gattatgtt tgtaatttt 
      attctagct tctacttgg aatgtggcc caagaaaca 
      acggatcaa attacaatt tttaattat tccgggtcg 
      aacaaaaac ctcatatct tggcaaaaa tatgcccaa 
      ttgatcgaa acctgtgct tgggattac ccctccact 
      aaaatttta tggtactat gggttcagt ttcactgat 
      agtaaattg taccacgac gtccaaacg tttcttttt 
      cacaccgtt gtattctcg ttggtcgac ttggtccta 
      ttttgtgtt ggcaaaaaa ccaatggct gtgaaaaac 
      gtcaaaaga ctgaactac accctaagc aaagtgtcc 
      tatttcacg ttgaacaag ttcagtttc aagcatgag 
      aatgtcgac aaactttgg gcaaaaatg aaaagtgat 
      gataaaaat ttgtttcgg tttgatatg agcgacttt 
      cattggggg aattatctc aggaagaca gttgaaggg 
      gggcgaact tacttactg aaggagtcg caagaaacc 
      attcctttg gggcacaaa aaagtcagg attttgaga 
      gtcgcttat tactcgtcg cttgttttg ttgttttac 
      tgtcttttc ctttttgtt aaactcatt ttacacttt 
      gttatgtaa
    mRNA sequence
    • >TC033959 RA
      acactcagc aatggaaac gagtcaaat cgccaactt 
      cttcaaaaa ccaaacact tttcctaac cggaggaac 
      cggttttgt tggcaaact aatcctcga aaaactcct 
      tcgggaatg tcccgaaat caaaaaaat catcctaat 
      tttgcgccc caaaaaagg caaaacgtc ccaagaacg 
      attcgacca actctttga cttgccttg tttcgaatt 
      gctcaaatc cacgaatat caacttttc ggagaaagt 
      ctttttggt cgatggtga ctgtcagga gcctttttt 
      gggcctcag tgcccaaaa tttggactt gttgcgtga 
      ggaagtgac gtgtgttat ccacgctgc tgccaacgt 
      taaattcga ccaaagtct caaagaggc agctttcaa 
      tgttcgagc gactcgaga tttgctcga gttggccaa 
      acagatgcc aaacttgaa gagcttcgt ttacgtctc 
      gactgctta ttccaactg tttgaacgc tcacattaa 
      ggaagattt ctacaaacc acccttgaa acacgataa 
      tttactatg gttggtcaa ttttctcaa cgatgatgt 
      tttgacaaa aataacacc ctctttgtt gggcccttg 
      gcccaacac ttacgtcta caccaaatg catatctga 
      ggatttagt caaatcagc tagtactag cctcccgat 
      tgccatagt acgcccagc tatcattat aagtgcgtt 
      caaagaacc aatccctgg ctggattga taacttcta 
      tggagttgt tggtctagt tttaggtgt cggtttggg 
      cgtgataca cagcttgca tctaaaatt agatgcaaa 
      agcgttatt agtacctgt cgattatgt tgcaaattt 
      aattctcgc agtcacttg taaaacagg acgtgacaa 
      atcgaaaaa aacacgcat ttataatta cgtaaaaat 
      gaggaggaa tgacttgac ttggggaga atttttgaa 
      aataatcga agggtacta ttggaatta tgcgtccgt 
      taaatgtat ctggtacta cagttgtca atacgtcga 
      gagcaaacg ctggcatga catacgtat ttttttctt 
      gcacacggt tttgggata cgtcgtcga ttttgtgtt 
      gttttgtct cggaagacc tacactcgc cgtcaaaaa 
      ttacgaaag attgaacaa acttttgga atgcacttc 
      gttttttgc gatccggaa ttggagttt tgacgattc 
      caacgtacg acgattatg gaatgaatt gagtgatga 
      cgataagaa aacttttga attttattg cgaccaaat 
      ggatttgaa tagttatgc cttcgatag tatttttgg 
      aggaaggca atttttgtt gaaggactc acaagacac 
      gattcctcg tgcgaggaa aaaagtgaa gattttatt 
      tgtggcaca ttatactct ccttgcaat cttttgtgc 
      ccaaaattt ggacttgtt gcgcgagga agtgacgtg 
      tgttatcca cgctgctgc caatgttaa attcgacca 
      aagtctcaa agaggcagc tttcaatgt tcgagcgac 
      tcgagattt gctcgaatt ggccaaaca gatactaaa 
      cttgaagag tttcgtcta cgtctcgac ggcttattc 
      caactgtct taaccctca cattaggga agattttta 
      caaccaacc cctaaaacc tgaaaactt actctcggt 
      tgtgaattc cctggacaa tgatgtttt gacgaaaat 
      aacaccatc tttgttggg cccttggcc caacactta 
      catctacac caaatccat aactgagga tttagtcaa 
      atcagctag tactagcct cccgattgc catagtacg 
      cccagctat cagttcgat caaagaacc agtccctgg 
      ctggattga caactttta cggagtcgt tggtatagt 
      tctagctgc atctttggg cgttttgag gagtctgaa 
      tgcaaaact agacgccat agcacccat agtaccggt 
      cgattatgt cgccaatgt aatcatagc agcgacgta 
      ccaatttat gaaaaaagt ggagcccga atgttggaa 
      cgcagggta tgataaagt cgtttggta cttcggctt 
      caaactacg cgaaaataa gacttggca caacattta 
      catattttt cacgcacaa tgttgtatc tcacatcgt 
      tgatttcgt cctgctctg tgtcggaag accgacttt 
      agctgtgaa aaattacga acgtctcaa taaactact 
      cgacttgat ttcgtattt ctcgacccg aagctggac 
      ttttgacac tgataatgt tacaaaatt gtggcacga 
      aatgagcga tgatgataa gaacagatt tgagtttca 
      tttggattc ggccgactg gaatttgct tgcccacgg 
      cagtgtttt cggagagaa aaaattcct tctaaggga 
      aagcatcaa tatattttc cgaaccctt gttagttct 
      cactatgga accgaacca aatagtcaa tttcttcaa 
      aaaccaaac acttttcct aaccggagg aaccggctt 
      tgttggcaa actgatcct cgaaaaact ccttcgaga 
      atgtcccga aataaaaaa aatcgtcct aattttgcg 
      tccaaaaaa agggaaaac gtcccaaga acgattcga 
      ccaactctt cgacttgcc ttgtttcga attactcaa 
      atcgatgaa aatcaacat ttcggagaa agttttttt 
      ggtggacgg tgattgtca agagccttt cttgggcct 
      cagtgccca aaatttgga cttgttgcg cgaggaagt 
      gacgtgtgt tatccacgc tgctgccaa tgttaaatt 
      cgaccaaag tctcaaaga ggcagcttt caatgttcg 
      agggactcg agatttgct cgagttggc caaacagat 
      gccaaactt gaagagttt tgtctacgt ctcgacggc 
      ttattccaa ctgtcttaa ccctcacat tagggaaga 
      tttttacga accacccct aaaacctga aaacttact 
      ctcggtggt gaatatcct cgacgacga tgttttgac 
      ggaaataac accttttgt gttgggccc ttggcccaa 
      cacttacat ctacaccaa atccataac tgaggattt 
      agtcaaatc agctggtac tagcctccc gattgccat 
      agtacgccc agctatcag ttcgatcaa agaaccaat 
      tcccggctg gattgacaa cttttacgg agttgttgg 
      tatagttct agctgcatc tttgggcgt tttgaggag 
      tctgaatgc aaaactaga cgccatagc acccctagt 
      acccgtcga ttatgtcgc caatgtaat catagcagc 
      gacttataa aaccgcaag agatcaacc gaaattccc 
      ggtcattta caactacgt ccactttgg caaaaaaag 
      tgaactcac ttgggatga atttatgaa aaaagttga 
      gcccgaatg ttggaacgc agcgtctga taaagtcat 
      ttggtaccc cgtcttcaa actacaaga aaataagac 
      ttggcacaa catttatat atttttcac gcacactgt 
      tgtggctta cattgtaga tttcatcct gttttgtat 
      cggaagacc gactttagc tgtgaaaaa ttacgcacg 
      tctcaataa actgctcgc cttgatttc gtatttctc 
      gacccgaag ctggacttt tgacactga taatgttac 
      aaaattgtg gcacgaaat gagcgatga tgataagaa 
      caaatttga gtttctttt ggatacgat cgactggaa 
      ttcgtttgt ccacgactg tgttgtcgg aggtagaaa 
      attccttct gaaggaaac tcccgatac tattccgag 
      agggaaacg gaaacttag aattttgtt ttttgctta 
      ttatactct aatggccgt gttttggtt tctcatgta 
      taaactaca gtccaatac aatgtcgtc gagcgaaat 
      taatacttt cttcaaaaa ccaaaccgt cttccttct 
      cggtggtac gggtttttt gggcaaaac cctaatcga 
      gaaactcct gcgattcga ccaaatcgc aaaaatttt 
      tcttttagt gcgccccaa aaaagacag aagcctcga 
      cgaacgctt cgacgactt attcaattt tccgtgttt 
      cgagcgttt gaaaaaaga aaatccgga atttcgaaa 
      aaaaattgt cttcgtcag tggtgattg tgagcgccc 
      caatttagg gatttcccc cgagactga agatgtgct 
      aattgccga aacaagtat cgtgataca tgcagccgc 
      taatgtcaa gtttgacca acctttacg aacagccgc 
      ctacatcaa tgtacgaag tacttggga ttgcctaaa 
      gttggccaa aaaaatgcc aaaattaaa agcctttat 
      ttacgtgtc caccgccta ttccaactg tccttacat 
      tgatattga agaaaagtt ttacacacc attggtgac 
      tccccggac tttacttca aattgtcga ctcgttgga 
      cgacgaaat tttggataa cataactcc aggattatt 
      gggcaaatg gcccaatgc ttatgtttt caccaaaac 
      aatagctga ggatttgat caaatctga gggaaaaaa 
      cctcccaat aggaatagt acgaccagc gatagtggt 
      gacatgtgc cgaagaacc agtgcctgg atggattga 
      caatttcta cggtgttat aggaatgac agtagctat 
      agctctagg tattgtcag gagtgtcca cgctataaa 
      aaaggcccc ttgtcattt ggtaccttg tgattatgt 
      ttgtaattt tattctagc ttctacttg gaatgtggc 
      ccaagaaac aacggatca aattacaat ttttaatta 
      ttccgggtc gaacaaaaa cctcatatc ttggcaaaa 
      atatgccca attgatcga aacctgtgc ttgggatta 
      cccctccac taaaatttt atggtacta tgggttcag 
      tttcactga tagtaaatt gtaccacga cgtccaaac 
      gtttctttt tcacaccgt tgtattctc gttggtcga 
      cttggtcct attttgtgt tggcaaaaa accaatggc 
      tgtgaaaaa cgtcaaaag actgaacta caccctaag 
      caaagtgtc ctatttcac gttgaacaa gttcagttt 
      caagcatga gaatgtcga caaactttg ggcaaaaat 
      gaaaagtga tgataaaaa tttgtttcg gtttgatat 
      gagcgactt tcattgggg gaattatct caggaagac 
      agttgaagg ggggcgaac ttacttact gaaggagtc 
      gcaagaaac cattccttt ggggcacaa aaaagtcag 
      gattttgag agtcgctta ttactcgtc gcttgtttt 
      gttgtttta ctgtctttt cctttttgt taaactcat 
      tttacactt tgttatgta attttctaa attttttct 
      gtagtttta tttcatttc cttaaacta aaatcccta 
      ccaccaa
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