Status
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Gene information
General information
TC006112 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2576095..2587206
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006112
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04067

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006112 RA
      MAFLDHFRK GDDVKRLMI RYLRSPKMA ETVKKSRIV 
      SDNDDDLEE ALMEEFNQR LKKCMSQDD LQQTAEEEE 
      QENINEKFI QAEKQNGYP KDSIYENVG HQTNKTELE 
      KSDQYIDMS GNPEVKDEE VAVYEDIDD DLPIKEKPN 
      LILTDLCDV KPNDSSETK SVKDRMLLS TDVAPCLLF 
      TQTVTSPML TPSEENIDF LKGFRREST DETKDENIY 
      ENTEFLKNQ TNEAIYENL KENIYENVK ELNGKQEAG 
      EEIIYQNVE ELRDNNEDE IEEEKSEVM DEPVDDDVT 
      NQIDFESEE STQVTESYV ETTTFESNS SEKGIKKKK 
      SKKSRKEEK ENISVNGTN TVCEDSFYN VNVKILCRS 
      FGDLTKISD ESQKRTRTK SLSDASNKI ENVFFGVSV 
      RALFFACVV VLTQREKFY CNSGDCRAK SADGKSSFS 
      KFDALQKKN VLHIRSSDS SKAQEKFNG AQIDPSNCR 
      SCGKAVFQM EQIKAEKAV WHKNCFRCT ECNKQLNVD 
      TYQSNEGSL YCKPHFKAL FAPKAVEDD ASPKPRKPE 
      LIIRESQPI ELPPDVVRA SDKPDLGLE ELQSLNVKE 
      RFQVFEHHQ SETSEERIP SHVNVKRSP SILSKLAKF 
      QAKGMDIGV ADDSLNGIP IEESSSEEE EEEDDGIEG 
      EDADLIRAK RVQKEKPFH FTGMSDVKS RWEQGEQTT 
      SRDERREER KQEIQSIRN RLFMGKQGK MKEAYQQAV 
      MESESCTNL KKETPIEVC DTKSLKEKF EKGEVVQER 
      AREKDAEEN EVYESEISK KSRSLFLEL DANASKPPQ 
      ITPITTPKI DVKKARDNY LSRSASEDV VKSSVNVVD 
      DVQIRTSDI QQRFKFFET YKEPEKEKK SFRITPPRE 
      GQVKAPTPD REVYRDPEI VRSEEIIED SVIAKETHT 
      ATKMLNKFR QMEENLAKE PQPIGPKPL KRFTPPPEP 
      TRPESNSEA ESDSGEETE EEDEIDRGK IADEDLIEA 
      QKAARAKQL RAKFERWEA NEIKKEQNN SSVNIIEEL 
      GEEQSQIES TKTLRARFE SMRESSTET RQPRVKVNR 
      FVELPNVTE RCESCNSRV YPLEKISVH NHIYHKSCF 
      KCMECNCVL RMDSYSYNQ GLLYCMPHF KRLFISKGN 
      YDTGFGLGQ HKEKWNATI A
    CDS sequence
    • >TC006112 RA
      atggcattt ttggaccat tttaggaaa ggcgacgac 
      gtgaagcgt ttaatgatt agatatttg cgttcaccg 
      aaaatggcc gaaactgta aaaaagtcc cgaattgta 
      agtgacaac gacgacgat ctcgaggaa gctttgatg 
      gaagaattt aaccagagg ctaaaaaaa tgcatgagc 
      caagacgat ctccaacaa accgccgaa gaagaggaa 
      caggaaaac atcaacgag aagtttatc caggcggag 
      aaacaaaac ggctatccg aaagatagc atttatgaa 
      aatgtcgga caccaaacg aataagact gaattagaa 
      aaatcggac caatatatc gacatgagc ggcaatccg 
      gaagtgaaa gacgaagaa gtcgccgtt tacgaagac 
      atcgacgac gatttgccg ataaaagaa aagccaaat 
      ttaatttta accgacttg tgtgatgtt aagccaaat 
      gatagtagt gaaacgaaa tcggtgaaa gaccgaatg 
      ttgttatca acggatgtg gcaccgtgt ctccttttc 
      acccaaacg gtgacctca ccgatgcta acaccgtcc 
      gaggaaaac atcgatttt ttgaaaggc tttcgccga 
      gaatcgacc gacgaaacg aaagacgaa aacatctac 
      gaaaacacc gagtttctc aaaaatcaa accaacgaa 
      gcgatttac gaaaacctc aaagagaac atctacgaa 
      aacgtgaaa gagttgaac gggaagcag gaagctggt 
      gaggaaata atttaccaa aacgtggag gaattgcgc 
      gataacaac gaggacgaa atcgaagag gaaaaaagc 
      gaagtgatg gacgaaccg gttgatgat gatgttact 
      aatcaaatc gattttgaa agcgaagag agcacacaa 
      gtgaccgag tcgtacgtt gaaacgacg acgttcgaa 
      tcaaactcg tcggaaaaa ggcataaaa aagaaaaaa 
      tcgaaaaag tcgcgaaaa gaggaaaaa gagaatatt 
      tctgttaat ggcactaac actgtttgc gaggactct 
      ttttataac gttaacgtg aaaattttg tgtagaagt 
      ttcggagac ctgactaaa atcagcgac gagtctcaa 
      aagcgaacg cgtactaaa tctttgtcc gatgctagt 
      aacaaaatt gaaaacgtc ttctttgga gtctctgtg 
      cgagccttg ttttttgca tgtgttgtt gtactaact 
      cagagagag aaattctat tgcaatagc ggcgattgt 
      agagcgaaa agtgccgac gggaagtcg agttttagc 
      aaattcgac gctttgcaa aagaaaaat gttctgcat 
      atccgctcg tcggactcg tcgaaagcg caagaaaaa 
      ttcaatggt gcccaaatc gatccgtcg aattgccga 
      tcttgcgga aaagccgtt ttccaaatg gagcaaatc 
      aaagccgag aaggccgtc tggcataaa aattgtttc 
      aggtgtaca gagtgcaat aaacaacta aatgtcgat 
      acatatcaa agtaatgaa ggaagcctt tactgcaaa 
      ccccatttc aaagcctta tttgccccc aaagccgtc 
      gaagacgat gcctctccc aaacctaga aaacccgaa 
      ttgattatt cgggaaagt cagccgatt gaattgcca 
      cctgatgtt gttagagcc agtgacaag cctgacttg 
      ggtttggag gagcttcaa agtctgaac gtgaaagaa 
      cgttttcaa gttttcgaa catcatcag agtgaaaca 
      agtgaagaa cgaataccg tcccatgtc aatgtcaaa 
      cgttcacct tcgattctc tcgaaacta gcaaaattt 
      caggcgaaa ggaatggat atcggggtc gccgatgat 
      tccctgaac ggtatcccg attgaagag agttcctcc 
      gaggaagag gaggaagaa gacgatgga attgagggc 
      gaagatgcc gatttaatc cgagcgaag cgagtgcaa 
      aaagaaaaa ccgtttcat tttacggga atgtcggat 
      gttaagagt cggtgggaa caaggggag caaaccacg 
      tcgcgagac gaacgaaga gaagaaagg aaacaggaa 
      atacaatcg ataagaaac cgactattt atgggaaaa 
      caaggcaaa atgaaggag gcgtaccaa caggccgta 
      atggagtcg gaatcgtgt accaacttg aagaaggaa 
      accccaatc gaagtgtgc gacacaaag tcgctgaaa 
      gagaaattc gaaaagggc gaagtggtc caagaacgc 
      gcccgtgag aaagacgca gaagagaac gaagtttac 
      gagagcgag attagtaaa aaatcgcgg tcgcttttc 
      ctcgaattg gacgcgaac gcgtcaaaa ccgccacaa 
      ataacaccg attacgacg ccgaaaatc gacgtgaag 
      aaagcacgc gataattat ttgtcgaga tcggcatcg 
      gaagatgtt gttaaatca tcggtgaat gtcgtggac 
      gatgtacaa attcgcact tctgatatt cagcaacgt 
      ttcaaattt ttcgaaacg tacaaagaa ccggaaaaa 
      gagaagaaa agttttcgg ataacgccg cctcgcgag 
      ggacaagtc aaggcgcct acgcccgac cgcgaagtc 
      tacagagac ccggaaatc gtccgttcg gaagaaatc 
      atcgaagat tcggtcata gctaaagaa acgcacacc 
      gctacaaaa atgctcaac aaatttcgt caaatggaa 
      gaaaacctc gcaaaagaa ccccaaccc atcggccct 
      aaaccttta aaacggttc actccaccc ccggaaccc 
      actcgtccc gaatcgaac agtgaggct gaaagcgat 
      tccggcgaa gaaaccgaa gaagaggac gaaatcgat 
      cgcggaaaa atcgccgac gaggattta atcgaggca 
      caaaaagcg gccagagcg aagcaattg cgtgccaaa 
      ttcgagcga tgggaagcc aacgaaatt aaaaaggag 
      caaaataat agttcggtg aacattatt gaggaactg 
      ggggaagag cagtcgcaa atcgagtcg accaaaact 
      ttaagggcc cggttcgag tcaatgcgg gagagcagc 
      acggagacg cgacagccg agggtgaaa gtgaaccga 
      tttgtggag cttccgaat gttacggaa aggtgcgaa 
      agttgcaat tcgagagtg tatccttta gagaaaatt 
      tccgtgcac aatcacatc tatcacaag agttgcttc 
      aagtgcatg gaatgtaac tgtgtcttg aggatggat 
      tcgtattcg tacaatcaa ggtctttta tactgcatg 
      ccacatttt aagcgcctt tttatttcc aagggtaat 
      tacgatacc ggatttggg ttaggtcag cataaagaa 
      aagtggaac gccaccata gcttag
    mRNA sequence
    • >TC006112 RA
      atggcattt ttggaccat tttaggaaa ggcgacgac 
      gtgaagcgt ttaatgatt agatatttg cgttcaccg 
      aaaatggcc gaaactgta aaaaagtcc cgaattgta 
      agtgacaac gacgacgat ctcgaggaa gctttgatg 
      gaagaattt aaccagagg ctaaaaaaa tgcatgagc 
      caagacgat ctccaacaa accgccgaa gaagaggaa 
      caggaaaac atcaacgag aagtttatc caggcggag 
      aaacaaaac ggctatccg aaagatagc atttatgaa 
      aatgtcgga caccaaacg aataagact gaattagaa 
      aaatcggac caatatatc gacatgagc ggcaatccg 
      gaagtgaaa gacgaagaa gtcgccgtt tacgaagac 
      atcgacgac gatttgccg ataaaagaa aagccaaat 
      ttaatttta accgacttg tgtgatgtt aagccaaat 
      gatagtagt gaaacgaaa tcggtgaaa gaccgaatg 
      ttgttatca acggatgtg gcaccgtgt ctccttttc 
      acccaaacg gtgacctca ccgatgcta acaccgtcc 
      gaggaaaac atcgatttt ttgaaaggc tttcgccga 
      gaatcgacc gacgaaacg aaagacgaa aacatctac 
      gaaaacacc gagtttctc aaaaatcaa accaacgaa 
      gcgatttac gaaaacctc aaagagaac atctacgaa 
      aacgtgaaa gagttgaac gggaagcag gaagctggt 
      gaggaaata atttaccaa aacgtggag gaattgcgc 
      gataacaac gaggacgaa atcgaagag gaaaaaagc 
      gaagtgatg gacgaaccg gttgatgat gatgttact 
      aatcaaatc gattttgaa agcgaagag agcacacaa 
      gtgaccgag tcgtacgtt gaaacgacg acgttcgaa 
      tcaaactcg tcggaaaaa ggcataaaa aagaaaaaa 
      tcgaaaaag tcgcgaaaa gaggaaaaa gagaatatt 
      tctgttaat ggcactaac actgtttgc gaggactct 
      ttttataac gttaacgtg aaaattttg tgtagaagt 
      ttcggagac ctgactaaa atcagcgac gagtctcaa 
      aagcgaacg cgtactaaa tctttgtcc gatgctagt 
      aacaaaatt gaaaacgtc ttctttgga gtctctgtg 
      cgagccttg ttttttgca tgtgttgtt gtactaact 
      cagagagag aaattctat tgcaatagc ggcgattgt 
      agagcgaaa agtgccgac gggaagtcg agttttagc 
      aaattcgac gctttgcaa aagaaaaat gttctgcat 
      atccgctcg tcggactcg tcgaaagcg caagaaaaa 
      ttcaatggt gcccaaatc gatccgtcg aattgccga 
      tcttgcgga aaagccgtt ttccaaatg gagcaaatc 
      aaagccgag aaggccgtc tggcataaa aattgtttc 
      aggtgtaca gagtgcaat aaacaacta aatgtcgat 
      acatatcaa agtaatgaa ggaagcctt tactgcaaa 
      ccccatttc aaagcctta tttgccccc aaagccgtc 
      gaagacgat gcctctccc aaacctaga aaacccgaa 
      ttgattatt cgggaaagt cagccgatt gaattgcca 
      cctgatgtt gttagagcc agtgacaag cctgacttg 
      ggtttggag gagcttcaa agtctgaac gtgaaagaa 
      cgttttcaa gttttcgaa catcatcag agtgaaaca 
      agtgaagaa cgaataccg tcccatgtc aatgtcaaa 
      cgttcacct tcgattctc tcgaaacta gcaaaattt 
      caggcgaaa ggaatggat atcggggtc gccgatgat 
      tccctgaac ggtatcccg attgaagag agttcctcc 
      gaggaagag gaggaagaa gacgatgga attgagggc 
      gaagatgcc gatttaatc cgagcgaag cgagtgcaa 
      aaagaaaaa ccgtttcat tttacggga atgtcggat 
      gttaagagt cggtgggaa caaggggag caaaccacg 
      tcgcgagac gaacgaaga gaagaaagg aaacaggaa 
      atacaatcg ataagaaac cgactattt atgggaaaa 
      caaggcaaa atgaaggag gcgtaccaa caggccgta 
      atggagtcg gaatcgtgt accaacttg aagaaggaa 
      accccaatc gaagtgtgc gacacaaag tcgctgaaa 
      gagaaattc gaaaagggc gaagtggtc caagaacgc 
      gcccgtgag aaagacgca gaagagaac gaagtttac 
      gagagcgag attagtaaa aaatcgcgg tcgcttttc 
      ctcgaattg gacgcgaac gcgtcaaaa ccgccacaa 
      ataacaccg attacgacg ccgaaaatc gacgtgaag 
      aaagcacgc gataattat ttgtcgaga tcggcatcg 
      gaagatgtt gttaaatca tcggtgaat gtcgtggac 
      gatgtacaa attcgcact tctgatatt cagcaacgt 
      ttcaaattt ttcgaaacg tacaaagaa ccggaaaaa 
      gagaagaaa agttttcgg ataacgccg cctcgcgag 
      ggacaagtc aaggcgcct acgcccgac cgcgaagtc 
      tacagagac ccggaaatc gtccgttcg gaagaaatc 
      atcgaagat tcggtcata gctaaagaa acgcacacc 
      gctacaaaa atgctcaac aaatttcgt caaatggaa 
      gaaaacctc gcaaaagaa ccccaaccc atcggccct 
      aaaccttta aaacggttc actccaccc ccggaaccc 
      actcgtccc gaatcgaac agtgaggct gaaagcgat 
      tccggcgaa gaaaccgaa gaagaggac gaaatcgat 
      cgcggaaaa atcgccgac gaggattta atcgaggca 
      caaaaagcg gccagagcg aagcaattg cgtgccaaa 
      ttcgagcga tgggaagcc aacgaaatt aaaaaggag 
      caaaataat agttcggtg aacattatt gaggaactg 
      ggggaagag cagtcgcaa atcgagtcg accaaaact 
      ttaagggcc cggttcgag tcaatgcgg gagagcagc 
      acggagacg cgacagccg agggtgaaa gtgaaccga 
      tttgtggag cttccgaat gttacggaa aggtgcgaa 
      agttgcaat tcgagagtg tatccttta gagaaaatt 
      tccgtgcac aatcacatc tatcacaag agttgcttc 
      aagtgcatg gaatgtaac tgtgtcttg aggatggat 
      tcgtattcg tacaatcaa ggtctttta tactgcatg 
      ccacatttt aagcgcctt tttatttcc aagggtaat 
      tacgatacc ggatttggg ttaggtcag cataaagaa 
      aagtggaac gccaccata gcttag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY