Status
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Gene information
General information
TC033952
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006098
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2436918..2441019
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033952
    iBeetle screen iB_04066

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    pygopodium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour not present - Number of animals showing the phenotype: 8 (41 dpi)
    Days post injection



    abdominal odoriferous glands partially structure not present - Number of animals showing the phenotype: 8 (41 dpi)
    Days post injection



    abdominal odoriferous glands sometimes content not present - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033952 RA
      MTIKRNFIT LVLCFILYQ AEAGDKEIW PIPTECPKT 
      DPLDYTVHL QHESDCTKF YKCDHGGKV LFDCPAELH 
      FNPVLQVCD WPWRANCTL NDKTTTTTP KPTPTPEPT 
      PPADRDPEC PWPDPMDHT VHLPHETDC TKFYKCDNG 
      KKVEFDCRD GLHFNKELQ VCDWPQNAG CQDNKPSPT 
      PEPETPKPT PSETPEPET PKPTPPEDR DPECPWPDP 
      LDHTVHLPH ETDCTKFYK CDHGKKVEF DCPDGLHFN 
      KELQVCDWP QDAGCESNK PSSTPKPTT EPTPSETPE 
      PETPKPTPP EDRDPECPW PDPLDHTVH LPHETDCTK 
      FYKCDHGKK VEFDCPAGL HFNKELQVC DWPGNAGCE 
      DVKPDPTSK PTPEPTPSE TPEPDTPEP TPPEDKDPE 
      CPWPDPLDH TVHLPHETD CTKFYKCDH GKKVEFDCP 
      DGLHFNKEL QVCDWPQDA GCESNKPSS TPKPTPEPT 
      PSETPEPET PKPTPPEDR DPECPWPDP LDHTVHLPH 
      ETDCTKFYK CDHGKKVEF DCPAGLHFN KELQVCDWP 
      GNAGCEDVK PDPTPKPTP EPTPSETPE PDTPEPTPP 
      EDKDPECPW PDPLDHTVH LPHETDCTK FYKCDHGKK 
      VEFDCPAGL HFNKELQVC DWPGNAGCE DVKPDPTPK 
      PTSEPTPSE TPEPDTPEP TPPEDRDPE CPWPDPLDH 
      TVHLPHETD CTKFYKCDH GKKVEFDCP DGLHFNKEL 
      QVCDWPGNA GCEEVNTDP TEDPTSSTD TPKPTPPED 
      RDPECPWPD PLNYTVHLP HETDCTKFY KCDHGKKVE 
      FDCPDGLHF NKELQVCDW PGNAGCEEV NTDPTDDPT 
      SSTETPKPT PPEDRDPEC PWPDPLNYT VHLPHETDC 
      TKFYKCDHG KKVEFDCPD GLHFNKELQ VCDWPGNAG 
      CEEVNTDPT EDPTSSTDT PKPTPPEDR DPECPWPDP 
      LNYTVHLPH ETDCTKFYK CDHGKKVEF DCPDGLHFN 
      RELQVCDWP GNAGCEEVN TDPTEDPTS STDTPKPTP 
      PEDRDPECP WPDPLNYTV HLPHETDCT KFYKCDHGK 
      KVEFDCPDG LHFNKELQV CDWPGNAGC EEVNTDPTE 
      DPTSSTDTP KPTPPEDRD PECPWPDPL NYTVHLPHE 
      IDCTKFYKC DHGQKVEFE CPDGLHFNP ELEVCDWPE 
      SAGCEDPIP EPCPSDDTD EPEPQPEPT PPSDLDPEC 
      PWPDPLNYT VHLPHEEDC TKFYKCDNG KKVEFDCPD 
      GLHFNPDLE VCDWPENAG CENQSIQCP DTQCQDTTL 
      YLPFLGNPR KYIRCVAGV EVINTCPGG LVFDKNLAT 
      CNLEHLVEP N
    CDS sequence
    • >TC033952 RA
      atgaccatc aaacgtaat tttattacg ttggtcttg 
      tgtttcatt ctttatcaa gcagaagct ggtgataag 
      gaaatatgg ccaataccg accgagtgc cctaaaacc 
      gatccactg gattacact gttcatctc cagcacgaa 
      tcagattgt acgaaattt tataaatgt gatcatggg 
      ggaaaagtt ctctttgat tgtcctgct gagttacat 
      tttaatccg gttttgcaa gtgtgtgat tggccatgg 
      agggccaat tgcacacta aatgacaaa accacaaca 
      actacacca aaaccaact ccgacaccg gaaccaacg 
      ccaccagca gacagagat ccagagtgt ccatggcca 
      gatccaatg gatcacact gttcatcta ccacacgaa 
      acggattgt acgaaattc tacaaatgc gacaatggc 
      aagaaagta gaattcgat tgccgagat gggcttcat 
      ttcaacaaa gaattgcaa gtttgtgat tggccgcaa 
      aacgctggt tgtcaggat aataaacca tcacctact 
      ccagaaccc gaaactccc aaaccaact ccctcagaa 
      actccagag cccgaaact ccaaaacca actccaccc 
      gaagacaga gatccagaa tgtccttgg ccagatcct 
      ttggatcat actgttcat ctaccacac gaaacggat 
      tgtacgaag ttttataaa tgcgaccac ggaaagaaa 
      gttgaattc gattgtcct gatgggcta catttcaat 
      aaggaattg caagtttgt gattggccg caagacgct 
      ggttgtgaa agtaataaa ccatcttct actccaaaa 
      ccaactaca gaaccaact ccctcagaa actccagag 
      cccgaaact ccaaaacca actccacca gaagacaga 
      gatccagag tgtccttgg ccagatcct ttggatcat 
      acagtccat ctaccacat gaaacagac tgtacgaaa 
      ttctataaa tgcgatcat ggaaagaaa gtagaattt 
      gattgtcca gctggacta catttcaac aaagaacta 
      caagtttgt gattggcct ggaaatgct ggttgtgaa 
      gatgtaaaa cctgatcca acttcaaaa ccgactcca 
      gaaccaact ccttcagaa actccagaa cctgatact 
      ccagaacca actccacca gaagacaaa gatccagag 
      tgtccttgg ccagatcca ttggatcat acggttcat 
      ctaccacat gaaacagac tgtacgaaa ttctacaaa 
      tgtgatcat ggcaagaaa gtagaattt gattgtcct 
      gatgggcta catttcaat aaggaattg caagtttgt 
      gattggccg caagacgct ggttgtgaa agtaataaa 
      ccatcttct actccaaaa ccgactcca gaaccaact 
      ccttcagaa acaccagaa cctgaaact ccaaaacca 
      actccacca gaagacaga gatccagag tgtccttgg 
      ccagatcca ttggatcat acagtccat ctaccacat 
      gaaacagac tgtacgaaa ttctataaa tgcgatcat 
      ggaaagaaa gtagaattt gattgtcca gctggacta 
      catttcaac aaagaacta caagtttgt gattggcct 
      ggaaatgct ggttgtgaa gatgtaaaa cctgatcca 
      actccaaaa ccgactcca gaaccaact ccctcagaa 
      actccagaa cctgatact ccagaacca actccacca 
      gaagacaaa gatccagag tgtccttgg ccagatcca 
      ttggatcat acggttcat ctaccacat gaaacagac 
      tgtacgaaa ttctataaa tgcgaccat ggcaagaaa 
      gtagaattt gactgtcca gctgggcta catttcaac 
      aaagaacta caagtttgt gattggcct ggaaatgct 
      ggttgtgag gatgtaaaa cctgatcca actccaaaa 
      ccaacttca gaaccaact ccctcagaa actccagaa 
      cctgatact ccagaacca actccacca gaagacaga 
      gatccagag tgtccatgg ccagatcct ttggatcat 
      acagttcat ctaccacat gaaacagac tgtacaaaa 
      ttctataaa tgcgaccat ggaaagaaa gtagaattt 
      gattgtccc gatggacta catttcaac aaggaattg 
      caagtttgc gattggcca ggaaatgct ggatgtgaa 
      gaagtaaat acagaccct accgaagat ccaacttct 
      tcaacagac actccaaaa ccaactcct ccagaagac 
      agagaccca gaatgccca tggcctgac ccactcaac 
      tacacagtc catttgcca cacgaaaca gactgtaca 
      aaattctac aaatgtgat catggcaag aaagttgaa 
      tttgattgc ccagatggt ctccatttc aacaaggaa 
      ttgcaagtt tgtgactgg ccaggaaac gctggatgt 
      gaagaagta aatacagac cctaccgac gatccaact 
      tcttcaaca gaaactcca aaaccaact cctccagaa 
      gacagagat ccagaatgc ccatggcct gacccactc 
      aactacaca gttcatttg ccacacgaa acagactgc 
      acaaaattc tacaaatgt gatcatggc aagaaagtg 
      gaatttgat tgcccagat ggtctccac ttcaacaag 
      gaattgcaa gtttgcgat tggccagga aacgctggc 
      tgtgaagaa gtaaataca gaccctacc gaagatcca 
      acttcttca acagacact ccaaaacca actcctcca 
      gaagacaga gatccagaa tgcccatgg cctgaccca 
      cttaactac acagtccat ctgccacac gaaacagac 
      tgtacaaaa ttctataaa tgtgatcat ggcaagaaa 
      gttgaattt gattgtcca gatggccta catttcaac 
      agggaattg caagtttgt gactggcca ggaaatgct 
      ggttgtgaa gaagtaaat acagaccct accgaagat 
      ccaacttct tcaacagac actccaaaa ccaactcct 
      ccagaagac agagatcca gaatgccca tggcctgac 
      ccactcaac tacacagtc catttgcca cacgaaaca 
      gactgcaca aaattctac aaatgtgat catggcaag 
      aaagttgaa tttgattgc ccagatggt ctccacttc 
      aacaaggaa ttgcaagtt tgcgattgg ccaggaaac 
      gctggatgt gaagaagta aatacagac cctaccgaa 
      gatccaact tcttcaaca gacactcca aaaccaact 
      cctccagaa gacagagac ccagaatgc ccatggcct 
      gacccactc aactacaca gtccatctc ccacacgaa 
      atcgactgc acaaaattc tacaaatgc gaccatggc 
      caaaaagtc gaattcgaa tgcccagac ggcctccac 
      ttcaaccca gagctagaa gtgtgcgat tggccagaa 
      agcgccggc tgtgaagat ccaatcccc gaaccctgt 
      ccttcagac gacaccgac gaacctgaa ccacaacca 
      gaaccaaca ccaccatca gacctagac ccagaatgc 
      ccatggccc gacccactc aactacacc gtccatcta 
      ccacacgaa gaagactgc acgaaattc tacaaatgt 
      gacaacggc aaaaaagtc gaatttgat tgcccagat 
      ggactacac tttaaccca gacttggaa gtctgtgat 
      tggcccgaa aatgccggt tgtgaaaat caatcaatt 
      caatgcccg gatacacaa tgtcaagac acgacactc 
      tatctaccg tttttgggt aatccgaga aaatacatc 
      cgatgtgtg gcaggtgtc gaagtgatc aatacttgt 
      ccaggaggg ttagtcttc gacaagaat ttggccacg 
      tgcaatttg gaacacttg gttgagcca aattaa
    mRNA sequence
    • >TC033952 RA
      cgtggttag ccttttgtt actataagt gcaaaaaat 
      ttgtagtat agtaataga gaaaacacc gttatgacc 
      atcaaacgt aattttatt acgttggtc ttgtgtttc 
      attctttat caagcagaa gctggtgat aaggaaata 
      tggccaata ccgaccgag tgccctaaa accgatcca 
      ctggattac actgttcat ctccagcac gaatcagat 
      tgtacgaaa ttttataaa tgtgatcat gggggaaaa 
      gttctcttt gattgtcct gctgagtta cattttaat 
      ccggttttg caagtgtgt gattggcca tggagggcc 
      aattgcaca ctaaatgac aaaaccaca acaactaca 
      ccaaaacca actccgaca ccggaacca acgccacca 
      gcagacaga gatccagag tgtccatgg ccagatcca 
      atggatcac actgttcat ctaccacac gaaacggat 
      tgtacgaaa ttctacaaa tgcgacaat ggcaagaaa 
      gtagaattc gattgccga gatgggctt catttcaac 
      aaagaattg caagtttgt gattggccg caaaacgct 
      ggttgtcag gataataaa ccatcacct actccagaa 
      cccgaaact cccaaacca actccctca gaaactcca 
      gagcccgaa actccaaaa ccaactcca cccgaagac 
      agagatcca gaatgtcct tggccagat cctttggat 
      catactgtt catctacca cacgaaacg gattgtacg 
      aagttttat aaatgcgac cacggaaag aaagttgaa 
      ttcgattgt cctgatggg ctacatttc aataaggaa 
      ttgcaagtt tgtgattgg ccgcaagac gctggttgt 
      gaaagtaat aaaccatct tctactcca aaaccaact 
      acagaacca actccctca gaaactcca gagcccgaa 
      actccaaaa ccaactcca ccagaagac agagatcca 
      gagtgtcct tggccagat cctttggat catacagtc 
      catctacca catgaaaca gactgtacg aaattctat 
      aaatgcgat catggaaag aaagtagaa tttgattgt 
      ccagctgga ctacatttc aacaaagaa ctacaagtt 
      tgtgattgg cctggaaat gctggttgt gaagatgta 
      aaacctgat ccaacttca aaaccgact ccagaacca 
      actccttca gaaactcca gaacctgat actccagaa 
      ccaactcca ccagaagac aaagatcca gagtgtcct 
      tggccagat ccattggat catacggtt catctacca 
      catgaaaca gactgtacg aaattctac aaatgtgat 
      catggcaag aaagtagaa tttgattgt cctgatggg 
      ctacatttc aataaggaa ttgcaagtt tgtgattgg 
      ccgcaagac gctggttgt gaaagtaat aaaccatct 
      tctactcca aaaccgact ccagaacca actccttca 
      gaaacacca gaacctgaa actccaaaa ccaactcca 
      ccagaagac agagatcca gagtgtcct tggccagat 
      ccattggat catacagtc catctacca catgaaaca 
      gactgtacg aaattctat aaatgcgat catggaaag 
      aaagtagaa tttgattgt ccagctgga ctacatttc 
      aacaaagaa ctacaagtt tgtgattgg cctggaaat 
      gctggttgt gaagatgta aaacctgat ccaactcca 
      aaaccgact ccagaacca actccctca gaaactcca 
      gaacctgat actccagaa ccaactcca ccagaagac 
      aaagatcca gagtgtcct tggccagat ccattggat 
      catacggtt catctacca catgaaaca gactgtacg 
      aaattctat aaatgcgac catggcaag aaagtagaa 
      tttgactgt ccagctggg ctacatttc aacaaagaa 
      ctacaagtt tgtgattgg cctggaaat gctggttgt 
      gaggatgta aaacctgat ccaactcca aaaccaact 
      tcagaacca actccctca gaaactcca gaacctgat 
      actccagaa ccaactcca ccagaagac agagatcca 
      gagtgtcca tggccagat cctttggat catacagtt 
      catctacca catgaaaca gactgtaca aaattctat 
      aaatgcgac catggaaag aaagtagaa tttgattgt 
      cccgatgga ctacatttc aacaaggaa ttgcaagtt 
      tgcgattgg ccaggaaat gctggatgt gaagaagta 
      aatacagac cctaccgaa gatccaact tcttcaaca 
      gacactcca aaaccaact cctccagaa gacagagac 
      ccagaatgc ccatggcct gacccactc aactacaca 
      gtccatttg ccacacgaa acagactgt acaaaattc 
      tacaaatgt gatcatggc aagaaagtt gaatttgat 
      tgcccagat ggtctccat ttcaacaag gaattgcaa 
      gtttgtgac tggccagga aacgctgga tgtgaagaa 
      gtaaataca gaccctacc gacgatcca acttcttca 
      acagaaact ccaaaacca actcctcca gaagacaga 
      gatccagaa tgcccatgg cctgaccca ctcaactac 
      acagttcat ttgccacac gaaacagac tgcacaaaa 
      ttctacaaa tgtgatcat ggcaagaaa gtggaattt 
      gattgccca gatggtctc cacttcaac aaggaattg 
      caagtttgc gattggcca ggaaacgct ggctgtgaa 
      gaagtaaat acagaccct accgaagat ccaacttct 
      tcaacagac actccaaaa ccaactcct ccagaagac 
      agagatcca gaatgccca tggcctgac ccacttaac 
      tacacagtc catctgcca cacgaaaca gactgtaca 
      aaattctat aaatgtgat catggcaag aaagttgaa 
      tttgattgt ccagatggc ctacatttc aacagggaa 
      ttgcaagtt tgtgactgg ccaggaaat gctggttgt 
      gaagaagta aatacagac cctaccgaa gatccaact 
      tcttcaaca gacactcca aaaccaact cctccagaa 
      gacagagat ccagaatgc ccatggcct gacccactc 
      aactacaca gtccatttg ccacacgaa acagactgc 
      acaaaattc tacaaatgt gatcatggc aagaaagtt 
      gaatttgat tgcccagat ggtctccac ttcaacaag 
      gaattgcaa gtttgcgat tggccagga aacgctgga 
      tgtgaagaa gtaaataca gaccctacc gaagatcca 
      acttcttca acagacact ccaaaacca actcctcca 
      gaagacaga gacccagaa tgcccatgg cctgaccca 
      ctcaactac acagtccat ctcccacac gaaatcgac 
      tgcacaaaa ttctacaaa tgcgaccat ggccaaaaa 
      gtcgaattc gaatgccca gacggcctc cacttcaac 
      ccagagcta gaagtgtgc gattggcca gaaagcgcc 
      ggctgtgaa gatccaatc cccgaaccc tgtccttca 
      gacgacacc gacgaacct gaaccacaa ccagaacca 
      acaccacca tcagaccta gacccagaa tgcccatgg 
      cccgaccca ctcaactac accgtccat ctaccacac 
      gaagaagac tgcacgaaa ttctacaaa tgtgacaac 
      ggcaaaaaa gtcgaattt gattgccca gatggacta 
      cactttaac ccagacttg gaagtctgt gattggccc 
      gaaaatgcc ggttgtgaa aatcaatca attcaatgc 
      ccggataca caatgtcaa gacacgaca ctctatcta 
      ccgtttttg ggtaatccg agaaaatac atccgatgt 
      gtggcaggt gtcgaagtg atcaatact tgtccagga 
      gggttagtc ttcgacaag aatttggcc acgtgcaat 
      ttggaacac ttggttgag ccaaattaa acataattt 
      attatccct tatgtaaaa acaaaaatt aaataaaaa 
      attaaaatt aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY