Status
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Gene information
General information
TC033943
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006085,TC006082,TC006084
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2093526..2152800
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033943
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04065

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles median sometimesstructure duplicated - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033943 RA
      MEVTPHLKN VLKNYQHSA TKSLQGPGL ALKGEEEIK 
      EEEFVPVAL PVQQLPILT TPDTSCSER TETVLEGET 
      ISCFVVGGE KRLCLPQVL NSVLRDFSL PQINHECDQ 
      LQIYCSRCT PEQLTVLKN GGILPSAAA SSGLITKTD 
      AERLCSALL HGHHEVTQI RPRKGLVSF AVYHKCFGK 
      CTGSCFPEL YTGKNAKCI ECAECRGGF SPQQFVCHV 
      HKHFSENRT VHWGFDSAN WRVYLRVAK DQPDQEQCV 
      KYLDEMKEQ FDGKMAFPN VYAGIKRKQ VLASDIIKE 
      HELPLKKQK LLGEYLSPL QSYSLYASL DAMYLNQCI 
      QEFQAANRH LSAFKPVNH HKEPKVYSH LMQMNRYNS 
      DTPVLQNPE RVVLMSESE RFERTFQPN VALAPTIPK 
      KHRYGKGFI KEEFEVKKE EVTNNNNST EVIVKQEVV 
      EEKIPEVAK VVSVVQTTG TLPRYNSEI ELSTDTEDS 
      ASETSEKST DLSKIEELL KGCTARDRV LEVFKGLLK 
      ENERWMKEC RTKDERIKE LEEKVEELE HRPVEETKV 
      ICNGGEVRD HDDNNSSSI IKTNDCEIE GQKTSVIAS 
      LESAVAEPK AIINTAPE
    CDS sequence
    • >TC033943 RA
      atggaagtg acgccgcat ttgaagaac gtgctgaag 
      aactaccag cacagcgcc accaagagt ctgcaaggg 
      ccggggctg gcgctcaag ggcgaggag gagatcaag 
      gaggaggag ttcgtgccg gtggcgttg cccgtgcag 
      cagctgccg atactcacc accccggat acgtcgtgc 
      agtgagcgc acggagacg gtgctcgaa ggagagacg 
      atttcgtgc tttgtggtg ggcggcgag aagcgactc 
      tgtctgccc caggtgctc aactctgtg ctgcgcgac 
      ttttcctta ccccaaatc aaccacgaa tgcgaccag 
      ctgcaaatc tactgttcc cggtgcacc cccgagcaa 
      ctcaccgtt ttgaaaaac ggcggtatt ttaccaagt 
      gccgccgcc agctcaggt ctcatcacc aagacggac 
      gcggagaga ttatgcagc gcccttctg catggccat 
      cacgaggtg acgcagatc aggccacgg aagggctta 
      gtgtcgttc gcggtctac cacaagtgc ttcggcaaa 
      tgcaccggc tcgtgtttt cccgagttg tacacagga 
      aagaatgcc aagtgtatt gagtgtgcg gaatgcagg 
      ggcggtttc tccccacaa cagtttgtc tgtcatgtg 
      cataaacat ttctcggag aatcggaca gtgcattgg 
      gggtttgat tcggctaat tggcgggtg tatttaaga 
      gtggctaag gatcagccg gatcaggag cagtgtgtt 
      aagtatctg gacgagatg aaggagcag tttgatggg 
      aagatggcc tttccgaat gtttatgcg gggattaaa 
      cggaaacag gttctcgct tcggacata ataaaagaa 
      cacgaactg cccctcaag aagcaaaaa cttctcggc 
      gagtatttg agtcctttg caatcgtac agtttgtac 
      gcgtctctg gacgcaatg tatttgaat caatgcatc 
      caggaattc caagctgcc aatcgtcac ttgtcggcc 
      ttcaaaccg gtcaatcat cacaaagaa cccaaagtc 
      tacagccat ctaatgcaa atgaatcgc tacaacagc 
      gacactccc gtgttacaa aaccccgaa cgagttgtc 
      ctcatgtcg gaatcagag cgatttgaa cgcactttc 
      cagccaaat gttgccctt gcgcccaca atacccaag 
      aaacaccgc tatggcaag ggctttatc aaggaggag 
      ttcgaagtg aagaaggag gaagtaacg aataacaac 
      aacagtacg gaggtcata gtgaagcag gaggtggtc 
      gaagagaaa attcccgaa gtggcgaag gttgtgtca 
      gtggttcaa acaacgggg actttaccg agatataac 
      tcagaaatc gaattgtcc accgatact gaagatagt 
      gcgtccgaa acgtcggaa aagtcgacc gatttgagc 
      aaaatcgaa gagttgttg aaaggttgc acagcgcgg 
      gatcgcgtc ttggaggtg tttaaggga cttttgaag 
      gagaacgaa cggtggatg aaggaatgt cgcacgaaa 
      gacgagagg atcaaggag ttggaggag aaggtggag 
      gagttggaa catcggccg gttgaagag actaaagtg 
      atatgcaat gggggggaa gttagagac catgatgat 
      aataacagt tcttccata atcaaaacg aacgattgt 
      gaaattgag ggacaaaaa acgagtgtt attgcgagt 
      ctggaaagt gccgtcgcg gaaccgaaa gcaataata 
      aacactgct ccagaatga
    mRNA sequence
    • >TC033943 RA
      cgccatggc cggagcggc tgcaagcct cctcgttga 
      cgacggcgg tgtccagct tccccaagt gcaggtgtt 
      gggcccaaa gggccacta gtggcagca aattcagcg 
      gaaatcgct gtgttcgtg gaaagcatc gcggtgggt 
      gtgcgcatg tgcggtggc aacagtctg caaaccagg 
      cgagacgac tattgcgac tagcagacc agacatcag 
      tttaggtga acgtctgcg gcgggcggt tgtggttac 
      gtaagtcga tgacattcg gatgacgtg cggtggcgg 
      cagtgtacc accagtgcc atggaagtg acgccgcat 
      ttgaagaac gtgctgaag aactaccag cacagcgcc 
      accaagagt ctgcaaggg ccggggctg gcgctcaag 
      ggcgaggag gagatcaag gaggaggag ttcgtgccg 
      gtggcgttg cccgtgcag cagctgccg atactcacc 
      accccggat acgtcgtgc agtgagcgc acggagacg 
      gtgctcgaa ggagagacg atttcgtgc tttgtggtg 
      ggcggcgag aagcgactc tgtctgccc caggtgctc 
      aactctgtg ctgcgcgac ttttcctta ccccaaatc 
      aaccacgaa tgcgaccag ctgcaaatc tactgttcc 
      cggtgcacc cccgagcaa ctcaccgtt ttgaaaaac 
      ggcggtatt ttaccaagt gccgccgcc agctcaggt 
      ctcatcacc aagacggac gcggagaga ttatgcagc 
      gcccttctg catggccat cacgaggtg acgcagatc 
      aggccacgg aagggctta gtgtcgttc gcggtctac 
      cacaagtgc ttcggcaaa tgcaccggc tcgtgtttt 
      cccgagttg tacacagga aagaatgcc aagtgtatt 
      gagtgtgcg gaatgcagg ggcggtttc tccccacaa 
      cagtttgtc tgtcatgtg cataaacat ttctcggag 
      aatcggaca gtgcattgg gggtttgat tcggctaat 
      tggcgggtg tatttaaga gtggctaag gatcagccg 
      gatcaggag cagtgtgtt aagtatctg gacgagatg 
      aaggagcag tttgatggg aagatggcc tttccgaat 
      gtttatgcg gggattaaa cggaaacag gttctcgct 
      tcggacata ataaaagaa cacgaactg cccctcaag 
      aagcaaaaa cttctcggc gagtatttg agtcctttg 
      caatcgtac agtttgtac gcgtctctg gacgcaatg 
      tatttgaat caatgcatc caggaattc caagctgcc 
      aatcgtcac ttgtcggcc ttcaaaccg gtcaatcat 
      cacaaagaa cccaaagtc tacagccat ctaatgcaa 
      atgaatcgc tacaacagc gacactccc gtgttacaa 
      aaccccgaa cgagttgtc ctcatgtcg gaatcagag 
      cgatttgaa cgcactttc cagccaaat gttgccctt 
      gcgcccaca atacccaag aaacaccgc tatggcaag 
      ggctttatc aaggaggag ttcgaagtg aagaaggag 
      gaagtaacg aataacaac aacagtacg gaggtcata 
      gtgaagcag gaggtggtc gaagagaaa attcccgaa 
      gtggcgaag gttgtgtca gtggttcaa acaacgggg 
      actttaccg agatataac tcagaaatc gaattgtcc 
      accgatact gaagatagt gcgtccgaa acgtcggaa 
      aagtcgacc gatttgagc aaaatcgaa gagttgttg 
      aaaggttgc acagcgcgg gatcgcgtc ttggaggtg 
      tttaaggga cttttgaag gagaacgaa cggtggatg 
      aaggaatgt cgcacgaaa gacgagagg atcaaggag 
      ttggaggag aaggtggag gagttggaa catcggccg 
      gttgaagag actaaagtg atatgcaat gggggggaa 
      gttagagac catgatgat aataacagt tcttccata 
      atcaaaacg aacgattgt gaaattgag ggacaaaaa 
      acgagtgtt attgcgagt ctggaaagt gccgtcgcg 
      gaaccgaaa gcaataata aacactgct ccagaatga 
      tctgtaatt gtgttgtgc cggcgttga acggcactt 
      ttttcgtta ttgtgtctt atttagttg gtaatcgaa 
      gcaatgcgg aagaatagg agagcgccg aaaactcca 
      agagttttg cgttcgccg caaattccg atttgtaca 
      tagtcgtac tctagacca gtcaaaacg aaaatttga 
      ctttaaagc gaactaatt ttaggaaaa gttaaaaaa 
      aattaaaac gaactgcta gttgtgaaa ctacatttt 
      tgattggtt gtctttaac caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY