| General information | |||
| TC006063 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 2158809..2221385 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006063 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04063 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
| Comment: Very few larvae also show missing head appendages and /or irregular shape/orientation. |
urogomphi size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
pygopodium size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC006063 RA
MDNIANNNL VSNLPEERG WEYKQICVT RVPGYGFGI AVSGGRDNP HFASGDPSI AVSDVLASG PAERILQVN DRIISVNGI SLQNVDYAT AVQVLRDSG DTVTLVIKR RTTQHQHSH SLSASYTGA PLAALGVNP AATKVTLTK NSKKEDFGI VLGCKLFVK EISSKARDQ LLNTNQVLV EGDFITKIN NTNCNDLMS LKEAKKIID GTKDKLTIS IARDFTSNI SHQTQSSIS TTGAVNNFY KGDDFLTSQ SYSNQNLYV QPPTRNPNL NTSGGFEDK LDPNSGPNS LVDDKSNLA PRGRTRGPL SEANLNQLD NRNSLGGYG RSKGRDEPP RPPPPRAED YYSSRRQIY DDDPIIQKK QPIPDARFV TFHKEGSVG IRLTGGNFV GIFVTAVQP GSPASLQGL QPGDKILKV NDMDMTGVT REEAVLFLL SLQDRIELI VQYCKEEYD NIVASQKGD SFYIKTHFH YDNPAKGEM SFRSGDIFH VIDTLYNGV VGSWQVYRI GRNNQEVQK GIIPNKARA EELATAQFN ATKKEMSAN ESRGSFFRR RRSSNRRSK SLGKEHWDD ILFSDTVSK FPAYERVLL KHPGFTRPV ILFGPIADV AREKLLKDF PDKFISPQL DSTNEENTK SQKTSNIIR LSAIRDVIS TGKHALLDI TPNAVERLN YAQIYPIVV FFKAESKIV IKQLRQGLP KSAHKSSKK LLEQCQKLD KVWSHIFSA TITMNDNTD NWYRKVRDI IDKQQSGAL WMSESKPEE SLSDDFLFP MSSLRLSYA SSPESDLEH SPGPPVSSN SPTAGRLTK ASSDPSVVM PDSSTTDVL PPYQPNYDS RYGFNNNQS NDQVNLTKE PNYLSNQDI PGSLDQYGK SGLYNTQMD TRTDFGNVQ HKRQVSSLH HGYNHPKSH SINEQSPRR SPKEGVYGQ VPDLPPRID RAIKPISLL TTPSKIPNG RSAHERLFG TKPGQALDN PEQNNNDNN NFSNKLGSL ERSQNSKSD SLSSYDSFN KQTNNRIGP NAHDDLKTT LSKIEPGSV QNSPSKYNS GNRWDQNPH RHNSKLDLK YGLPQNDHN DNSPRNSRE LDKESRPME NMSPRGSVE RDIYRYSRS PAKKGHVET KTDYGKYSR NNSSAQDYA RSSHQDLSQ SHLSVHQVP PHHHSPMKS PPVHHNGFD NGGHMGPPN GNGYKPVPP PKPKNYKPP YKGQQGYGE GMVQPPMPT YQHGKSHSN PVDQRAQNM NYYYNMPQN NSWHTETAV RFNSEMHGH YDMAPPYET RRHPSISYA SRSEITPIA QHDNPYGMD GGDMLMDAR PPERSNIVD LQGSREQRG SAFELYRKP VHHNLRVQD DGALFGSQP NIIQKLNTN NSPKVTPKL TKTQSFKHQ RSEGGGLSA AKIFRKFSF KSRSREGTP KMPKKLSVK PKDDRMRPS KRDITSAII DHRGGTMIN EYWGVSLEI PENAIPIGE QKEIYFVIS DPRLCDNNV PPLDLENGE AMLSPIVMC GPQGTEFLK PVILNIPHY ANTLPSLGI SLKATDSEQ DVHTDWDNI LLPSNHAAN SVAVKVDHF
- >TC006063 RA
atggataac atagccaac aacaatttg gtttcgaat ttacctgag gaacgcggc tgggagtac aaacaaata tgcgtaacc cgagttccg ggctatggg ttcggaatt gccgtctcc ggaggcaga gacaatccg catttcgcc tccggtgac cccagcatc gctgtcagt gatgttcta gcttctggt ccggccgaa agaattttg caggtcaac gacaggatt atttccgtg aatggaatc tcccttcag aatgttgat tacgccacg gcggttcaa gtgctgagg gattccgga gatacagtg accttagtg attaagagg aggacgact cagcatcag catagtcat agtctcagt gcgtcctac actggagca ccacttgct gccttggga gttaatcct gcggcgaca aaggtcacc cttaccaaa aattccaag aaagaagat ttcggcata gtcctgggc tgcaagctc ttcgttaaa gagatctcg tcaaaagcc cgcgaccaa ctcctcaac accaaccaa gtccttgtc gaaggcgac tttatcaca aaaatcaac aacacgaac tgtaacgac ttaatgtcg ctcaaagag gctaaaaaa atcatcgac ggtacaaaa gacaaacta acaatctca atagcacgt gactttaca tcgaatatt tctcatcag acacaaagt tcaataagc accactggc gctgttaac aatttttac aaaggtgat gacttttta acatcgcag agttattca aatcagaat ttgtacgtg caaccaccg accagaaac cccaacctc aatacgagt ggggggttt gaggataag ttggaccca aatagtgga ccgaattct ttggttgat gataagagc aatttggca ccgagggga agaacacgg gggccctta tcagaggct aatttgaac cagttggat aataggaat agtttaggg gggtatggc aggtccaag gggagagat gagccgcct aggcctccg cccccacga gcagaagat tattacagt tctcgccga caaatctac gatgatgat ccaattatc cagaagaag caaccgata cccgacgct cgattcgtg acattccac aaagagggt tctgtggga attcgtcta actggtggc aactttgtc ggaattttc gtaacagcc gtgcaacca ggaagtcca gcatccctt caaggtttg caacctggt gataaaatc ctcaaagtg aatgatatg gatatgact ggcgtaacg cgagaagaa gccgttctt ttcctctta agtctccaa gatcgcatc gaactaatt gttcaatac tgcaaagaa gaatacgac aatatagtc gcgtcccaa aaaggcgac agtttttac atcaagacc cactttcat tacgacaat ccggcaaaa ggtgaaatg tctttccgt tccggagac attttccac gtcatcgat acgctgtat aacggagtc gtgggctcg tggcaagtg tacagaatt ggacgcaac aatcaagaa gtgcaaaag ggaattatt ccgaataaa gctagagcc gaggaactg gcaacggct caattcaac gcaacgaaa aaagaaatg tcggctaat gaatcaaga gggagcttc ttcaggaga cgaaggagt tcgaacaga cggtcgaaa agtctggga aaagagcat tgggatgat attttgttt tccgacact gtttccaaa tttcctgct tatgaacgc gttttgctc aaacatcct gggtttaca agacctgtt attctcttt gggccgatt gctgacgtc gcaagggag aaactactc aaggatttt ccggataaa ttcatatca cctcaactc gattcaaca aatgaggaa aacacaaaa tcgcagaaa acttcaaac atcatacga ttatcagcg attcgtgac gtcatttca actgggaaa cacgccctt ttggacata actcccaac gcggttgaa cgcttgaat tatgcccaa atttaccca attgtagtg tttttcaaa gctgagagc aagattgtg atcaagcag ttgcgtcag ggcttgccc aagtctgcg cacaaaagt tcgaaaaaa ttgctagaa caatgccaa aaactggac aaagtgtgg agccacatt ttcagtgct accataaca atgaacgac aatacggat aactggtat cgcaaagtt cgtgatatt attgataag caacaaagt ggcgccctc tggatgagc gaaagcaag cccgaagaa tcactctca gacgatttc ctgttccca atgtcatct ttgcgacta tcatacgct agtagtcct gaaagtgac ttggaacac agtcctggc cctccagtc tcttcaaat tcaccaaca gctggacgc ttaacaaaa gccagttct gatccaagt gttgtaatg cctgatagt agcaccaca gatgtttta ccgccatat cagccaaac tacgactct aggtatggt tttaacaac aatcaatcg aatgaccaa gttaatttg accaaagaa ccgaactat ttgtccaat caagatatt cctggtagt ttggaccag tatggcaaa agtgggtta tataatact caaatggac acgagaact gattttggg aatgtgcaa cataagaga caagttagt agtcttcat catgggtat aaccatccc aagagtcat agcataaac gagcaaagt ccgcgaagg agtcccaag gaaggggtt tatgggcaa gtccccgat ttaccaccg cgcattgac cgcgcaatc aaacccatc agtttgtta accacaccc agcaaaata cccaacggc cgttcggca catgaaaga cttttcggt accaaaccg ggtcaagct ctggataat ccggaacaa aacaacaac gataataac aatttcagc aacaagttg ggctcatta gaacgaagt cagaactca aagtcggat tctctttca agttacgat tctttcaac aaacagacg aataatcga atcggtccg aacgctcac gacgatctc aaaacaacc ctgtccaaa atcgagcct ggctctgtc cagaatagt ccgtccaaa tacaattct ggcaatagg tgggaccaa aacccacac agacacaac tcgaagctg gatttgaaa tatggtttg cctcaaaat gaccacaat gacaatagt ccgcgcaat tctcgcgag ttggataag gaatcgcgt ccaatggaa aatatgagt ccgagagga tctgtcgaa cgtgatatt tatcgatac tcgaggtct cctgcgaaa aaggggcac gtggaaacc aaaacagat tatggtaaa tacagtcgg aataacagt tccgcacaa gattatgcg aggagttcg catcaggat ttgagtcag tcgcatttg agtgtgcac caagtgcct ccacatcac cactcgccc atgaagagt ccaccggtg caccacaat gggtttgat aatggaggt catatggga cctcctaac ggtaatggg tataagccg gtgccgcct ccgaaaccg aaaaattac aaacctcca tataaaggg caacagggt tatggggaa gggatggtg caaccacct atgccgaca taccaacat gggaagagt cacagtaat ccagtggac caaagggcc caaaacatg aactactat tacaacatg ccacaaaac aacagttgg cacactgaa acggccgtc cgatttaat tccgaaatg cacggtcat tacgacatg gctcctcca tacgaaacg aggagacac ccaagcata tcatacgcc tccaggagt gaaataaca cccattgcc caacacgat aacccatat gggatggat ggaggcgat atgttgatg gatgcacgg cctccggaa cgcagcaat attgtcgat ttgcaaggg tcaagggaa caaagagga tcggcattt gagttgtac agaaaacct gtacatcat aatttaagg gttcaggat gatggcgct ttgttcgga tcacagcct aacataatc caaaaacta aacactaat aactcgcct aaagtaacg ccaaaacta accaaaacg caatcgttc aaacaccaa cgctccgaa ggcggcggt ctcagcgca gcgaaaatc ttccgaaag ttttcgttt aaatcgaga agtagggaa ggcacaccc aagatgccg aaaaaatta tcagtgaaa cccaaagat gaccgaatg cgaccgagc aaacgcgat atcacttcg gccatcatc gaccaccga ggtggcacg atgattaac gagtattgg ggcgtttcg ctggaaata cccgaaaat gcgatcccc attggggaa caaaaagaa atctatttt gttatttcc gatccgagg ttgtgtgat aacaacgtg ccgccgctg gatttggaa aacggtgaa gcaatgtta tcgccaatt gtcatgtgc ggaccgcaa ggaactgag tttttgaag cctgtgatc ctcaatatt ccgcattat gccaacaca ttgcccagt ctcgggatc agtttgaaa gcgacagac tccgaacaa gatgtgcac acagactgg gacaacatt ttactgcct agcaatcac gccgccaac agtgtggcc gttaaagtc gatcacttt taa
- >TC006063 RA
atgtaggta ccattcgca attaaaata agtgattgg gagtgggtg tacctattg ttctggcac attttgcgt tttccccca atcgattac caacaaccc acaaggtca aatcgctta agacgtttt caaatgcga cagattcta gttgtcgcg ttgttggtc cacagcctt caaccggta acagaaaga gaagggacg gtggacttt cttaagtca gttagttta gttgctaga cgcattccg aaccgacgc tgacttgtc cccttatat atttttttc tctaaactg gaataaatg tgataacat ttatcaatt attacaaag tgtaaaata aggatttct ccccatttc gtgcgagtt ttcagtgaa tttttggga ttgacaacg cgtgtcaag tttaaatgt ccatgttgt tggtgttgt gtctgtgtt gtgtggtgc tgaatttgt tgcacagaa atggataac atagccaac aacaatttg gtttcgaat ttacctgag gaacgcggc tgggagtac aaacaaata tgcgtaacc cgagttccg ggctatggg ttcggaatt gccgtctcc ggaggcaga gacaatccg catttcgcc tccggtgac cccagcatc gctgtcagt gatgttcta gcttctggt ccggccgaa agaattttg caggtcaac gacaggatt atttccgtg aatggaatc tcccttcag aatgttgat tacgccacg gcggttcaa gtgctgagg gattccgga gatacagtg accttagtg attaagagg aggacgact cagcatcag catagtcat agtctcagt gcgtcctac actggagca ccacttgct gccttggga gttaatcct gcggcgaca aaggtcacc cttaccaaa aattccaag aaagaagat ttcggcata gtcctgggc tgcaagctc ttcgttaaa gagatctcg tcaaaagcc cgcgaccaa ctcctcaac accaaccaa gtccttgtc gaaggcgac tttatcaca aaaatcaac aacacgaac tgtaacgac ttaatgtcg ctcaaagag gctaaaaaa atcatcgac ggtacaaaa gacaaacta acaatctca atagcacgt gactttaca tcgaatatt tctcatcag acacaaagt tcaataagc accactggc gctgttaac aatttttac aaaggtgat gacttttta acatcgcag agttattca aatcagaat ttgtacgtg caaccaccg accagaaac cccaacctc aatacgagt ggggggttt gaggataag ttggaccca aatagtgga ccgaattct ttggttgat gataagagc aatttggca ccgagggga agaacacgg gggccctta tcagaggct aatttgaac cagttggat aataggaat agtttaggg gggtatggc aggtccaag gggagagat gagccgcct aggcctccg cccccacga gcagaagat tattacagt tctcgccga caaatctac gatgatgat ccaattatc cagaagaag caaccgata cccgacgct cgattcgtg acattccac aaagagggt tctgtggga attcgtcta actggtggc aactttgtc ggaattttc gtaacagcc gtgcaacca ggaagtcca gcatccctt caaggtttg caacctggt gataaaatc ctcaaagtg aatgatatg gatatgact ggcgtaacg cgagaagaa gccgttctt ttcctctta agtctccaa gatcgcatc gaactaatt gttcaatac tgcaaagaa gaatacgac aatatagtc gcgtcccaa aaaggcgac agtttttac atcaagacc cactttcat tacgacaat ccggcaaaa ggtgaaatg tctttccgt tccggagac attttccac gtcatcgat acgctgtat aacggagtc gtgggctcg tggcaagtg tacagaatt ggacgcaac aatcaagaa gtgcaaaag ggaattatt ccgaataaa gctagagcc gaggaactg gcaacggct caattcaac gcaacgaaa aaagaaatg tcggctaat gaatcaaga gggagcttc ttcaggaga cgaaggagt tcgaacaga cggtcgaaa agtctggga aaagagcat tgggatgat attttgttt tccgacact gtttccaaa tttcctgct tatgaacgc gttttgctc aaacatcct gggtttaca agacctgtt attctcttt gggccgatt gctgacgtc gcaagggag aaactactc aaggatttt ccggataaa ttcatatca cctcaactc gattcaaca aatgaggaa aacacaaaa tcgcagaaa acttcaaac atcatacga ttatcagcg attcgtgac gtcatttca actgggaaa cacgccctt ttggacata actcccaac gcggttgaa cgcttgaat tatgcccaa atttaccca attgtagtg tttttcaaa gctgagagc aagattgtg atcaagcag ttgcgtcag ggcttgccc aagtctgcg cacaaaagt tcgaaaaaa ttgctagaa caatgccaa aaactggac aaagtgtgg agccacatt ttcagtgct accataaca atgaacgac aatacggat aactggtat cgcaaagtt cgtgatatt attgataag caacaaagt ggcgccctc tggatgagc gaaagcaag cccgaagaa tcactctca gacgatttc ctgttccca atgtcatct ttgcgacta tcatacgct agtagtcct gaaagtgac ttggaacac agtcctggc cctccagtc tcttcaaat tcaccaaca gctggacgc ttaacaaaa gccagttct gatccaagt gttgtaatg cctgatagt agcaccaca gatgtttta ccgccatat cagccaaac tacgactct aggtatggt tttaacaac aatcaatcg aatgaccaa gttaatttg accaaagaa ccgaactat ttgtccaat caagatatt cctggtagt ttggaccag tatggcaaa agtgggtta tataatact caaatggac acgagaact gattttggg aatgtgcaa cataagaga caagttagt agtcttcat catgggtat aaccatccc aagagtcat agcataaac gagcaaagt ccgcgaagg agtcccaag gaaggggtt tatgggcaa gtccccgat ttaccaccg cgcattgac cgcgcaatc aaacccatc agtttgtta accacaccc agcaaaata cccaacggc cgttcggca catgaaaga cttttcggt accaaaccg ggtcaagct ctggataat ccggaacaa aacaacaac gataataac aatttcagc aacaagttg ggctcatta gaacgaagt cagaactca aagtcggat tctctttca agttacgat tctttcaac aaacagacg aataatcga atcggtccg aacgctcac gacgatctc aaaacaacc ctgtccaaa atcgagcct ggctctgtc cagaatagt ccgtccaaa tacaattct ggcaatagg tgggaccaa aacccacac agacacaac tcgaagctg gatttgaaa tatggtttg cctcaaaat gaccacaat gacaatagt ccgcgcaat tctcgcgag ttggataag gaatcgcgt ccaatggaa aatatgagt ccgagagga tctgtcgaa cgtgatatt tatcgatac tcgaggtct cctgcgaaa aaggggcac gtggaaacc aaaacagat tatggtaaa tacagtcgg aataacagt tccgcacaa gattatgcg aggagttcg catcaggat ttgagtcag tcgcatttg agtgtgcac caagtgcct ccacatcac cactcgccc atgaagagt ccaccggtg caccacaat gggtttgat aatggaggt catatggga cctcctaac ggtaatggg tataagccg gtgccgcct ccgaaaccg aaaaattac aaacctcca tataaaggg caacagggt tatggggaa gggatggtg caaccacct atgccgaca taccaacat gggaagagt cacagtaat ccagtggac caaagggcc caaaacatg aactactat tacaacatg ccacaaaac aacagttgg cacactgaa acggccgtc cgatttaat tccgaaatg cacggtcat tacgacatg gctcctcca tacgaaacg aggagacac ccaagcata tcatacgcc tccaggagt gaaataaca cccattgcc caacacgat aacccatat gggatggat ggaggcgat atgttgatg gatgcacgg cctccggaa cgcagcaat attgtcgat ttgcaaggg tcaagggaa caaagagga tcggcattt gagttgtac agaaaacct gtacatcat aatttaagg gttcaggat gatggcgct ttgttcgga tcacagcct aacataatc caaaaacta aacactaat aactcgcct aaagtaacg ccaaaacta accaaaacg caatcgttc aaacaccaa cgctccgaa ggcggcggt ctcagcgca gcgaaaatc ttccgaaag ttttcgttt aaatcgaga agtagggaa ggcacaccc aagatgccg aaaaaatta tcagtgaaa cccaaagat gaccgaatg cgaccgagc aaacgcgat atcacttcg gccatcatc gaccaccga ggtggcacg atgattaac gagtattgg ggcgtttcg ctggaaata cccgaaaat gcgatcccc attggggaa caaaaagaa atctatttt gttatttcc gatccgagg ttgtgtgat aacaacgtg ccgccgctg gatttggaa aacggtgaa gcaatgtta tcgccaatt gtcatgtgc ggaccgcaa ggaactgag tttttgaag cctgtgatc ctcaatatt ccgcattat gccaacaca ttgcccagt ctcgggatc agtttgaaa gcgacagac tccgaacaa gatgtgcac acagactgg gacaacatt ttactgcct agcaatcac gccgccaac agtgtggcc gttaaagtc gatcacttt taaatttat tatttaatc agcgacaaa tttgctcaa gtttgccat tttttatta ctatttcgc tctcattat atttttatt ttaaatgta tttctttta ttttttaga ctgatgtaa ctgcaactt gtttgtaat tttatttgt aaataaacg aaatttatt tta
