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Gene information
General information
TC006063 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 1853387..1915963
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006063
    iBeetle screen iB_04063
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Very few larvae also show missing head appendages and /or irregular shape/orientation.
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    pygopodium size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006063 RA
      MDNIANNNL VSNLPEERG WEYKQICVT RVPGYGFGI 
      AVSGGRDNP HFASGDPSI AVSDVLASG PAERILQVN 
      DRIISVNGI SLQNVDYAT AVQVLRDSG DTVTLVIKR 
      RTTQHQHSH SLSASYTGA PLAALGVNP AATKVTLTK 
      NSKKEDFGI VLGCKLFVK EISSKARDQ LLNTNQVLV 
      EGDFITKIN NTNCNDLMS LKEAKKIID GTKDKLTIS 
      IARDFTSNI SHQTQSSIS TTGAVNNFY KGDDFLTSQ 
      SYSNQNLYV QPPTRNPNL NTSGGFEDK LDPNSGPNS 
      LVDDKSNLA PRGRTRGPL SEANLNQLD NRNSLGGYG 
      RSKGRDEPP RPPPPRAED YYSSRRQIY DDDPIIQKK 
      QPIPDARFV TFHKEGSVG IRLTGGNFV GIFVTAVQP 
      GSPASLQGL QPGDKILKV NDMDMTGVT REEAVLFLL 
      SLQDRIELI VQYCKEEYD NIVASQKGD SFYIKTHFH 
      YDNPAKGEM SFRSGDIFH VIDTLYNGV VGSWQVYRI 
      GRNNQEVQK GIIPNKARA EELATAQFN ATKKEMSAN 
      ESRGSFFRR RRSSNRRSK SLGKEHWDD ILFSDTVSK 
      FPAYERVLL KHPGFTRPV ILFGPIADV AREKLLKDF 
      PDKFISPQL DSTNEENTK SQKTSNIIR LSAIRDVIS 
      TGKHALLDI TPNAVERLN YAQIYPIVV FFKAESKIV 
      IKQLRQGLP KSAHKSSKK LLEQCQKLD KVWSHIFSA 
      TITMNDNTD NWYRKVRDI IDKQQSGAL WMSESKPEE 
      SLSDDFLFP MSSLRLSYA SSPESDLEH SPGPPVSSN 
      SPTAGRLTK ASSDPSVVM PDSSTTDVL PPYQPNYDS 
      RYGFNNNQS NDQVNLTKE PNYLSNQDI PGSLDQYGK 
      SGLYNTQMD TRTDFGNVQ HKRQVSSLH HGYNHPKSH 
      SINEQSPRR SPKEGVYGQ VPDLPPRID RAIKPISLL 
      TTPSKIPNG RSAHERLFG TKPGQALDN PEQNNNDNN 
      NFSNKLGSL ERSQNSKSD SLSSYDSFN KQTNNRIGP 
      NAHDDLKTT LSKIEPGSV QNSPSKYNS GNRWDQNPH 
      RHNSKLDLK YGLPQNDHN DNSPRNSRE LDKESRPME 
      NMSPRGSVE RDIYRYSRS PAKKGHVET KTDYGKYSR 
      NNSSAQDYA RSSHQDLSQ SHLSVHQVP PHHHSPMKS 
      PPVHHNGFD NGGHMGPPN GNGYKPVPP PKPKNYKPP 
      YKGQQGYGE GMVQPPMPT YQHGKSHSN PVDQRAQNM 
      NYYYNMPQN NSWHTETAV RFNSEMHGH YDMAPPYET 
      RRHPSISYA SRSEITPIA QHDNPYGMD GGDMLMDAR 
      PPERSNIVD LQGSREQRG SAFELYRKP VHHNLRVQD 
      DGALFGSQP NIIQKLNTN NSPKVTPKL TKTQSFKHQ 
      RSEGGGLSA AKIFRKFSF KSRSREGTP KMPKKLSVK 
      PKDDRMRPS KRDITSAII DHRGGTMIN EYWGVSLEI 
      PENAIPIGE QKEIYFVIS DPRLCDNNV PPLDLENGE 
      AMLSPIVMC GPQGTEFLK PVILNIPHY ANTLPSLGI 
      SLKATDSEQ DVHTDWDNI LLPSNHAAN SVAVKVDHF
    CDS sequence
    • >TC006063 RA
      atggataac atagccaac aacaatttg gtttcgaat 
      ttacctgag gaacgcggc tgggagtac aaacaaata 
      tgcgtaacc cgagttccg ggctatggg ttcggaatt 
      gccgtctcc ggaggcaga gacaatccg catttcgcc 
      tccggtgac cccagcatc gctgtcagt gatgttcta 
      gcttctggt ccggccgaa agaattttg caggtcaac 
      gacaggatt atttccgtg aatggaatc tcccttcag 
      aatgttgat tacgccacg gcggttcaa gtgctgagg 
      gattccgga gatacagtg accttagtg attaagagg 
      aggacgact cagcatcag catagtcat agtctcagt 
      gcgtcctac actggagca ccacttgct gccttggga 
      gttaatcct gcggcgaca aaggtcacc cttaccaaa 
      aattccaag aaagaagat ttcggcata gtcctgggc 
      tgcaagctc ttcgttaaa gagatctcg tcaaaagcc 
      cgcgaccaa ctcctcaac accaaccaa gtccttgtc 
      gaaggcgac tttatcaca aaaatcaac aacacgaac 
      tgtaacgac ttaatgtcg ctcaaagag gctaaaaaa 
      atcatcgac ggtacaaaa gacaaacta acaatctca 
      atagcacgt gactttaca tcgaatatt tctcatcag 
      acacaaagt tcaataagc accactggc gctgttaac 
      aatttttac aaaggtgat gacttttta acatcgcag 
      agttattca aatcagaat ttgtacgtg caaccaccg 
      accagaaac cccaacctc aatacgagt ggggggttt 
      gaggataag ttggaccca aatagtgga ccgaattct 
      ttggttgat gataagagc aatttggca ccgagggga 
      agaacacgg gggccctta tcagaggct aatttgaac 
      cagttggat aataggaat agtttaggg gggtatggc 
      aggtccaag gggagagat gagccgcct aggcctccg 
      cccccacga gcagaagat tattacagt tctcgccga 
      caaatctac gatgatgat ccaattatc cagaagaag 
      caaccgata cccgacgct cgattcgtg acattccac 
      aaagagggt tctgtggga attcgtcta actggtggc 
      aactttgtc ggaattttc gtaacagcc gtgcaacca 
      ggaagtcca gcatccctt caaggtttg caacctggt 
      gataaaatc ctcaaagtg aatgatatg gatatgact 
      ggcgtaacg cgagaagaa gccgttctt ttcctctta 
      agtctccaa gatcgcatc gaactaatt gttcaatac 
      tgcaaagaa gaatacgac aatatagtc gcgtcccaa 
      aaaggcgac agtttttac atcaagacc cactttcat 
      tacgacaat ccggcaaaa ggtgaaatg tctttccgt 
      tccggagac attttccac gtcatcgat acgctgtat 
      aacggagtc gtgggctcg tggcaagtg tacagaatt 
      ggacgcaac aatcaagaa gtgcaaaag ggaattatt 
      ccgaataaa gctagagcc gaggaactg gcaacggct 
      caattcaac gcaacgaaa aaagaaatg tcggctaat 
      gaatcaaga gggagcttc ttcaggaga cgaaggagt 
      tcgaacaga cggtcgaaa agtctggga aaagagcat 
      tgggatgat attttgttt tccgacact gtttccaaa 
      tttcctgct tatgaacgc gttttgctc aaacatcct 
      gggtttaca agacctgtt attctcttt gggccgatt 
      gctgacgtc gcaagggag aaactactc aaggatttt 
      ccggataaa ttcatatca cctcaactc gattcaaca 
      aatgaggaa aacacaaaa tcgcagaaa acttcaaac 
      atcatacga ttatcagcg attcgtgac gtcatttca 
      actgggaaa cacgccctt ttggacata actcccaac 
      gcggttgaa cgcttgaat tatgcccaa atttaccca 
      attgtagtg tttttcaaa gctgagagc aagattgtg 
      atcaagcag ttgcgtcag ggcttgccc aagtctgcg 
      cacaaaagt tcgaaaaaa ttgctagaa caatgccaa 
      aaactggac aaagtgtgg agccacatt ttcagtgct 
      accataaca atgaacgac aatacggat aactggtat 
      cgcaaagtt cgtgatatt attgataag caacaaagt 
      ggcgccctc tggatgagc gaaagcaag cccgaagaa 
      tcactctca gacgatttc ctgttccca atgtcatct 
      ttgcgacta tcatacgct agtagtcct gaaagtgac 
      ttggaacac agtcctggc cctccagtc tcttcaaat 
      tcaccaaca gctggacgc ttaacaaaa gccagttct 
      gatccaagt gttgtaatg cctgatagt agcaccaca 
      gatgtttta ccgccatat cagccaaac tacgactct 
      aggtatggt tttaacaac aatcaatcg aatgaccaa 
      gttaatttg accaaagaa ccgaactat ttgtccaat 
      caagatatt cctggtagt ttggaccag tatggcaaa 
      agtgggtta tataatact caaatggac acgagaact 
      gattttggg aatgtgcaa cataagaga caagttagt 
      agtcttcat catgggtat aaccatccc aagagtcat 
      agcataaac gagcaaagt ccgcgaagg agtcccaag 
      gaaggggtt tatgggcaa gtccccgat ttaccaccg 
      cgcattgac cgcgcaatc aaacccatc agtttgtta 
      accacaccc agcaaaata cccaacggc cgttcggca 
      catgaaaga cttttcggt accaaaccg ggtcaagct 
      ctggataat ccggaacaa aacaacaac gataataac 
      aatttcagc aacaagttg ggctcatta gaacgaagt 
      cagaactca aagtcggat tctctttca agttacgat 
      tctttcaac aaacagacg aataatcga atcggtccg 
      aacgctcac gacgatctc aaaacaacc ctgtccaaa 
      atcgagcct ggctctgtc cagaatagt ccgtccaaa 
      tacaattct ggcaatagg tgggaccaa aacccacac 
      agacacaac tcgaagctg gatttgaaa tatggtttg 
      cctcaaaat gaccacaat gacaatagt ccgcgcaat 
      tctcgcgag ttggataag gaatcgcgt ccaatggaa 
      aatatgagt ccgagagga tctgtcgaa cgtgatatt 
      tatcgatac tcgaggtct cctgcgaaa aaggggcac 
      gtggaaacc aaaacagat tatggtaaa tacagtcgg 
      aataacagt tccgcacaa gattatgcg aggagttcg 
      catcaggat ttgagtcag tcgcatttg agtgtgcac 
      caagtgcct ccacatcac cactcgccc atgaagagt 
      ccaccggtg caccacaat gggtttgat aatggaggt 
      catatggga cctcctaac ggtaatggg tataagccg 
      gtgccgcct ccgaaaccg aaaaattac aaacctcca 
      tataaaggg caacagggt tatggggaa gggatggtg 
      caaccacct atgccgaca taccaacat gggaagagt 
      cacagtaat ccagtggac caaagggcc caaaacatg 
      aactactat tacaacatg ccacaaaac aacagttgg 
      cacactgaa acggccgtc cgatttaat tccgaaatg 
      cacggtcat tacgacatg gctcctcca tacgaaacg 
      aggagacac ccaagcata tcatacgcc tccaggagt 
      gaaataaca cccattgcc caacacgat aacccatat 
      gggatggat ggaggcgat atgttgatg gatgcacgg 
      cctccggaa cgcagcaat attgtcgat ttgcaaggg 
      tcaagggaa caaagagga tcggcattt gagttgtac 
      agaaaacct gtacatcat aatttaagg gttcaggat 
      gatggcgct ttgttcgga tcacagcct aacataatc 
      caaaaacta aacactaat aactcgcct aaagtaacg 
      ccaaaacta accaaaacg caatcgttc aaacaccaa 
      cgctccgaa ggcggcggt ctcagcgca gcgaaaatc 
      ttccgaaag ttttcgttt aaatcgaga agtagggaa 
      ggcacaccc aagatgccg aaaaaatta tcagtgaaa 
      cccaaagat gaccgaatg cgaccgagc aaacgcgat 
      atcacttcg gccatcatc gaccaccga ggtggcacg 
      atgattaac gagtattgg ggcgtttcg ctggaaata 
      cccgaaaat gcgatcccc attggggaa caaaaagaa 
      atctatttt gttatttcc gatccgagg ttgtgtgat 
      aacaacgtg ccgccgctg gatttggaa aacggtgaa 
      gcaatgtta tcgccaatt gtcatgtgc ggaccgcaa 
      ggaactgag tttttgaag cctgtgatc ctcaatatt 
      ccgcattat gccaacaca ttgcccagt ctcgggatc 
      agtttgaaa gcgacagac tccgaacaa gatgtgcac 
      acagactgg gacaacatt ttactgcct agcaatcac 
      gccgccaac agtgtggcc gttaaagtc gatcacttt 
      taa
    mRNA sequence
    • >TC006063 RA
      atgtaggta ccattcgca attaaaata agtgattgg 
      gagtgggtg tacctattg ttctggcac attttgcgt 
      tttccccca atcgattac caacaaccc acaaggtca 
      aatcgctta agacgtttt caaatgcga cagattcta 
      gttgtcgcg ttgttggtc cacagcctt caaccggta 
      acagaaaga gaagggacg gtggacttt cttaagtca 
      gttagttta gttgctaga cgcattccg aaccgacgc 
      tgacttgtc cccttatat atttttttc tctaaactg 
      gaataaatg tgataacat ttatcaatt attacaaag 
      tgtaaaata aggatttct ccccatttc gtgcgagtt 
      ttcagtgaa tttttggga ttgacaacg cgtgtcaag 
      tttaaatgt ccatgttgt tggtgttgt gtctgtgtt 
      gtgtggtgc tgaatttgt tgcacagaa atggataac 
      atagccaac aacaatttg gtttcgaat ttacctgag 
      gaacgcggc tgggagtac aaacaaata tgcgtaacc 
      cgagttccg ggctatggg ttcggaatt gccgtctcc 
      ggaggcaga gacaatccg catttcgcc tccggtgac 
      cccagcatc gctgtcagt gatgttcta gcttctggt 
      ccggccgaa agaattttg caggtcaac gacaggatt 
      atttccgtg aatggaatc tcccttcag aatgttgat 
      tacgccacg gcggttcaa gtgctgagg gattccgga 
      gatacagtg accttagtg attaagagg aggacgact 
      cagcatcag catagtcat agtctcagt gcgtcctac 
      actggagca ccacttgct gccttggga gttaatcct 
      gcggcgaca aaggtcacc cttaccaaa aattccaag 
      aaagaagat ttcggcata gtcctgggc tgcaagctc 
      ttcgttaaa gagatctcg tcaaaagcc cgcgaccaa 
      ctcctcaac accaaccaa gtccttgtc gaaggcgac 
      tttatcaca aaaatcaac aacacgaac tgtaacgac 
      ttaatgtcg ctcaaagag gctaaaaaa atcatcgac 
      ggtacaaaa gacaaacta acaatctca atagcacgt 
      gactttaca tcgaatatt tctcatcag acacaaagt 
      tcaataagc accactggc gctgttaac aatttttac 
      aaaggtgat gacttttta acatcgcag agttattca 
      aatcagaat ttgtacgtg caaccaccg accagaaac 
      cccaacctc aatacgagt ggggggttt gaggataag 
      ttggaccca aatagtgga ccgaattct ttggttgat 
      gataagagc aatttggca ccgagggga agaacacgg 
      gggccctta tcagaggct aatttgaac cagttggat 
      aataggaat agtttaggg gggtatggc aggtccaag 
      gggagagat gagccgcct aggcctccg cccccacga 
      gcagaagat tattacagt tctcgccga caaatctac 
      gatgatgat ccaattatc cagaagaag caaccgata 
      cccgacgct cgattcgtg acattccac aaagagggt 
      tctgtggga attcgtcta actggtggc aactttgtc 
      ggaattttc gtaacagcc gtgcaacca ggaagtcca 
      gcatccctt caaggtttg caacctggt gataaaatc 
      ctcaaagtg aatgatatg gatatgact ggcgtaacg 
      cgagaagaa gccgttctt ttcctctta agtctccaa 
      gatcgcatc gaactaatt gttcaatac tgcaaagaa 
      gaatacgac aatatagtc gcgtcccaa aaaggcgac 
      agtttttac atcaagacc cactttcat tacgacaat 
      ccggcaaaa ggtgaaatg tctttccgt tccggagac 
      attttccac gtcatcgat acgctgtat aacggagtc 
      gtgggctcg tggcaagtg tacagaatt ggacgcaac 
      aatcaagaa gtgcaaaag ggaattatt ccgaataaa 
      gctagagcc gaggaactg gcaacggct caattcaac 
      gcaacgaaa aaagaaatg tcggctaat gaatcaaga 
      gggagcttc ttcaggaga cgaaggagt tcgaacaga 
      cggtcgaaa agtctggga aaagagcat tgggatgat 
      attttgttt tccgacact gtttccaaa tttcctgct 
      tatgaacgc gttttgctc aaacatcct gggtttaca 
      agacctgtt attctcttt gggccgatt gctgacgtc 
      gcaagggag aaactactc aaggatttt ccggataaa 
      ttcatatca cctcaactc gattcaaca aatgaggaa 
      aacacaaaa tcgcagaaa acttcaaac atcatacga 
      ttatcagcg attcgtgac gtcatttca actgggaaa 
      cacgccctt ttggacata actcccaac gcggttgaa 
      cgcttgaat tatgcccaa atttaccca attgtagtg 
      tttttcaaa gctgagagc aagattgtg atcaagcag 
      ttgcgtcag ggcttgccc aagtctgcg cacaaaagt 
      tcgaaaaaa ttgctagaa caatgccaa aaactggac 
      aaagtgtgg agccacatt ttcagtgct accataaca 
      atgaacgac aatacggat aactggtat cgcaaagtt 
      cgtgatatt attgataag caacaaagt ggcgccctc 
      tggatgagc gaaagcaag cccgaagaa tcactctca 
      gacgatttc ctgttccca atgtcatct ttgcgacta 
      tcatacgct agtagtcct gaaagtgac ttggaacac 
      agtcctggc cctccagtc tcttcaaat tcaccaaca 
      gctggacgc ttaacaaaa gccagttct gatccaagt 
      gttgtaatg cctgatagt agcaccaca gatgtttta 
      ccgccatat cagccaaac tacgactct aggtatggt 
      tttaacaac aatcaatcg aatgaccaa gttaatttg 
      accaaagaa ccgaactat ttgtccaat caagatatt 
      cctggtagt ttggaccag tatggcaaa agtgggtta 
      tataatact caaatggac acgagaact gattttggg 
      aatgtgcaa cataagaga caagttagt agtcttcat 
      catgggtat aaccatccc aagagtcat agcataaac 
      gagcaaagt ccgcgaagg agtcccaag gaaggggtt 
      tatgggcaa gtccccgat ttaccaccg cgcattgac 
      cgcgcaatc aaacccatc agtttgtta accacaccc 
      agcaaaata cccaacggc cgttcggca catgaaaga 
      cttttcggt accaaaccg ggtcaagct ctggataat 
      ccggaacaa aacaacaac gataataac aatttcagc 
      aacaagttg ggctcatta gaacgaagt cagaactca 
      aagtcggat tctctttca agttacgat tctttcaac 
      aaacagacg aataatcga atcggtccg aacgctcac 
      gacgatctc aaaacaacc ctgtccaaa atcgagcct 
      ggctctgtc cagaatagt ccgtccaaa tacaattct 
      ggcaatagg tgggaccaa aacccacac agacacaac 
      tcgaagctg gatttgaaa tatggtttg cctcaaaat 
      gaccacaat gacaatagt ccgcgcaat tctcgcgag 
      ttggataag gaatcgcgt ccaatggaa aatatgagt 
      ccgagagga tctgtcgaa cgtgatatt tatcgatac 
      tcgaggtct cctgcgaaa aaggggcac gtggaaacc 
      aaaacagat tatggtaaa tacagtcgg aataacagt 
      tccgcacaa gattatgcg aggagttcg catcaggat 
      ttgagtcag tcgcatttg agtgtgcac caagtgcct 
      ccacatcac cactcgccc atgaagagt ccaccggtg 
      caccacaat gggtttgat aatggaggt catatggga 
      cctcctaac ggtaatggg tataagccg gtgccgcct 
      ccgaaaccg aaaaattac aaacctcca tataaaggg 
      caacagggt tatggggaa gggatggtg caaccacct 
      atgccgaca taccaacat gggaagagt cacagtaat 
      ccagtggac caaagggcc caaaacatg aactactat 
      tacaacatg ccacaaaac aacagttgg cacactgaa 
      acggccgtc cgatttaat tccgaaatg cacggtcat 
      tacgacatg gctcctcca tacgaaacg aggagacac 
      ccaagcata tcatacgcc tccaggagt gaaataaca 
      cccattgcc caacacgat aacccatat gggatggat 
      ggaggcgat atgttgatg gatgcacgg cctccggaa 
      cgcagcaat attgtcgat ttgcaaggg tcaagggaa 
      caaagagga tcggcattt gagttgtac agaaaacct 
      gtacatcat aatttaagg gttcaggat gatggcgct 
      ttgttcgga tcacagcct aacataatc caaaaacta 
      aacactaat aactcgcct aaagtaacg ccaaaacta 
      accaaaacg caatcgttc aaacaccaa cgctccgaa 
      ggcggcggt ctcagcgca gcgaaaatc ttccgaaag 
      ttttcgttt aaatcgaga agtagggaa ggcacaccc 
      aagatgccg aaaaaatta tcagtgaaa cccaaagat 
      gaccgaatg cgaccgagc aaacgcgat atcacttcg 
      gccatcatc gaccaccga ggtggcacg atgattaac 
      gagtattgg ggcgtttcg ctggaaata cccgaaaat 
      gcgatcccc attggggaa caaaaagaa atctatttt 
      gttatttcc gatccgagg ttgtgtgat aacaacgtg 
      ccgccgctg gatttggaa aacggtgaa gcaatgtta 
      tcgccaatt gtcatgtgc ggaccgcaa ggaactgag 
      tttttgaag cctgtgatc ctcaatatt ccgcattat 
      gccaacaca ttgcccagt ctcgggatc agtttgaaa 
      gcgacagac tccgaacaa gatgtgcac acagactgg 
      gacaacatt ttactgcct agcaatcac gccgccaac 
      agtgtggcc gttaaagtc gatcacttt taaatttat 
      tatttaatc agcgacaaa tttgctcaa gtttgccat 
      tttttatta ctatttcgc tctcattat atttttatt 
      ttaaatgta tttctttta ttttttaga ctgatgtaa 
      ctgcaactt gtttgtaat tttatttgt aaataaacg 
      aaatttatt tta