Status
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Gene information
General information
TC006062 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2220360..2316218
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006062
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04062

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006062 RA
      MERKRSVRR QRRYERPFA KKCKDLCRQ FIAFLFSNV 
      GIIGLVVSY TIAGAFIFR FVEKKYQMQ VRLDVLRIR 
      NETVSKFWN KTLEMNTFA EADYKKLLL EELLIYQKR 
      MVKFIQRGY DGENYSDKW SFAGAFLYS LTVITTIGY 
      GNISPHTIE GKITTIVYA IIGMPLFLL YLSNIGDIM 
      ARSFKWVYA NCCLCRWCP GVAKRRAER KRERARLEE 
      GEEEEEEEY EVGMYQNYG DFYKQRPSI VSDVSQGVT 
      EGGSEESYD VQTVTVPVT ICLMIMVGY ICGGALLFC 
      KWEDWEFMD AFYFCFISL STIGFGDLV PGDKIYRRG 
      DDEFLVDEV LELTFVICA MYLMVGMAL IAMCFNLMQ 
      EEVIHKIRT TLKLTPGPI QSRRERPPR DGNPVRPQT 
      DFQDPTKRK LLEIHVLLC SYCQ
    • >TC006062 RB
      MERKRSVRR QRRYERPFA KKCKDLCRQ FIAFLFSNV 
      GIIGLVVSY TIAGAFIFR FVEKKYQMQ VRLDVLRIR 
      NETVSKFWN KTLEMNTFA EADYKKLLL EELLIYQKR 
      MVKFIQRGY DGENYSDKW SFAGAFLYS LTVITTIGY 
      GNISPHTIE GKITTIVYA IIGMPLFLL YLSNIGDIM 
      ARSFKWVYA NCCLCRWCP GVAKRRAER KRERARLEE 
      GEEEEEEEY EVGMYQNYG DFYKQRPSI VSDVSQGVT 
      EGGSEESYD VQTVTVPVT ICLMIMVGY ICGGALLFC 
      KWEDWEFMD AFYFCFISL STIGFGDLV PGDKIYRRG 
      DDEFLVDEV LELTFVICA MYLMVGMAL IAMCFNLMQ 
      EEVIHKIRT TLKLTPGPI QSRRERPPR DGNPVRPQT 
      DFQDPTKLV ALLVHSLQP VTEREGTVD FLKSVSLVA 
      RRIPNRR
    CDS sequence
    • >TC006062 RA
      atggaacgc aagaggtct gtcaggaga cagcgaaga 
      tatgagaga cctttcgca aaaaagtgc aaagattta 
      tgcagacaa tttatcgct tttctgttt agtaacgtc 
      ggaatcatt ggacttgtg gtcagctac acgatcgcc 
      ggcgcattc atattccgc ttcgtcgaa aagaagtac 
      cagatgcaa gtgaggctc gacgtgctt cgaatccgc 
      aacgaaacc gtatcgaag ttctggaac aagaccttg 
      gagatgaac acttttgcc gaagcggat tataagaaa 
      cttctatta gaagagctc ctaatctac cagaaaaga 
      atggtcaag ttcatccag cgcgggtac gacggagag 
      aactattcc gacaagtgg tcgttcgcc ggcgctttt 
      ctttattca ctcacggtc atcaccaca attggctat 
      ggcaacatt tcgcctcac acaatcgaa ggtaaaata 
      acaactata gtttacgca ataatagga atgcccctc 
      ttcctccta tacttatcc aacattggc gatatcatg 
      gcccgttcc ttcaagtgg gtttacgcc aactgctgc 
      ctctgtaga tggtgtcca ggtgtggcg aagcgacgc 
      gctgagagg aaacgggaa cgcgcccgt ctggaggag 
      ggggaggag gaggaggag gaggaatat gaggtcgga 
      atgtaccag aattacggc gatttttac aagcaaaga 
      ccatcgatt gtgagtgat gtgagtcaa ggggtgacc 
      gaaggagga agcgaggag tcgtacgat gtgcagacg 
      gtcactgtg ccggtcacc atctgtctg atgattatg 
      gtgggatat atatgcggc ggcgccctt ttgttttgt 
      aaatgggaa gactgggaa ttcatggac gctttctat 
      ttctgcttc atatctttg agcaccatc ggcttcggg 
      gatttggtc cccggtgat aagatttac cgacgcgga 
      gacgacgaa ttcctcgtt gacgaagtc ttggaatta 
      acttttgtc atatgtgcc atgtatctc atggtgggg 
      atggccctc atcgccatg tgctttaat ctgatgcag 
      gaggaagtt atacacaaa atacgaaca acactgaaa 
      ctcactcct ggaccgatc caatcgcga cgagaacgt 
      cctccaagg gatggaaat ccagtgaga ccccagact 
      gacttccag gacccgaca aagcgtaaa ttgttggaa 
      attcatgtt ttgttatgt agttattgc cagtaa
    • >TC006062 RB
      atggaacgc aagaggtct gtcaggaga cagcgaaga 
      tatgagaga cctttcgca aaaaagtgc aaagattta 
      tgcagacaa tttatcgct tttctgttt agtaacgtc 
      ggaatcatt ggacttgtg gtcagctac acgatcgcc 
      ggcgcattc atattccgc ttcgtcgaa aagaagtac 
      cagatgcaa gtgaggctc gacgtgctt cgaatccgc 
      aacgaaacc gtatcgaag ttctggaac aagaccttg 
      gagatgaac acttttgcc gaagcggat tataagaaa 
      cttctatta gaagagctc ctaatctac cagaaaaga 
      atggtcaag ttcatccag cgcgggtac gacggagag 
      aactattcc gacaagtgg tcgttcgcc ggcgctttt 
      ctttattca ctcacggtc atcaccaca attggctat 
      ggcaacatt tcgcctcac acaatcgaa ggtaaaata 
      acaactata gtttacgca ataatagga atgcccctc 
      ttcctccta tacttatcc aacattggc gatatcatg 
      gcccgttcc ttcaagtgg gtttacgcc aactgctgc 
      ctctgtaga tggtgtcca ggtgtggcg aagcgacgc 
      gctgagagg aaacgggaa cgcgcccgt ctggaggag 
      ggggaggag gaggaggag gaggaatat gaggtcgga 
      atgtaccag aattacggc gatttttac aagcaaaga 
      ccatcgatt gtgagtgat gtgagtcaa ggggtgacc 
      gaaggagga agcgaggag tcgtacgat gtgcagacg 
      gtcactgtg ccggtcacc atctgtctg atgattatg 
      gtgggatat atatgcggc ggcgccctt ttgttttgt 
      aaatgggaa gactgggaa ttcatggac gctttctat 
      ttctgcttc atatctttg agcaccatc ggcttcggg 
      gatttggtc cccggtgat aagatttac cgacgcgga 
      gacgacgaa ttcctcgtt gacgaagtc ttggaatta 
      acttttgtc atatgtgcc atgtatctc atggtgggg 
      atggccctc atcgccatg tgctttaat ctgatgcag 
      gaggaagtt atacacaaa atacgaaca acactgaaa 
      ctcactcct ggaccgatc caatcgcga cgagaacgt 
      cctccaagg gatggaaat ccagtgaga ccccagact 
      gacttccag gacccgaca aagcttgtc gcgttgttg 
      gtccacagc cttcaaccg gtaacagaa agagaaggg 
      acggtggac tttcttaag tcagttagt ttagttgct 
      agacgcatt ccgaaccga cgctga
    mRNA sequence
    • >TC006062 RA
      accaaaaaa actataatt aatttttca agaagttct 
      ggtggttgt gaacgttct gagtaattt gtgtgatgg 
      aagcgctcg gatttagcc aacaactgg ggcgttctt 
      aagcaagaa tggcggcaa gcgagcgct tgagatgac 
      aggagcgaa ctatgaccg gaagtgtgt aaaatggaa 
      cgcaagagg tctgtcagg agacagcga agatatgag 
      agacctttc gcaaaaaag tgcaaagat ttatgcaga 
      caatttatc gcttttctg tttagtaac gtcggaatc 
      attggactt gtggtcagc tacacgatc gccggcgca 
      ttcatattc cgcttcgtc gaaaagaag taccagatg 
      caagtgagg ctcgacgtg cttcgaatc cgcaacgaa 
      accgtatcg aagttctgg aacaagacc ttggagatg 
      aacactttt gccgaagcg gattataag aaacttcta 
      ttagaagag ctcctaatc taccagaaa agaatggtc 
      aagttcatc cagcgcggg tacgacgga gagaactat 
      tccgacaag tggtcgttc gccggcgct tttctttat 
      tcactcacg gtcatcacc acaattggc tatggcaac 
      atttcgcct cacacaatc gaaggtaaa ataacaact 
      atagtttac gcaataata ggaatgccc ctcttcctc 
      ctatactta tccaacatt ggcgatatc atggcccgt 
      tccttcaag tgggtttac gccaactgc tgcctctgt 
      agatggtgt ccaggtgtg gcgaagcga cgcgctgag 
      aggaaacgg gaacgcgcc cgtctggag gagggggag 
      gaggaggag gaggaggaa tatgaggtc ggaatgtac 
      cagaattac ggcgatttt tacaagcaa agaccatcg 
      attgtgagt gatgtgagt caaggggtg accgaagga 
      ggaagcgag gagtcgtac gatgtgcag acggtcact 
      gtgccggtc accatctgt ctgatgatt atggtggga 
      tatatatgc ggcggcgcc cttttgttt tgtaaatgg 
      gaagactgg gaattcatg gacgctttc tatttctgc 
      ttcatatct ttgagcacc atcggcttc ggggatttg 
      gtccccggt gataagatt taccgacgc ggagacgac 
      gaattcctc gttgacgaa gtcttggaa ttaactttt 
      gtcatatgt gccatgtat ctcatggtg gggatggcc 
      ctcatcgcc atgtgcttt aatctgatg caggaggaa 
      gttatacac aaaatacga acaacactg aaactcact 
      cctggaccg atccaatcg cgacgagaa cgtcctcca 
      agggatgga aatccagtg agaccccag actgacttc 
      caggacccg acaaagcgt aaattgttg gaaattcat 
      gttttgtta tgtagttat tgccagtaa aactttatc 
      tgttatccg actgaagtt taattaatt attccactt 
      ggcgcaaaa gaagcgagt taatggtaa aaatatctt 
      gtgttaaat tacagtttt aaagtccgg gaccgagtg 
      cttggaaac ttgctcatc aactaaagt ttcactatt 
      tgt
    • >TC006062 RB
      accaaaaaa actataatt aatttttca agaagttct 
      ggtggttgt gaacgttct gagtaattt gtgtgatgg 
      aagcgctcg gatttagcc aacaactgg ggcgttctt 
      aagcaagaa tggcggcaa gcgagcgct tgagatgac 
      aggagcgaa ctatgaccg gaagtgtgt aaaatggaa 
      cgcaagagg tctgtcagg agacagcga agatatgag 
      agacctttc gcaaaaaag tgcaaagat ttatgcaga 
      caatttatc gcttttctg tttagtaac gtcggaatc 
      attggactt gtggtcagc tacacgatc gccggcgca 
      ttcatattc cgcttcgtc gaaaagaag taccagatg 
      caagtgagg ctcgacgtg cttcgaatc cgcaacgaa 
      accgtatcg aagttctgg aacaagacc ttggagatg 
      aacactttt gccgaagcg gattataag aaacttcta 
      ttagaagag ctcctaatc taccagaaa agaatggtc 
      aagttcatc cagcgcggg tacgacgga gagaactat 
      tccgacaag tggtcgttc gccggcgct tttctttat 
      tcactcacg gtcatcacc acaattggc tatggcaac 
      atttcgcct cacacaatc gaaggtaaa ataacaact 
      atagtttac gcaataata ggaatgccc ctcttcctc 
      ctatactta tccaacatt ggcgatatc atggcccgt 
      tccttcaag tgggtttac gccaactgc tgcctctgt 
      agatggtgt ccaggtgtg gcgaagcga cgcgctgag 
      aggaaacgg gaacgcgcc cgtctggag gagggggag 
      gaggaggag gaggaggaa tatgaggtc ggaatgtac 
      cagaattac ggcgatttt tacaagcaa agaccatcg 
      attgtgagt gatgtgagt caaggggtg accgaagga 
      ggaagcgag gagtcgtac gatgtgcag acggtcact 
      gtgccggtc accatctgt ctgatgatt atggtggga 
      tatatatgc ggcggcgcc cttttgttt tgtaaatgg 
      gaagactgg gaattcatg gacgctttc tatttctgc 
      ttcatatct ttgagcacc atcggcttc ggggatttg 
      gtccccggt gataagatt taccgacgc ggagacgac 
      gaattcctc gttgacgaa gtcttggaa ttaactttt 
      gtcatatgt gccatgtat ctcatggtg gggatggcc 
      ctcatcgcc atgtgcttt aatctgatg caggaggaa 
      gttatacac aaaatacga acaacactg aaactcact 
      cctggaccg atccaatcg cgacgagaa cgtcctcca 
      agggatgga aatccagtg agaccccag actgacttc 
      caggacccg acaaagctt gtcgcgttg ttggtccac 
      agccttcaa ccggtaaca gaaagagaa gggacggtg 
      gactttctt aagtcagtt agtttagtt gctagacgc 
      attccgaac cgacgctga cttgtcccc ttatatatt 
      tttttctct aaactggaa taaatgtga taacattta 
      tcaattatt acaaagtgt aaaataagg atttctccc 
      catttcgtg cgagttttc agtgaattt ttgggattg 
      acaacgcgt gtcaagttt aaatgtcca tgttgttgg 
      tgttgtgtc tgtgttgtg tggtgctga atttgttgc 
      acagaaatg gataacata gccaacaac aatttggtt 
      tcgaattta cctgaggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY