Status
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Gene information
General information
TC006061 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2342052..2345775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006061
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04061

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006061 RA
      MATSFRFLY WRVCCYFCT KKPKRKRSK RGSTRSSAR 
      MTPEGRFSR SRNPSFRRS MRTSTKSAD SGFGRSEIG 
      HYSHSDTEL RYHEDPRDQ RPSRGSSLP RMRPSQTSR 
      FSDSSVPQN RRKTSQTRK SGSLDRRKD HEMIEMDPI 
      ILAKTPILC NKYVVDKNE ITSNNIPGI TMRELEGQG 
      AKSQSRNPQ YLELPRASS PGSSSEGSR RSPRPRRKK 
      SPVRHNPSP KMMTPLGYG HRSKYLDDP DSEEEFYED 
      EDDYYEIVR TRIRPVPIW LCVFLVVSY IIAGAFLFK 
      SWENWELLD AAYFCFITL TTIGFGDLV PAKGVTHDT 
      LGDGVIDDV DVNRATISI ALCSLYLLF GIALLAMSF 
      NLVQEEVIA KVKRVAKTL GIIKKDEED SDYSEDDV
    CDS sequence
    • >TC006061 RA
      atggccaca tctttccga tttctctac tggcgggtc 
      tgttgttac ttttgtacc aaaaaaccg aagagaaaa 
      cgctcgaaa cgtggaagt acacgaagt agtgctcgg 
      atgactcct gagggtcgt ttttcacgc tcgaggaat 
      ccctcgttt cgacgatca atgcgaaca tcaacgaaa 
      tcggccgat agtggcttc gggcgctca gaaatcggc 
      cattactcg cattcggat acggaatta aggtaccac 
      gaggacccc cgcgaccag cgcccctcc cgcggctcc 
      agcctgccc cgcatgcgc ccctcccaa acctcccgc 
      ttctccgac agcagcgtc ccccaaaac cggcgcaaa 
      acctcgcaa acgcgcaaa agcggctct ttggaccgc 
      cgcaaagac cacgaaatg atcgaaatg gaccccatc 
      atcttagcc aaaaccccc atcctctgc aacaaatac 
      gtggtggac aagaacgaa atcacctcc aacaacatc 
      ccggggatc acaatgcgc gagttggaa ggccagggg 
      gcgaagtcc caatcccgc aaccctcag taccttgag 
      ctaccacgc gcctcatcc ccgggatca tcctcagag 
      ggttccagg cggagcccc cgacctagg aggaagaag 
      tccccagtg cgccataac ccaagcccc aaaatgatg 
      actcctttg gggtacggg cataggagt aaatatctg 
      gatgatcca gactccgag gaggagttt tatgaggac 
      gaggatgat tattatgag attgtgagg acgaggatt 
      cgtccggtg cccatctgg ttgtgtgtt tttctcgta 
      gttagttat attattgca ggggcgttt ttgttcaag 
      tcttgggag aattgggag ttgttagat gcggcttat 
      ttttgtttt attactttg acaacgatt gggtttggg 
      gatttggtg ccggcgaag ggagtgact catgatact 
      ctaggtgat ggggttatt gatgatgtt gatgtgaat 
      cgagcaacg attagtata gctttatgt tcgttgtat 
      ttattgttt gggattgca cttttggcg atgagtttt 
      aatctcgtt caggaggaa gttatcgcc aaggtgaag 
      cgcgtggcg aagactttg gggattatt aagaaggat 
      gaagaggat tcggattat tcggaagat gatgtttaa
    mRNA sequence
    • >TC006061 RA
      gcccaaaac ccagtgggg caaaatcgt cacaatttt 
      ctacgccat cttgggaat ccccctaat gctcctctg 
      tctctccaa tattggtga catcatggc cacatcttt 
      ccgatttct ctactggcg ggtctgttg ttacttttg 
      taccaaaaa accgaagag aaaacgctc gaaacgtgg 
      aagtacacg aagtagtgc tcggatgac tcctgaggg 
      tcgtttttc acgctcgag gaatccctc gtttcgacg 
      atcaatgcg aacatcaac gaaatcggc cgatagtgg 
      cttcgggcg ctcagaaat cggccatta ctcgcattc 
      ggatacgga attaaggta ccacgagga cccccgcga 
      ccagcgccc ctcccgcgg ctccagcct gccccgcat 
      gcgcccctc ccaaacctc ccgcttctc cgacagcag 
      cgtccccca aaaccggcg caaaacctc gcaaacgcg 
      caaaagcgg ctctttgga ccgccgcaa agaccacga 
      aatgatcga aatggaccc catcatctt agccaaaac 
      ccccatcct ctgcaacaa atacgtggt ggacaagaa 
      cgaaatcac ctccaacaa catcccggg gatcacaat 
      gcgcgagtt ggaaggcca gggggcgaa gtcccaatc 
      ccgcaaccc tcagtacct tgagctacc acgcgcctc 
      atccccggg atcatcctc agagggttc caggcggag 
      cccccgacc taggaggaa gaagtcccc agtgcgcca 
      taacccaag ccccaaaat gatgactcc tttggggta 
      cgggcatag gagtaaata tctggatga tccagactc 
      cgaggagga gttttatga ggacgagga tgattatta 
      tgagattgt gaggacgag gattcgtcc ggtgcccat 
      ctggttgtg tgtttttct cgtagttag ttatattat 
      tgcaggggc gtttttgtt caagtcttg ggagaattg 
      ggagttgtt agatgcggc ttatttttg ttttattac 
      tttgacaac gattgggtt tggggattt ggtgccggc 
      gaagggagt gactcatga tactctagg tgatggggt 
      tattgatga tgttgatgt gaatcgagc aacgattag 
      tatagcttt atgttcgtt gtatttatt gtttgggat 
      tgcactttt ggcgatgag ttttaatct cgttcagga 
      ggaagttat cgccaaggt gaagcgcgt ggcgaagac 
      tttggggat tattaagaa ggatgaaga ggattcgga 
      ttattcgga agatgatgt ttaaagcaa taaattgga 
      gtttcgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY