Status
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Gene information
General information
TC006033 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2632036..2644419
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006033
    iBeetle screen iB_04058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006033 RA
      MFKLISIIV FVHHVRSQN PSPCPEIFS YEPRGQEED 
      RWYGVVSLQ TAEDLDGVW LKITLDRPA ELLGNWFGE 
      AHSSDNQEF TIRNPRYKL EAGPPVSVR FFVKYNAAS 
      TIPSLKVIK LNGKTICTS SRDDIVSTT PQLHISQIR 
      PVTSRPNRP NNRPASSEE GAYLNTKPS SSSGNRGSA 
      QGNHGGSSQ ERPSSHGGS SQERPDSYD NHQTNNYNS 
      QSTTSSYNT NNNNNNYSN NRRPGSQSN SISNNHREH 
      ESSTNSGDF AVHRPSQAR PPSKPSTLS KRNVGCGTV 
      AMKASPLIS YGQNTTQGQ WPWHVALYH IQGAQLLYT 
      CGGTLISEN HVLTAAHCV AKPQTNRPI DTKDLSVYL 
      GKYHLKKFG DGTQDRDVT DIFIHPQYN YSVYFNDIA 
      VLKLKTPAD LNNYVRPCC LWEDGTDIE YVLNKLGTV 
      VGWGFDEKR QISDTLMQA QMPVVSTVN CIYSNREFF 
      SQFTFEKTY CAGFRNGTT VCNGDSGGG MVFPKAGTS 
      GHNTVWQIR GIVSVGVAL QGQGVCDPR HYIVFTDVA 
      KHLDWIKQV MRE
    • >TC006033 RB
      MFKLISIIV FVHHVRSQN PSPCPEIFS YEPRGQEED 
      RWYGVVSLQ TAEDLDGVW LKITLDRPA ELLGNWFGE 
      AHSSDNQEF TIRNPRYKL EAGPPVSVR FFVKYNAAS 
      TIPSLKVIK LNGKTICTS SRDDIVSTT PQLHISQIR 
      PVTSRPNRP NNRPASSEE GAYLNTKPS SSSGNRGSA 
      QGNHGGSSQ ERPSSHGGS SQERPDSYD NHQTNNYNS 
      QSTTSSYNT NNNNNNYSN NRRPGSQSN SISNNHREH 
      ESSTNSGRN SNGLSAGEE TTHSDFFPG DFAVHRPSQ 
      ARPPSKPST LSKRNVGCG TVAMKASPL ISYGQNTTQ 
      GQWPWHVAL YHIQGAQLL YTCGGTLIS ENHVLTAAH 
      CVAKPQTNR PIDTKDLSV YLGKYHLKK FGDGTQDRD 
      VTDIFIHPQ YNYSVYFND IAVLKLKTP ADLNNYVRP 
      CCLWEDGTD IEYVLNKLG TVVGWGFDE KRQISDTLM 
      QAQMPVVST VNCIYSNRE FFSQFTFEK TYCAGFRNG 
      TTVCNGDSG GGMVFPKAG TSGHNTVWQ IRGIVSVGV 
      ALQGQGVCD PRHYIVFTD VAKHLDWIK QVMRE
    CDS sequence
    • >TC006033 RA
      atgtttaaa ctgatctca atcatcgtc ttcgtgcac 
      cacgtccgc agccagaac ccctcaccc tgtcccgag 
      atcttctcc tacgagccc cgcggccag gaggaggac 
      cgatggtac ggcgtcgtt tccctgcaa accgccgaa 
      gacctcgac ggcgtttgg ctcaaaatc accctcgac 
      aggcccgcc gaactccta gggaactgg ttcggcgaa 
      gcgcattcg agcgataac caggagttt acgatacga 
      aaccctaga tataagctg gaggctggg ccccctgtc 
      tctgttcgt ttctttgtc aagtataat gccgcttcg 
      acgattcct agtctcaaa gtgattaaa ttgaacggg 
      aaaacgatt tgtacgagt agtagagac gatatagtt 
      tcgaccaca ccgcagtta catatcagt caaattaga 
      ccggtgact agtagaccg aatagaccg aataataga 
      ccggcgagt agtgaggaa ggcgcctac ttgaacacg 
      aaaccgagc agcagttcg ggaaatagg gggtcggcg 
      caaggaaat catgggggc tcttcacaa gaaaggcca 
      agcagtcat gggggctcc tcacaagaa aggccagac 
      agttacgac aaccaccag accaataat tacaattcg 
      caaagcacg accagcagc tacaacacc aacaacaac 
      aataacaac tatagcaac aacagacga cccggaagc 
      cagtcgaac tcaatcagc aacaatcac cgcgaacac 
      gaatcgtcg actaactca ggtgacttt gccgtgcac 
      cgccccagt caagcgcgc cccccgtcc aagccctca 
      accctaagc aaacgaaac gtaggatgt ggaacagtg 
      gccatgaag gcaagccct ctgatttca tacggccag 
      aacacaacc caaggtcag tggccctgg cacgtcgcc 
      ctgtaccac atccagggg gcccaactc ttatacacc 
      tgcgggggc accctgatc tccgaaaac cacgtcctc 
      acagccgcc cactgcgtg gccaagccc caaaccaac 
      cgacctatc gataccaaa gacctttca gtctactta 
      gggaaatat cacctgaaa aaattcggc gacgggacg 
      caagaccga gacgtgact gacattttc atacatccg 
      cagtacaat tacagcgtc tattttaac gatattgca 
      gtattgaag ctcaaaaca ccggctgat ttgaacaat 
      tatgtaagg ccttgctgc ttgtgggaa gatggcact 
      gacattgaa tacgtcctc aacaagtta gggacggtg 
      gtcggttgg ggcttcgac gagaagcgc cagatctcc 
      gacacgctg atgcaggcg cagatgccg gtggtttcc 
      accgtcaat tgcatttac tccaaccgc gagtttttc 
      tcgcagttt acgttcgaa aaaacgtac tgtgccggt 
      ttccgtaac ggtacaact gtctgcaac ggcgactcc 
      gggggcggt atggtgttc cccaaggcg gggacaagt 
      ggccacaac accgtctgg caaatccgg ggcattgtc 
      tcggttggg gtggccctc caaggacag ggggtttgc 
      gacccgcgg cattatatc gtgtttacg gacgttgcc 
      aaacattta gactggatt aagcaagtt atgagggaa 
      tag
    • >TC006033 RB
      atgtttaaa ctgatctca atcatcgtc ttcgtgcac 
      cacgtccgc agccagaac ccctcaccc tgtcccgag 
      atcttctcc tacgagccc cgcggccag gaggaggac 
      cgatggtac ggcgtcgtt tccctgcaa accgccgaa 
      gacctcgac ggcgtttgg ctcaaaatc accctcgac 
      aggcccgcc gaactccta gggaactgg ttcggcgaa 
      gcgcattcg agcgataac caggagttt acgatacga 
      aaccctaga tataagctg gaggctggg ccccctgtc 
      tctgttcgt ttctttgtc aagtataat gccgcttcg 
      acgattcct agtctcaaa gtgattaaa ttgaacggg 
      aaaacgatt tgtacgagt agtagagac gatatagtt 
      tcgaccaca ccgcagtta catatcagt caaattaga 
      ccggtgact agtagaccg aatagaccg aataataga 
      ccggcgagt agtgaggaa ggcgcctac ttgaacacg 
      aaaccgagc agcagttcg ggaaatagg gggtcggcg 
      caaggaaat catgggggc tcttcacaa gaaaggcca 
      agcagtcat gggggctcc tcacaagaa aggccagac 
      agttacgac aaccaccag accaataat tacaattcg 
      caaagcacg accagcagc tacaacacc aacaacaac 
      aataacaac tatagcaac aacagacga cccggaagc 
      cagtcgaac tcaatcagc aacaatcac cgcgaacac 
      gaatcgtcg actaactca ggtaggaat tccaacggc 
      ttatcagcc ggcgaggaa acgacccac tctgacttc 
      ttcccaggt gactttgcc gtgcaccgc cccagtcaa 
      gcgcgcccc ccgtccaag ccctcaacc ctaagcaaa 
      cgaaacgta ggatgtgga acagtggcc atgaaggca 
      agccctctg atttcatac ggccagaac acaacccaa 
      ggtcagtgg ccctggcac gtcgccctg taccacatc 
      cagggggcc caactctta tacacctgc gggggcacc 
      ctgatctcc gaaaaccac gtcctcaca gccgcccac 
      tgcgtggcc aagccccaa accaaccga cctatcgat 
      accaaagac ctttcagtc tacttaggg aaatatcac 
      ctgaaaaaa ttcggcgac gggacgcaa gaccgagac 
      gtgactgac attttcata catccgcag tacaattac 
      agcgtctat tttaacgat attgcagta ttgaagctc 
      aaaacaccg gctgatttg aacaattat gtaaggcct 
      tgctgcttg tgggaagat ggcactgac attgaatac 
      gtcctcaac aagttaggg acggtggtc ggttggggc 
      ttcgacgag aagcgccag atctccgac acgctgatg 
      caggcgcag atgccggtg gtttccacc gtcaattgc 
      atttactcc aaccgcgag tttttctcg cagtttacg 
      ttcgaaaaa acgtactgt gccggtttc cgtaacggt 
      acaactgtc tgcaacggc gactccggg ggcggtatg 
      gtgttcccc aaggcgggg acaagtggc cacaacacc 
      gtctggcaa atccggggc attgtctcg gttggggtg 
      gccctccaa ggacagggg gtttgcgac ccgcggcat 
      tatatcgtg tttacggac gttgccaaa catttagac 
      tggattaag caagttatg agggaatag
    mRNA sequence
    • >TC006033 RA
      aattcaaaa tttggcgct cgtctctag gtcaagttt 
      aaactcgga aattcctac tacataaca aaataaatg 
      ctcgtgttt gtaaacagg tttgtaaag atcagtcta 
      atcgtctta tccgttcag ttgcgagtc ctttaccgt 
      cgaaaactg cacgttact gccttcaag tgtgtgttt 
      ccagaccca atacatcga caatttcaa agaataaat 
      cgccataat taagcgcga cgatgttta aactgatct 
      caatcatcg tcttcgtgc accacgtcc gcagccaga 
      acccctcac cctgtcccg agatcttct cctacgagc 
      cccgcggcc aggaggagg accgatggt acggcgtcg 
      tttccctgc aaaccgccg aagacctcg acggcgttt 
      ggctcaaaa tcaccctcg acaggcccg ccgaactcc 
      tagggaact ggttcggcg aagcgcatt cgagcgata 
      accaggagt ttacgatac gaaacccta gatataagc 
      tggaggctg ggccccctg tctctgttc gtttctttg 
      tcaagtata atgccgctt cgacgattc ctagtctca 
      aagtgatta aattgaacg ggaaaacga tttgtacga 
      gtagtagag acgatatag tttcgacca caccgcagt 
      tacatatca gtcaaatta gaccggtga ctagtagac 
      cgaatagac cgaataata gaccggcga gtagtgagg 
      aaggcgcct acttgaaca cgaaaccga gcagcagtt 
      cgggaaata gggggtcgg cgcaaggaa atcatgggg 
      gctcttcac aagaaaggc caagcagtc atgggggct 
      cctcacaag aaaggccag acagttacg acaaccacc 
      agaccaata attacaatt cgcaaagca cgaccagca 
      gctacaaca ccaacaaca acaataaca actatagca 
      acaacagac gacccggaa gccagtcga actcaatca 
      gcaacaatc accgcgaac acgaatcgt cgactaact 
      caggtgact ttgccgtgc accgcccca gtcaagcgc 
      gccccccgt ccaagccct caaccctaa gcaaacgaa 
      acgtaggat gtggaacag tggccatga aggcaagcc 
      ctctgattt catacggcc agaacacaa cccaaggtc 
      agtggccct ggcacgtcg ccctgtacc acatccagg 
      gggcccaac tcttataca cctgcgggg gcaccctga 
      tctccgaaa accacgtcc tcacagccg cccactgcg 
      tggccaagc cccaaacca accgaccta tcgatacca 
      aagaccttt cagtctact tagggaaat atcacctga 
      aaaaattcg gcgacggga cgcaagacc gagacgtga 
      ctgacattt tcatacatc cgcagtaca attacagcg 
      tctatttta acgatattg cagtattga agctcaaaa 
      caccggctg atttgaaca attatgtaa ggccttgct 
      gcttgtggg aagatggca ctgacattg aatacgtcc 
      tcaacaagt tagggacgg tggtcggtt ggggcttcg 
      acgagaagc gccagatct ccgacacgc tgatgcagg 
      cgcagatgc cggtggttt ccaccgtca attgcattt 
      actccaacc gcgagtttt tctcgcagt ttacgttcg 
      aaaaaacgt actgtgccg gtttccgta acggtacaa 
      ctgtctgca acggcgact ccgggggcg gtatggtgt 
      tccccaagg cggggacaa gtggccaca acaccgtct 
      ggcaaatcc ggggcattg tctcggttg gggtggccc 
      tccaaggac agggggttt gcgacccgc ggcattata 
      tcgtgttta cggacgttg ccaaacatt tagactgga 
      ttaagcaag ttatgaggg aatagaaat gcgatctgt 
      tttgttgta atactcatt ttgtgatat attgtctaa 
      ataaactac ttttttata c
    • >TC006033 RB
      aattcaaaa tttggcgct cgtctctag gtcaagttt 
      aaactcgga aattcctac tacataaca aaataaatg 
      ctcgtgttt gtaaacagg tttgtaaag atcagtcta 
      atcgtctta tccgttcag ttgcgagtc ctttaccgt 
      cgaaaactg cacgttact gccttcaag tgtgtgttt 
      ccagaccca atacatcga caatttcaa agaataaat 
      cgccataat taagcgcga cgatgttta aactgatct 
      caatcatcg tcttcgtgc accacgtcc gcagccaga 
      acccctcac cctgtcccg agatcttct cctacgagc 
      cccgcggcc aggaggagg accgatggt acggcgtcg 
      tttccctgc aaaccgccg aagacctcg acggcgttt 
      ggctcaaaa tcaccctcg acaggcccg ccgaactcc 
      tagggaact ggttcggcg aagcgcatt cgagcgata 
      accaggagt ttacgatac gaaacccta gatataagc 
      tggaggctg ggccccctg tctctgttc gtttctttg 
      tcaagtata atgccgctt cgacgattc ctagtctca 
      aagtgatta aattgaacg ggaaaacga tttgtacga 
      gtagtagag acgatatag tttcgacca caccgcagt 
      tacatatca gtcaaatta gaccggtga ctagtagac 
      cgaatagac cgaataata gaccggcga gtagtgagg 
      aaggcgcct acttgaaca cgaaaccga gcagcagtt 
      cgggaaata gggggtcgg cgcaaggaa atcatgggg 
      gctcttcac aagaaaggc caagcagtc atgggggct 
      cctcacaag aaaggccag acagttacg acaaccacc 
      agaccaata attacaatt cgcaaagca cgaccagca 
      gctacaaca ccaacaaca acaataaca actatagca 
      acaacagac gacccggaa gccagtcga actcaatca 
      gcaacaatc accgcgaac acgaatcgt cgactaact 
      caggtagga attccaacg gcttatcag ccggcgagg 
      aaacgaccc actctgact tcttcccag gtgactttg 
      ccgtgcacc gccccagtc aagcgcgcc ccccgtcca 
      agccctcaa ccctaagca aacgaaacg taggatgtg 
      gaacagtgg ccatgaagg caagccctc tgatttcat 
      acggccaga acacaaccc aaggtcagt ggccctggc 
      acgtcgccc tgtaccaca tccaggggg cccaactct 
      tatacacct gcgggggca ccctgatct ccgaaaacc 
      acgtcctca cagccgccc actgcgtgg ccaagcccc 
      aaaccaacc gacctatcg ataccaaag acctttcag 
      tctacttag ggaaatatc acctgaaaa aattcggcg 
      acgggacgc aagaccgag acgtgactg acattttca 
      tacatccgc agtacaatt acagcgtct attttaacg 
      atattgcag tattgaagc tcaaaacac cggctgatt 
      tgaacaatt atgtaaggc cttgctgct tgtgggaag 
      atggcactg acattgaat acgtcctca acaagttag 
      ggacggtgg tcggttggg gcttcgacg agaagcgcc 
      agatctccg acacgctga tgcaggcgc agatgccgg 
      tggtttcca ccgtcaatt gcatttact ccaaccgcg 
      agtttttct cgcagttta cgttcgaaa aaacgtact 
      gtgccggtt tccgtaacg gtacaactg tctgcaacg 
      gcgactccg ggggcggta tggtgttcc ccaaggcgg 
      ggacaagtg gccacaaca ccgtctggc aaatccggg 
      gcattgtct cggttgggg tggccctcc aaggacagg 
      gggtttgcg acccgcggc attatatcg tgtttacgg 
      acgttgcca aacatttag actggatta agcaagtta 
      tgagggaat agaaatgcg atctgtttt gttgtaata 
      ctcattttg tgatatatt gtctaaata aactacttt 
      tttatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY