General information | |||
TC033957 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 2651389..2682756 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC033957 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
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Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04057 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva dorsal bent - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva cuticle dorsalcuticularisation not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva cuticle ventralcuticularisation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
mesothorax segment number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen partially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: bad quality of preparation,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC033957 RA
MVMMRLLAL VLTLIHYKT ADAEQVVLL DTTGEPTLD WTRYPYGPQ ANTPGWVEE SFTNFQKGI NWRSYVVCD VAYNNVNNW LWTPFIDRG VANRIYIEI KFTIRDCSL FPGNALSCK ETFSLLYYE FDAATREPP PWDADRYKL IGRIAAGEG RFNSNSEVN INTEVKSIP VTKKGVYFA FRDQGACIS LLAIKVYYI TCPEVTINF AKFPATPTG KEITLIEQA TGSCVPNAE IVGGTPTYL CKGDGKWTL PTGGCKCKA GFQPDVEKQ TCNVCGPGM YKAEVGDEN CLPCPGHST GNDYGLSEC RCNNGYYRA PTDPKNMSC TQPPSAPQN LTVNFVDQS TVALSWAPP DNLGGRTDT VYRIKCDAC SLGLVQYNP HTDTFNDTR VTISGLNAV TTYRFQIFA ENGVSFMRT EAPEYADVT VTTEALVAS SITNVRVSS VMSSEITLA WDAPSAAGD GNDIENDLV ETYEVRWFP RSDMDYSNA SSLLTTDLF ATITGLQQR TEYGLQVRA KTQRGWGAY SPVIFKTTG QVLNTAYVG DDEGIRLQL VAGSIVVVV VVLVVIIVF TVMFLRSRS NDECNKKQP SDCDTLEYR NGEVHHSLD NPPIVTTHT NVTTPLFTG ISGSSRTYI DPHTYEDPN QAVREFARE IDASCITIE AIIGGGEFG DVCKGKLKT NGIDIDVAI KTLKAGSLD KARNDFLTE ASIMGQFEH PNVIFLQGV VTKSNPVMI ITEYMENGS LDTFLRAND GKFQVIQLV GMLRGIASG MQYLSEMNY VHRDLAARN VLVNSQLVC KIADFGLSR EIESATEGA YTTRGGKIP VRWTAPEAI AFRKFTSAS DVWSMGIVC WEVMSYGER PYWNWSNQD VIKSIEKGY RLPAPMDCP EAIYQLMLD CWQKERTHR PTFQSIVKT LDKLIRVPD TLRKIAQNR HLPPCLNPY FRQDLRHVS ELPARESFF DYGLPDVIL LQNYEMSQW LSQERFNLS SDFGSSDQT LFTYQGDTH TPSHTYTKR GAWVTFSNM
- >TC033957 RA
atggtcatg atgcgtctg ctagcgctt gttctaaca ctcatccac tacaaaaca gctgatgcc gagcaagtc gtgcttttg gatacgacc ggcgaaccc accctagac tggaccagg tatccctac ggaccccaa gccaacacg ccggggtgg gtggaagag tcgttcacg aatttccaa aaaggcatc aactggaga tcgtacgtt gtgtgcgac gttgcctac aacaacgtc aataattgg ctatggact ccgttcata gatagagga gtcgccaat cgaatatac attgaaatt aagttcaca atccgggat tgtagccta tttccaggc aatgcctta tcgtgcaag gaaacattt agtttattg tattacgaa tttgacgcc gcgacgcgt gagccgcct ccatgggac gccgacaga tacaagctc atcggtcgc attgcggcc ggcgaaggc cgtttcaat tccaacagc gaagtgaac atcaacacc gaagtaaaa agcatccca gtgacgaaa aagggcgtt tatttcgcg tttcgcgac caaggcgcg tgcatttcc ctcctcgcc atcaaagtg tattacatc acgtgtcca gaagtgacg attaatttc gccaaattt ccggcaaca ccaaccggt aaagaaatc actctcatc gagcaagcg acaggtagt tgcgtgccg aatgcagaa atcgtcggt gggacgccc acttatcta tgcaaaggg gatggaaag tggacgtta ccgaccggg ggatgcaaa tgcaaggct gggtttcaa ccggatgtg gaaaagcag acttgcaat gtgtgtggt cctgggatg tacaaagcg gaggttggt gatgagaat tgtttgccg tgtccggga cattccaca gggaatgat tatggactg tctgagtgc cggtgcaat aatgggtat tatcgggca cctaccgac cccaagaat atgtcctgc actcagcct ccatccgcg ccccaaaac ctcaccgtc aacttcgtg gaccaatca accgtcgcc ttaagttgg gccccacct gacaacctc ggcggtcgc accgacacc gtctaccgc atcaaatgc gacgcttgc agtcttggc ctcgtccaa tacaacccc catacggac acattcaac gacacacgt gtcacaatc tcaggcctg aacgccgtc accacgtac cgcttccaa atcttcgcc gaaaacggc gtctccttc atgcggacg gaagcccca gagtacgcc gacgttacc gtcacgacc gaagcccta gtcgccagc agcatcacc aacgtgcgc gtgtcgtcc gtgatgagc tcggagatc acactcgcc tgggacgcg ccgtcggcg gctggcgac ggcaacgac attgaaaac gacttggtg gaaacgtac gaagtgcgc tggttcccc aggtccgac atggactac agtaacgcg tccagcttg cttacgact gatttattt gcgacaatc actggcttg cagcaaaga actgaatac gggctgcaa gtcagggcc aagacgcag cggggctgg ggggcgtat tcgccggtt atttttaaa actaccgga caggtgctc aatacagcc tatgttggg gatgatgaa ggcattagg ttacaatta gtcgcaggt tcgatcgtc gtagtggtc gttgtcttg gtggtgatc attgttttc acggttatg ttcctcaga tcacgatcg aatgacgaa tgtaataaa aaacaaccc agtgattgt gatacttta gaatatcgc aacggtgaa gttcatcac agcttggat aacccccct atagttaca acccataca aatgtgacg acgccgctg ttcactgga atcagcggc tcctcccgc acctacatc gacccccac acgtacgag gaccccaac caggcggtg cgcgaattc gcgcgcgaa atcgacgcc agctgcatt acaatcgaa gcgattatc ggcggcggc gaattcggc gatgtttgc aaaggcaag ctcaaaacg aacggaatc gacatcgat gtcgcgatc aagacactc aaagccggc tcgttggat aaagcacgc aacgatttc ctcaccgaa gcctcgatc atgggccag ttcgagcac cccaacgtg atattcctc caaggggtg gtcaccaag tccaacccc gttatgatc atcaccgag tatatggag aacgggtcg ctggacacc ttcctccgc gccaacgac ggcaaattc caggtgatc cagctggtg ggcatgctc cggggcata gcctccggt atgcaatac ttgagcgaa atgaactac gtccatcgt gatctagcg gcgagaaat gtcctcgtt aactcgcaa ctcgtttgc aaaatcgcc gacttcggc ttgagtcgc gagatcgag agtgcgaca gaaggggcg tacaccaca cgcgggggc aaaatcccg gtgaggtgg acggcaccc gaagctatc gccttccgg aagttcaca tcggcgagc gatgtctgg tcgatggga atcgtctgc tgggaggtg atgtcctac ggggagagg ccctactgg aattggtcg aatcaggac gtcatcaag agcatagag aaggggtac cgacttccg gcccccatg gactgtcct gaagcgatc taccagctc atgttggac tgttggcag aaggagcgc acccacagg cccacattc caatcgatt gtaaagact ttagataaa ttaatacgt gttccggac acgttgcgg aaaatcgct cagaatcga cacctgcca ccgtgctta aacccatac tttcggcaa gatttgcgt cacgtgtca gaactgccc gcgcgcgag tcgtttttc gattacggc cttccggac gtaatactt ttgcaaaac tacgaaatg tctcagtgg ctttcgcag gaacgtttc aatttgagt tccgatttt ggcagttct gatcagacg ctgttcaca taccaggga gacactcac acgccatca cacacgtac actaaaagg ggcgcttgg gtcactttt agcaacatg taa
- >TC033957 RA
taaaaaaat acgacggaa gccgaaaaa caaaacaaa aatcgtcga aagtgtgtt acacttaag tttttaaac ttttaaaca catttttga ataagacca ccaaaaaat tcaccacta actcaaaaa cttatacct aatattatt tcttgtttg ggcattttt gtgcgtttt acagccagc aatgctatt attatttag tttggcaac actgatcag agggttcgc cactgctgc ctgattaaa agatattta attaattta aaatgaatc tctatatta atagtggca tattgttga ttaactaat tatgttgat tacttatta aaatttaaa gatattgat gattaacat ttattttag ataaaaaat ggaaatatt gactaacac gacaatttt taaatgatt tacgtttac taatgctct gtttgatat aaatacgtt gtagaagtg ccaaataca ttgaatgaa aaaacctca cccagattt gtttacttt tcccttttt gcgataaaa ctgtttaca aacattgcg acaagtgcc aaaaaaatg tcatcggcg cgcgatagt caaatgttt gattgtagt gtgcgcatt ctcatcgtc tcaatcgat acacaatac gaatttttc cggtgtgaa gctggcttt ctaaagatt atcgttggg ttcagtgtg ctcagcgat gctgctgct gaatcgtag cgggccgtg tcggaatgt cgtgaaaat ctcgtcgga aattaacaa aaacgcatc gtttttcgc ccgaaaaca agtgcaatc gccgatggt gtgctgatt atccgaaac ggtgattgt ttacgttgc atcagagtg acctgtgtg ttcattaca gcactgcct tgccgtgta tgtgtgtat atggcagat cttgactaa ataccgtgt tttttcttt cgccaacga acagttatt tgaggaatt ttgcccagt tgagtcatt ttgacaaaa tgtgaccaa catatcatg tcagagaat ggagtagca gcaaagctc gcggtccgc ctcttaagg caggacaaa atcgcatcc tgctattga aaataacag aaaaaaaac atggtcatg atgcgtctg ctagcgctt gttctaaca ctcatccac tacaaaaca gctgatgcc gagcaagtc gtgcttttg gatacgacc ggcgaaccc accctagac tggaccagg tatccctac ggaccccaa gccaacacg ccggggtgg gtggaagag tcgttcacg aatttccaa aaaggcatc aactggaga tcgtacgtt gtgtgcgac gttgcctac aacaacgtc aataattgg ctatggact ccgttcata gatagagga gtcgccaat cgaatatac attgaaatt aagttcaca atccgggat tgtagccta tttccaggc aatgcctta tcgtgcaag gaaacattt agtttattg tattacgaa tttgacgcc gcgacgcgt gagccgcct ccatgggac gccgacaga tacaagctc atcggtcgc attgcggcc ggcgaaggc cgtttcaat tccaacagc gaagtgaac atcaacacc gaagtaaaa agcatccca gtgacgaaa aagggcgtt tatttcgcg tttcgcgac caaggcgcg tgcatttcc ctcctcgcc atcaaagtg tattacatc acgtgtcca gaagtgacg attaatttc gccaaattt ccggcaaca ccaaccggt aaagaaatc actctcatc gagcaagcg acaggtagt tgcgtgccg aatgcagaa atcgtcggt gggacgccc acttatcta tgcaaaggg gatggaaag tggacgtta ccgaccggg ggatgcaaa tgcaaggct gggtttcaa ccggatgtg gaaaagcag acttgcaat gtgtgtggt cctgggatg tacaaagcg gaggttggt gatgagaat tgtttgccg tgtccggga cattccaca gggaatgat tatggactg tctgagtgc cggtgcaat aatgggtat tatcgggca cctaccgac cccaagaat atgtcctgc actcagcct ccatccgcg ccccaaaac ctcaccgtc aacttcgtg gaccaatca accgtcgcc ttaagttgg gccccacct gacaacctc ggcggtcgc accgacacc gtctaccgc atcaaatgc gacgcttgc agtcttggc ctcgtccaa tacaacccc catacggac acattcaac gacacacgt gtcacaatc tcaggcctg aacgccgtc accacgtac cgcttccaa atcttcgcc gaaaacggc gtctccttc atgcggacg gaagcccca gagtacgcc gacgttacc gtcacgacc gaagcccta gtcgccagc agcatcacc aacgtgcgc gtgtcgtcc gtgatgagc tcggagatc acactcgcc tgggacgcg ccgtcggcg gctggcgac ggcaacgac attgaaaac gacttggtg gaaacgtac gaagtgcgc tggttcccc aggtccgac atggactac agtaacgcg tccagcttg cttacgact gatttattt gcgacaatc actggcttg cagcaaaga actgaatac gggctgcaa gtcagggcc aagacgcag cggggctgg ggggcgtat tcgccggtt atttttaaa actaccgga caggtgctc aatacagcc tatgttggg gatgatgaa ggcattagg ttacaatta gtcgcaggt tcgatcgtc gtagtggtc gttgtcttg gtggtgatc attgttttc acggttatg ttcctcaga tcacgatcg aatgacgaa tgtaataaa aaacaaccc agtgattgt gatacttta gaatatcgc aacggtgaa gttcatcac agcttggat aacccccct atagttaca acccataca aatgtgacg acgccgctg ttcactgga atcagcggc tcctcccgc acctacatc gacccccac acgtacgag gaccccaac caggcggtg cgcgaattc gcgcgcgaa atcgacgcc agctgcatt acaatcgaa gcgattatc ggcggcggc gaattcggc gatgtttgc aaaggcaag ctcaaaacg aacggaatc gacatcgat gtcgcgatc aagacactc aaagccggc tcgttggat aaagcacgc aacgatttc ctcaccgaa gcctcgatc atgggccag ttcgagcac cccaacgtg atattcctc caaggggtg gtcaccaag tccaacccc gttatgatc atcaccgag tatatggag aacgggtcg ctggacacc ttcctccgc gccaacgac ggcaaattc caggtgatc cagctggtg ggcatgctc cggggcata gcctccggt atgcaatac ttgagcgaa atgaactac gtccatcgt gatctagcg gcgagaaat gtcctcgtt aactcgcaa ctcgtttgc aaaatcgcc gacttcggc ttgagtcgc gagatcgag agtgcgaca gaaggggcg tacaccaca cgcgggggc aaaatcccg gtgaggtgg acggcaccc gaagctatc gccttccgg aagttcaca tcggcgagc gatgtctgg tcgatggga atcgtctgc tgggaggtg atgtcctac ggggagagg ccctactgg aattggtcg aatcaggac gtcatcaag agcatagag aaggggtac cgacttccg gcccccatg gactgtcct gaagcgatc taccagctc atgttggac tgttggcag aaggagcgc acccacagg cccacattc caatcgatt gtaaagact ttagataaa ttaatacgt gttccggac acgttgcgg aaaatcgct cagaatcga cacctgcca ccgtgctta aacccatac tttcggcaa gatttgcgt cacgtgtca gaactgccc gcgcgcgag tcgtttttc gattacggc cttccggac gtaatactt ttgcaaaac tacgaaatg tctcagtgg ctttcgcag gaacgtttc aatttgagt tccgatttt ggcagttct gatcagacg ctgttcaca taccaggga gacactcac acgccatca cacacgtac actaaaagg ggcgcttgg gtcactttt agcaacatg taaatatgc ttttgtttg atttttgca tgacttttg tgtttctgc ttcctaatt tttcattaa ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
