Status
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Gene information
General information
TC033957 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2651389..2682756
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033957
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04057

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva cuticle dorsalcuticularisation not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle ventralcuticularisation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    mesothorax segment number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen partially transformed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    antenna size shortened - Number of animals showing the phenotype: 8

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033957 RA
      MVMMRLLAL VLTLIHYKT ADAEQVVLL DTTGEPTLD 
      WTRYPYGPQ ANTPGWVEE SFTNFQKGI NWRSYVVCD 
      VAYNNVNNW LWTPFIDRG VANRIYIEI KFTIRDCSL 
      FPGNALSCK ETFSLLYYE FDAATREPP PWDADRYKL 
      IGRIAAGEG RFNSNSEVN INTEVKSIP VTKKGVYFA 
      FRDQGACIS LLAIKVYYI TCPEVTINF AKFPATPTG 
      KEITLIEQA TGSCVPNAE IVGGTPTYL CKGDGKWTL 
      PTGGCKCKA GFQPDVEKQ TCNVCGPGM YKAEVGDEN 
      CLPCPGHST GNDYGLSEC RCNNGYYRA PTDPKNMSC 
      TQPPSAPQN LTVNFVDQS TVALSWAPP DNLGGRTDT 
      VYRIKCDAC SLGLVQYNP HTDTFNDTR VTISGLNAV 
      TTYRFQIFA ENGVSFMRT EAPEYADVT VTTEALVAS 
      SITNVRVSS VMSSEITLA WDAPSAAGD GNDIENDLV 
      ETYEVRWFP RSDMDYSNA SSLLTTDLF ATITGLQQR 
      TEYGLQVRA KTQRGWGAY SPVIFKTTG QVLNTAYVG 
      DDEGIRLQL VAGSIVVVV VVLVVIIVF TVMFLRSRS 
      NDECNKKQP SDCDTLEYR NGEVHHSLD NPPIVTTHT 
      NVTTPLFTG ISGSSRTYI DPHTYEDPN QAVREFARE 
      IDASCITIE AIIGGGEFG DVCKGKLKT NGIDIDVAI 
      KTLKAGSLD KARNDFLTE ASIMGQFEH PNVIFLQGV 
      VTKSNPVMI ITEYMENGS LDTFLRAND GKFQVIQLV 
      GMLRGIASG MQYLSEMNY VHRDLAARN VLVNSQLVC 
      KIADFGLSR EIESATEGA YTTRGGKIP VRWTAPEAI 
      AFRKFTSAS DVWSMGIVC WEVMSYGER PYWNWSNQD 
      VIKSIEKGY RLPAPMDCP EAIYQLMLD CWQKERTHR 
      PTFQSIVKT LDKLIRVPD TLRKIAQNR HLPPCLNPY 
      FRQDLRHVS ELPARESFF DYGLPDVIL LQNYEMSQW 
      LSQERFNLS SDFGSSDQT LFTYQGDTH TPSHTYTKR 
      GAWVTFSNM
    CDS sequence
    • >TC033957 RA
      atggtcatg atgcgtctg ctagcgctt gttctaaca 
      ctcatccac tacaaaaca gctgatgcc gagcaagtc 
      gtgcttttg gatacgacc ggcgaaccc accctagac 
      tggaccagg tatccctac ggaccccaa gccaacacg 
      ccggggtgg gtggaagag tcgttcacg aatttccaa 
      aaaggcatc aactggaga tcgtacgtt gtgtgcgac 
      gttgcctac aacaacgtc aataattgg ctatggact 
      ccgttcata gatagagga gtcgccaat cgaatatac 
      attgaaatt aagttcaca atccgggat tgtagccta 
      tttccaggc aatgcctta tcgtgcaag gaaacattt 
      agtttattg tattacgaa tttgacgcc gcgacgcgt 
      gagccgcct ccatgggac gccgacaga tacaagctc 
      atcggtcgc attgcggcc ggcgaaggc cgtttcaat 
      tccaacagc gaagtgaac atcaacacc gaagtaaaa 
      agcatccca gtgacgaaa aagggcgtt tatttcgcg 
      tttcgcgac caaggcgcg tgcatttcc ctcctcgcc 
      atcaaagtg tattacatc acgtgtcca gaagtgacg 
      attaatttc gccaaattt ccggcaaca ccaaccggt 
      aaagaaatc actctcatc gagcaagcg acaggtagt 
      tgcgtgccg aatgcagaa atcgtcggt gggacgccc 
      acttatcta tgcaaaggg gatggaaag tggacgtta 
      ccgaccggg ggatgcaaa tgcaaggct gggtttcaa 
      ccggatgtg gaaaagcag acttgcaat gtgtgtggt 
      cctgggatg tacaaagcg gaggttggt gatgagaat 
      tgtttgccg tgtccggga cattccaca gggaatgat 
      tatggactg tctgagtgc cggtgcaat aatgggtat 
      tatcgggca cctaccgac cccaagaat atgtcctgc 
      actcagcct ccatccgcg ccccaaaac ctcaccgtc 
      aacttcgtg gaccaatca accgtcgcc ttaagttgg 
      gccccacct gacaacctc ggcggtcgc accgacacc 
      gtctaccgc atcaaatgc gacgcttgc agtcttggc 
      ctcgtccaa tacaacccc catacggac acattcaac 
      gacacacgt gtcacaatc tcaggcctg aacgccgtc 
      accacgtac cgcttccaa atcttcgcc gaaaacggc 
      gtctccttc atgcggacg gaagcccca gagtacgcc 
      gacgttacc gtcacgacc gaagcccta gtcgccagc 
      agcatcacc aacgtgcgc gtgtcgtcc gtgatgagc 
      tcggagatc acactcgcc tgggacgcg ccgtcggcg 
      gctggcgac ggcaacgac attgaaaac gacttggtg 
      gaaacgtac gaagtgcgc tggttcccc aggtccgac 
      atggactac agtaacgcg tccagcttg cttacgact 
      gatttattt gcgacaatc actggcttg cagcaaaga 
      actgaatac gggctgcaa gtcagggcc aagacgcag 
      cggggctgg ggggcgtat tcgccggtt atttttaaa 
      actaccgga caggtgctc aatacagcc tatgttggg 
      gatgatgaa ggcattagg ttacaatta gtcgcaggt 
      tcgatcgtc gtagtggtc gttgtcttg gtggtgatc 
      attgttttc acggttatg ttcctcaga tcacgatcg 
      aatgacgaa tgtaataaa aaacaaccc agtgattgt 
      gatacttta gaatatcgc aacggtgaa gttcatcac 
      agcttggat aacccccct atagttaca acccataca 
      aatgtgacg acgccgctg ttcactgga atcagcggc 
      tcctcccgc acctacatc gacccccac acgtacgag 
      gaccccaac caggcggtg cgcgaattc gcgcgcgaa 
      atcgacgcc agctgcatt acaatcgaa gcgattatc 
      ggcggcggc gaattcggc gatgtttgc aaaggcaag 
      ctcaaaacg aacggaatc gacatcgat gtcgcgatc 
      aagacactc aaagccggc tcgttggat aaagcacgc 
      aacgatttc ctcaccgaa gcctcgatc atgggccag 
      ttcgagcac cccaacgtg atattcctc caaggggtg 
      gtcaccaag tccaacccc gttatgatc atcaccgag 
      tatatggag aacgggtcg ctggacacc ttcctccgc 
      gccaacgac ggcaaattc caggtgatc cagctggtg 
      ggcatgctc cggggcata gcctccggt atgcaatac 
      ttgagcgaa atgaactac gtccatcgt gatctagcg 
      gcgagaaat gtcctcgtt aactcgcaa ctcgtttgc 
      aaaatcgcc gacttcggc ttgagtcgc gagatcgag 
      agtgcgaca gaaggggcg tacaccaca cgcgggggc 
      aaaatcccg gtgaggtgg acggcaccc gaagctatc 
      gccttccgg aagttcaca tcggcgagc gatgtctgg 
      tcgatggga atcgtctgc tgggaggtg atgtcctac 
      ggggagagg ccctactgg aattggtcg aatcaggac 
      gtcatcaag agcatagag aaggggtac cgacttccg 
      gcccccatg gactgtcct gaagcgatc taccagctc 
      atgttggac tgttggcag aaggagcgc acccacagg 
      cccacattc caatcgatt gtaaagact ttagataaa 
      ttaatacgt gttccggac acgttgcgg aaaatcgct 
      cagaatcga cacctgcca ccgtgctta aacccatac 
      tttcggcaa gatttgcgt cacgtgtca gaactgccc 
      gcgcgcgag tcgtttttc gattacggc cttccggac 
      gtaatactt ttgcaaaac tacgaaatg tctcagtgg 
      ctttcgcag gaacgtttc aatttgagt tccgatttt 
      ggcagttct gatcagacg ctgttcaca taccaggga 
      gacactcac acgccatca cacacgtac actaaaagg 
      ggcgcttgg gtcactttt agcaacatg taa
    mRNA sequence
    • >TC033957 RA
      taaaaaaat acgacggaa gccgaaaaa caaaacaaa 
      aatcgtcga aagtgtgtt acacttaag tttttaaac 
      ttttaaaca catttttga ataagacca ccaaaaaat 
      tcaccacta actcaaaaa cttatacct aatattatt 
      tcttgtttg ggcattttt gtgcgtttt acagccagc 
      aatgctatt attatttag tttggcaac actgatcag 
      agggttcgc cactgctgc ctgattaaa agatattta 
      attaattta aaatgaatc tctatatta atagtggca 
      tattgttga ttaactaat tatgttgat tacttatta 
      aaatttaaa gatattgat gattaacat ttattttag 
      ataaaaaat ggaaatatt gactaacac gacaatttt 
      taaatgatt tacgtttac taatgctct gtttgatat 
      aaatacgtt gtagaagtg ccaaataca ttgaatgaa 
      aaaacctca cccagattt gtttacttt tcccttttt 
      gcgataaaa ctgtttaca aacattgcg acaagtgcc 
      aaaaaaatg tcatcggcg cgcgatagt caaatgttt 
      gattgtagt gtgcgcatt ctcatcgtc tcaatcgat 
      acacaatac gaatttttc cggtgtgaa gctggcttt 
      ctaaagatt atcgttggg ttcagtgtg ctcagcgat 
      gctgctgct gaatcgtag cgggccgtg tcggaatgt 
      cgtgaaaat ctcgtcgga aattaacaa aaacgcatc 
      gtttttcgc ccgaaaaca agtgcaatc gccgatggt 
      gtgctgatt atccgaaac ggtgattgt ttacgttgc 
      atcagagtg acctgtgtg ttcattaca gcactgcct 
      tgccgtgta tgtgtgtat atggcagat cttgactaa 
      ataccgtgt tttttcttt cgccaacga acagttatt 
      tgaggaatt ttgcccagt tgagtcatt ttgacaaaa 
      tgtgaccaa catatcatg tcagagaat ggagtagca 
      gcaaagctc gcggtccgc ctcttaagg caggacaaa 
      atcgcatcc tgctattga aaataacag aaaaaaaac 
      atggtcatg atgcgtctg ctagcgctt gttctaaca 
      ctcatccac tacaaaaca gctgatgcc gagcaagtc 
      gtgcttttg gatacgacc ggcgaaccc accctagac 
      tggaccagg tatccctac ggaccccaa gccaacacg 
      ccggggtgg gtggaagag tcgttcacg aatttccaa 
      aaaggcatc aactggaga tcgtacgtt gtgtgcgac 
      gttgcctac aacaacgtc aataattgg ctatggact 
      ccgttcata gatagagga gtcgccaat cgaatatac 
      attgaaatt aagttcaca atccgggat tgtagccta 
      tttccaggc aatgcctta tcgtgcaag gaaacattt 
      agtttattg tattacgaa tttgacgcc gcgacgcgt 
      gagccgcct ccatgggac gccgacaga tacaagctc 
      atcggtcgc attgcggcc ggcgaaggc cgtttcaat 
      tccaacagc gaagtgaac atcaacacc gaagtaaaa 
      agcatccca gtgacgaaa aagggcgtt tatttcgcg 
      tttcgcgac caaggcgcg tgcatttcc ctcctcgcc 
      atcaaagtg tattacatc acgtgtcca gaagtgacg 
      attaatttc gccaaattt ccggcaaca ccaaccggt 
      aaagaaatc actctcatc gagcaagcg acaggtagt 
      tgcgtgccg aatgcagaa atcgtcggt gggacgccc 
      acttatcta tgcaaaggg gatggaaag tggacgtta 
      ccgaccggg ggatgcaaa tgcaaggct gggtttcaa 
      ccggatgtg gaaaagcag acttgcaat gtgtgtggt 
      cctgggatg tacaaagcg gaggttggt gatgagaat 
      tgtttgccg tgtccggga cattccaca gggaatgat 
      tatggactg tctgagtgc cggtgcaat aatgggtat 
      tatcgggca cctaccgac cccaagaat atgtcctgc 
      actcagcct ccatccgcg ccccaaaac ctcaccgtc 
      aacttcgtg gaccaatca accgtcgcc ttaagttgg 
      gccccacct gacaacctc ggcggtcgc accgacacc 
      gtctaccgc atcaaatgc gacgcttgc agtcttggc 
      ctcgtccaa tacaacccc catacggac acattcaac 
      gacacacgt gtcacaatc tcaggcctg aacgccgtc 
      accacgtac cgcttccaa atcttcgcc gaaaacggc 
      gtctccttc atgcggacg gaagcccca gagtacgcc 
      gacgttacc gtcacgacc gaagcccta gtcgccagc 
      agcatcacc aacgtgcgc gtgtcgtcc gtgatgagc 
      tcggagatc acactcgcc tgggacgcg ccgtcggcg 
      gctggcgac ggcaacgac attgaaaac gacttggtg 
      gaaacgtac gaagtgcgc tggttcccc aggtccgac 
      atggactac agtaacgcg tccagcttg cttacgact 
      gatttattt gcgacaatc actggcttg cagcaaaga 
      actgaatac gggctgcaa gtcagggcc aagacgcag 
      cggggctgg ggggcgtat tcgccggtt atttttaaa 
      actaccgga caggtgctc aatacagcc tatgttggg 
      gatgatgaa ggcattagg ttacaatta gtcgcaggt 
      tcgatcgtc gtagtggtc gttgtcttg gtggtgatc 
      attgttttc acggttatg ttcctcaga tcacgatcg 
      aatgacgaa tgtaataaa aaacaaccc agtgattgt 
      gatacttta gaatatcgc aacggtgaa gttcatcac 
      agcttggat aacccccct atagttaca acccataca 
      aatgtgacg acgccgctg ttcactgga atcagcggc 
      tcctcccgc acctacatc gacccccac acgtacgag 
      gaccccaac caggcggtg cgcgaattc gcgcgcgaa 
      atcgacgcc agctgcatt acaatcgaa gcgattatc 
      ggcggcggc gaattcggc gatgtttgc aaaggcaag 
      ctcaaaacg aacggaatc gacatcgat gtcgcgatc 
      aagacactc aaagccggc tcgttggat aaagcacgc 
      aacgatttc ctcaccgaa gcctcgatc atgggccag 
      ttcgagcac cccaacgtg atattcctc caaggggtg 
      gtcaccaag tccaacccc gttatgatc atcaccgag 
      tatatggag aacgggtcg ctggacacc ttcctccgc 
      gccaacgac ggcaaattc caggtgatc cagctggtg 
      ggcatgctc cggggcata gcctccggt atgcaatac 
      ttgagcgaa atgaactac gtccatcgt gatctagcg 
      gcgagaaat gtcctcgtt aactcgcaa ctcgtttgc 
      aaaatcgcc gacttcggc ttgagtcgc gagatcgag 
      agtgcgaca gaaggggcg tacaccaca cgcgggggc 
      aaaatcccg gtgaggtgg acggcaccc gaagctatc 
      gccttccgg aagttcaca tcggcgagc gatgtctgg 
      tcgatggga atcgtctgc tgggaggtg atgtcctac 
      ggggagagg ccctactgg aattggtcg aatcaggac 
      gtcatcaag agcatagag aaggggtac cgacttccg 
      gcccccatg gactgtcct gaagcgatc taccagctc 
      atgttggac tgttggcag aaggagcgc acccacagg 
      cccacattc caatcgatt gtaaagact ttagataaa 
      ttaatacgt gttccggac acgttgcgg aaaatcgct 
      cagaatcga cacctgcca ccgtgctta aacccatac 
      tttcggcaa gatttgcgt cacgtgtca gaactgccc 
      gcgcgcgag tcgtttttc gattacggc cttccggac 
      gtaatactt ttgcaaaac tacgaaatg tctcagtgg 
      ctttcgcag gaacgtttc aatttgagt tccgatttt 
      ggcagttct gatcagacg ctgttcaca taccaggga 
      gacactcac acgccatca cacacgtac actaaaagg 
      ggcgcttgg gtcactttt agcaacatg taaatatgc 
      ttttgtttg atttttgca tgacttttg tgtttctgc 
      ttcctaatt tttcattaa ta
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