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Gene information
General information
TC006027 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 2529730..2531671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006027
      iBeetle screen iB_04056
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006027 RA
        MKTSALSVL SKINSNVVQ NSRRLFSFN FEMTPASRQ 
        PEAKEETKP EEQPKNFRY NVCQPRTSP VERPNSAGD 
        VIALQKQNV KAAIEEQQL QPSMLPEYF QKTMEKSVL 
        TNPEPEHTE EGRINKGLN PKNPRRFQV SKTYANAIL 
        PLKSVKLAN PFPEPVKMM MSRTVSDDE KAEQMLAEA 
        RSQKQLNEQ IANKSSTFR SFSTEVKRW YSKTVGADP 
        DKCKKMGKG KKGCPKMKL DNCPPPART RCDRFAKHI 
        DCKKQEAPY PAYSEFCHE EIEEKPSSC KMCPWKPEE 
        NPNFKPSKK KFHTSSHPL MVRRSGLEM DLTLNDPLV 
        ETATEENDM LCDLRKPPC ERPKNRRCP EPKTKKKEE 
        KKKKPKPKQ ECAILDNNI LEPIGALLE PEAITLKSK 
        KPKEQGHGS CKIPKFKFP CCDQKVTRT DQDGRCPTK 
        RRKPKVRDC PPDEKSCDT VEVVEEYSL SGSGGKKDK 
        TKCPQIDMT TLREELDDD PCRLKKKGT TKICPGDLL 
        KKKRRKPRK KTSGVIAPV EKIEPQRPT KSV
      CDS sequence
      • >TC006027 RA
        atgaaaacc tcagctctg agcgtactc tccaaaatt 
        aacagcaat gtggtacaa aattctcgt agactcttc 
        tcgtttaat ttcgagatg acaccggca agtcggcaa 
        ccggaagcc aaagaggag acaaaaccg gaagaacaa 
        ccgaaaaac ttccgctac aatgtatgc cagccccgg 
        acgagtccg gtcgagaga cccaactca gccggggac 
        gtcatagcc ctccaaaaa cagaacgtg aaggcagcc 
        atcgaagaa caacaactc caaccctcc atgctcccg 
        gaatacttc caaaaaacg atggagaag agcgtgcta 
        acgaacccg gaaccggaa cacaccgaa gagggccgc 
        atcaacaag ggcctcaac cccaaaaac cccaggcgg 
        ttccaggtg tcgaaaacc tacgccaac gctatcctg 
        ccgctgaaa agcgtcaaa cttgccaac cccttcccg 
        gaacccgtg aagatgatg atgtccagg accgtctcc 
        gacgacgaa aaggccgag caaatgctg gcggaggcc 
        cgcagccag aagcagctc aacgagcaa atcgccaac 
        aaatcctcc accttccgc tcgttctcc accgaagtg 
        aagcgctgg tactccaag acggtgggc gccgacccc 
        gacaagtgc aagaaaatg ggcaagggc aagaaaggc 
        tgccccaaa atgaagctg gacaactgc ccgcccccg 
        gcccgcacg cgctgcgac cgtttcgcc aaacatatt 
        gactgcaaa aagcaggaa gccccttac ccggcttat 
        agcgagttc tgtcacgaa gagatcgag gaaaagccc 
        tcatcctgc aaaatgtgc ccctggaag ccggaggag 
        aaccccaac ttcaagccc tccaagaag aagttccac 
        accagcagc caccccctg atggtgcgc cgcagcggc 
        cttgagatg gacctgacc ctaaacgac cctttggtg 
        gaaacggcc accgaagag aacgacatg ttgtgcgat 
        ttgcgtaag cccccatgt gagcgtccg aaaaaccgg 
        cgttgtccc gaacccaaa accaaaaaa aaggaagag 
        aagaagaag aaaccgaaa ccgaaacag gaatgcgcg 
        attctcgac aacaacatc ctcgagcca attggtgcg 
        ctcctcgaa cccgaggca atcaccctc aagagcaaa 
        aagcccaaa gagcaggga cacggctcg tgcaaaatc 
        cccaaattc aagttcccc tgctgcgac cagaaggtc 
        acccgtacc gaccaggac ggccggtgc cccaccaag 
        cggcgcaag cccaaagtg cgagattgc ccccctgat 
        gaaaagtcc tgcgacacg gtcgaagtc gtggaggag 
        tacagtctg agcggcagc ggggggaag aaggacaag 
        accaagtgc ccccaaatc gacatgacc acgctcagg 
        gaggagctg gatgacgat ccctgccgt ttgaagaag 
        aaggggacc acgaagatt tgtcccggg gatttgttg 
        aagaagaag aggaggaag cccaggaag aagaccagt 
        ggggtgatc gcaccagtt gaaaaaatc gagccccag 
        cggcccacc aagtcggtt taa
      mRNA sequence
      • >TC006027 RA
        ccatgacac cttttattt attcgtgtt gttttgtca 
        tttttttct aaacagttt ttcccatca ccgattttc 
        acaatgaaa acctcagct ctgagcgta ctctccaaa 
        attaacagc aatgtggta caaaattct cgtagactc 
        ttctcgttt aatttcgag atgacaccg gcaagtcgg 
        caaccggaa gccaaagag gagacaaaa ccggaagaa 
        caaccgaaa aacttccgc tacaatgta tgccagccc 
        cggacgagt ccggtcgag agacccaac tcagccggg 
        gacgtcata gccctccaa aaacagaac gtgaaggca 
        gccatcgaa gaacaacaa ctccaaccc tccatgctc 
        ccggaatac ttccaaaaa acgatggag aagagcgtg 
        ctaacgaac ccggaaccg gaacacacc gaagagggc 
        cgcatcaac aagggcctc aaccccaaa aaccccagg 
        cggttccag gtgtcgaaa acctacgcc aacgctatc 
        ctgccgctg aaaagcgtc aaacttgcc aaccccttc 
        ccggaaccc gtgaagatg atgatgtcc aggaccgtc 
        tccgacgac gaaaaggcc gagcaaatg ctggcggag 
        gcccgcagc cagaagcag ctcaacgag caaatcgcc 
        aacaaatcc tccaccttc cgctcgttc tccaccgaa 
        gtgaagcgc tggtactcc aagacggtg ggcgccgac 
        cccgacaag tgcaagaaa atgggcaag ggcaagaaa 
        ggctgcccc aaaatgaag ctggacaac tgcccgccc 
        ccggcccgc acgcgctgc gaccgtttc gccaaacat 
        attgactgc aaaaagcag gaagcccct tacccggct 
        tatagcgag ttctgtcac gaagagatc gaggaaaag 
        ccctcatcc tgcaaaatg tgcccctgg aagccggag 
        gagaacccc aacttcaag ccctccaag aagaagttc 
        cacaccagc agccacccc ctgatggtg cgccgcagc 
        ggccttgag atggacctg accctaaac gaccctttg 
        gtggaaacg gccaccgaa gagaacgac atgttgtgc 
        gatttgcgt aagccccca tgtgagcgt ccgaaaaac 
        cggcgttgt cccgaaccc aaaaccaaa aaaaaggaa 
        gagaagaag aagaaaccg aaaccgaaa caggaatgc 
        gcgattctc gacaacaac atcctcgag ccaattggt 
        gcgctcctc gaacccgag gcaatcacc ctcaagagc 
        aaaaagccc aaagagcag ggacacggc tcgtgcaaa 
        atccccaaa ttcaagttc ccctgctgc gaccagaag 
        gtcacccgt accgaccag gacggccgg tgccccacc 
        aagcggcgc aagcccaaa gtgcgagat tgcccccct 
        gatgaaaag tcctgcgac acggtcgaa gtcgtggag 
        gagtacagt ctgagcggc agcgggggg aagaaggac 
        aagaccaag tgcccccaa atcgacatg accacgctc 
        agggaggag ctggatgac gatccctgc cgtttgaag 
        aagaagggg accacgaag atttgtccc ggggatttg 
        ttgaagaag aagaggagg aagcccagg aagaagacc 
        agtggggtg atcgcacca gttgaaaaa atcgagccc 
        cagcggccc accaagtcg gtttaatgt acgtaatga 
        caaaacaac aaaaataaa aaatcataa aaagt