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Gene information
General information
TC006020 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2918538..2920764
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006020
    iBeetle screen iB_04055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006020 RA
      MSQEETLVK YTAIYRCSD DITNLKIRV KLRQVGDLT 
GEDSDGWEQ KDFAWQQKC FSKSEREYY KDEKNCITD 
LEKKYHSDV GESHTEAHF DLFSYTNDD DFALKQEVN 
DNFRENCDE NGQGYDYGQ SLVAAVRNP IGHNYEFEV 
MYIMADLGE YIDSTWLKQ EQVLCVVKY DRKYKILSI 
NPDFTKSQP YLIRLEGAT IRKYHYFLE NVSAQMPEE 
AHRREKELI RKINGHKTE LLKCLSFKL PAKNKLAVH 
LFMELAWAQ YFEYGPIYV QYFIDLPSD WSPTDQSQL 
QGVTQTCHV TGDEGLAHF GHVFDVTLE YNIEDLGKR 
GIPKCPYIY FEVISKDTW SRYRTEGLT YKALPVAKP 
GNYRFNLQC FRLIPDRIS GQLRRFFIG DCSSYGDLT 
WVGLPKHYD GPVLNKYGT NTVGTGLIS VTVHVVHQS 
QAFLDKYRE NSEENLEIF EKLNSSSLV KSVEQVLQA 
FKRARKNKI AARKFL
    CDS sequence
    • >TC006020 RA
      atgagtcag gaggagact ttggtcaaa tacacggca 
atttacagg tgctcggat gacatcacc aacttgaaa 
ataagagtg aagttgcgc caagtgggg gatttaact 
ggcgaggat tcggacggt tgggagcag aaagacttc 
gcctggcaa cagaaatgc ttcagcaag tcagaaagg 
gagtactac aaggacgaa aaaaactgt atcacagat 
ctggagaaa aagtaccat tcggacgtc ggggaatca 
cacactgag gcgcatttt gacctgttt tcgtacacc 
aatgacgac gattttgcc ctcaaacag gaagttaat 
gacaatttc cgcgaaaat tgcgacgaa aatggccag 
gggtacgac tacggccag tcacttgtg gcagcagtg 
cgaaatccc attggtcat aattacgag tttgaggtc 
atgtacatc atggcggac ctcggcgaa tacatcgat 
tcgacttgg ctcaagcag gaacaagtc ctgtgtgtc 
gttaaatac gaccggaag tacaaaata ttatcaatt 
aaccccgac tttaccaaa tcccagccg tatttaatc 
cgacttgaa ggcgccaca attcgcaaa tatcactat 
ttccttgag aacgtttcg gcccaaatg cctgaggaa 
gcccaccgt cgcgaaaaa gaattaatc cgaaaaata 
aatggccac aaaaccgag cttttgaaa tgtttgagt 
ttcaaactt cccgccaaa aacaaactg gccgttcat 
ttgttcatg gaactcgcg tgggcgcaa tatttcgag 
tatggacca atctacgtc cagtatttc atcgacttg 
ccgtcggat tggtccccg actgaccaa tcgcaactg 
cagggcgtg acgcaaacg tgtcacgtg actggcgat 
gagggtttg gcccatttc gggcacgtt ttcgacgtt 
acgttagag tacaatatc gaggatttg ggcaaaagg 
ggaatcccc aagtgccct tatatctac tttgaggtt 
atttcgaag gacacttgg agtcggtat aggactgaa 
gggctgact tataaggcc ttgcccgtt gccaagccg 
gggaattac cggtttaac ctacaatgt ttccggtta 
atccccgac cggatttcc ggtcagtta cgccggttt 
tttattggc gattgttca agctatggt gacttgact 
tgggtggga ttaccgaaa cattacgat gggcctgtt 
ttaaataaa tatgggact aacacggtt ggtaccggt 
ctgatcagc gtgaccgtt cacgtcgtc catcaaagc 
caggcgttt ctggacaaa tacagagaa aattccgag 
gaaaatttg gaaattttc gaaaaattg aactcgtca 
agtttggtc aaaagcgtg gagcaggtg ctccaagcg 
ttcaaaagg gcccgaaaa aacaagatt gcggcccgg 
aaattttta taa
    mRNA sequence
    • >TC006020 RA
      aaatgagtc aggaggaga ctttggtca aatacacgg 
caatttaca ggtgctcgg atgacatca ccaacttga 
aaataagag tgaagttgc gccaagtgg gggatttaa 
ctggcgagg attcggacg gttgggagc agaaagact 
tcgcctggc aacagaaat gcttcagca agtcagaaa 
gggagtact acaaggacg aaaaaaact gtatcacag 
atctggaga aaaagtacc attcggacg tcggggaat 
cacacactg aggcgcatt ttgacctgt tttcgtaca 
ccaatgacg acgattttg ccctcaaac aggaagtta 
atgacaatt tccgcgaaa attgcgacg aaaatggcc 
aggggtacg actacggcc agtcacttg tggcagcag 
tgcgaaatc ccattggtc ataattacg agtttgagg 
tcatgtaca tcatggcgg acctcggcg aatacatcg 
attcgactt ggctcaagc aggaacaag tcctgtgtg 
tcgttaaat acgaccgga agtacaaaa tattatcaa 
ttaaccccg actttacca aatcccagc cgtatttaa 
tccgacttg aaggcgcca caattcgca aatatcact 
atttccttg agaacgttt cggcccaaa tgcctgagg 
aagcccacc gtcgcgaaa aagaattaa tccgaaaaa 
taaatggcc acaaaaccg agcttttga aatgtttga 
gtttcaaac ttcccgcca aaaacaaac tggccgttc 
atttgttca tggaactcg cgtgggcgc aatatttcg 
agtatggac caatctacg tccagtatt tcatcgact 
tgccgtcgg attggtccc cgactgacc aatcgcaac 
tgcagggcg tgacgcaaa cgtgtcacg tgactggcg 
atgagggtt tggcccatt tcgggcacg ttttcgacg 
ttacgttag agtacaata tcgaggatt tgggcaaaa 
ggggaatcc ccaagtgcc cttatatct actttgagg 
ttatttcga aggacactt ggagtcggt ataggactg 
aagggctga cttataagg ccttgcccg ttgccaagc 
cggggaatt accggttta acctacaat gtttccggt 
taatccccg accggattt ccggtcagt tacgccggt 
tttttattg gcgattgtt caagctatg gtgacttga 
cttgggtgg gattaccga aacattacg atgggcctg 
ttttaaata aatatggga ctaacacgg ttggtaccg 
gtctgatca gcgtgaccg ttcacgtcg tccatcaaa 
gccaggcgt ttctggaca aatacagag aaaattccg 
aggaaaatt tggaaattt tcgaaaaat tgaactcgt 
caagtttgg tcaaaagcg tggagcagg tgctccaag 
cgttcaaaa gggcccgaa aaaacaaga ttgcggccc 
ggaaatttt tataattgt tgttttatt ttgagaatc 
aataaacgt ggcaacgcc gc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY