General information | |||
TC005999 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 3803158..3807733 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC005999 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04054 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
antenna unilateral shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna unilateral not present - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna unilateral partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC005999 RA
MSVPPNAAP PEPINTEST GWKIFSAFI HDTPLSVPE EDLWTYVSD ELKLDTQGI PYECREQLP LILVAFMNI WSGWHFKTD FREGKFPKE HYYHRFHKY MRHKLHTAI RGQLAVALK AVKDQQQRT AALVQAEPE NKIDSDFSP FTIHVQCPA NMLNTIVFS NVGDDIVMK QHLGGEWEE LTTTQLLNW PHVEAIFRT KDICFVRRQ RTEYLRYLN FLPHESVKH SESVIDNVE ILPNYGNQR RCFSYNFFK AYFNTYLEF LEKLVAPVK FNEIQDILT QFLEDVKYI NVNNSMVTN TSVSFRLSP VPFIRNKKL VKNSDNKLD SRVGYCEII TPQIELILV YCKAEQTDT PYLSFDFIE ELKNSDKTE AVIMPSIVY RCTFCSVRF TSHDSKKML LQHLKITHK MEQQVRCTY CKKHFNVPS LAANRWSHK CQPSQNSSV SRSNNAVSS TSGTSSEQT SITIN
- >TC005999 RA
atgagtgtg cccccaaat gcagcccct ccggagccc atcaacacc gagtccact ggctggaag attttttct gcttttatt cacgacacc cctctaagt gtgcccgag gaagacctg tggacttac gtttctgac gaactgaag ctcgatact caagggatt ccgtacgaa tgcagggaa caattgcct ttaatctta gtggcgttt atgaatatc tggtccggg tggcacttt aagaccgat tttcgggag gggaagttt cccaaggag cattattac catcgtttc cacaagtat atgagacac aaattgcac acagccatt cgggggcag ctagctgtt gctctcaaa gccgtaaaa gaccagcag cagagaacg gctgcgcta gttcaggcg gagcctgag aataaaatt gattcggat ttttcgccc ttcactatt cacgtgcag tgtccggca aatatgttg aatactatt gttttctca aatgttgga gatgatatt gtgatgaaa cagcacctt gggggcgaa tgggaggaa ttaacaaca acccaactt ttgaactgg cctcacgtt gaggcgatt tttcgtacg aaggatatc tgtttcgtg cgacggcaa cgcactgaa tatttgcgt tatttaaat ttcttaccg cacgaatcg gtcaaacac tcagaatcc gtcattgac aacgtggaa attttgccg aattacgga aaccaaagg cggtgtttc agctacaac ttcttcaag gcgtatttt aacacttat ctcgaattt ttggagaaa ttggtcgct ccagtaaaa ttcaacgag atccaagac atcctcact cagttcctc gaggacgtg aaatacatc aacgtgaat aacagcatg gtgaccaac acttcagtc tcattcaga ttatctcca gtgccattc attcgcaat aaaaaatta gtgaaaaac tccgataat aaactagac tcgagggtg ggatattgt gaaataata acgccacaa attgagttg attttggtg tattgcaag gccgaacag acggatacg ccatatctc agttttgat ttcatcgaa gaactgaaa aattcggac aaaactgag gctgtcatt atgccgtcc atagtgtac aggtgtacg ttttgcagt gttcgattt acttcgcac gattcgaaa aaaatgtta ctgcagcat ttaaaaatt acacacaag atggaacaa caagttcgg tgtacttac tgtaagaaa cattttaat gtgcccagt ttggcggca aatcggtgg tcacataag tgtcagcca agtcaaaat agttcggtt agtcgttct aataatgcg gtgtcaagt acttcaggc acttcaagt gagcaaacg agtattaca attaattaa
- >TC005999 RA
actgtaatt tttttaaag caagtcaaa aatggcggt gtttggcca aaagtgagg ttaggtcaa aaaaaaaca aaaagtttt tgcaatgag tgtgccccc aaatgcagc ccctccgga gcccatcaa caccgagtc cactggctg gaagatttt ttctgcttt tattcacga cacccctct aagtgtgcc cgaggaaga cctgtggac ttacgtttc tgacgaact gaagctcga tactcaagg gattccgta cgaatgcag ggaacaatt gcctttaat cttagtggc gtttatgaa tatctggtc cgggtggca ctttaagac cgattttcg ggaggggaa gtttcccaa ggagcatta ttaccatcg tttccacaa gtatatgag acacaaatt gcacacagc cattcgggg gcagctagc tgttgctct caaagccgt aaaagacca gcagcagag aacggctgc gctagttca ggcggagcc tgagaataa aattgattc ggatttttc gcccttcac tattcacgt gcagtgtcc ggcaaatat gttgaatac tattgtttt ctcaaatgt tggagatga tattgtgat gaaacagca ccttggggg cgaatggga ggaattaac aacaaccca acttttgaa ctggcctca cgttgaggc gatttttcg tacgaagga tatctgttt cgtgcgacg gcaacgcac tgaatattt gcgttattt aaatttctt accgcacga atcggtcaa acactcaga atccgtcat tgacaacgt ggaaatttt gccgaatta cggaaacca aaggcggtg tttcagcta caacttctt caaggcgta ttttaacac ttatctcga atttttgga gaaattggt cgctccagt aaaattcaa cgagatcca agacatcct cactcagtt cctcgagga cgtgaaata catcaacgt gaataacag catggtgac caacacttc agtctcatt cagattatc tccagtgcc attcattcg caataaaaa attagtgaa aaactccga taataaact agactcgag ggtgggata ttgtgaaat aataacgcc acaaattga gttgatttt ggtgtattg caaggccga acagacgga tacgccata tctcagttt tgatttcat cgaagaact gaaaaattc ggacaaaac tgaggctgt cattatgcc gtccatagt gtacaggtg tacgttttg cagtgttcg atttacttc gcacgattc gaaaaaaat gttactgca gcatttaaa aattacaca caagatgga acaacaagt tcggtgtac ttactgtaa gaaacattt taatgtgcc cagtttggc ggcaaatcg gtggtcaca taagtgtca gccaagtca aaatagttc ggttagtcg ttctaataa tgcggtgtc aagtacttc aggcacttc aagtgagca aacgagtat tacaattaa ttaaaaagt tattgtttg aaggaaaag agaaatgta ttttgtatt gtgtgatga tttagtctt ggttgataa ttttgccac ttcattttt tacttcggt tgtactcat acaaggtga atcagcaaa aaatatttt tactaattt tttacgtgt gtttttcca aaacatatc ttttaagct tcgttagaa atcttcata tttcgatca attaataac tgtagaaaa attcacttg cacagagaa acatataaa gtacttaat agcacttaa tagcagcaa tagcactta attcggtgt aatcattgt ttttttaga aaaatgcta ttagcagac tcacctgta tgccaccag gttaatgta tttattatt ttttaactt gtatttaag tatagtatt tgtttaatt ttacactta gtcaagaat aagaacatc gaagtaagg atttcacgt catgcgttt ttttgttat tttctatag ctattaatt taggttctg ttatttaga aattttcaa ttttgaaaa ttatttgtg ttatacaat ggcgagcaa aaaatttcg ggcaccttt gacatttca ctgacataa tcctgtgta attccctat cctttttga caatgaagt gacaaaagt gcccgaaat tttttgctc gccgttata cgtgaatga taataaaca attatgcat ttt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
