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Gene information
General information
TC005999 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3497736..3502311
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005999
      iBeetle screen iB_04054
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      antenna unilateral shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      antenna unilateral not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      antenna unilateral partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005999 RA
        MSVPPNAAP PEPINTEST GWKIFSAFI HDTPLSVPE 
        EDLWTYVSD ELKLDTQGI PYECREQLP LILVAFMNI 
        WSGWHFKTD FREGKFPKE HYYHRFHKY MRHKLHTAI 
        RGQLAVALK AVKDQQQRT AALVQAEPE NKIDSDFSP 
        FTIHVQCPA NMLNTIVFS NVGDDIVMK QHLGGEWEE 
        LTTTQLLNW PHVEAIFRT KDICFVRRQ RTEYLRYLN 
        FLPHESVKH SESVIDNVE ILPNYGNQR RCFSYNFFK 
        AYFNTYLEF LEKLVAPVK FNEIQDILT QFLEDVKYI 
        NVNNSMVTN TSVSFRLSP VPFIRNKKL VKNSDNKLD 
        SRVGYCEII TPQIELILV YCKAEQTDT PYLSFDFIE 
        ELKNSDKTE AVIMPSIVY RCTFCSVRF TSHDSKKML 
        LQHLKITHK MEQQVRCTY CKKHFNVPS LAANRWSHK 
        CQPSQNSSV SRSNNAVSS TSGTSSEQT SITIN
      CDS sequence
      • >TC005999 RA
        atgagtgtg cccccaaat gcagcccct ccggagccc 
        atcaacacc gagtccact ggctggaag attttttct 
        gcttttatt cacgacacc cctctaagt gtgcccgag 
        gaagacctg tggacttac gtttctgac gaactgaag 
        ctcgatact caagggatt ccgtacgaa tgcagggaa 
        caattgcct ttaatctta gtggcgttt atgaatatc 
        tggtccggg tggcacttt aagaccgat tttcgggag 
        gggaagttt cccaaggag cattattac catcgtttc 
        cacaagtat atgagacac aaattgcac acagccatt 
        cgggggcag ctagctgtt gctctcaaa gccgtaaaa 
        gaccagcag cagagaacg gctgcgcta gttcaggcg 
        gagcctgag aataaaatt gattcggat ttttcgccc 
        ttcactatt cacgtgcag tgtccggca aatatgttg 
        aatactatt gttttctca aatgttgga gatgatatt 
        gtgatgaaa cagcacctt gggggcgaa tgggaggaa 
        ttaacaaca acccaactt ttgaactgg cctcacgtt 
        gaggcgatt tttcgtacg aaggatatc tgtttcgtg 
        cgacggcaa cgcactgaa tatttgcgt tatttaaat 
        ttcttaccg cacgaatcg gtcaaacac tcagaatcc 
        gtcattgac aacgtggaa attttgccg aattacgga 
        aaccaaagg cggtgtttc agctacaac ttcttcaag 
        gcgtatttt aacacttat ctcgaattt ttggagaaa 
        ttggtcgct ccagtaaaa ttcaacgag atccaagac 
        atcctcact cagttcctc gaggacgtg aaatacatc 
        aacgtgaat aacagcatg gtgaccaac acttcagtc 
        tcattcaga ttatctcca gtgccattc attcgcaat 
        aaaaaatta gtgaaaaac tccgataat aaactagac 
        tcgagggtg ggatattgt gaaataata acgccacaa 
        attgagttg attttggtg tattgcaag gccgaacag 
        acggatacg ccatatctc agttttgat ttcatcgaa 
        gaactgaaa aattcggac aaaactgag gctgtcatt 
        atgccgtcc atagtgtac aggtgtacg ttttgcagt 
        gttcgattt acttcgcac gattcgaaa aaaatgtta 
        ctgcagcat ttaaaaatt acacacaag atggaacaa 
        caagttcgg tgtacttac tgtaagaaa cattttaat 
        gtgcccagt ttggcggca aatcggtgg tcacataag 
        tgtcagcca agtcaaaat agttcggtt agtcgttct 
        aataatgcg gtgtcaagt acttcaggc acttcaagt 
        gagcaaacg agtattaca attaattaa
      mRNA sequence
      • >TC005999 RA
        actgtaatt tttttaaag caagtcaaa aatggcggt 
        gtttggcca aaagtgagg ttaggtcaa aaaaaaaca 
        aaaagtttt tgcaatgag tgtgccccc aaatgcagc 
        ccctccgga gcccatcaa caccgagtc cactggctg 
        gaagatttt ttctgcttt tattcacga cacccctct 
        aagtgtgcc cgaggaaga cctgtggac ttacgtttc 
        tgacgaact gaagctcga tactcaagg gattccgta 
        cgaatgcag ggaacaatt gcctttaat cttagtggc 
        gtttatgaa tatctggtc cgggtggca ctttaagac 
        cgattttcg ggaggggaa gtttcccaa ggagcatta 
        ttaccatcg tttccacaa gtatatgag acacaaatt 
        gcacacagc cattcgggg gcagctagc tgttgctct 
        caaagccgt aaaagacca gcagcagag aacggctgc 
        gctagttca ggcggagcc tgagaataa aattgattc 
        ggatttttc gcccttcac tattcacgt gcagtgtcc 
        ggcaaatat gttgaatac tattgtttt ctcaaatgt 
        tggagatga tattgtgat gaaacagca ccttggggg 
        cgaatggga ggaattaac aacaaccca acttttgaa 
        ctggcctca cgttgaggc gatttttcg tacgaagga 
        tatctgttt cgtgcgacg gcaacgcac tgaatattt 
        gcgttattt aaatttctt accgcacga atcggtcaa 
        acactcaga atccgtcat tgacaacgt ggaaatttt 
        gccgaatta cggaaacca aaggcggtg tttcagcta 
        caacttctt caaggcgta ttttaacac ttatctcga 
        atttttgga gaaattggt cgctccagt aaaattcaa 
        cgagatcca agacatcct cactcagtt cctcgagga 
        cgtgaaata catcaacgt gaataacag catggtgac 
        caacacttc agtctcatt cagattatc tccagtgcc 
        attcattcg caataaaaa attagtgaa aaactccga 
        taataaact agactcgag ggtgggata ttgtgaaat 
        aataacgcc acaaattga gttgatttt ggtgtattg 
        caaggccga acagacgga tacgccata tctcagttt 
        tgatttcat cgaagaact gaaaaattc ggacaaaac 
        tgaggctgt cattatgcc gtccatagt gtacaggtg 
        tacgttttg cagtgttcg atttacttc gcacgattc 
        gaaaaaaat gttactgca gcatttaaa aattacaca 
        caagatgga acaacaagt tcggtgtac ttactgtaa 
        gaaacattt taatgtgcc cagtttggc ggcaaatcg 
        gtggtcaca taagtgtca gccaagtca aaatagttc 
        ggttagtcg ttctaataa tgcggtgtc aagtacttc 
        aggcacttc aagtgagca aacgagtat tacaattaa 
        ttaaaaagt tattgtttg aaggaaaag agaaatgta 
        ttttgtatt gtgtgatga tttagtctt ggttgataa 
        ttttgccac ttcattttt tacttcggt tgtactcat 
        acaaggtga atcagcaaa aaatatttt tactaattt 
        tttacgtgt gtttttcca aaacatatc ttttaagct 
        tcgttagaa atcttcata tttcgatca attaataac 
        tgtagaaaa attcacttg cacagagaa acatataaa 
        gtacttaat agcacttaa tagcagcaa tagcactta 
        attcggtgt aatcattgt ttttttaga aaaatgcta 
        ttagcagac tcacctgta tgccaccag gttaatgta 
        tttattatt ttttaactt gtatttaag tatagtatt 
        tgtttaatt ttacactta gtcaagaat aagaacatc 
        gaagtaagg atttcacgt catgcgttt ttttgttat 
        tttctatag ctattaatt taggttctg ttatttaga 
        aattttcaa ttttgaaaa ttatttgtg ttatacaat 
        ggcgagcaa aaaatttcg ggcaccttt gacatttca 
        ctgacataa tcctgtgta attccctat cctttttga 
        caatgaagt gacaaaagt gcccgaaat tttttgctc 
        gccgttata cgtgaatga taataaaca attatgcat 
        ttt