Status
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Gene information
General information
TC005995 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3907770..3973753
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005995
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04052

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005995 RA
      MPHTILRPH HPVNGYAHH YTQNGHVKN NGHGGMNGT 
      GRPWMNGHA TKPEDKQST LSRYCTTER QRHSSNYQA 
      IRRACRERG SLFEDPDFP AGPRALYHH KKPSLHPIV 
      WMRPHEMCQ RPRFISDNN NSGEMRFAV EAGELGDQW 
      LLAAVASLA LTPRFLDRI VPPDQGFDN SHNYCGVFR 
      FRFWHFGEW KDVIVDDKL PTFKGRLVH LHCSNTTEF 
      WAALLEKAY AKFYGCYEN LAQGSTTRA LQDLTGGIV 
      QSFGLTHQD RFLTFQVLN SAVPRSSLL IATVTQEKE 
      NKRQLRLRN GLITQHAYS VTGLARVRG PQGETPLVR 
      LRNPWARGE WTGAWSERS WEWDGLSTR DKELLSVRV 
      ANDGEFWMS FEDFSKHFT QLDLVHIGP DDWMMESAL 
      HSKQPWRAV LARRRWRAG YNAGGGPSY VDTTSMNPQ 
      FHVQIPRTS SNKCHVVVS ITQSYETSV ADSKKKKPL 
      YAIGFAVYE VPHSMQRLT PHFVADKLK PLDVTNHSI 
      AREVVTFFT LPPGDYIVV PQTNIPNLD GKFLLRVFT 
      DEQSNIWEV NEDNMIIRN ISSELMEET GPVTPEGKS 
      ILSKLLTKF PPEVDASQL QKILRAHWK SYLSEKPSL 
      ELCKSLIML RDYNISGRI NLMDIPVLM HMLQFWRCA 
      FQKFERGHG NGKTSSYHL RALLWEAGC TVSNKVLEC 
      LVLRFAKNR ILTTENYVM ALVRLHLAH ERYHNIDTK 
      MKSNPLSLE EMILMTIYS
    • >TC005995 RB
      MPHTILRPH HPVNGYAHH YTQNGHVKN NGHGGMNGT 
      GRPWMNGHA TKPEDKQST LSR
    • >TC005995 RC
      MQEYNMDNN FPRYCTTER QRHSSNYQA IRRACRERG 
      SLFEDPDFP AGPRALYHH KKPSLHPIV WMRPHEMCQ 
      RPRFISDNN NSGEMRFAV EAGELGDQW LLAAVASLA 
      LTPRFLDRI VPPDQGFDN SHNYCGVFR FRFWHFGEW 
      KDVIVDDKL PTFKGRLVH LHCSNTTEF WAALLEKAY 
      AKFYGCYEN LAQGSTTRA LQDLTGGIV QSFGLTHQD 
      RFLTFQVLN SAVPRSSLL IATVTQEKE NKRQLRLRN 
      GLITQHAYS VTGLARVRG PQGETPLVR LRNPWARGE 
      WTGAWSERS WEWDGLSTR DKELLSVRV ANDGEFWMS 
      FEDFSKHFT QLDLVHIGP DDWMMESAL HSKQPWRAV 
      LARRRWRAG YNAGGGPSY VDTTSMNPQ FHVQIPRTS 
      SNKCHVVVS ITQSYETSV ADSKKKKPL YAIGFAVYE 
      VPHSMQRLT PHFVADKLK PLDVTNHSI AREVVTFFT 
      LPPGDYIVV PQTNIPNLD GKFLLRVFT DEQSNIWEV 
      NEDNMIIRN ISSELMEET GPVTPEGKS ILSKLLTKF 
      PPEVDASQL QKILRAHWK SYLSEKPSL ELCKSLIML 
      RDYNISGRI NLMDIPVLM HMLQFWRCA FQKFERGHG 
      NGKTSSYHL RALLWEAGC TVSNKVLEC LVLRFAKNR 
      ILTTENYVM ALVRLHLAH ERYHNIDTK MKSNPLSLE 
      EMILMTIYS
    CDS sequence
    • >TC005995 RA
      atgcctcac acgatctta cggccccat catcccgtc 
      aatggctat gcccatcat tacacccag aatggacat 
      gtcaaaaat aatgggcat ggtggcatg aacggtacc 
      ggaagaccg tggatgaac ggacacgct acgaaaccg 
      gaggacaag cagtcgact ttatcgaga tattgcacc 
      actgaacgg cagcgccac tcttccaac taccaagca 
      atccgtcga gcatgtcgc gagagaggc tcccttttc 
      gaggacccg gattttccg gcgggccct cgcgccctc 
      taccaccac aagaagccg tccctgcac ccgatcgtg 
      tggatgagg ccccacgaa atgtgccag aggccgcga 
      ttcatcagc gacaacaac aactccgga gagatgcga 
      ttcgctgtc gaagccgga gaattgggc gaccaatgg 
      cttttggca gctgtggcc tctctggct ctaacacct 
      cgattcctg gaccggatc gtgcctccg gatcaagga 
      ttcgacaac tcgcataac tactgcgga gttttcagg 
      tttcgattc tggcacttc ggcgaatgg aaggacgtg 
      atagtggac gacaagctc cccactttc aaggggcgc 
      ctcgtccac ttgcactgc tccaacacc acggaattc 
      tgggcagct ttgctcgaa aaagcctac gccaaattc 
      tacggctgt tacgagaat ctggcgcaa ggttcaacc 
      actcgcgcc ctccaagac ctgaccggt ggcatcgtc 
      caaagtttc ggcctcact catcaggac cgctttttg 
      acatttcaa gtgttaaac agcgctgtt cccagatct 
      agtctactg attgcgacc gtcactcag gaaaaagag 
      aacaaaaga cagctgagg ctacgcaat gggctgatc 
      acccagcac gcgtacagt gtaacaggg ttggcccga 
      gtgagaggg ccgcagggc gaaacaccg ttggtaaga 
      ctgagaaac ccatgggcg cggggggag tggacgggg 
      gcgtggagt gagcggtcg tgggagtgg gacgggctg 
      tctaccagg gacaaggag ttgctgagt gtgcgcgta 
      gcgaacgat ggggagttc tggatgtcg tttgaggac 
      ttttccaag catttcacg cagttggat ttggtccat 
      attgggcct gatgattgg atgatggaa agtgcctta 
      cattcgaag caaccatgg agggcggtt ctggccagg 
      aggcgctgg agggcgggg tataacgct gggggcggg 
      cccagctat gttgacacg acgtcaatg aatccacaa 
      tttcatgtc caaattccg aggacttca agcaataaa 
      tgtcacgtt gtggtgtcc ataacgcaa agttatgaa 
      actagcgtt gctgatagc aaaaagaag aagcctctc 
      tatgccata gggtttgcc gtttatgaa gtacctcat 
      tcgatgcaa aggctaact cctcacttc gttgcggac 
      aagttaaaa cctctagat gtcactaac cactcaatt 
      gccagagaa gtcgtaact ttctttact ttacctcct 
      ggtgactac attgtagta ccacaaacc aatatcccc 
      aacctggac ggcaaattc cttttaaga gtttttacg 
      gacgaacag agcaacatt tgggaagtc aacgaagat 
      aacatgatt ataagaaac atttcgtca gaactgatg 
      gaagaaaca ggacctgtt acgcccgaa ggcaagtca 
      attttgtca aaactcctc acaaaattc cccccagaa 
      gttgatgct agtcagtta caaaaaatt ctgagggcc 
      cactggaag tcgtacttg tccgaaaaa ccgtcgttg 
      gaactgtgc aaaagcttg atcatgttg cgtgattac 
      aacattagt ggccgcatt aacttaatg gatattccc 
      gtgttgatg catatgctt caattttgg agatgcgct 
      ttccaaaaa ttcgaacga ggtcacggc aatggtaaa 
      acttcaagt tatcatctt cgagctctg ttatgggag 
      gccgggtgt actgttagc aataaagtt ctagaatgt 
      ttggtgctt aggtttgcc aaaaatcgg attctgaca 
      acagagaat tacgtgatg gccttagta cgcttgcat 
      ttagcgcac gaacggtac cataacatt gatacgaag 
      atgaagagc aatcctttg tctttagaa gaaatgatc 
      ttgatgact atttattca tga
    • >TC005995 RB
      atgcctcac acgatctta cggccccat catcccgtc 
      aatggctat gcccatcat tacacccag aatggacat 
      gtcaaaaat aatgggcat ggtggcatg aacggtacc 
      ggaagaccg tggatgaac ggacacgct acgaaaccg 
      gaggacaag cagtcgact ttatcgagg taa
    • >TC005995 RC
      atgcaggaa tataacatg gacaataat tttcccaga 
      tattgcacc actgaacgg cagcgccac tcttccaac 
      taccaagca atccgtcga gcatgtcgc gagagaggc 
      tcccttttc gaggacccg gattttccg gcgggccct 
      cgcgccctc taccaccac aagaagccg tccctgcac 
      ccgatcgtg tggatgagg ccccacgaa atgtgccag 
      aggccgcga ttcatcagc gacaacaac aactccgga 
      gagatgcga ttcgctgtc gaagccgga gaattgggc 
      gaccaatgg cttttggca gctgtggcc tctctggct 
      ctaacacct cgattcctg gaccggatc gtgcctccg 
      gatcaagga ttcgacaac tcgcataac tactgcgga 
      gttttcagg tttcgattc tggcacttc ggcgaatgg 
      aaggacgtg atagtggac gacaagctc cccactttc 
      aaggggcgc ctcgtccac ttgcactgc tccaacacc 
      acggaattc tgggcagct ttgctcgaa aaagcctac 
      gccaaattc tacggctgt tacgagaat ctggcgcaa 
      ggttcaacc actcgcgcc ctccaagac ctgaccggt 
      ggcatcgtc caaagtttc ggcctcact catcaggac 
      cgctttttg acatttcaa gtgttaaac agcgctgtt 
      cccagatct agtctactg attgcgacc gtcactcag 
      gaaaaagag aacaaaaga cagctgagg ctacgcaat 
      gggctgatc acccagcac gcgtacagt gtaacaggg 
      ttggcccga gtgagaggg ccgcagggc gaaacaccg 
      ttggtaaga ctgagaaac ccatgggcg cggggggag 
      tggacgggg gcgtggagt gagcggtcg tgggagtgg 
      gacgggctg tctaccagg gacaaggag ttgctgagt 
      gtgcgcgta gcgaacgat ggggagttc tggatgtcg 
      tttgaggac ttttccaag catttcacg cagttggat 
      ttggtccat attgggcct gatgattgg atgatggaa 
      agtgcctta cattcgaag caaccatgg agggcggtt 
      ctggccagg aggcgctgg agggcgggg tataacgct 
      gggggcggg cccagctat gttgacacg acgtcaatg 
      aatccacaa tttcatgtc caaattccg aggacttca 
      agcaataaa tgtcacgtt gtggtgtcc ataacgcaa 
      agttatgaa actagcgtt gctgatagc aaaaagaag 
      aagcctctc tatgccata gggtttgcc gtttatgaa 
      gtacctcat tcgatgcaa aggctaact cctcacttc 
      gttgcggac aagttaaaa cctctagat gtcactaac 
      cactcaatt gccagagaa gtcgtaact ttctttact 
      ttacctcct ggtgactac attgtagta ccacaaacc 
      aatatcccc aacctggac ggcaaattc cttttaaga 
      gtttttacg gacgaacag agcaacatt tgggaagtc 
      aacgaagat aacatgatt ataagaaac atttcgtca 
      gaactgatg gaagaaaca ggacctgtt acgcccgaa 
      ggcaagtca attttgtca aaactcctc acaaaattc 
      cccccagaa gttgatgct agtcagtta caaaaaatt 
      ctgagggcc cactggaag tcgtacttg tccgaaaaa 
      ccgtcgttg gaactgtgc aaaagcttg atcatgttg 
      cgtgattac aacattagt ggccgcatt aacttaatg 
      gatattccc gtgttgatg catatgctt caattttgg 
      agatgcgct ttccaaaaa ttcgaacga ggtcacggc 
      aatggtaaa acttcaagt tatcatctt cgagctctg 
      ttatgggag gccgggtgt actgttagc aataaagtt 
      ctagaatgt ttggtgctt aggtttgcc aaaaatcgg 
      attctgaca acagagaat tacgtgatg gccttagta 
      cgcttgcat ttagcgcac gaacggtac cataacatt 
      gatacgaag atgaagagc aatcctttg tctttagaa 
      gaaatgatc ttgatgact atttattca tga
    mRNA sequence
    • >TC005995 RA
      cctgtaacc accgttcaa caggtgtcc gacaatttc 
      cggctctag tcgaggaaa ctcggacac gcatattct 
      cctagacta ggtatagta tagtcggtg cattttctt 
      tcatccagt gatgcttcg tccgtttga cattttaca 
      agtcggcaa cttaacggt gctacaacg tgcgtgttt 
      caacatcgg agtatagtg tttttttta tttgaaaac 
      tgtgttggg ggaatcacg cttcaaata attcaatta 
      cgagatgat ttcataatg cgagatgcc tcacacgat 
      cttacggcc ccatcatcc cgtcaatgg ctatgccca 
      tcattacac ccagaatgg acatgtcaa aaataatgg 
      gcatggtgg catgaacgg taccggaag accgtggat 
      gaacggaca cgctacgaa accggagga caagcagtc 
      gactttatc gagatattg caccactga acggcagcg 
      ccactcttc caactacca agcaatccg tcgagcatg 
      tcgcgagag aggctccct tttcgagga cccggattt 
      tccggcggg ccctcgcgc cctctacca ccacaagaa 
      gccgtccct gcacccgat cgtgtggat gaggcccca 
      cgaaatgtg ccagaggcc gcgattcat cagcgacaa 
      caacaactc cggagagat gcgattcgc tgtcgaagc 
      cggagaatt gggcgacca atggctttt ggcagctgt 
      ggcctctct ggctctaac acctcgatt cctggaccg 
      gatcgtgcc tccggatca aggattcga caactcgca 
      taactactg cggagtttt caggtttcg attctggca 
      cttcggcga atggaagga cgtgatagt ggacgacaa 
      gctccccac tttcaaggg gcgcctcgt ccacttgca 
      ctgctccaa caccacgga attctgggc agctttgct 
      cgaaaaagc ctacgccaa attctacgg ctgttacga 
      gaatctggc gcaaggttc aaccactcg cgccctcca 
      agacctgac cggtggcat cgtccaaag tttcggcct 
      cactcatca ggaccgctt tttgacatt tcaagtgtt 
      aaacagcgc tgttcccag atctagtct actgattgc 
      gaccgtcac tcaggaaaa agagaacaa aagacagct 
      gaggctacg caatgggct gatcaccca gcacgcgta 
      cagtgtaac agggttggc ccgagtgag agggccgca 
      gggcgaaac accgttggt aagactgag aaacccatg 
      ggcgcgggg ggagtggac gggggcgtg gagtgagcg 
      gtcgtggga gtgggacgg gctgtctac cagggacaa 
      ggagttgct gagtgtgcg cgtagcgaa cgatgggga 
      gttctggat gtcgtttga ggacttttc caagcattt 
      cacgcagtt ggatttggt ccatattgg gcctgatga 
      ttggatgat ggaaagtgc cttacattc gaagcaacc 
      atggagggc ggttctggc caggaggcg ctggagggc 
      ggggtataa cgctggggg cgggcccag ctatgttga 
      cacgacgtc aatgaatcc acaatttca tgtccaaat 
      tccgaggac ttcaagcaa taaatgtca cgttgtggt 
      gtccataac gcaaagtta tgaaactag cgttgctga 
      tagcaaaaa gaagaagcc tctctatgc catagggtt 
      tgccgttta tgaagtacc tcattcgat gcaaaggct 
      aactcctca cttcgttgc ggacaagtt aaaacctct 
      agatgtcac taaccactc aattgccag agaagtcgt 
      aactttctt tactttacc tcctggtga ctacattgt 
      agtaccaca aaccaatat ccccaacct ggacggcaa 
      attcctttt aagagtttt tacggacga acagagcaa 
      catttggga agtcaacga agataacat gattataag 
      aaacatttc gtcagaact gatggaaga aacaggacc 
      tgttacgcc cgaaggcaa gtcaatttt gtcaaaact 
      cctcacaaa attcccccc agaagttga tgctagtca 
      gttacaaaa aattctgag ggcccactg gaagtcgta 
      cttgtccga aaaaccgtc gttggaact gtgcaaaag 
      cttgatcat gttgcgtga ttacaacat tagtggccg 
      cattaactt aatggatat tcccgtgtt gatgcatat 
      gcttcaatt ttggagatg cgctttcca aaaattcga 
      acgaggtca cggcaatgg taaaacttc aagttatca 
      tcttcgagc tctgttatg ggaggccgg gtgtactgt 
      tagcaataa agttctaga atgtttggt gcttaggtt 
      tgccaaaaa tcggattct gacaacaga gaattacgt 
      gatggcctt agtacgctt gcatttagc gcacgaacg 
      gtaccataa cattgatac gaagatgaa gagcaatcc 
      tttgtcttt agaagaaat gatcttgat gactattta 
      ttcatgata gctttccgg tgatgtgtc tagaagcca 
      aagaatctc tccctccag tctgactgt aaataaaat 
      gtgcaaata ttc
    • >TC005995 RB
      cctgtaacc accgttcaa caggtgtcc gacaatttc 
      cggctctag tcgaggaaa ctcggacac gcatattct 
      cctagacta ggtatagta tagtcggtg cattttctt 
      tcatccagt gatgcttcg tccgtttga cattttaca 
      agtcggcaa cttaacggt gctacaacg tgcgtgttt 
      caacatcgg agtatagtg tttttttta tttgaaaac 
      tgtgttggg ggaatcacg cttcaaata attcaatta 
      cgagatgat ttcataatg cgagatgcc tcacacgat 
      cttacggcc ccatcatcc cgtcaatgg ctatgccca 
      tcattacac ccagaatgg acatgtcaa aaataatgg 
      gcatggtgg catgaacgg taccggaag accgtggat 
      gaacggaca cgctacgaa accggagga caagcagtc 
      gactttatc gaggtaatg atacaaata gacttaatg 
      gcggtgaaa aattccgca tgtttccga atagggttt 
      ccgattttt tttgacatt tttttgctt gaaacactg 
      gaagtttta aaatgattt taaaaaatt gtaagcttg 
      ttttaaata tttacatac ttacccatt ttttctaac 
      aatattaaa taaactaaa ctttgagtg
    • >TC005995 RC
      tttttcgaa agatgcagg aatataaca tggacaata 
      attttccca gatattgca ccactgaac ggcagcgcc 
      actcttcca actaccaag caatccgtc gagcatgtc 
      gcgagagag gctcccttt tcgaggacc cggattttc 
      cggcgggcc ctcgcgccc tctaccacc acaagaagc 
      cgtccctgc acccgatcg tgtggatga ggccccacg 
      aaatgtgcc agaggccgc gattcatca gcgacaaca 
      acaactccg gagagatgc gattcgctg tcgaagccg 
      gagaattgg gcgaccaat ggcttttgg cagctgtgg 
      cctctctgg ctctaacac ctcgattcc tggaccgga 
      tcgtgcctc cggatcaag gattcgaca actcgcata 
      actactgcg gagttttca ggtttcgat tctggcact 
      tcggcgaat ggaaggacg tgatagtgg acgacaagc 
      tccccactt tcaaggggc gcctcgtcc acttgcact 
      gctccaaca ccacggaat tctgggcag ctttgctcg 
      aaaaagcct acgccaaat tctacggct gttacgaga 
      atctggcgc aaggttcaa ccactcgcg ccctccaag 
      acctgaccg gtggcatcg tccaaagtt tcggcctca 
      ctcatcagg accgctttt tgacatttc aagtgttaa 
      acagcgctg ttcccagat ctagtctac tgattgcga 
      ccgtcactc aggaaaaag agaacaaaa gacagctga 
      ggctacgca atgggctga tcacccagc acgcgtaca 
      gtgtaacag ggttggccc gagtgagag ggccgcagg 
      gcgaaacac cgttggtaa gactgagaa acccatggg 
      cgcgggggg agtggacgg gggcgtgga gtgagcggt 
      cgtgggagt gggacgggc tgtctacca gggacaagg 
      agttgctga gtgtgcgcg tagcgaacg atggggagt 
      tctggatgt cgtttgagg acttttcca agcatttca 
      cgcagttgg atttggtcc atattgggc ctgatgatt 
      ggatgatgg aaagtgcct tacattcga agcaaccat 
      ggagggcgg ttctggcca ggaggcgct ggagggcgg 
      ggtataacg ctgggggcg ggcccagct atgttgaca 
      cgacgtcaa tgaatccac aatttcatg tccaaattc 
      cgaggactt caagcaata aatgtcacg ttgtggtgt 
      ccataacgc aaagttatg aaactagcg ttgctgata 
      gcaaaaaga agaagcctc tctatgcca tagggtttg 
      ccgtttatg aagtacctc attcgatgc aaaggctaa 
      ctcctcact tcgttgcgg acaagttaa aacctctag 
      atgtcacta accactcaa ttgccagag aagtcgtaa 
      ctttcttta ctttacctc ctggtgact acattgtag 
      taccacaaa ccaatatcc ccaacctgg acggcaaat 
      tccttttaa gagttttta cggacgaac agagcaaca 
      tttgggaag tcaacgaag ataacatga ttataagaa 
      acatttcgt cagaactga tggaagaaa caggacctg 
      ttacgcccg aaggcaagt caattttgt caaaactcc 
      tcacaaaat tccccccag aagttgatg ctagtcagt 
      tacaaaaaa ttctgaggg cccactgga agtcgtact 
      tgtccgaaa aaccgtcgt tggaactgt gcaaaagct 
      tgatcatgt tgcgtgatt acaacatta gtggccgca 
      ttaacttaa tggatattc ccgtgttga tgcatatgc 
      ttcaatttt ggagatgcg ctttccaaa aattcgaac 
      gaggtcacg gcaatggta aaacttcaa gttatcatc 
      ttcgagctc tgttatggg aggccgggt gtactgtta 
      gcaataaag ttctagaat gtttggtgc ttaggtttg 
      ccaaaaatc ggattctga caacagaga attacgtga 
      tggccttag tacgcttgc atttagcgc acgaacggt 
      accataaca ttgatacga agatgaaga gcaatcctt 
      tgtctttag aagaaatga tcttgatga ctatttatt 
      catgatagc tttccggtg atgtgtcta gaagccaaa 
      gaatctctc cctccagtc tgactgtaa ataaaatgt 
      gcaaatatt c
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