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Gene information
General information
TC033976
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005992 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4109912..4111266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033976
    iBeetle screen iB_04051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033976 RA
      MKTFLLLFG VVSLLGYST GIENYLGTE IQKCLNCIC 
HARTGCYSR FNCANYSID FDYWKTAGS PNVEEEDDE 
LEDNERFTK CMKNENCIL TTLDKYAEN IGHIDCNCD 
QKFDCRDRL AIHLLGDKC TNPKFMKRY LRRFNNCAR 
KLGVTTMFD EENYDGIKN YMGSDLQSC LNCLCHART 
GCFSRFNCA SYSISFDYW KTANSPTVD STDAPEAEA 
SFKKCMKNE NCILATLDQ YVDSMGHMD CNCDGQFDC 
KDRFAIHLH GANCTNPKF PDNYVARFN NCAKNLKVK 
AMVAEEGFE GCIPEVF
    CDS sequence
    • >TC033976 RA
      atgaaaaca tttctcctc ctctttggt gtggtctct 
ttgttgggt tattcaaca ggcattgaa aactactta 
ggcaccgaa atccagaaa tgcctcaac tgtatttgc 
catgccaga actggatgt tacagccgg tttaactgt 
gcaaattat tcaatcgat ttcgattac tggaaaaca 
gccggaagt ccaaacgtt gaggaagaa gacgatgaa 
ttggaagac aacgaacgg ttcaccaaa tgtatgaaa 
aacgaaaac tgtattctg accacactt gacaaatat 
gccgaaaat attggacac attgattgt aactgtgac 
caaaaattc gactgcaga gacagactt gctattcat 
ttgcttgga gacaaatgt actaacccg aaatttatg 
aaacgatac ctgagaagg tttaataat tgcgccaga 
aaattgggt gttacaaca atgtttgat gaggaaaat 
tacgatgga atcaaaaac tacatgggc tcagacctg 
caaagctgt ctcaactgt ctttgccat gcccgaaca 
ggttgtttc agtcgtttc aactgcgct agttattcc 
attagtttc gactactgg aaaacagcc aatagtcca 
actgttgac tccacagac gcgcctgaa gcggaagca 
agtttcaaa aaatgcatg aaaaatgag aactgcatt 
ctggcaacg cttgaccaa tatgtcgat tccatggga 
cacatggac tgcaactgc gacggacaa tttgactgt 
aaagacaga ttcgcaatt catttgcat ggagctaat 
tgtaccaat cctaaattt ccggataac tatgtagcg 
agatttaac aattgtgcc aaaaatttg aaagtgaaa 
gctatggtt gcggaggaa ggatttgaa ggctgcatt 
cctgaggtt ttttaa
    mRNA sequence
    • >TC033976 RA
      ttagttgta caaaatgaa aacatttct cctcctctt 
tggtgtggt ctctttgtt gggttattc aacaggcat 
tgaaaacta cttaggcac cgaaatcca gaaatgcct 
caactgtat ttgccatgc cagaactgg atgttacag 
ccggtttaa ctgtgcaaa ttattcaat cgatttcga 
ttactggaa aacagccgg aagtccaaa cgttgagga 
agaagacga tgaattgga agacaacga acggttcac 
caaatgtat gaaaaacga aaactgtat tctgaccac 
acttgacaa atatgccga aaatattgg acacattga 
ttgtaactg tgaccaaaa attcgactg cagagacag 
acttgctat tcatttgct tggagacaa atgtactaa 
cccgaaatt tatgaaacg atacctgag aaggtttaa 
taattgcgc cagaaaatt gggtgttac aacaatgtt 
tgatgagga aaattacga tggaatcaa aaactacat 
gggctcaga cctgcaaag ctgtctcaa ctgtctttg 
ccatgcccg aacaggttg tttcagtcg tttcaactg 
cgctagtta ttccattag tttcgacta ctggaaaac 
agccaatag tccaactgt tgactccac agacgcgcc 
tgaagcgga agcaagttt caaaaaatg catgaaaaa 
tgagaactg cattctggc aacgcttga ccaatatgt 
cgattccat gggacacat ggactgcaa ctgcgacgg 
acaatttga ctgtaaaga cagattcgc aattcattt 
gcatggagc taattgtac caatcctaa atttccgga 
taactatgt agcgagatt taacaattg tgccaaaaa 
tttgaaagt gaaagctat ggttgcgga ggaaggatt 
tgaaggctg cattcctga ggtttttta ataaataaa 
tgtattacc tattggttt tactcaata aaaaggaac 
gcatacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY