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Gene information
General information
TC005983 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3885506..3891779
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005983
    iBeetle screen iB_04050
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva anterior not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005983 RA
      MDFGGELSK QGLDTVRAL HQTVVALRQ ALEQSRTEI 
      LELKTKSFP IESVEQTLK TLALENHVL KTQLVDQAQ 
      KPVLEKKDD NRKLKKRKF EAKICVKST SQSLESLLV 
      LKSPTSKKL TVSKTVSFS NLSRPEMNN NETQEQNDV 
      EQEQEQDQD QEVDDIELI FTTDETKDS DFKEQLVSI 
      EAGDSSLLQ CPDLDQNLD ISDRELDDD VFNDNPEVD 
      FRRENSQLY NSKSDNSIN QEKSFKSCY SYQDSSFEN 
      KSLEKDESF DRFEERVRI VETDISKCG IQEVEYGGL 
      RRNTCPNPL QYRPLLHRE ALSKGSRKT RPILAHSNA 
      IRKDSGAQT DISALPGSS WLSESNLAN KTRGGENFP 
      TLPSKFPLP GSRLRLSEK TVEARRVLL SDIGFTSMV 
      PELSRSADH LCPGNLKPP VTYGQFLKT SDFHSPGFT 
      SQKFTWTTT TATPSEGSY SQSRYNSLY PLASPPIPS 
      RRCSAPVSP SRRAILRPT PSKVRFANG SLPELRGDW 
      TMTLDSGDS TDSLVEEAE NYLRRSIDC IVTTRDYGN 
      DGNKKSVPR RASAPEPSG DNVPPQGWQ PFLPRVPRD 
      LKPDHWVKV ITPEGRVRG GRVRYIGPV VSQNDQFVG 
      VQLGTPDGH SDGTFSTRR YFQCEPYHG IFVPFKKVI 
      MGWRP
    CDS sequence
    • >TC005983 RA
      atggatttt gggggggaa ttgtcgaaa caagggctg 
      gatactgta agggccctc caccagact gttgtcgct 
      ttgaggcaa gctttagag caaagtcgg actgaaata 
      ttagaactg aaaacgaaa agtttccca attgaatcg 
      gtcgaacaa accttgaaa acactagca ctcgagaat 
      cacgttttg aaaacgcag ctcgttgat caagctcaa 
      aaaccagta ttagagaaa aaggatgac aatcgtaag 
      ttgaaaaaa cgaaaattt gaggcaaaa atttgcgtg 
      aaatcgacg tcacagtca ctcgaaagt ctcctagtt 
      ttaaaatca ccaaccagc aaaaaacta actgtttcg 
      aaaacggtc agtttctcc aacctgtct cgaccagaa 
      atgaataat aacgaaacc caagaacag aatgacgta 
      gaacaggag caagagcaa gatcaggac caagaagtg 
      gacgatatt gaactgatc ttcaccaca gatgagacg 
      aaagattcg gatttcaaa gaacaacta gtgtctatc 
      gaagctggc gattcttcc ttactccaa tgcccggat 
      ttggaccag aatttggat atttccgat cgagaactg 
      gacgatgac gtgttcaac gataatccc gaagttgat 
      ttccgacgt gaaaattcg caactgtac aattccaag 
      tcggacaat tcgattaat caagagaaa agtttcaag 
      agttgttac tcgtatcaa gattccagt tttgagaat 
      aagtcttta gagaaagac gagagtttt gatcggttt 
      gaggaacgt gttaggatt gtggaaacg gatatttcg 
      aaatgtggg attcaggag gtggagtat ggggggctg 
      aggcgaaac acgtgtccc aatccgctg caatatcgg 
      cctcttttg caccgggag gccttgtcg aaaggctcc 
      cgcaagact cgcccgatc ttggcgcac tcgaacgca 
      atccgcaaa gactccggt gcccaaacg gacatttca 
      gctctgcct ggctcatcg tggctctcc gagagcaac 
      ttagcgaac aaaacaaga ggaggtgaa aatttccca 
      acgttacca tcaaaattc cccttgcct ggctctaga 
      ctacgccta tcggaaaaa acagtcgaa gctcgccga 
      gttttactt tccgatata ggttttacc agcatggtg 
      ccagagctg tctagatca gccgatcat ttatgccct 
      ggcaacctc aaaccacca gttacttat ggccagttt 
      ttgaaaact agcgatttt cattcgccg ggatttaca 
      tcccagaaa tttacttgg acgaccacc acggcaact 
      ccaagtgaa ggtagctac tctcagtcc cggtataat 
      agtctctat cccttagcc tcacctcct ataccttcc 
      cgtcggtgc tccgcccct gtttctccc tcacgtcga 
      gccatcctc agacctact ccatctaaa gttaggttt 
      gccaacggt tccttaccc gaattacga ggcgattgg 
      accatgact ttggatagt ggcgattcc acggatagt 
      ctagtagaa gaagcggaa aattactta agacggtcg 
      attgactgt attgtgacc acaagggat tatggaaat 
      gatggtaat aagaaaagt gtgcctcgc cgcgcctca 
      gcccccgaa ccctcagga gacaacgtc ccacctcaa 
      ggttggcaa ccgtttctc ccacgagtc ccacgtgac 
      ctcaaacca gaccattgg gtcaaggtt ataactcct 
      gaagggcgg gtcagagga ggccgcgtc cgctatatt 
      ggacctgta gtatcccaa aacgaccaa tttgtcggc 
      gtacagttg ggcacccct gatggtcat tctgatggt 
      acttttagt accagaagg tatttccaa tgcgagcct 
      taccatggt atttttgta ccgtttaag aaagtaatc 
      atgggttgg aggccataa
    mRNA sequence
    • >TC005983 RA
      tggccgcaa ataaattgg gttcaagtg cgcatatgt 
      ggataagtt ggcattcgg cccttgccc gcactgttt 
      catatttaa taaaagtgt catcggttg attggcaaa 
      gacgcagtt ttcgagcgg cgatttaag gggttttgg 
      tgcgcaaat cgccgaaaa agtcaaata ttaattgcc 
      cagttcgga gttagtgcc gtttgtgcg tcctcttct 
      aactcactt gtgtgtgca gtgggcgca tgtctgtgc 
      tcacttgtt aattcccgg ggttgtaca aaatccaag 
      gttcgagct tttgcggaa aatacagac tgtgtgaga 
      aaatggatg tgtttggtc gaaaatgga ttttggggg 
      ggaattgtc gaaacaagg gctggatac tgtaagggc 
      cctccacca gactgttgt cgctttgag gcaagcttt 
      agagcaaag tcggactga aatattaga actgaaaac 
      gaaaagttt cccaattga atcggtcga acaaacctt 
      gaaaacact agcactcga gaatcacgt tttgaaaac 
      gcagctcgt tgatcaagc tcaaaaacc agtattaga 
      gaaaaagga tgacaatcg taagttgaa aaaacgaaa 
      atttgaggc aaaaatttg cgtgaaatc gacgtcaca 
      gtcactcga aagtctcct agttttaaa atcaccaac 
      cagcaaaaa actaactgt ttcgaaaac ggtcagttt 
      ctccaacct gtctcgacc agaaatgaa taataacga 
      aacccaaga acagaatga cgtagaaca ggagcaaga 
      gcaagatca ggaccaaga agtggacga tattgaact 
      gatcttcac cacagatga gacgaaaga ttcggattt 
      caaagaaca actagtgtc tatcgaagc tggcgattc 
      ttccttact ccaatgccc ggatttgga ccagaattt 
      ggatatttc cgatcgaga actggacga tgacgtgtt 
      caacgataa tcccgaagt tgatttccg acgtgaaaa 
      ttcgcaact gtacaattc caagtcgga caattcgat 
      taatcaaga gaaaagttt caagagttg ttactcgta 
      tcaagattc cagttttga gaataagtc tttagagaa 
      agacgagag ttttgatcg gtttgagga acgtgttag 
      gattgtgga aacggatat ttcgaaatg tgggattca 
      ggaggtgga gtatggggg gctgaggcg aaacacgtg 
      tcccaatcc gctgcaata tcggcctct tttgcaccg 
      ggaggcctt gtcgaaagg ctcccgcaa gactcgccc 
      gatcttggc gcactcgaa cgcaatccg caaagactc 
      cggtgccca aacggacat ttcagctct gcctggctc 
      atcgtggct ctccgagag caacttagc gaacaaaac 
      aagaggagg tgaaaattt cccaacgtt accatcaaa 
      attcccctt gcctggctc tagactacg cctatcgga 
      aaaaacagt cgaagctcg ccgagtttt actttccga 
      tataggttt taccagcat ggtgccaga gctgtctag 
      atcagccga tcatttatg ccctggcaa cctcaaacc 
      accagttac ttatggcca gtttttgaa aactagcga 
      ttttcattc gccgggatt tacatccca gaaatttac 
      ttggacgac caccacggc aactccaag tgaaggtag 
      ctactctca gtcccggta taatagtct ctatccctt 
      agcctcacc tcctatacc ttcccgtcg gtgctccgc 
      ccctgtttc tccctcacg tcgagccat cctcagacc 
      tactccatc taaagttag gtttgccaa cggttcctt 
      acccgaatt acgaggcga ttggaccat gactttgga 
      tagtggcga ttccacgga tagtctagt agaagaagc 
      ggaaaatta cttaagacg gtcgattga ctgtattgt 
      gaccacaag ggattatgg aaatgatgg taataagaa 
      aagtgtgcc tcgccgcgc ctcagcccc cgaaccctc 
      aggagacaa cgtcccacc tcaaggttg gcaaccgtt 
      tctcccacg agtcccacg tgacctcaa accagacca 
      ttgggtcaa ggttataac tcctgaagg gcgggtcag 
      aggaggccg cgtccgcta tattggacc tgtagtatc 
      ccaaaacga ccaatttgt cggcgtaca gttgggcac 
      ccctgatgg tcattctga tggtacttt tagtaccag 
      aaggtattt ccaatgcga gccttacca tggtatttt 
      tgtaccgtt taagaaagt aatcatggg ttggaggcc 
      ataatagcg ggatcattt gtttcttca tgtgccata 
      tgttattgt ttaagcaat ataatattg attgaaatt 
      aggccaatt tgtgttcaa ttgggttta ttattggag 
      actccaatc gcttcctat gaccacaga cttcgtgat 
      cacagacgg cgccttccc atcacatta aaaatgttt 
      cgcctcggt ttatagaaa ataataatt cttccgtgg 
      gaggaaaaa acctactct gcactttta aatcaggaa 
      gcaaaatgt tatttcctg taaaatgag gcgaaaaaa 
      ttatatata tgtcacggg gacaaagta aattttctt 
      tcttgtaca attacagga aattttaaa cgttgttct 
      cttcatata cagactgaa ctaaaagta acgcacaat 
      a