Status
Please wait ...
Gene information
General information
TC005941 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5144825..5147266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005941
      iBeetle screen iB_04041

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva cuticle partially darker - Penetrance (percentage of animals/eggs on slide): less than 30%
      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head ventral bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      head irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      thorax irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
      Days post injection



      Adult morphology
      elytra dorsal orientation not closed - Number of animals showing the phenotype: 2


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005941 RA
        MASRVASKT GSKPSMLRT PRSQTSAAK AKPPEQEKE 
        SKFELPALS VPFKPKTTT EAELKKMKQ RMMRRHAPP 
        GECIKGIKT VLQSLKKKE VTVQEFIGL VAPYTADSG 
        AVLRSVMND DLNSFRKLA RKVYETITQ DNSDVLLNQ 
        QLQLAEYYR EKKALLHDK MMRQIEEIY MMAPNFDFD 
        RYAAEKEKY LKELFPIPD ELQVEPEEI EMMKRQAAY 
        HRLQWLRSE GERLNEENN RLKTRLQQL KQMHKEEQR 
        KMMEAELEA EAKKEALEA EQAAKVETL EQLNESLEK 
        TIISKETTI REKTDQSAL SAPHPSKVQ KKKTKAAVR 
        KKRPAWNEV AAYEMEVEA AEAAQVTEA AAEMEREQS 
        FHYTPTRET RPQVVQYGR RGAARPKDT WGGFY
      CDS sequence
      • >TC005941 RA
        atggcgagt cgagtcgca tccaaaacc ggttcaaaa 
        ccgtcaatg ttgcgcacg ccaagaagc caaacatcg 
        gcagcgaag gcaaagccc cccgaacaa gaaaaagaa 
        tccaaattt gaattaccc gcgcttagt gttcccttc 
        aagccgaag accacgacc gaagccgaa ctgaagaag 
        atgaagcag cggatgatg aggcggcat gcgccccct 
        ggggagtgc atcaaaggg ataaagacc gttcttcag 
        agtttgaaa aagaaggaa gtgacggtt caggagttt 
        atagggctg gtggcgccc tacactgcc gactctggc 
        gccgtcttg cggagcgtt atgaacgac gatttgaat 
        tcctttcgg aaattggcg cgaaaagtc tacgaaact 
        ataacgcag gataatagc gatgttctc ctcaaccaa 
        cagttgcaa ctggccgag tattacagg gagaagaag 
        gctttgctt catgataag atgatgcgc caaatcgaa 
        gaaatttac atgatggcg ccaaatttt gattttgac 
        agatacgcc gccgagaag gagaaatac ctcaaggaa 
        ctcttccca ataccggat gagttgcaa gtggagcct 
        gaagaaatc gagatgatg aagcgccag gcggcctac 
        caccgcctc cagtggctg cgttcggaa ggtgaacgc 
        ctaaacgag gagaacaat cggctgaaa acccggctc 
        cagcaactc aagcaaatg cacaaggag gagcaacgt 
        aagatgatg gaagccgag ctggaggcc gaagccaaa 
        aaggaagcc ctcgaagct gagcaagcg gccaaagtt 
        gaaacactc gaacaactg aacgaatcg cttgaaaag 
        acaatcatt tcgaaagaa acaaccatc cgggaaaag 
        acagatcag agcgcattg agtgccccc cacccctcc 
        aaagtacaa aagaagaag accaaagcg gcggtgcgt 
        aaaaaacgc cccgcctgg aacgaggta gcggcttat 
        gagatggag gtggaggca gcggaggcg gcgcaagta 
        actgaagcg gcggctgaa atggagagg gaacaatcg 
        tttcattat acgccgacg cgagaaaca cgcccacag 
        gtggtgcag tatggtagg aggggcgct gcgaggccc 
        aaagataca tggggcggg ttttattaa
      mRNA sequence
      • >TC005941 RA
        tctatcacc tgtaagggc tacgcacac atttaataa 
        atcaagtcg ctgcgcact caaacgtca ttaaaaatg 
        ttggcaaag tccaaaaac ttctaaatt ttgcattcc 
        ggccccagt tttgaaaat gctagtact gataatccc 
        tctcgaggt actgatttt agtgacttg gtttaacat 
        cctaatcat tacgtgttc gttgtgttt aatttaaaa 
        tttccggca attttcctc aaaattttc cgtaatggc 
        gagtcgagt cgcatccaa aaccggttc aaaaccgtc 
        aatgttgcg cacgccaag aagccaaac atcggcagc 
        gaaggcaaa gccccccga acaagaaaa agaatccaa 
        atttgaatt acccgcgct tagtgttcc cttcaagcc 
        gaagaccac gaccgaagc cgaactgaa gaagatgaa 
        gcagcggat gatgaggcg gcatgcgcc ccctgggga 
        gtgcatcaa agggataaa gaccgttct tcagagttt 
        gaaaaagaa ggaagtgac ggttcagga gtttatagg 
        gctggtggc gccctacac tgccgactc tggcgccgt 
        cttgcggag cgttatgaa cgacgattt gaattcctt 
        tcggaaatt ggcgcgaaa agtctacga aactataac 
        gcaggataa tagcgatgt tctcctcaa ccaacagtt 
        gcaactggc cgagtatta cagggagaa gaaggcttt 
        gcttcatga taagatgat gcgccaaat cgaagaaat 
        ttacatgat ggcgccaaa ttttgattt tgacagata 
        cgccgccga gaaggagaa atacctcaa ggaactctt 
        cccaatacc ggatgagtt gcaagtgga gcctgaaga 
        aatcgagat gatgaagcg ccaggcggc ctaccaccg 
        cctccagtg gctgcgttc ggaaggtga acgcctaaa 
        cgaggagaa caatcggct gaaaacccg gctccagca 
        actcaagca aatgcacaa ggaggagca acgtaagat 
        gatggaagc cgagctgga ggccgaagc caaaaagga 
        agccctcga agctgagca agcggccaa agttgaaac 
        actcgaaca actgaacga atcgcttga aaagacaat 
        catttcgaa agaaacaac catccggga aaagacaga 
        tcagagcgc attgagtgc cccccaccc ctccaaagt 
        acaaaagaa gaagaccaa agcggcggt gcgtaaaaa 
        acgccccgc ctggaacga ggtagcggc ttatgagat 
        ggaggtgga ggcagcgga ggcggcgca agtaactga 
        agcggcggc tgaaatgga gagggaaca atcgtttca 
        ttatacgcc gacgcgaga aacacgccc acaggtggt 
        gcagtatgg taggagggg cgctgcgag gcccaaaga 
        tacatgggg cgggtttta ttaaaacaa ggcggcgaa 
        taaattatt tcatgttga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY