| General information | |||
| TC005933 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 5362674..5373036 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005933 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04040 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
remarks: muscle pattern potentially obscured by segmentation defect,
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC005933 RA
MSTLPILQP RLILGLRTD VKGNAQFIT DEEIIYPVG AVLTIHNLN QKRQKYIKL ADKGKNVTE VVVSPNKKL VAVAETGEK PFITLWCPL TYKKKRQIN LPTDKEISA EKFTAVAFT FDSKYIVCV SGEPDWTLY YFRCDKGKL ESSARANNI SNTGYVRQI ACNPNDANL LALVGDGLI RILACTDYT WRQYGYSKG DQWNFSSAV WLTQDRLLV GTLDGRLLV IESGELKFV FSAADLAVM DLKPKDELQ QSSQTSLKV LSDDEIDPN EDYSIRSLI QFPRGFAFG YLTGTVHLY EMETPHKFI KRNIFKIPD HTITREYEE ETEQKMTTV NCVSINPSQ GKLMVTCRE SQIYNATLW MQDSAITTA VESMFTEYG PALHNGPIG SMAVCNWKP ILLTAGRND RTLKIWNYE TEELELMQQ FQDDLYGVA LHPSGLYAI VGFSDKLRF LTVMIEEFE MTREFNIRV CKMCVFSRL GYLFAAANG NVIQVYSSI TFEMLYNLK GHNGRIFGM SWTADDRKM ASCGSEGAV YEWVVQESK RLSETIMKQ CEFKGVAIT TDGRSIFVV ANDGHIREL MNSNVHRDV LVTTAGLDS MVMSKIDQM LFVAGNGGT VYNIKLPLL EKAEYHEYS IHADVVTQM LVSFDDKYL FTGSDEGTI CFWKLLNTE GKAIKMDKD FQPSKDILI SRQILEERI AQTKNLQMR LKELESEYA YQLRQNDVV NSLRMKEIH SGYCQAIEE LKEKNEQLE NDHVQEIHN INLDIKQMK LDHEEFVRE LEANYNEKL IFEYEKYLR LEEKMEKMR RYYEKELDD LRKAKQDSE EIITNGFLE KLEEKQMQF EELVEQTAR KTKAHEVIK QQIEDDADR EIYELKAAH AKELKEEQE LNVKIRGET AIVKKKLIS SQKEIDELK SKVHTLDHD HAKLKKTII NLEKDIVDL KKEISERDA TIQDKEKRI FDLKRKNQE LEKFKFILD FKIKELKSQ IEPREQMIQ EQTEQINAM VKELENLQR VILSLDLQL HELREKLYA SDNEVKKQI AKNAAMNRA LKKIKTDIF LASGQIQNV PLLIKTVKE MYHKFNIDE DFELTQAED TEVKSEFLR QREFLERTV TSLQMQVNK STSTLSSDK VKLTEENAV LLVETNMLR KQLKDEQRR KDKLQSILG VNQKYLPPK VAMERVQNA TATREEIKK EYRKTIEYN EKSMQALRE ENDRLLNKI LVEASNTTE MSPTAGELS IQEDNKND
- >TC005933 RA
atgtcgact ttaccaatc cttcaaccc cgtcttatt ttaggctta cgaaccgat gttaaaggc aatgcccaa ttcatcaca gacgaagaa ataatttac cccgtgggg gcggtttta accatccac aacctcaac caaaaacgc caaaaatac atcaaactt gcagacaag ggtaaaaat gtgactgaa gtggtggtc agtcccaac aaaaaacta gtcgccgtg gctgaaaca ggagaaaaa cccttcata accctgtgg tgcccctta acttacaaa aagaaacgc caaattaac cttccaact gataaggaa atttcagct gagaaattc acagcagtt gcgtttact tttgactcg aaatacatt gtttgtgtg agtggggaa cctgactgg accttgtac tacttcagg tgcgataag ggcaaactt gagagtagt gctagggct aataatatt agcaatacg gggtatgtg aggcagatc gcttgcaat cccaacgat gctaatctt ttagccctg gttggtgat gggctaatc cgaattttg gcttgtact gactacacc tggaggcag tatgggtac agcaaaggc gatcagtgg aactttagc tcagccgtt tggttaaca caggaccgg cttttggtg ggtactctc gatgggcgc cttttggtt attgaaagt ggcgagttg aaatttgtt ttttcggcc gctgattta gcagtgatg gacttgaaa ccaaaagac gagttgcaa caatcgtct caaaccagc ctcaaagtt ttgagtgac gacgaaatt gacccgaat gaggattac agcatccgc tccctaatc cagtttccg cgcggtttt gcctttggt tacctaacc ggaaccgtg cacttgtac gaaatggaa acccctcac aaattcatc aaacgcaac atcttcaaa atccccgac cacacaata acccgcgag tacgaggaa gaaaccgag cagaaaatg accacagtc aactgcgtc tccatcaac ccatcgcag ggcaagctc atggtcact tgcagggag agccagatc tacaacgcg actctctgg atgcaagac tccgcaatc accaccgca gtcgagtca atgttcacc gaatatggg ccagccctc cataacgga cccatcggg agcatggcg gtgtgcaac tggaagccc atcctgctc acggctggt cgcaacgac cgtactttg aaaatctgg aactacgag acggaggaa ctggagctg atgcagcag ttccaggac gatttgtac ggagttgcc ttgcacccg agtggcctt tatgccatt gttggcttc tcggacaag ttgcggttt ctgactgtg atgatcgaa gagtttgaa atgacgcga gagtttaac atccgcgtt tgcaagatg tgtgtcttc agcaggctg gggtacttg tttgctgct gcgaacggg aacgttatt caagtttac tcgagtatt actttcgaa atgttgtat aacttgaag gggcacaat gggcgcatt tttggcatg tcttggacg gccgatgac cggaagatg gcgtcgtgt gggagcgag ggggctgtg tatgagtgg gtggtgcag gagtcgaaa cggttgtcg gaaactata atgaagcag tgcgagttt aaaggagtt gcgattacg acggatggg aggtcgatt tttgtcgtg gcaaatgac gggcatatc agggaattg atgaattcg aacgtccat agagatgtt ttggtcaca acggccggt ttggactcg atggttatg tcgaaaatc gaccaaatg ctgttcgtg gctggaaat ggggggact gtttacaat attaagttg ccactgttg gaaaaagcc gagtatcac gagtatagc attcatgcc gatgttgtc acacaaatg ttagtgtct ttcgacgac aagtacctt ttcaccggt tcagatgag ggcacaatt tgcttttgg aagttgtta aataccgaa gggaaagct atcaaaatg gataaggat tttcaaccg tcgaaggat atcctcatt tctcgccaa atccttgag gaacgaatt gcacaaact aagaatctt caaatgagg ttgaaggaa ctagagtcg gaatatgcc tatcagtta aggcaaaat gacgtcgtc aatagtttg aggatgaag gagatacat tctggttac tgtcaagca atcgaggaa ctgaaggaa aaaaacgaa caactcgaa aatgaccac gtgcaagaa atccacaac atcaatcta gacatcaag caaatgaaa ttggaccat gaggagttc gtgcgcgaa ttggaggcc aactacaac gaaaaatta attttcgaa tacgagaaa tacctccga ctggaggaa aaaatggaa aagatgaga cgctactac gagaaagag ttagacgac ttgagaaaa gcgaaacaa gactcggag gagataata acgaatggg ttcctcgaa aaattggag gagaaacag atgcagttt gaggagttg gttgaacaa accgctcgc aaaaccaaa gcccacgaa gtgatcaaa caacaaatt gaagatgac gccgatcga gagatttac gaattgaag gcggcccat gccaaagaa ctcaaagaa gagcaagag ttgaacgtg aagattcga ggcgaaacg gcgatcgtg aagaaaaaa ctgatttca tcgcagaag gaaatcgac gaattgaaa agcaaagtg cacactttg gaccacgac catgccaag ttgaagaaa acaatcatc aatcttgag aaagatatc gtcgatttg aagaaggag atctcggaa cgcgacgcc acgattcaa gacaaagaa aaacgcatc tttgatttg aagcggaag aaccaagag ttggagaag tttaagttc atccttgac ttcaagatc aaagaactg aaatcgcaa atcgagccc agggagcaa atgattcaa gaacaaacc gagcaaatc aatgccatg gttaaagaa ctcgagaat ttgcaacga gttattttg agtcttgat ttacaactg cacgaattg agggaaaaa ctttacgct tcggataac gaagtgaag aagcaaatt gcgaagaac gcagcgatg aaccgagca ttgaagaag atcaagact gatattttc ctagcgagt ggtcagatt cagaatgtc ccacttctg attaaaact gtgaaggaa atgtaccac aagttcaac atcgacgaa gatttcgaa ctgacccaa gctgaagac acggaagtg aagagcgag tttctgaga cagagggag tttttggag aggacggtg acaagtctt cagatgcaa gttaataag agtacttcg actttgagt tcggataag gtgaagttg actgaggag aacgccgtt ttgttggtc gagaccaac atgttgcgg aagcaactc aaagacgag cagaggagg aaggataag ttgcagtcg attctcggg gtgaaccag aagtatttg ccgccgaaa gtcgcaatg gaacgggtg caaaatgcg acagcaaca agggaggaa attaagaag gagtaccgg aaaacgatc gagtacaat gagaagagt atgcaagct ttgagggaa gagaacgac agactcttg aataaaatt ctagtcgag gcgagtaac acgacggaa atgtcgcca acagctggt gaattgtca atacaggag gataataaa aatgattaa
- >TC005933 RA
aaatcacca aacgtcaat aatgtcgac tttaccaat ccttcaacc ccgtcttat tttaggctt acgaaccga tgttaaagg caatgccca attcatcac agacgaaga aataattta ccccgtggg ggcggtttt aaccatcca caacctcaa ccaaaaacg ccaaaaata catcaaact tgcagacaa gggtaaaaa tgtgactga agtggtggt cagtcccaa caaaaaact agtcgccgt ggctgaaac aggagaaaa acccttcat aaccctgtg gtgcccctt aacttacaa aaagaaacg ccaaattaa ccttccaac tgataagga aatttcagc tgagaaatt cacagcagt tgcgtttac ttttgactc gaaatacat tgtttgtgt gagtgggga acctgactg gaccttgta ctacttcag gtgcgataa gggcaaact tgagagtag tgctagggc taataatat tagcaatac ggggtatgt gaggcagat cgcttgcaa tcccaacga tgctaatct tttagccct ggttggtga tgggctaat ccgaatttt ggcttgtac tgactacac ctggaggca gtatgggta cagcaaagg cgatcagtg gaactttag ctcagccgt ttggttaac acaggaccg gcttttggt gggtactct cgatgggcg ccttttggt tattgaaag tggcgagtt gaaatttgt tttttcggc cgctgattt agcagtgat ggacttgaa accaaaaga cgagttgca acaatcgtc tcaaaccag cctcaaagt tttgagtga cgacgaaat tgacccgaa tgaggatta cagcatccg ctccctaat ccagtttcc gcgcggttt tgcctttgg ttacctaac cggaaccgt gcacttgta cgaaatgga aacccctca caaattcat caaacgcaa catcttcaa aatccccga ccacacaat aacccgcga gtacgagga agaaaccga gcagaaaat gaccacagt caactgcgt ctccatcaa cccatcgca gggcaagct catggtcac ttgcaggga gagccagat ctacaacgc gactctctg gatgcaaga ctccgcaat caccaccgc agtcgagtc aatgttcac cgaatatgg gccagccct ccataacgg acccatcgg gagcatggc ggtgtgcaa ctggaagcc catcctgct cacggctgg tcgcaacga ccgtacttt gaaaatctg gaactacga gacggagga actggagct gatgcagca gttccagga cgatttgta cggagttgc cttgcaccc gagtggcct ttatgccat tgttggctt ctcggacaa gttgcggtt tctgactgt gatgatcga agagtttga aatgacgcg agagtttaa catccgcgt ttgcaagat gtgtgtctt cagcaggct ggggtactt gtttgctgc tgcgaacgg gaacgttat tcaagttta ctcgagtat tactttcga aatgttgta taacttgaa ggggcacaa tgggcgcat ttttggcat gtcttggac ggccgatga ccggaagat ggcgtcgtg tgggagcga gggggctgt gtatgagtg ggtggtgca ggagtcgaa acggttgtc ggaaactat aatgaagca gtgcgagtt taaaggagt tgcgattac gacggatgg gaggtcgat ttttgtcgt ggcaaatga cgggcatat cagggaatt gatgaattc gaacgtcca tagagatgt tttggtcac aacggccgg tttggactc gatggttat gtcgaaaat cgaccaaat gctgttcgt ggctggaaa tggggggac tgtttacaa tattaagtt gccactgtt ggaaaaagc cgagtatca cgagtatag cattcatgc cgatgttgt cacacaaat gttagtgtc tttcgacga caagtacct tttcaccgg ttcagatga gggcacaat ttgcttttg gaagttgtt aaataccga agggaaagc tatcaaaat ggataagga ttttcaacc gtcgaagga tatcctcat ttctcgcca aatccttga ggaacgaat tgcacaaac taagaatct tcaaatgag gttgaagga actagagtc ggaatatgc ctatcagtt aaggcaaaa tgacgtcgt caatagttt gaggatgaa ggagataca ttctggtta ctgtcaagc aatcgagga actgaagga aaaaaacga acaactcga aaatgacca cgtgcaaga aatccacaa catcaatct agacatcaa gcaaatgaa attggacca tgaggagtt cgtgcgcga attggaggc caactacaa cgaaaaatt aattttcga atacgagaa atacctccg actggagga aaaaatgga aaagatgag acgctacta cgagaaaga gttagacga cttgagaaa agcgaaaca agactcgga ggagataat aacgaatgg gttcctcga aaaattgga ggagaaaca gatgcagtt tgaggagtt ggttgaaca aaccgctcg caaaaccaa agcccacga agtgatcaa acaacaaat tgaagatga cgccgatcg agagattta cgaattgaa ggcggccca tgccaaaga actcaaaga agagcaaga gttgaacgt gaagattcg aggcgaaac ggcgatcgt gaagaaaaa actgatttc atcgcagaa ggaaatcga cgaattgaa aagcaaagt gcacacttt ggaccacga ccatgccaa gttgaagaa aacaatcat caatcttga gaaagatat cgtcgattt gaagaagga gatctcgga acgcgacgc cacgattca agacaaaga aaaacgcat ctttgattt gaagcggaa gaaccaaga gttggagaa gtttaagtt catccttga cttcaagat caaagaact gaaatcgca aatcgagcc cagggagca aatgattca agaacaaac cgagcaaat caatgccat ggttaaaga actcgagaa tttgcaacg agttatttt gagtcttga tttacaact gcacgaatt gagggaaaa actttacgc ttcggataa cgaagtgaa gaagcaaat tgcgaagaa cgcagcgat gaaccgagc attgaagaa gatcaagac tgatatttt cctagcgag tggtcagat tcagaatgt cccacttct gattaaaac tgtgaagga aatgtacca caagttcaa catcgacga agatttcga actgaccca agctgaaga cacggaagt gaagagcga gtttctgag acagaggga gtttttgga gaggacggt gacaagtct tcagatgca agttaataa gagtacttc gactttgag ttcggataa ggtgaagtt gactgagga gaacgccgt tttgttggt cgagaccaa catgttgcg gaagcaact caaagacga gcagaggag gaaggataa gttgcagtc gattctcgg ggtgaacca gaagtattt gccgccgaa agtcgcaat ggaacgggt gcaaaatgc gacagcaac aagggagga aattaagaa ggagtaccg gaaaacgat cgagtacaa tgagaagag tatgcaagc tttgaggga agagaacga cagactctt gaataaaat tctagtcga ggcgagtaa cacgacgga aatgtcgcc aacagctgg tgaattgtc aatacagga ggataataa aaatgatta ataaaacaa atcaacgtt tattaattt aacaataaa gtaacattt tttaa
