Status
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Gene information
General information
TC005933 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5362674..5373036
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005933
    iBeetle screen iB_04040

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005933 RA
      MSTLPILQP RLILGLRTD VKGNAQFIT DEEIIYPVG 
      AVLTIHNLN QKRQKYIKL ADKGKNVTE VVVSPNKKL 
      VAVAETGEK PFITLWCPL TYKKKRQIN LPTDKEISA 
      EKFTAVAFT FDSKYIVCV SGEPDWTLY YFRCDKGKL 
      ESSARANNI SNTGYVRQI ACNPNDANL LALVGDGLI 
      RILACTDYT WRQYGYSKG DQWNFSSAV WLTQDRLLV 
      GTLDGRLLV IESGELKFV FSAADLAVM DLKPKDELQ 
      QSSQTSLKV LSDDEIDPN EDYSIRSLI QFPRGFAFG 
      YLTGTVHLY EMETPHKFI KRNIFKIPD HTITREYEE 
      ETEQKMTTV NCVSINPSQ GKLMVTCRE SQIYNATLW 
      MQDSAITTA VESMFTEYG PALHNGPIG SMAVCNWKP 
      ILLTAGRND RTLKIWNYE TEELELMQQ FQDDLYGVA 
      LHPSGLYAI VGFSDKLRF LTVMIEEFE MTREFNIRV 
      CKMCVFSRL GYLFAAANG NVIQVYSSI TFEMLYNLK 
      GHNGRIFGM SWTADDRKM ASCGSEGAV YEWVVQESK 
      RLSETIMKQ CEFKGVAIT TDGRSIFVV ANDGHIREL 
      MNSNVHRDV LVTTAGLDS MVMSKIDQM LFVAGNGGT 
      VYNIKLPLL EKAEYHEYS IHADVVTQM LVSFDDKYL 
      FTGSDEGTI CFWKLLNTE GKAIKMDKD FQPSKDILI 
      SRQILEERI AQTKNLQMR LKELESEYA YQLRQNDVV 
      NSLRMKEIH SGYCQAIEE LKEKNEQLE NDHVQEIHN 
      INLDIKQMK LDHEEFVRE LEANYNEKL IFEYEKYLR 
      LEEKMEKMR RYYEKELDD LRKAKQDSE EIITNGFLE 
      KLEEKQMQF EELVEQTAR KTKAHEVIK QQIEDDADR 
      EIYELKAAH AKELKEEQE LNVKIRGET AIVKKKLIS 
      SQKEIDELK SKVHTLDHD HAKLKKTII NLEKDIVDL 
      KKEISERDA TIQDKEKRI FDLKRKNQE LEKFKFILD 
      FKIKELKSQ IEPREQMIQ EQTEQINAM VKELENLQR 
      VILSLDLQL HELREKLYA SDNEVKKQI AKNAAMNRA 
      LKKIKTDIF LASGQIQNV PLLIKTVKE MYHKFNIDE 
      DFELTQAED TEVKSEFLR QREFLERTV TSLQMQVNK 
      STSTLSSDK VKLTEENAV LLVETNMLR KQLKDEQRR 
      KDKLQSILG VNQKYLPPK VAMERVQNA TATREEIKK 
      EYRKTIEYN EKSMQALRE ENDRLLNKI LVEASNTTE 
      MSPTAGELS IQEDNKND
    CDS sequence
    • >TC005933 RA
      atgtcgact ttaccaatc cttcaaccc cgtcttatt 
      ttaggctta cgaaccgat gttaaaggc aatgcccaa 
      ttcatcaca gacgaagaa ataatttac cccgtgggg 
      gcggtttta accatccac aacctcaac caaaaacgc 
      caaaaatac atcaaactt gcagacaag ggtaaaaat 
      gtgactgaa gtggtggtc agtcccaac aaaaaacta 
      gtcgccgtg gctgaaaca ggagaaaaa cccttcata 
      accctgtgg tgcccctta acttacaaa aagaaacgc 
      caaattaac cttccaact gataaggaa atttcagct 
      gagaaattc acagcagtt gcgtttact tttgactcg 
      aaatacatt gtttgtgtg agtggggaa cctgactgg 
      accttgtac tacttcagg tgcgataag ggcaaactt 
      gagagtagt gctagggct aataatatt agcaatacg 
      gggtatgtg aggcagatc gcttgcaat cccaacgat 
      gctaatctt ttagccctg gttggtgat gggctaatc 
      cgaattttg gcttgtact gactacacc tggaggcag 
      tatgggtac agcaaaggc gatcagtgg aactttagc 
      tcagccgtt tggttaaca caggaccgg cttttggtg 
      ggtactctc gatgggcgc cttttggtt attgaaagt 
      ggcgagttg aaatttgtt ttttcggcc gctgattta 
      gcagtgatg gacttgaaa ccaaaagac gagttgcaa 
      caatcgtct caaaccagc ctcaaagtt ttgagtgac 
      gacgaaatt gacccgaat gaggattac agcatccgc 
      tccctaatc cagtttccg cgcggtttt gcctttggt 
      tacctaacc ggaaccgtg cacttgtac gaaatggaa 
      acccctcac aaattcatc aaacgcaac atcttcaaa 
      atccccgac cacacaata acccgcgag tacgaggaa 
      gaaaccgag cagaaaatg accacagtc aactgcgtc 
      tccatcaac ccatcgcag ggcaagctc atggtcact 
      tgcagggag agccagatc tacaacgcg actctctgg 
      atgcaagac tccgcaatc accaccgca gtcgagtca 
      atgttcacc gaatatggg ccagccctc cataacgga 
      cccatcggg agcatggcg gtgtgcaac tggaagccc 
      atcctgctc acggctggt cgcaacgac cgtactttg 
      aaaatctgg aactacgag acggaggaa ctggagctg 
      atgcagcag ttccaggac gatttgtac ggagttgcc 
      ttgcacccg agtggcctt tatgccatt gttggcttc 
      tcggacaag ttgcggttt ctgactgtg atgatcgaa 
      gagtttgaa atgacgcga gagtttaac atccgcgtt 
      tgcaagatg tgtgtcttc agcaggctg gggtacttg 
      tttgctgct gcgaacggg aacgttatt caagtttac 
      tcgagtatt actttcgaa atgttgtat aacttgaag 
      gggcacaat gggcgcatt tttggcatg tcttggacg 
      gccgatgac cggaagatg gcgtcgtgt gggagcgag 
      ggggctgtg tatgagtgg gtggtgcag gagtcgaaa 
      cggttgtcg gaaactata atgaagcag tgcgagttt 
      aaaggagtt gcgattacg acggatggg aggtcgatt 
      tttgtcgtg gcaaatgac gggcatatc agggaattg 
      atgaattcg aacgtccat agagatgtt ttggtcaca 
      acggccggt ttggactcg atggttatg tcgaaaatc 
      gaccaaatg ctgttcgtg gctggaaat ggggggact 
      gtttacaat attaagttg ccactgttg gaaaaagcc 
      gagtatcac gagtatagc attcatgcc gatgttgtc 
      acacaaatg ttagtgtct ttcgacgac aagtacctt 
      ttcaccggt tcagatgag ggcacaatt tgcttttgg 
      aagttgtta aataccgaa gggaaagct atcaaaatg 
      gataaggat tttcaaccg tcgaaggat atcctcatt 
      tctcgccaa atccttgag gaacgaatt gcacaaact 
      aagaatctt caaatgagg ttgaaggaa ctagagtcg 
      gaatatgcc tatcagtta aggcaaaat gacgtcgtc 
      aatagtttg aggatgaag gagatacat tctggttac 
      tgtcaagca atcgaggaa ctgaaggaa aaaaacgaa 
      caactcgaa aatgaccac gtgcaagaa atccacaac 
      atcaatcta gacatcaag caaatgaaa ttggaccat 
      gaggagttc gtgcgcgaa ttggaggcc aactacaac 
      gaaaaatta attttcgaa tacgagaaa tacctccga 
      ctggaggaa aaaatggaa aagatgaga cgctactac 
      gagaaagag ttagacgac ttgagaaaa gcgaaacaa 
      gactcggag gagataata acgaatggg ttcctcgaa 
      aaattggag gagaaacag atgcagttt gaggagttg 
      gttgaacaa accgctcgc aaaaccaaa gcccacgaa 
      gtgatcaaa caacaaatt gaagatgac gccgatcga 
      gagatttac gaattgaag gcggcccat gccaaagaa 
      ctcaaagaa gagcaagag ttgaacgtg aagattcga 
      ggcgaaacg gcgatcgtg aagaaaaaa ctgatttca 
      tcgcagaag gaaatcgac gaattgaaa agcaaagtg 
      cacactttg gaccacgac catgccaag ttgaagaaa 
      acaatcatc aatcttgag aaagatatc gtcgatttg 
      aagaaggag atctcggaa cgcgacgcc acgattcaa 
      gacaaagaa aaacgcatc tttgatttg aagcggaag 
      aaccaagag ttggagaag tttaagttc atccttgac 
      ttcaagatc aaagaactg aaatcgcaa atcgagccc 
      agggagcaa atgattcaa gaacaaacc gagcaaatc 
      aatgccatg gttaaagaa ctcgagaat ttgcaacga 
      gttattttg agtcttgat ttacaactg cacgaattg 
      agggaaaaa ctttacgct tcggataac gaagtgaag 
      aagcaaatt gcgaagaac gcagcgatg aaccgagca 
      ttgaagaag atcaagact gatattttc ctagcgagt 
      ggtcagatt cagaatgtc ccacttctg attaaaact 
      gtgaaggaa atgtaccac aagttcaac atcgacgaa 
      gatttcgaa ctgacccaa gctgaagac acggaagtg 
      aagagcgag tttctgaga cagagggag tttttggag 
      aggacggtg acaagtctt cagatgcaa gttaataag 
      agtacttcg actttgagt tcggataag gtgaagttg 
      actgaggag aacgccgtt ttgttggtc gagaccaac 
      atgttgcgg aagcaactc aaagacgag cagaggagg 
      aaggataag ttgcagtcg attctcggg gtgaaccag 
      aagtatttg ccgccgaaa gtcgcaatg gaacgggtg 
      caaaatgcg acagcaaca agggaggaa attaagaag 
      gagtaccgg aaaacgatc gagtacaat gagaagagt 
      atgcaagct ttgagggaa gagaacgac agactcttg 
      aataaaatt ctagtcgag gcgagtaac acgacggaa 
      atgtcgcca acagctggt gaattgtca atacaggag 
      gataataaa aatgattaa
    mRNA sequence
    • >TC005933 RA
      aaatcacca aacgtcaat aatgtcgac tttaccaat 
      ccttcaacc ccgtcttat tttaggctt acgaaccga 
      tgttaaagg caatgccca attcatcac agacgaaga 
      aataattta ccccgtggg ggcggtttt aaccatcca 
      caacctcaa ccaaaaacg ccaaaaata catcaaact 
      tgcagacaa gggtaaaaa tgtgactga agtggtggt 
      cagtcccaa caaaaaact agtcgccgt ggctgaaac 
      aggagaaaa acccttcat aaccctgtg gtgcccctt 
      aacttacaa aaagaaacg ccaaattaa ccttccaac 
      tgataagga aatttcagc tgagaaatt cacagcagt 
      tgcgtttac ttttgactc gaaatacat tgtttgtgt 
      gagtgggga acctgactg gaccttgta ctacttcag 
      gtgcgataa gggcaaact tgagagtag tgctagggc 
      taataatat tagcaatac ggggtatgt gaggcagat 
      cgcttgcaa tcccaacga tgctaatct tttagccct 
      ggttggtga tgggctaat ccgaatttt ggcttgtac 
      tgactacac ctggaggca gtatgggta cagcaaagg 
      cgatcagtg gaactttag ctcagccgt ttggttaac 
      acaggaccg gcttttggt gggtactct cgatgggcg 
      ccttttggt tattgaaag tggcgagtt gaaatttgt 
      tttttcggc cgctgattt agcagtgat ggacttgaa 
      accaaaaga cgagttgca acaatcgtc tcaaaccag 
      cctcaaagt tttgagtga cgacgaaat tgacccgaa 
      tgaggatta cagcatccg ctccctaat ccagtttcc 
      gcgcggttt tgcctttgg ttacctaac cggaaccgt 
      gcacttgta cgaaatgga aacccctca caaattcat 
      caaacgcaa catcttcaa aatccccga ccacacaat 
      aacccgcga gtacgagga agaaaccga gcagaaaat 
      gaccacagt caactgcgt ctccatcaa cccatcgca 
      gggcaagct catggtcac ttgcaggga gagccagat 
      ctacaacgc gactctctg gatgcaaga ctccgcaat 
      caccaccgc agtcgagtc aatgttcac cgaatatgg 
      gccagccct ccataacgg acccatcgg gagcatggc 
      ggtgtgcaa ctggaagcc catcctgct cacggctgg 
      tcgcaacga ccgtacttt gaaaatctg gaactacga 
      gacggagga actggagct gatgcagca gttccagga 
      cgatttgta cggagttgc cttgcaccc gagtggcct 
      ttatgccat tgttggctt ctcggacaa gttgcggtt 
      tctgactgt gatgatcga agagtttga aatgacgcg 
      agagtttaa catccgcgt ttgcaagat gtgtgtctt 
      cagcaggct ggggtactt gtttgctgc tgcgaacgg 
      gaacgttat tcaagttta ctcgagtat tactttcga 
      aatgttgta taacttgaa ggggcacaa tgggcgcat 
      ttttggcat gtcttggac ggccgatga ccggaagat 
      ggcgtcgtg tgggagcga gggggctgt gtatgagtg 
      ggtggtgca ggagtcgaa acggttgtc ggaaactat 
      aatgaagca gtgcgagtt taaaggagt tgcgattac 
      gacggatgg gaggtcgat ttttgtcgt ggcaaatga 
      cgggcatat cagggaatt gatgaattc gaacgtcca 
      tagagatgt tttggtcac aacggccgg tttggactc 
      gatggttat gtcgaaaat cgaccaaat gctgttcgt 
      ggctggaaa tggggggac tgtttacaa tattaagtt 
      gccactgtt ggaaaaagc cgagtatca cgagtatag 
      cattcatgc cgatgttgt cacacaaat gttagtgtc 
      tttcgacga caagtacct tttcaccgg ttcagatga 
      gggcacaat ttgcttttg gaagttgtt aaataccga 
      agggaaagc tatcaaaat ggataagga ttttcaacc 
      gtcgaagga tatcctcat ttctcgcca aatccttga 
      ggaacgaat tgcacaaac taagaatct tcaaatgag 
      gttgaagga actagagtc ggaatatgc ctatcagtt 
      aaggcaaaa tgacgtcgt caatagttt gaggatgaa 
      ggagataca ttctggtta ctgtcaagc aatcgagga 
      actgaagga aaaaaacga acaactcga aaatgacca 
      cgtgcaaga aatccacaa catcaatct agacatcaa 
      gcaaatgaa attggacca tgaggagtt cgtgcgcga 
      attggaggc caactacaa cgaaaaatt aattttcga 
      atacgagaa atacctccg actggagga aaaaatgga 
      aaagatgag acgctacta cgagaaaga gttagacga 
      cttgagaaa agcgaaaca agactcgga ggagataat 
      aacgaatgg gttcctcga aaaattgga ggagaaaca 
      gatgcagtt tgaggagtt ggttgaaca aaccgctcg 
      caaaaccaa agcccacga agtgatcaa acaacaaat 
      tgaagatga cgccgatcg agagattta cgaattgaa 
      ggcggccca tgccaaaga actcaaaga agagcaaga 
      gttgaacgt gaagattcg aggcgaaac ggcgatcgt 
      gaagaaaaa actgatttc atcgcagaa ggaaatcga 
      cgaattgaa aagcaaagt gcacacttt ggaccacga 
      ccatgccaa gttgaagaa aacaatcat caatcttga 
      gaaagatat cgtcgattt gaagaagga gatctcgga 
      acgcgacgc cacgattca agacaaaga aaaacgcat 
      ctttgattt gaagcggaa gaaccaaga gttggagaa 
      gtttaagtt catccttga cttcaagat caaagaact 
      gaaatcgca aatcgagcc cagggagca aatgattca 
      agaacaaac cgagcaaat caatgccat ggttaaaga 
      actcgagaa tttgcaacg agttatttt gagtcttga 
      tttacaact gcacgaatt gagggaaaa actttacgc 
      ttcggataa cgaagtgaa gaagcaaat tgcgaagaa 
      cgcagcgat gaaccgagc attgaagaa gatcaagac 
      tgatatttt cctagcgag tggtcagat tcagaatgt 
      cccacttct gattaaaac tgtgaagga aatgtacca 
      caagttcaa catcgacga agatttcga actgaccca 
      agctgaaga cacggaagt gaagagcga gtttctgag 
      acagaggga gtttttgga gaggacggt gacaagtct 
      tcagatgca agttaataa gagtacttc gactttgag 
      ttcggataa ggtgaagtt gactgagga gaacgccgt 
      tttgttggt cgagaccaa catgttgcg gaagcaact 
      caaagacga gcagaggag gaaggataa gttgcagtc 
      gattctcgg ggtgaacca gaagtattt gccgccgaa 
      agtcgcaat ggaacgggt gcaaaatgc gacagcaac 
      aagggagga aattaagaa ggagtaccg gaaaacgat 
      cgagtacaa tgagaagag tatgcaagc tttgaggga 
      agagaacga cagactctt gaataaaat tctagtcga 
      ggcgagtaa cacgacgga aatgtcgcc aacagctgg 
      tgaattgtc aatacagga ggataataa aaatgatta 
      ataaaacaa atcaacgtt tattaattt aacaataaa 
      gtaacattt tttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY