Status
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Gene information
General information
TC005932 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5392697..5407476
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005932
    iBeetle screen iB_04039

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    Comment: No phenotype found!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005932 RA
      MSTLKRDKS KSKSKVRLT DEQIESFKN EYSIDDHLI 
      SLRELEIRH NTNIKRGLT EIQAHERLL RDGPNCLEA 
      PPGKSRWLI LANFLFCGF NALLWLAVI MAFVTFAIT 
      KSQGKPAAE EQLYFATVV LIVILFTGF FSFYQEAAN 
      VAIIEGFQK LTPKSATVI RDGERRVIP SEDVVIGDL 
      VELKAGEWI PADVRLVKC QSLKVDNSA ITGESNPQS 
      RSSELSDPL AIESPNLAF FSTCAVEGS GMGVVIKRA 
      DDTLIGAIA NLATSLEKG TTPIRKEIN YFIKFITVL 
      AFGIGTIFF IVCIAYGYD FFTSFTYFI ALIIANVPE 
      GLPVTLTAC MTLTSKRMA SKNCLVKKL EAIETLGCT 
      SVICSDKTG TLTQNKMKV VHLYYDNQA YYVMVDGES 
      LERESQAFQ ALCQVAVLC SRATFVIGQ EHLPSNERE 
      TIGDASESA LLKCMEMLL GNVSTKRRD NPKVCEIPF 
      NSTNKYQVS IHRIKGQFI LLMKGAPER ILDRCATIL 
      RFEETSTLT QDIKNGIMR AINNLGLKG ERVLAFADL 
      QLPSGIYNS SYAFDPEKK NFPLTGLRF VGLISMMDP 
      PRPAVPDAV RKCKTAGIR VIMVTGDHP ITAAAIAKQ 
      VGILSQQSI TSYDIALRR DVSVSLVTD KEKSMCNAA 
      VITGSDLRE MTTTELQNN MLTYQEIVF ARTSPQQKL 
      KIVEAFQKL GHIVAVTGD GVNDSPALK KADIGIAMG 
      IAGTDVSKE AADMILLDD NFSSIVTGV EEGRLIFDN 
      LKKSIAYLL TSNVPEIVP FIAMVFINI PPVIGILAI 
      MVIDVGKSP KIMATPSNQ FHALGTDLW PAISLAYEK 
      AEADIMTRR PRDPFYDKL VNHRLILLT YAQIGVIQT 
      CASFASYFL CMMEHGFFW GLLVGLRHD WIDKDKIVI 
      DSYGQEWTF EERKILTRK CYSSFFLSI VLTQVADLI 
      ICKTRRLSL FQQGMTNWV LNAGIFVEL SIAALAVYC 
      PGLRMLLEF EPITLDILV PTLPFAIII FSFDEELRT 
      CEQPENAQV QLSDPSPPK KAKNSIDTH RILGCGAQF 
      IHEMGVLST NRRTCFFD
    CDS sequence
    • >TC005932 RA
      atgtcgacc ctaaagcgc gacaaaagc aaatccaaa 
      tcgaaggtc cgcttaacc gacgaacag atcgaatct 
      ttcaaaaac gaatattcg atcgatgac cacttaatc 
      agtcttcgc gaactcgaa atcagacac aacaccaac 
      atcaaacgc ggcctaacc gaaatccaa gcccacgag 
      cgcctcctc cgagacggc cccaactgt cttgaagcc 
      ccacccggg aaatcccgt tggttgatt ttggcaaac 
      tttctattt tgtggcttc aatgccttg ttatggctc 
      gcggtgatt atggctttt gtcactttt gccataacg 
      aagtcacag gggaagcca gcggctgag gaacaactc 
      tactttgcc accgttgtc ctgattgtg atcctcttc 
      actggattt tttagtttt taccaagag gccgccaac 
      gttgctatt attgaaggt tttcagaaa ttgacacca 
      aaatcagcc acggttata cgagacggc gaaaggcgt 
      gtcatacca agcgaggac gttgtgatt ggtgatttg 
      gtggagcta aaggcgggc gaatggata cccgccgat 
      gttagattg gtcaaatgt caatcattg aaggttgat 
      aacagcgca atcaccgga gaatccaac ccacagagt 
      cgctcctcg gaattatca gaccctttg gcaatagaa 
      tcgccaaat ttggccttt tttagcacg tgtgccgtc 
      gaggggagt ggcatgggc gtggttatc aaacgcgcc 
      gatgacact ctgattggt gcaatcgcc aacttggcc 
      acctccctc gagaaggga accacgccc atccggaaa 
      gaaatcaac tattttatc aaattcatc acggttttg 
      gctttcggg atcggtacg atttttttc atcgtttgt 
      atagcctac ggttatgat tttttcaca agttttacg 
      tattttatc gctttgatt attgcaaat gttccggaa 
      ggacttccg gttacttta actgcctgc atgacgctt 
      acttccaaa cgaatggca tcgaaaaat tgtttggtc 
      aagaaactg gaagctata gaaacgcta ggttgcact 
      agtgtcata tgttcggac aaaacaggg actttgacc 
      caaaacaag atgaaagtt gtccatctc tactatgac 
      aaccaagcg tattatgtc atggtggac ggggaatcc 
      cttgaaagg gagagccag gctttccag gccctgtgc 
      caggttgca gtgctttgt agccgggcg acgtttgtg 
      attggtcaa gagcatctc ccttcaaac gaacgggaa 
      acaataggg gatgcgagt gaatcggct ttgttgaaa 
      tgtatggaa atgttgttg gggaatgtg tcgacaaaa 
      cgaagggat aatcctaag gtgtgcgaa atccccttc 
      aattcgacc aacaaatac caagtttcg attcaccgg 
      atcaagggc caatttatc cttttgatg aaaggcgcc 
      cccgaacgc atcctggac cgttgtgcc acaatcctg 
      cgatttgag gaaacctca accctgacc caggacatc 
      aaaaacggg atcatgcgc gcgatcaac aatctggga 
      ttaaaaggc gaacgcgtt ctggctttc gccgatttg 
      cagctacct tcagggatt tacaacagt agttacgcc 
      ttcgatcct gagaaaaaa aacttccca ttgactgga 
      ttaaggttt gttgggttg atttcgatg atggaccct 
      ccacgtccc gcagttcct gatgctgtc cgtaaatgt 
      aaaactgca gggattagg gtcatcatg gttacgggg 
      gatcatccc atcacggct gctgcgata gcaaagcaa 
      gtcgggatt ttgtcgcag caaagtata acgtcttat 
      gacattgcg ttgcgaaga gacgtttca gtcagtttg 
      gttactgat aaggagaaa agtatgtgt aatgcggct 
      gttatcacc gggagtgat ttgagggaa atgacaacc 
      actgagcta cagaataat atgttgacg tatcaggag 
      attgtgttc gcgaggacg agtccgcaa caaaaattg 
      aaaattgtg gaggcgttt cagaagctg gggcatatt 
      gtggctgtg acgggggat ggggttaat gattctcca 
      gccttaaag aaggccgat attggcata gcgatggga 
      attgccggc acagacgtg tccaaagag gccgctgac 
      atgatcctc cttgacgac aatttttcg tcaatagtc 
      accggagta gaagagggt cgtctcatc ttcgacaac 
      ttaaaaaag agcattgcc tacctcctg acctcaaac 
      gtgcccgaa atagtgccg tttatcgcc atggtcttc 
      ataaacata ccccccgtg atcggtatt ttagccata 
      atggtcatc gatgttggt aaatccccg aaaattatg 
      gcgacccct tctaatcaa ttccacgcc ttagggacg 
      gacctatgg ccagccatc agcctcgcg tacgagaaa 
      gccgaagct gatataatg actcgccga ccccgcgac 
      cccttctac gacaaactc gtcaaccac cgcttgatt 
      ctgctgacg tacgcccaa attggggtc attcagacg 
      tgtgccagc ttcgcgtcg tattttctg tgcatgatg 
      gagcatggg tttttctgg ggtctcctc gtaggttta 
      agacacgat tggatcgat aaggataag atagttata 
      gattcgtat ggtcaagag tggacgttc gaagagcgg 
      aagatttta acgaggaag tgttatagt agttttttt 
      ctgtcgata gttttgaca caagtggct gatttgatc 
      atttgtaaa acgaggaga ctgtcgctg tttcagcaa 
      ggaatgacg aactgggtg ctaaatgcg gggattttt 
      gtcgagttg agtattgca gcgttggcg gtttattgc 
      ccagggttg aggatgctg ctggagttt gaaccgatc 
      actttggat attttggtg cccacttta ccgttcgcg 
      attattatt ttctcgttt gatgaagag ctccgcaca 
      tgtgaacaa cccgaaaac gctcaagtg cagttgagc 
      gacccttca ccacccaag aaagcaaaa aactcaatc 
      gacactcac cgcatcctc ggctgtgga gcacaattc 
      attcacgaa atgggagtt ttgagcaca aatcgtcgc 
      acgtgcttc ttcgactaa
    mRNA sequence
    • >TC005932 RA
      catcaaagt cattttgaa gtttcccca tgtcgaccc 
      taaagcgcg acaaaagca aatccaaat cgaaggtcc 
      gcttaaccg acgaacaga tcgaatctt tcaaaaacg 
      aatattcga tcgatgacc acttaatca gtcttcgcg 
      aactcgaaa tcagacaca acaccaaca tcaaacgcg 
      gcctaaccg aaatccaag cccacgagc gcctcctcc 
      gagacggcc ccaactgtc ttgaagccc cacccggga 
      aatcccgtt ggttgattt tggcaaact ttctatttt 
      gtggcttca atgccttgt tatggctcg cggtgatta 
      tggcttttg tcacttttg ccataacga agtcacagg 
      ggaagccag cggctgagg aacaactct actttgcca 
      ccgttgtcc tgattgtga tcctcttca ctggatttt 
      ttagttttt accaagagg ccgccaacg ttgctatta 
      ttgaaggtt ttcagaaat tgacaccaa aatcagcca 
      cggttatac gagacggcg aaaggcgtg tcataccaa 
      gcgaggacg ttgtgattg gtgatttgg tggagctaa 
      aggcgggcg aatggatac ccgccgatg ttagattgg 
      tcaaatgtc aatcattga aggttgata acagcgcaa 
      tcaccggag aatccaacc cacagagtc gctcctcgg 
      aattatcag accctttgg caatagaat cgccaaatt 
      tggcctttt ttagcacgt gtgccgtcg aggggagtg 
      gcatgggcg tggttatca aacgcgccg atgacactc 
      tgattggtg caatcgcca acttggcca cctccctcg 
      agaagggaa ccacgccca tccggaaag aaatcaact 
      attttatca aattcatca cggttttgg ctttcggga 
      tcggtacga tttttttca tcgtttgta tagcctacg 
      gttatgatt ttttcacaa gttttacgt attttatcg 
      ctttgatta ttgcaaatg ttccggaag gacttccgg 
      ttactttaa ctgcctgca tgacgctta cttccaaac 
      gaatggcat cgaaaaatt gtttggtca agaaactgg 
      aagctatag aaacgctag gttgcacta gtgtcatat 
      gttcggaca aaacaggga ctttgaccc aaaacaaga 
      tgaaagttg tccatctct actatgaca accaagcgt 
      attatgtca tggtggacg gggaatccc ttgaaaggg 
      agagccagg ctttccagg ccctgtgcc aggttgcag 
      tgctttgta gccgggcga cgtttgtga ttggtcaag 
      agcatctcc cttcaaacg aacgggaaa caatagggg 
      atgcgagtg aatcggctt tgttgaaat gtatggaaa 
      tgttgttgg ggaatgtgt cgacaaaac gaagggata 
      atcctaagg tgtgcgaaa tccccttca attcgacca 
      acaaatacc aagtttcga ttcaccgga tcaagggcc 
      aatttatcc ttttgatga aaggcgccc ccgaacgca 
      tcctggacc gttgtgcca caatcctgc gatttgagg 
      aaacctcaa ccctgaccc aggacatca aaaacggga 
      tcatgcgcg cgatcaaca atctgggat taaaaggcg 
      aacgcgttc tggctttcg ccgatttgc agctacctt 
      cagggattt acaacagta gttacgcct tcgatcctg 
      agaaaaaaa acttcccat tgactggat taaggtttg 
      ttgggttga tttcgatga tggaccctc cacgtcccg 
      cagttcctg atgctgtcc gtaaatgta aaactgcag 
      ggattaggg tcatcatgg ttacggggg atcatccca 
      tcacggctg ctgcgatag caaagcaag tcgggattt 
      tgtcgcagc aaagtataa cgtcttatg acattgcgt 
      tgcgaagag acgtttcag tcagtttgg ttactgata 
      aggagaaaa gtatgtgta atgcggctg ttatcaccg 
      ggagtgatt tgagggaaa tgacaacca ctgagctac 
      agaataata tgttgacgt atcaggaga ttgtgttcg 
      cgaggacga gtccgcaac aaaaattga aaattgtgg 
      aggcgtttc agaagctgg ggcatattg tggctgtga 
      cgggggatg gggttaatg attctccag ccttaaaga 
      aggccgata ttggcatag cgatgggaa ttgccggca 
      cagacgtgt ccaaagagg ccgctgaca tgatcctcc 
      ttgacgaca atttttcgt caatagtca ccggagtag 
      aagagggtc gtctcatct tcgacaact taaaaaaga 
      gcattgcct acctcctga cctcaaacg tgcccgaaa 
      tagtgccgt ttatcgcca tggtcttca taaacatac 
      cccccgtga tcggtattt tagccataa tggtcatcg 
      atgttggta aatccccga aaattatgg cgacccctt 
      ctaatcaat tccacgcct tagggacgg acctatggc 
      cagccatca gcctcgcgt acgagaaag ccgaagctg 
      atataatga ctcgccgac cccgcgacc ccttctacg 
      acaaactcg tcaaccacc gcttgattc tgctgacgt 
      acgcccaaa ttggggtca ttcagacgt gtgccagct 
      tcgcgtcgt attttctgt gcatgatgg agcatgggt 
      ttttctggg gtctcctcg taggtttaa gacacgatt 
      ggatcgata aggataaga tagttatag attcgtatg 
      gtcaagagt ggacgttcg aagagcgga agattttaa 
      cgaggaagt gttatagta gtttttttc tgtcgatag 
      ttttgacac aagtggctg atttgatca tttgtaaaa 
      cgaggagac tgtcgctgt ttcagcaag gaatgacga 
      actgggtgc taaatgcgg ggatttttg tcgagttga 
      gtattgcag cgttggcgg tttattgcc cagggttga 
      ggatgctgc tggagtttg aaccgatca ctttggata 
      ttttggtgc ccactttac cgttcgcga ttattattt 
      tctcgtttg atgaagagc tccgcacat gtgaacaac 
      ccgaaaacg ctcaagtgc agttgagcg acccttcac 
      cacccaaga aagcaaaaa actcaatcg acactcacc 
      gcatcctcg gctgtggag cacaattca ttcacgaaa 
      tgggagttt tgagcacaa atcgtcgca cgtgcttct 
      tcgactaac gccaaatgg ggaatgccg ctgcgtata 
      catatggtg tttggcggt ctccttttc ggtcggaag 
      gtctccctg ttgcttgtc ttgcacgta tgtgtaaag 
      ttggccgac ctcgacgat gtcacgaag agatgcaac 
      tgcaggaga agggccttt tcgaagaaa gtgctttcg 
      cgcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY