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Gene information
General information
TC005888 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5992043..5993193
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005888
    iBeetle screen iB_04031

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005888 RA
      MEPIFPKII KREAKDFHC FEKLEPYSC LNCNYKTSL 
      LSHLKTHET KTKTNSYKC GNCDFTAYS LLIILKHVS 
      VSCPKNNWY HCTHCTYKT RARKYLKNH IKYTHTPDP 
      NIHWFSCDL CPFRSKWKG NLKGHIAAK HQPPTPREA 
      MKCPQCPFT TTWKNSLRK HFCIRHTHL TTRQKHKCE 
      HCPFETIRS DSLKRHMLV KHTPCEDIR WYKCEICGL 
      RTKRADHLK RHKYETHGI PKIEQKNKI CSCTANDNS 
      FRCQLCPSH FSRSFNLTK HVKEKHGSN EIQGQIKCE 
      KCPFKTLWK KSLNVHVLL KHGASFHGN RCDFHGNR
    CDS sequence
    • >TC005888 RA
      atggaacca atttttcca aaaatcatt aaacgagaa 
      gcgaaagat ttccattgt tttgaaaag ttggaaccg 
      tacagttgc ctcaattgc aattacaaa acaagcctt 
      ttatcccac ttaaaaacc cacgaaacc aaaaccaaa 
      accaactca tacaaatgt ggcaattgt gattttaca 
      gcatattcc cttttaatt atcctcaaa cacgtttca 
      gtctcatgt cccaagaac aactggtac cactgtacc 
      cactgcact tacaaaacc cgcgctcgt aagtacctc 
      aaaaatcac ataaaatac acccacacc cctgacccc 
      aacatccat tggttctcc tgcgacctt tgccctttc 
      cgttcgaaa tggaaaggc aacttaaaa ggccacatc 
      gccgccaaa caccaaccc ccaacccct cgggaagca 
      atgaaatgt ccgcagtgt cccttcacc accacttgg 
      aagaattcg ctgcgaaag cacttctgc atcagacac 
      acacacctg acaacccgc caaaaacac aaatgtgag 
      cattgcccc tttgagaca atccgcagc gactcactc 
      aagaggcac atgctagta aagcacaca ccttgtgaa 
      gacattcgt tggtacaag tgtgagata tgcggcctt 
      agaaccaaa agggccgat caccttaaa cgacacaag 
      tacgaaacg cacggaatc ccaaaaatt gaacaaaaa 
      aataaaatt tgctcgtgt acagccaac gacaactcg 
      tttcgatgt caattgtgc ccttcacat ttctcacga 
      agcttcaat ttgacgaaa cacgtcaag gaaaaacat 
      ggcagcaac gagatacaa gggcaaatc aagtgcgaa 
      aagtgtcct ttcaaaacc ctttggaaa aagagcctc 
      aacgtccat gttttactc aaacatggc gccagtttc 
      catggcaat agatgcgat ttccatggc aatagatga
    mRNA sequence
    • >TC005888 RA
      cttatctct tttagacct gtagtttgg tacacataa 
      atagttctc ggaacgtac cctctaatg tcaaacgat 
      tgaggttag taaacaaaa tagatggaa ccaattttt 
      ccaaaaatc attaaacga gaagcgaaa gatttccat 
      tgttttgaa aagttggaa ccgtacagt tgcctcaat 
      tgcaattac aaaacaagc cttttatcc cacttaaaa 
      acccacgaa accaaaacc aaaaccaac tcatacaaa 
      tgtggcaat tgtgatttt acagcatat tccctttta 
      attatcctc aaacacgtt tcagtctca tgtcccaag 
      aacaactgg taccactgt acccactgc acttacaaa 
      acccgcgct cgtaagtac ctcaaaaat cacataaaa 
      tacacccac acccctgac cccaacatc cattggttc 
      tcctgcgac ctttgccct ttccgttcg aaatggaaa 
      ggcaactta aaaggccac atcgccgcc aaacaccaa 
      cccccaacc cctcgggaa gcaatgaaa tgtccgcag 
      tgtcccttc accaccact tggaagaat tcgctgcga 
      aagcacttc tgcatcaga cacacacac ctgacaacc 
      cgccaaaaa cacaaatgt gagcattgc ccctttgag 
      acaatccgc agcgactca ctcaagagg cacatgcta 
      gtaaagcac acaccttgt gaagacatt cgttggtac 
      aagtgtgag atatgcggc cttagaacc aaaagggcc 
      gatcacctt aaacgacac aagtacgaa acgcacgga 
      atcccaaaa attgaacaa aaaaataaa atttgctcg 
      tgtacagcc aacgacaac tcgtttcga tgtcaattg 
      tgcccttca catttctca cgaagcttc aatttgacg 
      aaacacgtc aaggaaaaa catggcagc aacgagata 
      caagggcaa atcaagtgc gaaaagtgt cctttcaaa 
      accctttgg aaaaagagc ctcaacgtc catgtttta 
      ctcaaacat ggcgccagt ttccatggc aatagatgc 
      gatttccat ggcaataga tgaagtttc catggatac 
      agcagccaa ctgacatgg taggggcgg atccaacca 
      tcaatttta atttttaat aaataatgt atattcgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY