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Gene information
General information
TC005879 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6054436..6057823
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005879
    iBeetle screen iB_04028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005879 RA
      MAENIPEIA QVIKDAKAA FKAEFKQEA EIVVFAPGR 
      VNLIGEHID YNDGFVMPM ALPLVTVIV GKKVSGEET 
      TIITTNPDA DPPKKATIE MPNKNALVK TIPPGPPKW 
      SNYVKGVIS NYVGETPPA FNALITSTV PTGGGLSSS 
      AALEVATYT FLDALVGPS AVMPTDKAL ACQKAEHDY 
      ANVPCGIMD QFISVLGKK DHALLIDCR SMTSTLIPV 
      ADPNVVVLI TNSNVKHEL TGSEYSSRR TQCQQAALL 
      LKKLSLRDA KLEDIEYLK SINASEETI KRARHAVTE 
      IQRTQEAAE ALKKKDFKK FGDLMVASH NSLRDDFEV 
      SCEELDQLV KLAMEVKGV YGSRMTGGG FGGCTVTLV 
      QKSAVNDVI AHVKKGYKG KPTFYICKP SDGAQIVAK
    CDS sequence
    • >TC005879 RA
      atggccgaa aacatcccc gaaatcgcg caagtgata 
      aaagacgca aaagccgct ttcaaagcc gaattcaaa 
      caagaggcg gaaatcgtc gttttcgca ccgggacga 
      gtgaattta attggggaa catatcgat tacaacgat 
      ggtttcgtc atgcccatg gccttgccc ttggtaaca 
      gtaatagtc gggaagaaa gtaagtggt gaagaaacc 
      acaattatc accacaaac cccgacgca gaccccccc 
      aaaaaagcc accatcgaa atgcccaac aaaaacgcg 
      ctggtcaaa accatcccc ccagggccc cccaaatgg 
      tcgaactac gtcaagggg gtgatttcg aactacgtg 
      ggggaaacc ccgcccgca tttaacgcg cttattacg 
      agcactgtg cccacaggg ggcggtttg tcgagtagt 
      gcggctttg gaggtcgca acttatact tttctggac 
      gctttggtc ggccctagt gccgtaatg ccgactgat 
      aaggcactg gcgtgccag aaagccgaa catgactat 
      gctaacgtc ccgtgtggt ataatggac cagtttatt 
      tccgttttg ggtaaaaag gaccacgcg cttttgatc 
      gattgtcga agtatgact tcgactctg atcccggtc 
      gctgatcca aatgtggtc gttttgatc acgaattca 
      aatgttaaa cacgaattg acgggcagt gagtactct 
      tcaaggaga actcaatgt caacaagcc gcactattg 
      ctgaaaaaa ttgagcttg agggacgcc aagcttgaa 
      gatattgaa tatttgaaa tcgataaat gcgtccgag 
      gagacaata aaaagggcg cgacatgcc gtcactgaa 
      atacaacgc acgcaagaa gcggccgaa gcgctcaaa 
      aagaaagat tttaaaaaa tttggtgac ttgatggtt 
      gcgagtcac aattcgtta cgagatgat tttgaagtg 
      tcgtgtgaa gaattagac caattggtt aaattagca 
      atggaggtc aagggggtt tacgggagt agaatgaca 
      gggggcgga tttgggggc tgcactgtc acacttgtg 
      caaaaatca gccgttaat gatgttata gctcacgtt 
      aagaagggc tacaaagga aaacctact ttttatatc 
      tgcaaaccg agcgatgga gcacaaatt gtggccaaa 
      tga
    mRNA sequence
    • >TC005879 RA
      tcaaaacta aatatttac ctttaatat ctttaatta 
      acgggtcac tgactgaca ttgttatct ccaagtcct 
      atcattcag ctattttcg aataatttt atcaaatat 
      cacgccaaa cacacgtcg ttcaacatt ttcagtgac 
      ttttcctca ccgacacgt caaaatttc taaaatttt 
      ccgcaagaa atggccgaa aacatcccc gaaatcgcg 
      caagtgata aaagacgca aaagccgct ttcaaagcc 
      gaattcaaa caagaggcg gaaatcgtc gttttcgca 
      ccgggacga gtgaattta attggggaa catatcgat 
      tacaacgat ggtttcgtc atgcccatg gccttgccc 
      ttggtaaca gtaatagtc gggaagaaa gtaagtggt 
      gaagaaacc acaattatc accacaaac cccgacgca 
      gaccccccc aaaaaagcc accatcgaa atgcccaac 
      aaaaacgcg ctggtcaaa accatcccc ccagggccc 
      cccaaatgg tcgaactac gtcaagggg gtgatttcg 
      aactacgtg ggggaaacc ccgcccgca tttaacgcg 
      cttattacg agcactgtg cccacaggg ggcggtttg 
      tcgagtagt gcggctttg gaggtcgca acttatact 
      tttctggac gctttggtc ggccctagt gccgtaatg 
      ccgactgat aaggcactg gcgtgccag aaagccgaa 
      catgactat gctaacgtc ccgtgtggt ataatggac 
      cagtttatt tccgttttg ggtaaaaag gaccacgcg 
      cttttgatc gattgtcga agtatgact tcgactctg 
      atcccggtc gctgatcca aatgtggtc gttttgatc 
      acgaattca aatgttaaa cacgaattg acgggcagt 
      gagtactct tcaaggaga actcaatgt caacaagcc 
      gcactattg ctgaaaaaa ttgagcttg agggacgcc 
      aagcttgaa gatattgaa tatttgaaa tcgataaat 
      gcgtccgag gagacaata aaaagggcg cgacatgcc 
      gtcactgaa atacaacgc acgcaagaa gcggccgaa 
      gcgctcaaa aagaaagat tttaaaaaa tttggtgac 
      ttgatggtt gcgagtcac aattcgtta cgagatgat 
      tttgaagtg tcgtgtgaa gaattagac caattggtt 
      aaattagca atggaggtc aagggggtt tacgggagt 
      agaatgaca gggggcgga tttgggggc tgcactgtc 
      acacttgtg caaaaatca gccgttaat gatgttata 
      gctcacgtt aagaagggc tacaaagga aaacctact 
      ttttatatc tgcaaaccg agcgatgga gcacaaatt 
      gtggccaaa tgagatatt tatacacta tattatgta 
      ttataagtg tttataaat tttataccc aatttttgc 
      gttattgct atataccta tatacctaa accg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY