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Gene information
General information
TC034019
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005871,TC005872
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6138791..6143602
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034019
    iBeetle screen iB_04026

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034019 RA
      MSENFDPPP KLPDREDAN APQPGDTPN ERRTSDEKR 
      RVKVSLPEI ELNQAFKEE KPDDYLSLK SVSLSSIWS 
      ILKKDAERT IKEETEIDR DLLFASAGS EKSAESSRK 
      TVSPPRSIR SEHVSGPEM SDVYEQPME HDDISYAIP 
      EDFITKTRR STVSSSIHF VSDDFVKCV KSLDMATIA 
      EEDETREFG DEDIEDIAI DRKLYSELY TIFEIRRYK 
      EKLINNYLQ ARLAKYYMR RKMFYVLRE MTPKVDEVI 
      AKYHKDLES LEHLCDSAE RDKALNNEE IKNLTLKRD 
      AKVVDLETL FKNLIHMEQ EFAKTLTLE KTGRTNVDK 
      MTERFVRRQ KNQTNLLIK LRMSYINLR NRFNEKMTT 
      YENLDSLGP GYGIMDYVQ LKADNRKLY DILEEKEAE 
      LTKLRLACK DTIQVLAHN REKSQVVNL DIEKLKEEL 
      QVTETELND AREQLNYLK RERDNYRKI IKNIRLEYG 
      LLTNPKLLR DMESAINEI DVLKDDIER TKNKYFLKR 
      KQTEAVRRN MELYLAERK REEGRRLKI LESVSSLEI 
      KKKPTLVKK KSEPGMGFG SKKSVSIVD LPKRKKD
    CDS sequence
    • >TC034019 RA
      atgtcggaa aattttgac cctccacca aaattaccc 
      gaccgtgaa gatgcaaac gcccctcaa ccaggggat 
      acccccaac gaacgccgc acttcggac gagaaacgc 
      cgagttaaa gtgtctctc ccggaaatc gaattaaac 
      caagcattc aaagaggaa aaaccggac gattactta 
      tcactcaag agtgttagt ttgagttcg atttggtca 
      attttgaaa aaagacgcg gagcgaacg atcaaagag 
      gaaaccgaa attgaccga gacctgctt tttgcgtcc 
      gccggttcg gaaaaaagc gcagaatcc tcaagaaaa 
      accgtatct cctccacgg agcatcaga tctgaacac 
      gtttccgga ccggaaatg tcggatgtt tacgaacaa 
      cctatggaa cacgatgat attagttat gccattccg 
      gaggatttt attacgaaa acgcgcaga tcaacggtc 
      tcgtccagc attcacttc gtctcagac gacttcgta 
      aagtgcgta aagtcgcta gatatggcc acgatagcc 
      gaagaagat gaaacgaga gaattcggc gatgaagac 
      attgaagac atagcaatt gatagaaag ttgtacagt 
      gaactgtac actatattt gagataaga aggtacaag 
      gaaaaactc ataaataac tatctgcaa gcgagactc 
      gccaaatat tacatgagg cgtaaaatg ttttacgtt 
      ttgagagaa atgacgccg aaagtggac gaagttatt 
      gcaaagtat cataaagac ttggaaagt ttggaacat 
      ttgtgtgat tcagccgaa cgagacaag gcactcaat 
      aacgaagaa atcaaaaat ttgaccttg aaacgtgac 
      gctaaagtg gtcgatttg gaaacactg ttcaaaaat 
      ttgatacat atggaacag gaatttgcg aaaacgctc 
      actttggaa aaaaccggg cgcactaac gtcgacaag 
      atgacggag cgttttgtg aggaggcaa aaaaatcag 
      acgaatctt ttgataaaa ttacgaatg agttacatc 
      aatttacgg aatcgattc aacgaaaaa atgacgact 
      tacgaaaat ttggattct ttgggtccg ggttacgga 
      attatggat tacgtacag ttgaaggcc gacaatcga 
      aaattgtac gacattttg gaagagaag gaagcggaa 
      ttaacgaaa ttacgtctg gcttgtaag gacacgatt 
      caggttttg gcacacaat cgtgagaaa tcgcaagtc 
      gtcaatttg gatattgag aaattgaag gaagagtta 
      caagttaca gagacggag cttaacgat gcgcgcgaa 
      caactgaac tatttgaag cgcgagcga gataattat 
      aggaaaata ataaaaaat attcgtctt gaatacggt 
      ttgttgacg aatcccaaa ctcctacgt gatatggaa 
      agcgcaata aatgaaatt gacgtcttg aaggatgat 
      atcgaaagg acgaaaaat aaatatttc ctgaaacga 
      aaacaaacc gaagcggtt cgtcgaaac atggagctg 
      tatttggcg gagagaaag cgtgaagaa ggccgccga 
      ttgaaaatt ttggagagt gttagtagt ttagaaatt 
      aaaaagaaa ccgacactt gtcaagaaa aagagcgaa 
      ccggggatg ggtttcgga tcgaaaaag agcgtctca 
      attgtagac ttacccaaa agaaagaaa gattaa
    mRNA sequence
    • >TC034019 RA
      tgaaccatt tttgctcta aacagccat aattttcgt 
      aaaattttt acgtctagt gggccataa atagtcata 
      attatgtca aatttgact ctagtttag gtagtttta 
      cattttaga tttacttct gtagtgtag agtgtagaa 
      ataccatgt cggaaaatt ttgaccctc caccaaaat 
      tacccgacc gtgaagatg caaacgccc ctcaaccag 
      gggataccc ccaacgaac gccgcactt cggacgaga 
      aacgccgag ttaaagtgt ctctcccgg aaatcgaat 
      taaaccaag cattcaaag aggaaaaac cggacgatt 
      acttatcac tcaagagtg ttagtttga gttcgattt 
      ggtcaattt tgaaaaaag acgcggagc gaacgatca 
      aagaggaaa ccgaaattg accgagacc tgctttttg 
      cgtccgccg gttcggaaa aaagcgcag aatcctcaa 
      gaaaaaccg tatctcctc cacggagca tcagatctg 
      aacacgttt ccggaccgg aaatgtcgg atgtttacg 
      aacaaccta tggaacacg atgatatta gttatgcca 
      ttccggagg attttatta cgaaaacgc gcagatcaa 
      cggtctcgt ccagcattc acttcgtct cagacgact 
      tcgtaaagt gcgtaaagt cgctagata tggccacga 
      tagccgaag aagatgaaa cgagagaat tcggcgatg 
      aagacattg aagacatag caattgata gaaagttgt 
      acagtgaac tgtacacta tatttgaga taagaaggt 
      acaaggaaa aactcataa ataactatc tgcaagcga 
      gactcgcca aatattaca tgaggcgta aaatgtttt 
      acgttttga gagaaatga cgccgaaag tggacgaag 
      ttattgcaa agtatcata aagacttgg aaagtttgg 
      aacatttgt gtgattcag ccgaacgag acaaggcac 
      tcaataacg aagaaatca aaaatttga ccttgaaac 
      gtgacgcta aagtggtcg atttggaaa cactgttca 
      aaaatttga tacatatgg aacaggaat ttgcgaaaa 
      cgctcactt tggaaaaaa ccgggcgca ctaacgtcg 
      acaagatga cggagcgtt ttgtgagga ggcaaaaaa 
      atcagacga atcttttga taaaattac gaatgagtt 
      acatcaatt tacggaatc gattcaacg aaaaaatga 
      cgacttacg aaaatttgg attctttgg gtccgggtt 
      acggaatta tggattacg tacagttga aggccgaca 
      atcgaaaat tgtacgaca ttttggaag agaaggaag 
      cggaattaa cgaaattac gtctggctt gtaaggaca 
      cgattcagg ttttggcac acaatcgtg agaaatcgc 
      aagtcgtca atttggata ttgagaaat tgaaggaag 
      agttacaag ttacagaga cggagctta acgatgcgc 
      gcgaacaac tgaactatt tgaagcgcg agcgagata 
      attatagga aaataataa aaaatattc gtcttgaat 
      acggtttgt tgacgaatc ccaaactcc tacgtgata 
      tggaaagcg caataaatg aaattgacg tcttgaagg 
      atgatatcg aaaggacga aaaataaat atttcctga 
      aacgaaaac aaaccgaag cggttcgtc gaaacatgg 
      agctgtatt tggcggaga gaaagcgtg aagaaggcc 
      gccgattga aaattttgg agagtgtta gtagtttag 
      aaattaaaa agaaaccga cacttgtca agaaaaaga 
      gcgaaccgg ggatgggtt tcggatcga aaaagagcg 
      tctcaattg tagacttac ccaaaagaa agaaagatt 
      aattgattt tttaataaa taaaacaat tcattccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY