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Gene information
General information
TC034019
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005871,TC005872 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6138791..6143602
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034019
    iBeetle screen iB_04026

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034019 RA
      MSENFDPPP KLPDREDAN APQPGDTPN ERRTSDEKR 
RVKVSLPEI ELNQAFKEE KPDDYLSLK SVSLSSIWS 
ILKKDAERT IKEETEIDR DLLFASAGS EKSAESSRK 
TVSPPRSIR SEHVSGPEM SDVYEQPME HDDISYAIP 
EDFITKTRR STVSSSIHF VSDDFVKCV KSLDMATIA 
EEDETREFG DEDIEDIAI DRKLYSELY TIFEIRRYK 
EKLINNYLQ ARLAKYYMR RKMFYVLRE MTPKVDEVI 
AKYHKDLES LEHLCDSAE RDKALNNEE IKNLTLKRD 
AKVVDLETL FKNLIHMEQ EFAKTLTLE KTGRTNVDK 
MTERFVRRQ KNQTNLLIK LRMSYINLR NRFNEKMTT 
YENLDSLGP GYGIMDYVQ LKADNRKLY DILEEKEAE 
LTKLRLACK DTIQVLAHN REKSQVVNL DIEKLKEEL 
QVTETELND AREQLNYLK RERDNYRKI IKNIRLEYG 
LLTNPKLLR DMESAINEI DVLKDDIER TKNKYFLKR 
KQTEAVRRN MELYLAERK REEGRRLKI LESVSSLEI 
KKKPTLVKK KSEPGMGFG SKKSVSIVD LPKRKKD
    CDS sequence
    • >TC034019 RA
      atgtcggaa aattttgac cctccacca aaattaccc 
gaccgtgaa gatgcaaac gcccctcaa ccaggggat 
acccccaac gaacgccgc acttcggac gagaaacgc 
cgagttaaa gtgtctctc ccggaaatc gaattaaac 
caagcattc aaagaggaa aaaccggac gattactta 
tcactcaag agtgttagt ttgagttcg atttggtca 
attttgaaa aaagacgcg gagcgaacg atcaaagag 
gaaaccgaa attgaccga gacctgctt tttgcgtcc 
gccggttcg gaaaaaagc gcagaatcc tcaagaaaa 
accgtatct cctccacgg agcatcaga tctgaacac 
gtttccgga ccggaaatg tcggatgtt tacgaacaa 
cctatggaa cacgatgat attagttat gccattccg 
gaggatttt attacgaaa acgcgcaga tcaacggtc 
tcgtccagc attcacttc gtctcagac gacttcgta 
aagtgcgta aagtcgcta gatatggcc acgatagcc 
gaagaagat gaaacgaga gaattcggc gatgaagac 
attgaagac atagcaatt gatagaaag ttgtacagt 
gaactgtac actatattt gagataaga aggtacaag 
gaaaaactc ataaataac tatctgcaa gcgagactc 
gccaaatat tacatgagg cgtaaaatg ttttacgtt 
ttgagagaa atgacgccg aaagtggac gaagttatt 
gcaaagtat cataaagac ttggaaagt ttggaacat 
ttgtgtgat tcagccgaa cgagacaag gcactcaat 
aacgaagaa atcaaaaat ttgaccttg aaacgtgac 
gctaaagtg gtcgatttg gaaacactg ttcaaaaat 
ttgatacat atggaacag gaatttgcg aaaacgctc 
actttggaa aaaaccggg cgcactaac gtcgacaag 
atgacggag cgttttgtg aggaggcaa aaaaatcag 
acgaatctt ttgataaaa ttacgaatg agttacatc 
aatttacgg aatcgattc aacgaaaaa atgacgact 
tacgaaaat ttggattct ttgggtccg ggttacgga 
attatggat tacgtacag ttgaaggcc gacaatcga 
aaattgtac gacattttg gaagagaag gaagcggaa 
ttaacgaaa ttacgtctg gcttgtaag gacacgatt 
caggttttg gcacacaat cgtgagaaa tcgcaagtc 
gtcaatttg gatattgag aaattgaag gaagagtta 
caagttaca gagacggag cttaacgat gcgcgcgaa 
caactgaac tatttgaag cgcgagcga gataattat 
aggaaaata ataaaaaat attcgtctt gaatacggt 
ttgttgacg aatcccaaa ctcctacgt gatatggaa 
agcgcaata aatgaaatt gacgtcttg aaggatgat 
atcgaaagg acgaaaaat aaatatttc ctgaaacga 
aaacaaacc gaagcggtt cgtcgaaac atggagctg 
tatttggcg gagagaaag cgtgaagaa ggccgccga 
ttgaaaatt ttggagagt gttagtagt ttagaaatt 
aaaaagaaa ccgacactt gtcaagaaa aagagcgaa 
ccggggatg ggtttcgga tcgaaaaag agcgtctca 
attgtagac ttacccaaa agaaagaaa gattaa
    mRNA sequence
    • >TC034019 RA
      tgaaccatt tttgctcta aacagccat aattttcgt 
aaaattttt acgtctagt gggccataa atagtcata 
attatgtca aatttgact ctagtttag gtagtttta 
cattttaga tttacttct gtagtgtag agtgtagaa 
ataccatgt cggaaaatt ttgaccctc caccaaaat 
tacccgacc gtgaagatg caaacgccc ctcaaccag 
gggataccc ccaacgaac gccgcactt cggacgaga 
aacgccgag ttaaagtgt ctctcccgg aaatcgaat 
taaaccaag cattcaaag aggaaaaac cggacgatt 
acttatcac tcaagagtg ttagtttga gttcgattt 
ggtcaattt tgaaaaaag acgcggagc gaacgatca 
aagaggaaa ccgaaattg accgagacc tgctttttg 
cgtccgccg gttcggaaa aaagcgcag aatcctcaa 
gaaaaaccg tatctcctc cacggagca tcagatctg 
aacacgttt ccggaccgg aaatgtcgg atgtttacg 
aacaaccta tggaacacg atgatatta gttatgcca 
ttccggagg attttatta cgaaaacgc gcagatcaa 
cggtctcgt ccagcattc acttcgtct cagacgact 
tcgtaaagt gcgtaaagt cgctagata tggccacga 
tagccgaag aagatgaaa cgagagaat tcggcgatg 
aagacattg aagacatag caattgata gaaagttgt 
acagtgaac tgtacacta tatttgaga taagaaggt 
acaaggaaa aactcataa ataactatc tgcaagcga 
gactcgcca aatattaca tgaggcgta aaatgtttt 
acgttttga gagaaatga cgccgaaag tggacgaag 
ttattgcaa agtatcata aagacttgg aaagtttgg 
aacatttgt gtgattcag ccgaacgag acaaggcac 
tcaataacg aagaaatca aaaatttga ccttgaaac 
gtgacgcta aagtggtcg atttggaaa cactgttca 
aaaatttga tacatatgg aacaggaat ttgcgaaaa 
cgctcactt tggaaaaaa ccgggcgca ctaacgtcg 
acaagatga cggagcgtt ttgtgagga ggcaaaaaa 
atcagacga atcttttga taaaattac gaatgagtt 
acatcaatt tacggaatc gattcaacg aaaaaatga 
cgacttacg aaaatttgg attctttgg gtccgggtt 
acggaatta tggattacg tacagttga aggccgaca 
atcgaaaat tgtacgaca ttttggaag agaaggaag 
cggaattaa cgaaattac gtctggctt gtaaggaca 
cgattcagg ttttggcac acaatcgtg agaaatcgc 
aagtcgtca atttggata ttgagaaat tgaaggaag 
agttacaag ttacagaga cggagctta acgatgcgc 
gcgaacaac tgaactatt tgaagcgcg agcgagata 
attatagga aaataataa aaaatattc gtcttgaat 
acggtttgt tgacgaatc ccaaactcc tacgtgata 
tggaaagcg caataaatg aaattgacg tcttgaagg 
atgatatcg aaaggacga aaaataaat atttcctga 
aacgaaaac aaaccgaag cggttcgtc gaaacatgg 
agctgtatt tggcggaga gaaagcgtg aagaaggcc 
gccgattga aaattttgg agagtgtta gtagtttag 
aaattaaaa agaaaccga cacttgtca agaaaaaga 
gcgaaccgg ggatgggtt tcggatcga aaaagagcg 
tctcaattg tagacttac ccaaaagaa agaaagatt 
aattgattt tttaataaa taaaacaat tcattccat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY