| General information | |||
| TC034019
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 6138791..6143602 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034019 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04026 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (16 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC034019 RA
MSENFDPPP KLPDREDAN APQPGDTPN ERRTSDEKR RVKVSLPEI ELNQAFKEE KPDDYLSLK SVSLSSIWS ILKKDAERT IKEETEIDR DLLFASAGS EKSAESSRK TVSPPRSIR SEHVSGPEM SDVYEQPME HDDISYAIP EDFITKTRR STVSSSIHF VSDDFVKCV KSLDMATIA EEDETREFG DEDIEDIAI DRKLYSELY TIFEIRRYK EKLINNYLQ ARLAKYYMR RKMFYVLRE MTPKVDEVI AKYHKDLES LEHLCDSAE RDKALNNEE IKNLTLKRD AKVVDLETL FKNLIHMEQ EFAKTLTLE KTGRTNVDK MTERFVRRQ KNQTNLLIK LRMSYINLR NRFNEKMTT YENLDSLGP GYGIMDYVQ LKADNRKLY DILEEKEAE LTKLRLACK DTIQVLAHN REKSQVVNL DIEKLKEEL QVTETELND AREQLNYLK RERDNYRKI IKNIRLEYG LLTNPKLLR DMESAINEI DVLKDDIER TKNKYFLKR KQTEAVRRN MELYLAERK REEGRRLKI LESVSSLEI KKKPTLVKK KSEPGMGFG SKKSVSIVD LPKRKKD
- >TC034019 RA
atgtcggaa aattttgac cctccacca aaattaccc gaccgtgaa gatgcaaac gcccctcaa ccaggggat acccccaac gaacgccgc acttcggac gagaaacgc cgagttaaa gtgtctctc ccggaaatc gaattaaac caagcattc aaagaggaa aaaccggac gattactta tcactcaag agtgttagt ttgagttcg atttggtca attttgaaa aaagacgcg gagcgaacg atcaaagag gaaaccgaa attgaccga gacctgctt tttgcgtcc gccggttcg gaaaaaagc gcagaatcc tcaagaaaa accgtatct cctccacgg agcatcaga tctgaacac gtttccgga ccggaaatg tcggatgtt tacgaacaa cctatggaa cacgatgat attagttat gccattccg gaggatttt attacgaaa acgcgcaga tcaacggtc tcgtccagc attcacttc gtctcagac gacttcgta aagtgcgta aagtcgcta gatatggcc acgatagcc gaagaagat gaaacgaga gaattcggc gatgaagac attgaagac atagcaatt gatagaaag ttgtacagt gaactgtac actatattt gagataaga aggtacaag gaaaaactc ataaataac tatctgcaa gcgagactc gccaaatat tacatgagg cgtaaaatg ttttacgtt ttgagagaa atgacgccg aaagtggac gaagttatt gcaaagtat cataaagac ttggaaagt ttggaacat ttgtgtgat tcagccgaa cgagacaag gcactcaat aacgaagaa atcaaaaat ttgaccttg aaacgtgac gctaaagtg gtcgatttg gaaacactg ttcaaaaat ttgatacat atggaacag gaatttgcg aaaacgctc actttggaa aaaaccggg cgcactaac gtcgacaag atgacggag cgttttgtg aggaggcaa aaaaatcag acgaatctt ttgataaaa ttacgaatg agttacatc aatttacgg aatcgattc aacgaaaaa atgacgact tacgaaaat ttggattct ttgggtccg ggttacgga attatggat tacgtacag ttgaaggcc gacaatcga aaattgtac gacattttg gaagagaag gaagcggaa ttaacgaaa ttacgtctg gcttgtaag gacacgatt caggttttg gcacacaat cgtgagaaa tcgcaagtc gtcaatttg gatattgag aaattgaag gaagagtta caagttaca gagacggag cttaacgat gcgcgcgaa caactgaac tatttgaag cgcgagcga gataattat aggaaaata ataaaaaat attcgtctt gaatacggt ttgttgacg aatcccaaa ctcctacgt gatatggaa agcgcaata aatgaaatt gacgtcttg aaggatgat atcgaaagg acgaaaaat aaatatttc ctgaaacga aaacaaacc gaagcggtt cgtcgaaac atggagctg tatttggcg gagagaaag cgtgaagaa ggccgccga ttgaaaatt ttggagagt gttagtagt ttagaaatt aaaaagaaa ccgacactt gtcaagaaa aagagcgaa ccggggatg ggtttcgga tcgaaaaag agcgtctca attgtagac ttacccaaa agaaagaaa gattaa
- >TC034019 RA
tgaaccatt tttgctcta aacagccat aattttcgt aaaattttt acgtctagt gggccataa atagtcata attatgtca aatttgact ctagtttag gtagtttta cattttaga tttacttct gtagtgtag agtgtagaa ataccatgt cggaaaatt ttgaccctc caccaaaat tacccgacc gtgaagatg caaacgccc ctcaaccag gggataccc ccaacgaac gccgcactt cggacgaga aacgccgag ttaaagtgt ctctcccgg aaatcgaat taaaccaag cattcaaag aggaaaaac cggacgatt acttatcac tcaagagtg ttagtttga gttcgattt ggtcaattt tgaaaaaag acgcggagc gaacgatca aagaggaaa ccgaaattg accgagacc tgctttttg cgtccgccg gttcggaaa aaagcgcag aatcctcaa gaaaaaccg tatctcctc cacggagca tcagatctg aacacgttt ccggaccgg aaatgtcgg atgtttacg aacaaccta tggaacacg atgatatta gttatgcca ttccggagg attttatta cgaaaacgc gcagatcaa cggtctcgt ccagcattc acttcgtct cagacgact tcgtaaagt gcgtaaagt cgctagata tggccacga tagccgaag aagatgaaa cgagagaat tcggcgatg aagacattg aagacatag caattgata gaaagttgt acagtgaac tgtacacta tatttgaga taagaaggt acaaggaaa aactcataa ataactatc tgcaagcga gactcgcca aatattaca tgaggcgta aaatgtttt acgttttga gagaaatga cgccgaaag tggacgaag ttattgcaa agtatcata aagacttgg aaagtttgg aacatttgt gtgattcag ccgaacgag acaaggcac tcaataacg aagaaatca aaaatttga ccttgaaac gtgacgcta aagtggtcg atttggaaa cactgttca aaaatttga tacatatgg aacaggaat ttgcgaaaa cgctcactt tggaaaaaa ccgggcgca ctaacgtcg acaagatga cggagcgtt ttgtgagga ggcaaaaaa atcagacga atcttttga taaaattac gaatgagtt acatcaatt tacggaatc gattcaacg aaaaaatga cgacttacg aaaatttgg attctttgg gtccgggtt acggaatta tggattacg tacagttga aggccgaca atcgaaaat tgtacgaca ttttggaag agaaggaag cggaattaa cgaaattac gtctggctt gtaaggaca cgattcagg ttttggcac acaatcgtg agaaatcgc aagtcgtca atttggata ttgagaaat tgaaggaag agttacaag ttacagaga cggagctta acgatgcgc gcgaacaac tgaactatt tgaagcgcg agcgagata attatagga aaataataa aaaatattc gtcttgaat acggtttgt tgacgaatc ccaaactcc tacgtgata tggaaagcg caataaatg aaattgacg tcttgaagg atgatatcg aaaggacga aaaataaat atttcctga aacgaaaac aaaccgaag cggttcgtc gaaacatgg agctgtatt tggcggaga gaaagcgtg aagaaggcc gccgattga aaattttgg agagtgtta gtagtttag aaattaaaa agaaaccga cacttgtca agaaaaaga gcgaaccgg ggatgggtt tcggatcga aaaagagcg tctcaattg tagacttac ccaaaagaa agaaagatt aattgattt tttaataaa taaaacaat tcattccat
