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Gene information
General information
TC005870 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6145848..6150322
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005870
      iBeetle screen iB_04025

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005870 RA
        MSKLTVRLV NVIKLFSFH HDNLRTLGD RVIKLQFLT 
        LFKAITNFG LPPNGEDKV TKYWTGKSV DEIIAEADL 
        LFDQGKYLQ VYELLNRLK FCNNIEVQW RIGRALFKL 
        SHEHEITED IRREMIDEA YEIIQSSLT VAENSPIIH 
        KWAAIIIDR KNGMKGLEH RVKNSEVVK HHLIRTCEL 
        DPDDVTAQY LLGRWCYEM SNITWFQRI IAKLLYGEA 
        PRSSFEEAN KYLSRAEDL HPRFYLQNT YLLGKTCLK 
        LGQYYRAKH YFGVVGNLP VHNEYERHC ANDAKKLLK 
        RLDKYSLEK NALFYEYPF GTNE
      CDS sequence
      • >TC005870 RA
        atgtcaaaa ttaactgta cgtctcgtg aacgtaata 
        aaactcttc tctttccac cacgataat ttgcgaact 
        ttgggcgac cgtgtgata aaattgcaa tttttgact 
        ctattcaaa gccataacg aatttcggt ttgccccca 
        aatggcgaa gacaaagtg acgaaatac tggactggt 
        aaatcagtc gacgaaatc atcgccgaa gccgatctc 
        ctcttcgac caaggcaaa tacttgcaa gtttacgaa 
        ttactaaac cgtttgaaa ttctgcaac aacatcgaa 
        gttcaatgg cgaatcggt cgagccctc tttaaacta 
        tcacacgaa catgaaatc accgaagat atccggcgc 
        gaaatgatt gacgaagcc tacgagatc atccaaagc 
        agtcttaca gttgcggaa aattccccg ataatccac 
        aaatgggcc gcaatcatt atcgatcgg aaaaacggg 
        atgaaagga ctcgaacat cgagtcaaa aattcggaa 
        gttgtcaaa caccacctc atcagaacg tgcgaactg 
        gaccccgac gacgtcacc gcgcagtat cttctaggt 
        cggtggtgc tacgaaatg agcaacatc acgtggttt 
        caacgcatc attgcgaaa ctgttgtac ggggaagcc 
        ccgagatcg agcttcgaa gaagccaac aagtattta 
        tcacgtgct gaagacttg cacccccga ttttaccta 
        caaaatacg tatttattg ggcaaaact tgcctaaaa 
        ttagggcaa tactaccgg gccaaacac tatttcgga 
        gtcgtgggt aatttaccc gtccataac gagtacgag 
        aggcattgt gcgaacgac gcgaaaaaa ttactcaaa 
        cgtttagac aagtacagt ttggagaaa aacgccctt 
        ttttacgaa taccccttc ggaaccaac gagtaa
      mRNA sequence
      • >TC005870 RA
        aaaacttcc gcagtgtta tcagtgcaa aaaatatgg 
        gtcaaggtc ccattttgc atagttatg gcaaccaaa 
        aaccattga caaatatga gaaatgtca aaattaact 
        gtacgtctc gtgaacgta ataaaactc ttctctttc 
        caccacgat aatttgcga actttgggc gaccgtgtg 
        ataaaattg caatttttg actctattc aaagccata 
        acgaatttc ggtttgccc ccaaatggc gaagacaaa 
        gtgacgaaa tactggact ggtaaatca gtcgacgaa 
        atcatcgcc gaagccgat ctcctcttc gaccaaggc 
        aaatacttg caagtttac gaattacta aaccgtttg 
        aaattctgc aacaacatc gaagttcaa tggcgaatc 
        ggtcgagcc ctctttaaa ctatcacac gaacatgaa 
        atcaccgaa gatatccgg cgcgaaatg attgacgaa 
        gcctacgag atcatccaa agcagtctt acagttgcg 
        gaaaattcc ccgataatc cacaaatgg gccgcaatc 
        attatcgat cggaaaaac gggatgaaa ggactcgaa 
        catcgagtc aaaaattcg gaagttgtc aaacaccac 
        ctcatcaga acgtgcgaa ctggacccc gacgacgtc 
        accgcgcag tatcttcta ggtcggtgg tgctacgaa 
        atgagcaac atcacgtgg tttcaacgc atcattgcg 
        aaactgttg tacggggaa gccccgaga tcgagcttc 
        gaagaagcc aacaagtat ttatcacgt gctgaagac 
        ttgcacccc cgattttac ctacaaaat acgtattta 
        ttgggcaaa acttgccta aaattaggg caatactac 
        cgggccaaa cactatttc ggagtcgtg ggtaattta 
        cccgtccat aacgagtac gagaggcat tgtgcgaac 
        gacgcgaaa aaattactc aaacgttta gacaagtac 
        agtttggag aaaaacgcc cttttttac gaatacccc 
        ttcggaacc aacgagtaa attttgtgt tctttttgg 
        actcaccta gcatcagag gaacggcct gaaaatcgg 
        gatcgtcct cgtcgaaga ggatgtctt cctcaaact 
        cggtgaatc cctcaagtt tcgtgttac tttcaactc 
        gcaacatct gaaatttga ggaaataaa ggtcatcat 
        cttgaactt gaacgctca ttccattat tttgggcta 
        gcgactgtt gccatggat cttgcccca aacacaaac 
        acttttaac cccaaaaaa acaataaca aaatggcga 
        aacttcgtg gcatatgtc gctaactaa aatagcttt 
        ctttttcca gttattagt cgcattccg gacaagcct 
        cgacctaac ctcaaaacc taacggtac tcgtgtcgt 
        tttttgcgt gattgatgt ttttcccat tgatttatt 
        gcggaaaat taccttttt tggggtcga agtgtgcga 
        attttggcg ttttctttg tgttgcact cacacacag 
        acacgcgac cagtcgact agatgcacc agcgactgg 
        aatgaaatg ccattcagg tcgaagact tttaaaatc 
        gaacggtgg tattatcga aagtcgcgg gattacgag 
        ataacgtag actgaggac ggacgggaa gaaaatcgc 
        cgattttta aactaggtt gaaaaagta aaacggttt 
        ttgtttttg cgaaacaac tgctgcagc tgcaacttg 
        atgaaatgg tcaacaact ggcgtttcg acatattat 
        atttgtttt gtcattgcg attaataaa cggatcggg 
        tgtttttat tattacaca tagtggggc tatttttgg 
        acgttgtga ttggaaaaa attgacttt ttgagataa 
        ggaggccaa attttgatt acaatttcg atttcttaa 
        cgtagcaaa atttagcga aatgctggc aaaacggcg 
        attttgtgg aacatccgt aggtcaagg ttgaaatat 
        accttgtca gtgactgtt tttgacaat tttattgtt 
        ttatgactt tcaattttt ttttgtttg tcgccgtta 
        aattttacc atttccttg ttcattaaa gtataaacg 
        taatgaatg tggggtaaa aaaatagtt atacgttgc 
        cagaaacta taaataccc actgtaatt aatgttaat 
        gataaagaa ggcgctgct ttctaattt ttgccaaat 
        acgagtaca tatactttg gaaatggct gtatttctc 
        actacatct tttttaatt gtgaattgt tggttgttt 
        gatacattt cttaacttt aaacacttt ttgatagct 
        aatctttca aggtcattg tcagaaaca tgacaaagt 
        gcggtcaag tttaattat ttatttctg tcaaattct 
        gattttata aaatgatat tattcaaga ccacttctg 
        acgtttgta tttagtctt ttttaggaa aaatagttg 
        ccaaaataa tgattagaa aatcgtctg cttttgttt 
        atttttaaa ttatttcgt tttaatttt cccttaatt 
        tttttacgc cgttacaaa atcgtagat cataattgt 
        caaaaattg ccgcagtct ttgattttt tactaaata 
        atttttgac ttt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY