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Gene information
General information
TC005863 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6252942..6261152
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005863
      iBeetle screen iB_04024

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005863 RA
        MKEKGNLPK PSAESSKDK SYVQTTQEE KQEQKNSIR 
        VKINLKDCF NQTPKMRPP NECDHSNDL PSRKHKLDP 
        ENKKKHSKK HYKIRICKG VINYMNDQY AAKTTYPRR 
        RRPKIRFYP PSCLNRAVV RRVQCRNDC TTIKTAITI 
        KEEVIDLTS DESTCFMQV KFGVQNEFN FSEIECYLF 
        EPSEVVPSF GAILSELKQ AQVYIPDVR FDTTFNFID 
        LTCDSEEIE DLAISSEKE CANRILHII YQYHKLHHV 
        QLSVENTLS QLQHPCPDD NLTFQFLSQ RASLLGCSL 
        AQILSKTYV RNFDIQRFV DILVHKFEE TYQRSIENE 
        LVLSYLCTM LGTSQAFCA GGTGMKILN LLQVRTKEE 
        PVKGEEEEC QVVFTTEQL DQLQLQIFK YHEIVARQE 
        LLEGGDPFE RCESKVML
      • >TC005863 RB
        MSKVTDPLL LDIEKFMKE KGNLPKPSA ESSKDKSYV 
        QTTQEEKQE QKNSIRVKI NLKDCFNQT PKMRPPNEC 
        DHSNDLPSR KHKLDPENK KKHSKKHYK IRICKGVIN 
        YMNDQYAAK TTYPRRRRP KIRFYPPSC LNRAVVRRV 
        QCRNDCTTI KTAITIKEE VIDLTSDES TCFMQVKFG 
        VQNEFNFSE IECYLFEPS EVVPSFGAI LSELKQAQV 
        YIPDVRFDT TFNFIDLTC DSEEIEDLA ISSEKECAN 
        RILHIIYQY HKLHHVQLS VENTLSQLQ HPCPDDNLT 
        FQFLSQRAS LLGCSLAQI LSKTYVRNF DIQRFVDIL 
        VHKFEETYQ RSIENELVL SYLCTMLGT SQAFCAGGT 
        GMKILNLLQ VRTKEEPVK GEEEECQVV FTTEQLDQL 
        QLQIFKYHE IVARQELLE GGDPFERCE SKVML
      CDS sequence
      • >TC005863 RA
        atgaaggag aaggggaat ctacccaaa ccttctgct 
        gaaagtagc aaagataaa agctacgtg cagacgacc 
        caagaagaa aaacaagaa cagaaaaac tccataaga 
        gtaaaaata aacctgaaa gattgtttc aatcaaact 
        cctaaaatg cgcccccct aacgagtgt gatcatagt 
        aacgatcta ccttcaaga aaacataaa ctagacccg 
        gaaaacaag aaaaagcat tccaagaaa cattacaaa 
        attagaatt tgtaaaggg gtcatcaat tacatgaac 
        gaccaatat gcggccaaa accacgtat ccgagaaga 
        cgccgcccc aaaattcgc ttctacccg cccagctgt 
        ctgaaccgg gctgtggtc cgtagagtg cagtgccgg 
        aacgactgc actactatt aaaactgcc attactata 
        aaagaggaa gtcattgat ctaacttca gacgaaagc 
        acctgtttc atgcaagtg aaatttggc gtccaaaac 
        gaattcaat ttttctgaa atagagtgc tatttgttt 
        gaacctagc gaggttgtg ccctcattt ggggcgatt 
        ctgagcgag ttaaagcag gcacaagtc tatattccg 
        gatgtgcgt tttgacaca acatttaat ttcattgac 
        ttgacttgc gacagtgaa gagattgaa gatttggca 
        atttcgagt gaaaaagaa tgcgccaat cgcatcctg 
        cacataatc taccagtac cacaagttg caccacgtg 
        cagctttcc gtggaaaac actctcagc cagttgcaa 
        cacccgtgt cctgacgat aatctaacg tttcagttc 
        ctgtcacag agagcgtcg ctcctcggc tgcagcctc 
        gcgcaaatt ctctcgaaa acttacgtt aggaatttc 
        gatattcag cgattcgtg gatattttg gtgcacaag 
        tttgaagaa acgtaccag aggagtatc gaaaacgaa 
        ttggttttg tcgtatttg tgcacaatg ctcggcaca 
        agtcaagcg ttctgtgcc ggaggcact ggcatgaaa 
        attctaaac ttgttgcaa gttcgtact aaagaggag 
        ccagtgaag ggggaggag gaagagtgt caagttgtt 
        ttcaccaca gaacagctc gatcagttg cagttgcaa 
        atttttaag taccacgag attgtggca cggcaggaa 
        ctgctagag ggaggggat ccctttgag cgttgtgaa 
        agtaaagtc atgttgtag
      • >TC005863 RB
        atgtcaaaa gtaactgat ccccttctt cttgatatc 
        gaaaaattt atgaaggag aaggggaat ctacccaaa 
        ccttctgct gaaagtagc aaagataaa agctacgtg 
        cagacgacc caagaagaa aaacaagaa cagaaaaac 
        tccataaga gtaaaaata aacctgaaa gattgtttc 
        aatcaaact cctaaaatg cgcccccct aacgagtgt 
        gatcatagt aacgatcta ccttcaaga aaacataaa 
        ctagacccg gaaaacaag aaaaagcat tccaagaaa 
        cattacaaa attagaatt tgtaaaggg gtcatcaat 
        tacatgaac gaccaatat gcggccaaa accacgtat 
        ccgagaaga cgccgcccc aaaattcgc ttctacccg 
        cccagctgt ctgaaccgg gctgtggtc cgtagagtg 
        cagtgccgg aacgactgc actactatt aaaactgcc 
        attactata aaagaggaa gtcattgat ctaacttca 
        gacgaaagc acctgtttc atgcaagtg aaatttggc 
        gtccaaaac gaattcaat ttttctgaa atagagtgc 
        tatttgttt gaacctagc gaggttgtg ccctcattt 
        ggggcgatt ctgagcgag ttaaagcag gcacaagtc 
        tatattccg gatgtgcgt tttgacaca acatttaat 
        ttcattgac ttgacttgc gacagtgaa gagattgaa 
        gatttggca atttcgagt gaaaaagaa tgcgccaat 
        cgcatcctg cacataatc taccagtac cacaagttg 
        caccacgtg cagctttcc gtggaaaac actctcagc 
        cagttgcaa cacccgtgt cctgacgat aatctaacg 
        tttcagttc ctgtcacag agagcgtcg ctcctcggc 
        tgcagcctc gcgcaaatt ctctcgaaa acttacgtt 
        aggaatttc gatattcag cgattcgtg gatattttg 
        gtgcacaag tttgaagaa acgtaccag aggagtatc 
        gaaaacgaa ttggttttg tcgtatttg tgcacaatg 
        ctcggcaca agtcaagcg ttctgtgcc ggaggcact 
        ggcatgaaa attctaaac ttgttgcaa gttcgtact 
        aaagaggag ccagtgaag ggggaggag gaagagtgt 
        caagttgtt ttcaccaca gaacagctc gatcagttg 
        cagttgcaa atttttaag taccacgag attgtggca 
        cggcaggaa ctgctagag ggaggggat ccctttgag 
        cgttgtgaa agtaaagtc atgttgtag
      mRNA sequence
      • >TC005863 RA
        agttaagaa catttcata aaaacgtgt aaagtgtgt 
        tgcctaaca cccaaaact caaccggta gaaagggtt 
        taacagttc tcttcacta gtaacaaag tgttttaaa 
        gcaaaagtt agcttcaag tacttggta ataattgta 
        caattgctt gaaaattgt cgtaattta gccaagatt 
        cgtatagtg gtgtaaata agtaatggt ggtgggagg 
        tgttatgtt acttaacaa gggcggaaa gtaacctgt 
        ttcagtgtc tcaaagttt gaaacttat actttaaaa 
        gtaactgat ccccttctt cttgatatc gaaaaattt 
        atgaaggag aaggggaat ctacccaaa ccttctgct 
        gaaagtagc aaagataaa agctacgtg cagacgacc 
        caagaagaa aaacaagaa cagaaaaac tccataaga 
        gtaaaaata aacctgaaa gattgtttc aatcaaact 
        cctaaaatg cgcccccct aacgagtgt gatcatagt 
        aacgatcta ccttcaaga aaacataaa ctagacccg 
        gaaaacaag aaaaagcat tccaagaaa cattacaaa 
        attagaatt tgtaaaggg gtcatcaat tacatgaac 
        gaccaatat gcggccaaa accacgtat ccgagaaga 
        cgccgcccc aaaattcgc ttctacccg cccagctgt 
        ctgaaccgg gctgtggtc cgtagagtg cagtgccgg 
        aacgactgc actactatt aaaactgcc attactata 
        aaagaggaa gtcattgat ctaacttca gacgaaagc 
        acctgtttc atgcaagtg aaatttggc gtccaaaac 
        gaattcaat ttttctgaa atagagtgc tatttgttt 
        gaacctagc gaggttgtg ccctcattt ggggcgatt 
        ctgagcgag ttaaagcag gcacaagtc tatattccg 
        gatgtgcgt tttgacaca acatttaat ttcattgac 
        ttgacttgc gacagtgaa gagattgaa gatttggca 
        atttcgagt gaaaaagaa tgcgccaat cgcatcctg 
        cacataatc taccagtac cacaagttg caccacgtg 
        cagctttcc gtggaaaac actctcagc cagttgcaa 
        cacccgtgt cctgacgat aatctaacg tttcagttc 
        ctgtcacag agagcgtcg ctcctcggc tgcagcctc 
        gcgcaaatt ctctcgaaa acttacgtt aggaatttc 
        gatattcag cgattcgtg gatattttg gtgcacaag 
        tttgaagaa acgtaccag aggagtatc gaaaacgaa 
        ttggttttg tcgtatttg tgcacaatg ctcggcaca 
        agtcaagcg ttctgtgcc ggaggcact ggcatgaaa 
        attctaaac ttgttgcaa gttcgtact aaagaggag 
        ccagtgaag ggggaggag gaagagtgt caagttgtt 
        ttcaccaca gaacagctc gatcagttg cagttgcaa 
        atttttaag taccacgag attgtggca cggcaggaa 
        ctgctagag ggaggggat ccctttgag cgttgtgaa 
        agtaaagtc atgttgtag ttatttcag atttttcaa 
        agtttaagt gggtttttt tttctaata aaggccgtt 
        tgtgtta
      • >TC005863 RB
        agttaagaa catttcata aaaacgtgt aaagtgtgt 
        tgcctaaca cccaaaact caaccggta gaaagggtt 
        taacagttc tcttcacta gtaacaaag tgttttaaa 
        gcaaaagtt agcttcaag tacttggta ataattgta 
        caattgctt gaaaattgt cgtaattta gccaagatt 
        cgtatagtg gtgtaaata agtaatggt ggtgggagg 
        tgttatgtt acttaacaa gggcggaaa gtaacctgt 
        ttcagtgtc tcaaagttt gaaacttat actttgtct 
        tgcggatca ttttcggtc gtcttccag cttcctgat 
        cattagtaa ataatgtca aaagtaact gatcccctt 
        cttcttgat atcgaaaaa tttatgaag gagaagggg 
        aatctaccc aaaccttct gctgaaagt agcaaagat 
        aaaagctac gtgcagacg acccaagaa gaaaaacaa 
        gaacagaaa aactccata agagtaaaa ataaacctg 
        aaagattgt ttcaatcaa actcctaaa atgcgcccc 
        cctaacgag tgtgatcat agtaacgat ctaccttca 
        agaaaacat aaactagac ccggaaaac aagaaaaag 
        cattccaag aaacattac aaaattaga atttgtaaa 
        ggggtcatc aattacatg aacgaccaa tatgcggcc 
        aaaaccacg tatccgaga agacgccgc cccaaaatt 
        cgcttctac ccgcccagc tgtctgaac cgggctgtg 
        gtccgtaga gtgcagtgc cggaacgac tgcactact 
        attaaaact gccattact ataaaagag gaagtcatt 
        gatctaact tcagacgaa agcacctgt ttcatgcaa 
        gtgaaattt ggcgtccaa aacgaattc aatttttct 
        gaaatagag tgctatttg tttgaacct agcgaggtt 
        gtgccctca tttggggcg attctgagc gagttaaag 
        caggcacaa gtctatatt ccggatgtg cgttttgac 
        acaacattt aatttcatt gacttgact tgcgacagt 
        gaagagatt gaagatttg gcaatttcg agtgaaaaa 
        gaatgcgcc aatcgcatc ctgcacata atctaccag 
        taccacaag ttgcaccac gtgcagctt tccgtggaa 
        aacactctc agccagttg caacacccg tgtcctgac 
        gataatcta acgtttcag ttcctgtca cagagagcg 
        tcgctcctc ggctgcagc ctcgcgcaa attctctcg 
        aaaacttac gttaggaat ttcgatatt cagcgattc 
        gtggatatt ttggtgcac aagtttgaa gaaacgtac 
        cagaggagt atcgaaaac gaattggtt ttgtcgtat 
        ttgtgcaca atgctcggc acaagtcaa gcgttctgt 
        gccggaggc actggcatg aaaattcta aacttgttg 
        caagttcgt actaaagag gagccagtg aagggggag 
        gaggaagag tgtcaagtt gttttcacc acagaacag 
        ctcgatcag ttgcagttg caaattttt aagtaccac 
        gagattgtg gcacggcag gaactgcta gagggaggg 
        gatcccttt gagcgttgt gaaagtaaa gtcatgttg 
        tagttattt cagattttt caaagttta agtgggttt 
        ttttttcta ataaaggcc gtttgtgtt a
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY