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Gene information
General information
TC034026
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005851
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid53: 561194..569160
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034026
    iBeetle screen iB_04023
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034026 RA
      MATTEQFSL RWNNFHSNL TAGFHELLE SSEMVDVTL 
      AVEGHFFQA HKVVLSICS PYFKQMFKV NPCKHPIVI 
      LKDVAHDNM KDILEFMYM GEVNVLREN LATFLRTAE 
      LLQVKGLTG DDSSETSSR KDDKSESIA DNEDDPDLS 
      QFNHLIDSD VELPPYTTP RVPTPPPLN VKRQAKSSI 
      QNSSSKRAK SESLPNKPI KVETEVNTP QNDENDFSE 
      SNPTLKPEK SVTELDEAK DNLKQILEN NFTSAANTD 
      ANLSDQGKP ETKCINRHS YQCEKCYKK FSRRDHLRT 
      HEKNIHGED AGPFVCVIC SQLYKNTES LRKHIAKFH 
      FPKMSEVNQ KVEVKA
    CDS sequence
    • >TC034026 RA
      atggccacg actgagcag ttttcgtta cgttggaac 
      aatttccat tctaacctc actgcgggc ttccatgag 
      ctgctcgaa tcctcggaa atggtcgat gtcacgttg 
      gctgtggaa gggcacttt tttcaggcc cataaagtc 
      gtgctttcg atatgtagc ccctatttt aagcaaatg 
      tttaaagtc aatccctgc aaacacccc atcgttatc 
      ctcaaggac gtggctcat gacaacatg aaggacatc 
      ctggagttc atgtacatg ggggaggtg aacgtcctg 
      agggagaat ctcgccacg tttttgcgc acggcggag 
      ctgctccag gtcaaggga ttgacggga gatgactcg 
      agtgagact tcgtctcgc aaagacgac aaatcggaa 
      tcaatcgcc gataacgaa gatgacccc gacctctcc 
      caatttaat catttaatc gactcagat gtggagtta 
      ccgccttat acaacccct agagttcct acaccgccg 
      cccttaaat gtgaaacgt caagccaaa tcgtcaatc 
      caaaactca agtagcaaa cgagccaaa tccgagtca 
      ttgcccaat aagccgata aaagtggaa actgaagtc 
      aatacgcct caaaatgac gaaaacgat ttttccgaa 
      agtaatcct actctcaag ccggaaaaa agtgtaacc 
      gaactggac gaagccaaa gataacttg aaacagatc 
      ttagaaaat aattttaca agtgctgca aacaccgat 
      gcaaattta agcgatcaa gggaaaccg gaaacgaaa 
      tgcatcaac agacattcg tatcaatgc gagaaatgc 
      tacaaaaaa ttctcgagg agagatcat ctacgaacg 
      cacgagaaa aacatacac ggagaggac gccggaccg 
      tttgtttgt gtcatttgt tcgcaattg tataaaaac 
      acggaaagt ttgcgaaag cacatcgcc aagtttcat 
      tttccgaaa atgagcgaa gttaatcag aaggttgag 
      gtgaaggct tga
    mRNA sequence
    • >TC034026 RA
      agtgtcacc agtcaatca cgcatcccg gatgcccgg 
      attggtccc ggaaatgaa taattaggg ttgcactcg 
      tgttttccc aagtgtttt gccccgaaa atcgctccg 
      tgttatgtt tttcggcct tttgtgcgc gttttttga 
      gcaatagtc agtttttcg cgtgtttac attgtgata 
      attccgagg caaaaggca aaatggcca cgactgagc 
      agttttcgt tacgttgga acaatttcc attctaacc 
      tcactgcgg gcttccatg agctgctcg aatcctcgg 
      aaatggtcg atgtcacgt tggctgtgg aagggcact 
      tttttcagg cccataaag tcgtgcttt cgatatgta 
      gcccctatt ttaagcaaa tgtttaaag tcaatccct 
      gcaaacacc ccatcgtta tcctcaagg acgtggctc 
      atgacaaca tgaaggaca tcctggagt tcatgtaca 
      tgggggagg tgaacgtcc tgagggaga atctcgcca 
      cgtttttgc gcacggcgg agctgctcc aggtcaagg 
      gattgacgg gagatgact cgagtgaga cttcgtctc 
      gcaaagacg acaaatcgg aatcaatcg ccgataacg 
      aagatgacc ccgacctct cccaattta atcatttaa 
      tcgactcag atgtggagt taccgcctt atacaaccc 
      ctagagttc ctacaccgc cgcccttaa atgtgaaac 
      gtcaagcca aatcgtcaa tccaaaact caagtagca 
      aacgagcca aatccgagt cattgccca ataagccga 
      taaaagtgg aaactgaag tcaatacgc ctcaaaatg 
      acgaaaacg atttttccg aaagtaatc ctactctca 
      agccggaaa aaagtgtaa ccgaactgg acgaagcca 
      aagataact tgaaacaga tcttagaaa ataatttta 
      caagtgctg caaacaccg atgcaaatt taagcgatc 
      aagggaaac cggaaacga aatgcatca acagacatt 
      cgtatcaat gcgagaaat gctacaaaa aattctcga 
      ggagagatc atctacgaa cgcacgaga aaaacatac 
      acggagagg acgccggac cgtttgttt gtgtcattt 
      gttcgcaat tgtataaaa acacggaaa gtttgcgaa 
      agcacatcg ccaagtttc attttccga aaatgagcg 
      aagttaatc agaaggttg aggtgaagg cttgatttt 
      tttggtgta aattgttta ggttattgg atagtgccg 
      tcacttgtt tttgaagtg ttaggttta gaatttgta 
      tttgtaaat ttgattatt taaccagtt tcgacctaa 
      gaacatgac attttcttt tattttttg ctagtcagg 
      ttgtattct gtatttttc aactaccag aaaagactt 
      tatttaatt cttactgta gtgacacaa aacatagat 
      atttaatta ttttctatt gttattata taactgtta 
      tggtgattt atattaaat ttcgtttta tgttattat 
      agtgtaagt gccttatgt atttcatgt cgtaataaa 
      ttggttatc caaag