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Gene information
General information
TC005842 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6758319..6760210
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005842
    iBeetle screen iB_04020

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005842 RA
      MPPVVESRP TWSSNFRLY LAMFSYSTG ITIPKFASP 
      WLLKTNVGI LLLIPVQLL VMFLTTIPC MFMELALGQ 
      YSNRAVIRV WDLCPLLRG IGLCSFLTF LLYQSYYNF 
      LCTCSLCFT FLSFTSEPQ WRSCNRSWN KINCFTLRD 
      NYTKRLECS QRYGREKCD KMQWQSSVE QFYFNRVLN 
      TDETSGYVG APQPELLVY GLVTIILIF FANYGGPKT 
      TEKFLLVLA TVPTVQMVV LFFAPLSDQ NGFVTLNYL 
      FTQLDVKNL SNFKTWTRI INLSLSSPG LGFGAFIAF 
      GTQSTFRTP LHFKAIIIS VTSVLFTVL YSIIVQNFL 
      ISLCIQSQM LFDDFFKLE QELEMPEAV FFLEHSSRF 
      WITMWLSNC YMTGLRSLI VMMCLNTEV VYNSLPKAR 
      KRHTLCVLT FSLIIYTGT IFLSTRIGY SVFKTIESV 
      LNDFCVPFL NTLQLVGVV YFYGITKFR DDVHFMLGI 
      RSSVYWNTL YVLNPLLLG IVLAYNLHD FFTGGSFNF 
      HSLGIFRIS LIWFSLVVV TLYAFFYII VSFFGHHFK 
      VCKSSPEWG PRHGSLFVS RKMFKAYNV AKEYLYRQE 
      RFSQQ
    CDS sequence
    • >TC005842 RA
      atgcctccg gtggtggaa tcccgccca acgtggagc 
      tccaatttc cggctctac ctggcaatg ttcagctac 
      agtaccggg ataacgatc ccgaagttc gctagtcct 
      tggctcctc aagaccaat gtcgggatc ctcctcctg 
      atccccgtc cagctcctg gtcatgttc ctgacgacg 
      atcccttgc atgttcatg gagctagcc ctggggcag 
      tactccaac cgggcggtg atacgagtg tgggacttg 
      tgcccgtta ctgcgagga atcggctta tgcagtttc 
      ctcacgttt ctgctctac caatcgtat tacaatttc 
      ctctgtaca tgtagtctt tgtttcacg tttttgtca 
      ttcacgagc gaaccacaa tggagatct tgtaacagg 
      agttggaat aagataaat tgcttcact ttgcgagac 
      aattacacg aagcgactc gaatgcagc cagagatac 
      ggcagggaa aagtgcgac aagatgcag tggcagtcg 
      agcgtggag cagttttac ttcaaccgg gtgctgaac 
      acggatgag acttccggt tatgtgggg gccccccag 
      cctgaacta ctggtctat ggattggtc acaattatt 
      ttgattttt tttgcgaac tatggtggc cccaaaacc 
      accgaaaaa ttccttctg gtgttggca acggtgccc 
      acggttcaa atggttgtc ttgtttttt gcccccttg 
      tcggaccag aacggcttt gtgaccctt aattacttg 
      ttcacccaa ctggatgtg aaaaatttg tccaatttt 
      aagacctgg actaggatt ataaacttg tctctgagt 
      agtccaggc ctggggttt ggtgccttc attgctttc 
      ggcacgcag agcactttc aggacccct ctgcacttc 
      aaggcgatc ataataagc gtcacttcg gttttattc 
      acagtcttg tacagtatc atagtccag aacttttta 
      atttcgttg tgcatccaa agccagatg ttgtttgac 
      gattttttt aaactggaa caggagctg gagatgcct 
      gaggccgtg tttttcctg gaacacagt tccaggttt 
      tggataacc atgtggtta tccaattgt tacatgacc 
      ggtctccga agtttgatt gtgatgatg tgcctgaac 
      accgaagtg gtttataac tcgttacca aaggccaga 
      aaacgacac acactctgt gttctaacg ttctctctt 
      ataatctac accgggacg attttcctg tcaacgaga 
      attggttac tccgtgttt aaaaccatc gaaagtgtg 
      ttaaacgac ttttgtgtt ccgttcctc aacacttta 
      caactcgtc ggagttgtg tatttttac ggtattact 
      aaatttaga gacgatgtc cactttatg ttagggata 
      cggtcgtca gtgtactgg aacacgctc tacgtcctt 
      aatcccctt cttctgggg attgtgttg gcctacaac 
      ttgcacgat ttcttcact ggggggagt ttcaatttt 
      cacagtctt ggtattttt aggataagt cttatttgg 
      tttagtctt gttgttgtc acgttgtat gcttttttc 
      tacattatt gttagtttc tttgggcac cattttaaa 
      gtttgtaag tcgtcacct gagtggggc cctcgacac 
      ggctccctg tttgtgagc cgaaaaatg ttcaaggcc 
      tataacgtg gccaaggag tatttgtat cgtcaagaa 
      cggttcagt cagcaataa
    mRNA sequence
    • >TC005842 RA
      aaagttgac gcgcgcgtt tcaaatcca tgcctccgg 
      tggtggaat cccgcccaa cgtggagct ccaatttcc 
      ggctctacc tggcaatgt tcagctaca gtaccggga 
      taacgatcc cgaagttcg ctagtcctt ggctcctca 
      agaccaatg tcgggatcc tcctcctga tccccgtcc 
      agctcctgg tcatgttcc tgacgacga tcccttgca 
      tgttcatgg agctagccc tggggcagt actccaacc 
      gggcggtga tacgagtgt gggacttgt gcccgttac 
      tgcgaggaa tcggcttat gcagtttcc tcacgtttc 
      tgctctacc aatcgtatt acaatttcc tctgtacat 
      gtagtcttt gtttcacgt ttttgtcat tcacgagcg 
      aaccacaat ggagatctt gtaacagga gttggaata 
      agataaatt gcttcactt tgcgagaca attacacga 
      agcgactcg aatgcagcc agagatacg gcagggaaa 
      agtgcgaca agatgcagt ggcagtcga gcgtggagc 
      agttttact tcaaccggg tgctgaaca cggatgaga 
      cttccggtt atgtggggg ccccccagc ctgaactac 
      tggtctatg gattggtca caattattt tgatttttt 
      ttgcgaact atggtggcc ccaaaacca ccgaaaaat 
      tccttctgg tgttggcaa cggtgccca cggttcaaa 
      tggttgtct tgttttttg cccccttgt cggaccaga 
      acggctttg tgaccctta attacttgt tcacccaac 
      tggatgtga aaaatttgt ccaatttta agacctgga 
      ctaggatta taaacttgt ctctgagta gtccaggcc 
      tggggtttg gtgccttca ttgctttcg gcacgcaga 
      gcactttca ggacccctc tgcacttca aggcgatca 
      taataagcg tcacttcgg ttttattca cagtcttgt 
      acagtatca tagtccaga actttttaa tttcgttgt 
      gcatccaaa gccagatgt tgtttgacg attttttta 
      aactggaac aggagctgg agatgcctg aggccgtgt 
      ttttcctgg aacacagtt ccaggtttt ggataacca 
      tgtggttat ccaattgtt acatgaccg gtctccgaa 
      gtttgattg tgatgatgt gcctgaaca ccgaagtgg 
      tttataact cgttaccaa aggccagaa aacgacaca 
      cactctgtg ttctaacgt tctctctta taatctaca 
      ccgggacga ttttcctgt caacgagaa ttggttact 
      ccgtgttta aaaccatcg aaagtgtgt taaacgact 
      tttgtgttc cgttcctca acactttac aactcgtcg 
      gagttgtgt atttttacg gtattacta aatttagag 
      acgatgtcc actttatgt tagggatac ggtcgtcag 
      tgtactgga acacgctct acgtcctta atccccttc 
      ttctgggga ttgtgttgg cctacaact tgcacgatt 
      tcttcactg gggggagtt tcaattttc acagtcttg 
      gtattttta ggataagtc ttatttggt ttagtcttg 
      ttgttgtca cgttgtatg cttttttct acattattg 
      ttagtttct ttgggcacc attttaaag tttgtaagt 
      cgtcacctg agtggggcc ctcgacacg gctccctgt 
      ttgtgagcc gaaaaatgt tcaaggcct ataacgtgg 
      ccaaggagt atttgtatc gtcaagaac ggttcagtc 
      agcaataac ttgtgcaat aaaatattt caaataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY