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Gene information
General information
TC005822 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7178586..7185349
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC005822
      iBeetle screen iB_04018

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
      cuticle everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

      gut remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
      larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
      legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC005822 RA
        MSDLVWWTL FATLLLLLI VFTVKLITS IVSDVYCKE 
        ESGSKDFED DFPVEIKKT NEVQNEEDT SPQRYQINP 
        RSLLQNIRE TGHHDLERI RLIEENVRR QEEEMNREE 
        EMYVTIAKT GTKDETKKD EDQENYTEE EKQILEKIE 
        DIKSSVQSN AEQIPLCFD RNQLKFYDI NQNLLMSAF 
        SLNEIKCEE NDKLKRRKD LVSIYIREC QSKLEKKAG 
        ENSFIAEET DGQVENEAK ESPLSAQEQ EIWNQIEKI 
        RTKIEAYAH QIPNCLDGR NHLKYYEIN QNLLLCSIN 
        LGEIDCQKN DILIKKKQD VSTYIRQCQ RQLNKKSER 
        NTM
      CDS sequence
      • >TC005822 RA
        atgtcggat ttggtgtgg tggacactt tttgcaacc 
        ttattacta ctcttgatc gtgtttact gtgaaacta 
        ataaccagt attgtgtcc gatgtttat tgcaaggaa 
        gaaagtggt tccaaagat tttgaagat gactttcca 
        gttgaaata aagaaaacc aacgaagta caaaatgaa 
        gaagacacg tccccacag aggtaccaa attaatccc 
        agatcgctg ttgcaaaac atcagggag acaggacac 
        cacgatttg gaacgaata agactaatt gaagaaaac 
        gtaagacga caagaagaa gaaatgaac cgggaagaa 
        gaaatgtac gtcacgata gcaaaaaca ggcaccaaa 
        gacgaaaca aagaaagac gaagaccag gaaaattac 
        accgaggaa gagaaacaa attctggag aagattgaa 
        gatatcaaa agttcagtc cagagcaac gctgagcag 
        attcccctg tgtttcgac cgaaaccag ttgaaattc 
        tacgacatc aaccaaaac ctcctaatg agcgccttc 
        agtctgaac gagatcaaa tgcgaagaa aacgacaag 
        ttgaagcga agaaaggac ttggtctcg atctacatc 
        cgagaatgc caaagcaaa ctggagaag aaagccggc 
        gaaaactcg ttcatagcc gaggaaacg gacgggcaa 
        gtcgaaaat gaagcgaaa gagagtccg ctaagtgcc 
        caagaacag gaaatttgg aaccaaatc gaaaaaatc 
        cgaaccaaa atcgaggcc tacgcccac caaatcccc 
        aactgtttg gacggcaga aaccacctc aaatattac 
        gaaatcaat cagaacttg ttgttgtgt tcgataaac 
        ttgggcgaa atcgactgc caaaaaaac gacattttg 
        attaagaaa aaacaggac gtgtcgacc tacattcgg 
        cagtgtcag agacaactc aacaaaaaa tcggaacga 
        aatactatg taa
      mRNA sequence
      • >TC005822 RA
        gatatcagt tattataaa agatatgag ggaaatagt 
        aaattgctt acaactgca cgaaaacat gacaaaatc 
        aaactaatc aaaatgtcg gatttggtg tggtggaca 
        ctttttgca accttatta ctactcttg atcgtgttt 
        actgtgaaa ctaataacc agtattgtg tccgatgtt 
        tattgcaag gaagaaagt ggttccaaa gattttgaa 
        gatgacttt ccagttgaa ataaagaaa accaacgaa 
        gtacaaaat gaagaagac acgtcccca cagaggtac 
        caaattaat cccagatcg ctgttgcaa aacatcagg 
        gagacagga caccacgat ttggaacga ataagacta 
        attgaagaa aacgtaaga cgacaagaa gaagaaatg 
        aaccgggaa gaagaaatg tacgtcacg atagcaaaa 
        acaggcacc aaagacgaa acaaagaaa gacgaagac 
        caggaaaat tacaccgag gaagagaaa caaattctg 
        gagaagatt gaagatatc aaaagttca gtccagagc 
        aacgctgag cagattccc ctgtgtttc gaccgaaac 
        cagttgaaa ttctacgac atcaaccaa aacctccta 
        atgagcgcc ttcagtctg aacgagatc aaatgcgaa 
        gaaaacgac aagttgaag cgaagaaag gacttggtc 
        tcgatctac atccgagaa tgccaaagc aaactggag 
        aagaaagcc ggcgaaaac tcgttcata gccgaggaa 
        acggacggg caagtcgaa aatgaagcg aaagagagt 
        ccgctaagt gcccaagaa caggaaatt tggaaccaa 
        atcgaaaaa atccgaacc aaaatcgag gcctacgcc 
        caccaaatc cccaactgt ttggacggc agaaaccac 
        ctcaaatat tacgaaatc aatcagaac ttgttgttg 
        tgttcgata aacttgggc gaaatcgac tgccaaaaa 
        aacgacatt ttgattaag aaaaaacag gacgtgtcg 
        acctacatt cggcagtgt cagagacaa ctcaacaaa 
        aaatcggaa cgaaatact atgtaattt ataaaaatt 
        ttgtttacg aattttgtt tttcttttt gttccttcc 
        cttgcgcca tctcatggc ggtgtgacg tttcaaatg 
        accggccgt ggagagact ggccttacc gaccggatc 
        gatacgtta cgacaagtg gcgccacac tcgaatcca 
        tcgccgttt tatttattt tagcgagtt attattcga 
        ttgattttc aaggggcgg cattttacg ttaaaggca 
        ttgcctact tattttacc cgttttata ttttaagga 
        aactgaaaa tatcattcg ttcttttca aaatttctt 
        gcgatggaa aataagata aacgcttgc aaaagaaga 
        aaacctgac agatatt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY