Status
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Gene information
General information
TC034050
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005796,TC005797
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7463610..7494193
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034050
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04015

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs podomer shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs pretarsus rounded - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus split - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs duplicated - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion delayed (still pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (41 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 5

    ommatidia potentially structure fused - Number of animals showing the phenotype: 4

    longitudinal dorsal thoracic muscles structure not present - Number of animals showing the phenotype: 5

    oblique dorsal thoracic muscles size shortened - Number of animals showing the phenotype: 5

    Adult morphology
    eyes structure irregular - Number of animals showing the phenotype: 2


    antenna orientation bent - Number of animals showing the phenotype: 5

    ommatidia potentially fused - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034050 RA
      MHLPVSLSS CTVSEEAWP KSSGMCEPS SGYVSNNQD 
      IWWTEHLIH EIQTEHPGE LVRTGSPYF LCSALPTHW 
      RSNKTLPVA FKVVALGDI GDGTIVTVR AGNDENCCA 
      ELRNSTAVM KNQVAKFND LRFVGRSGR GKSFSITIT 
      VSTTPPQVA TYTKAIKVT VDGPREPRS KTSEYLPWI 
      LLHREPWII QPGQHTAYR AIGLGQRPF LDGSFSTHL 
      RDLEAYKKQ RGGPSSQSS DGSQSSYKQ DSQDGSLHP 
      NPPPCPPAT WPDYSYTPY SPQTGYYDH QDSATSLHL 
      PTVLPEPGH NEFINTSLT SPPPMLNSA KSELDPMRY 
      SDNSTYYPN NWTPNSYPN NYNYYNTPN NNQQYPSTM 
      VLYPPLYST QNQIHFHLH GSSEKIDQY LSTESLSLT 
      PTRAIELAQ TVTTEVAQS HETLEDTER THDPASVWR 
      PY
    CDS sequence
    • >TC034050 RA
      atgcatctc ccagtctcc ctctcgtct tgcaccgtt 
      tcggaggaa gcgtggccg aagagcagc ggcatgtgc 
      gagccatcg tcgggctac gtctccaac aaccaggac 
      atctggtgg acggaacac ctcatccac gagatccag 
      acggagcat cccggcgaa ttggtccgc actggaagt 
      ccctatttt ctctgttcg gcccttccg actcattgg 
      aggtcaaac aaaacgctt ccggtggcg ttcaaagtg 
      gtagctttg ggggatatt ggcgatgga acaatagtg 
      actgtgaga gcgggcaat gacgagaac tgttgtgca 
      gaattgagg aattcaaca gcggtgatg aagaaccaa 
      gtggccaag tttaacgat ttgaggttc gtcgggagg 
      agtggacgg ggtaaatcg ttttcgatt acgattacg 
      gtgtcgacg acgccgcca caggtcgcg acctacacc 
      aaagccatc aaagtgacc gtggatggg cccagggaa 
      cccagatcg aagaccagt gagtattta ccttggatt 
      ttgttacac cgcgaaccg tggataatt caacccggc 
      cagcacacc gcctaccgg gccatcggc ctggggcag 
      aggcccttc ctggacggc tccttctcg acgcacctg 
      cgggacctg gaggcgtac aaaaaacag cgcggtgga 
      ccgtcgtcg caaagcagc gacggctcg caaagctcc 
      tacaagcag gactcgcaa gacgggtcg ctgcacccc 
      aacccgccc ccctgtccc cctgccacg tggcccgac 
      tactcgtac accccctac agcccccag acgggctac 
      tacgaccac caggactcc gccacctcg cttcatctt 
      cccaccgtt ctccccgag ccaggccat aacgaattc 
      atcaacact tcgctcacc tcccccccg cctatgctc 
      aacagtgcc aagtccgag ctggacccc atgaggtac 
      tccgataac agtacctac taccccaac aattggacc 
      ccgaactcg taccccaac aactacaac tactacaac 
      acgcccaac aacaaccag cagtacccc agtactatg 
      gtcctctac ccccctttg tactccacc cagaaccag 
      attcacttc cacctgcat ggtagtagc gaaaagatc 
      gaccagtac ttaagtacc gaaagtctg agtttgacc 
      ccaactagg gctatagaa ctagcacag actgtgact 
      acagaagtg gcacaatcg catgaaacg ctcgaggat 
      acggaaagg acgcatgac cccgccagt gtctggagg 
      ccgtactga
    mRNA sequence
    • >TC034050 RA
      tcagtcgcg atttgacaa tcgacggtt gtggtgttg 
      ttcgcggat gcgacgttc acgtgtttc gatgatttt 
      ttcgaaaaa acaagtgca ggttttgag tgtggtggt 
      tttttgagt gagatacag ggattagcg ccccctgcc 
      ccatgcatc tcccagtct ccctctcgt cttgcaccg 
      tttcggagg aagcgtggc cgaagagca gcggcatgt 
      gcgagccat cgtcgggct acgtctcca acaaccagg 
      acatctggt ggacggaac acctcatcc acgagatcc 
      agacggagc atcccggcg aattggtcc gcactggaa 
      gtccctatt ttctctgtt cggcccttc cgactcatt 
      ggaggtcaa acaaaacgc ttccggtgg cgttcaaag 
      tggtagctt tgggggata ttggcgatg gaacaatag 
      tgactgtga gagcgggca atgacgaga actgttgtg 
      cagaattga ggaattcaa cagcggtga tgaagaacc 
      aagtggcca agtttaacg atttgaggt tcgtcggga 
      ggagtggac ggggtaaat cgttttcga ttacgatta 
      cggtgtcga cgacgccgc cacaggtcg cgacctaca 
      ccaaagcca tcaaagtga ccgtggatg ggcccaggg 
      aacccagat cgaagacca gtgagtatt taccttgga 
      ttttgttac accgcgaac cgtggataa ttcaacccg 
      gccagcaca ccgcctacc gggccatcg gcctggggc 
      agaggccct tcctggacg gctccttct cgacgcacc 
      tgcgggacc tggaggcgt acaaaaaac agcgcggtg 
      gaccgtcgt cgcaaagca gcgacggct cgcaaagct 
      cctacaagc aggactcgc aagacgggt cgctgcacc 
      ccaacccgc ccccctgtc cccctgcca cgtggcccg 
      actactcgt acaccccct acagccccc agacgggct 
      actacgacc accaggact ccgccacct cgcttcatc 
      ttcccaccg ttctccccg agccaggcc ataacgaat 
      tcatcaaca cttcgctca cctcccccc cgcctatgc 
      tcaacagtg ccaagtccg agctggacc ccatgaggt 
      actccgata acagtacct actacccca acaattgga 
      ccccgaact cgtacccca acaactaca actactaca 
      acacgccca acaacaacc agcagtacc ccagtacta 
      tggtcctct acccccctt tgtactcca cccagaacc 
      agattcact tccacctgc atggtagta gcgaaaaga 
      tcgaccagt acttaagta ccgaaagtc tgagtttga 
      ccccaacta gggctatag aactagcac agactgtga 
      ctacagaag tggcacaat cgcatgaaa cgctcgagg 
      atacggaaa ggacgcatg accccgcca gtgtctgga 
      ggccgtact gatagccct ccaagggct gttcttatt 
      tattgtaaa atttgtatt ataaaaagt attattaca 
      ggaaactgt aactattat ttttctact cgctttatt 
      agcttaccg actcgtttc ttttgtgtc ttattttag 
      atttatacg atggttgtg tctacgaat gttgcaata 
      acatctcca tgtgacaat ttttctgtc aattatatc 
      gtgtcaatg tcaatacat atcaaaatc tttgtgatt 
      ttaaaaact acgggcgct cgagcccat tttcaaagt 
      atttatatt gtactaata gtagaacaa gttaataaa 
      cgattaatg agtgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY