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Gene information
General information
TC005785 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 610084..615824
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005785
    iBeetle screen iB_04013
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labium not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (22 dpi)
    Days post injection


    Pupal morphology
    labial palp potentially structure not fused - Number of animals showing the phenotype: 8

    Adult morphology
    elytra dorsal orientation not closed - Number of animals showing the phenotype: 2


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005785 RA
      MKLLLTDNT IEALDIPIP SPCTNPAQV HHQRIAHLP 
      FNHRGGPTR PPLSTPLDT EPISSSPSV AVAVTKMNI 
      NPAYDAYQM PKTGFVRQV KQEADSTSP LLTPPHNPA 
      YSPPPSYCD PWYNPYYYQ YLQNAALYH KLQFRGPPR 
      TPLQPKLVA GKLKPKRQF ICKYCNRQF TKSYNLLIH 
      ERTHTDERP YSCDICGKA FRRQDHLRD HRYIHSKEK 
      PFKCTECGK GFCQSRTLA VHKILHMEE SPHKCPVCS 
      RSFNQRSNL KTHLLTHTE RPLECTLCA QFFASYSDL 
      KTHEARHCP KVEEVVQPT TCLDLTKKS ASPKVKLGF 
      SIEDIMKR
    CDS sequence
    • >TC005785 RA
      atgaagctg ttactaact gacaataca atagaagca 
      ttagatatc ccaattcct tcaccctgc accaaccct 
      gcgcaagtc caccaccag cgaatagca cacctccca 
      tttaatcac agaggcggg cccaccaga cccccactt 
      tctaccccg ctggacacc gaaccgatc agttcttca 
      ccgagtgtt gcagtggca gtgacaaaa atgaacatt 
      aatcccgct tacgacgcc taccagatg cccaagacc 
      ggctttgtc cggcaagtc aaacaggag gctgacagt 
      acttcgccc cttttgacg cccccacac aaccccgcc 
      tattcgccg cccccgagc tactgcgat ccgtggtac 
      aacccttat tactaccag tatttgcaa aatgcggcc 
      ctgtatcac aagttgcaa ttcaggggg cccccaagg 
      acccccctt cagcctaaa ttagtggct gggaagctc 
      aaacccaag cgacaattc atctgcaag tattgtaat 
      cgacaattc actaaaagc tacaacttg ttgatccat 
      gagaggaca catacggac gaaaggccc tactcgtgt 
      gacatctgt gggaaggcg ttccggcgt caagaccat 
      ctgagggac cacaggtac atccacagc aaggagaag 
      ccgttcaag tgcacggag tgcggcaag ggcttctgc 
      cagagccgg actttggcg gtgcacaaa atcctccac 
      atggaggaa tccccgcac aagtgcccc gtgtgctcg 
      cgctcgttc aaccagcgc tccaatctc aagacgcac 
      ttgttgacc cacaccgag cgccccctt gagtgcacc 
      ctctgtgcg cagtttttc gccagctac tccgatctc 
      aagacgcac gaggcgcgc cattgcccc aaggtggag 
      gaagtcgtc cagcccacg acgtgcctc gatttgacc 
      aagaaaagt gcaagtccc aaagtcaag ctggggttc 
      agtatcgag gacattatg aagcgataa
    mRNA sequence
    • >TC005785 RA
      cttctagtt ttaattata aaatgaagc tgttactaa 
      ctgacaata caatagaag cattagata tcccaattc 
      cttcaccct gcaccaacc ctgcgcaag tccaccacc 
      agcgaatag cacacctcc catttaatc acagaggcg 
      ggcccacca gacccccac tttctaccc cgctggaca 
      ccgaaccga tcagttctt caccgagtg ttgcagtgg 
      cagtgacaa aaatgaaca ttaatcccg cttacgacg 
      cctaccaga tgcccaaga ccggctttg tccggcaag 
      tcaaacagg aggctgaca gtacttcgc cccttttga 
      cgcccccac acaaccccg cctattcgc cgcccccga 
      gctactgcg atccgtggt acaaccctt attactacc 
      agtatttgc aaaatgcgg ccctgtatc acaagttgc 
      aattcaggg ggcccccaa ggacccccc ttcagccta 
      aattagtgg ctgggaagc tcaaaccca agcgacaat 
      tcatctgca agtattgta atcgacaat tcactaaaa 
      gctacaact tgttgatcc atgagagga cacatacgg 
      acgaaaggc cctactcgt gtgacatct gtgggaagg 
      cgttccggc gtcaagacc atctgaggg accacaggt 
      acatccaca gcaaggaga agccgttca agtgcacgg 
      agtgcggca agggcttct gccagagcc ggactttgg 
      cggtgcaca aaatcctcc acatggagg aatccccgc 
      acaagtgcc ccgtgtgct cgcgctcgt tcaaccagc 
      gctccaatc tcaagacgc acttgttga cccacaccg 
      agcgccccc ttgagtgca ccctctgtg cgcagtttt 
      tcgccagct actccgatc tcaagacgc acgaggcgc 
      gccattgcc ccaaggtgg aggaagtcg tccagccca 
      cgacgtgcc tcgatttga ccaagaaaa gtgcaagtc 
      ccaaagtca agctggggt tcagtatcg aggacatta 
      tgaagcgat aaatggatt gcgcgtttg ttcagccaa 
      agatgacgc tacctctga ttttcgatg agactgagg 
      acgagttac cgtgttgtt gttgtacat agctttgtg 
      taaataaat atttatttc cttg