Status
Please wait ...
Gene information
General information
TC005781 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 724238..727565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005781
    iBeetle screen iB_04011
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    Comment: No phenotype found!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005781 RA
      MAEFLQYDW RPHKIVPEP APRSEPLDK SVSIQALRQ 
      MIQQSTDLE LKNNIPQDD KTLLKFLFA RKFNIPDTY 
      LLVQNYYYY RKKNHIIFE NFTPSAPDI KNALESSLP 
      GVLPSKDRK GRCVLILNA TNWDCNYSL ISIYRAILL 
      SLEHLVNEV HNQARGFVV IVDWTEFSF RQSTNLKPS 
      ILKLMIEGL QDCFPAKFK GIHFIGQPW YVEAALTFI 
      KPFLKEKIK ERIFVHGNN LSTLHDYVH KDILPAELG 
      GEQPSYNPE IWIKNLGEE NGNDAKPKQ GF
    CDS sequence
    • >TC005781 RA
      atggcggag ttcctgcag tatgactgg cgcccccat 
      aaaatcgtc ccggagccg gcgccccgc agtgagccc 
      ctcgacaaa tcggtctca atccaggcc ttaagacag 
      atgatccaa caatcaact gacttggaa cttaaaaat 
      aacattccc caagacgat aaaaccctc ctcaagttc 
      ctctttgcc cggaaattc aacattcca gacacctat 
      cttctggtc cagaattat tactactac cggaagaaa 
      aaccacata attttcgaa aatttcact ccaagcgcc 
      cccgacatt aaaaacgcc ctggaaagc agtctccca 
      ggcgtttta ccctcgaaa gaccgcaaa ggacgctgc 
      gttttaatc ctgaacgca acaaactgg gattgtaac 
      tactcctta atcagtatt tatcgcgcg attttgcta 
      agtttggag cacttagtg aacgaagtg cacaaccag 
      gccagaggc ttcgtcgtt atagtcgac tggacggaa 
      ttttccttc cgccaaagt acaaatttg aaaccttcg 
      atacttaaa ttaatgatc gagggtttg caggactgt 
      ttccccgcg aaattcaaa gggattcat tttattggc 
      cagccgtgg tacgtcgaa gccgccttg actttcatc 
      aaaccgttt ttgaaggag aaaatcaag gaacggatt 
      ttcgtacat gggaataat ctgagtact ttacacgat 
      tatgtgcac aaggatatt ttaccggct gagttaggc 
      ggggaacaa ccgagttac aatcccgaa atttggatt 
      aaaaatttg ggggaggaa aatggcaac gacgcaaag 
      ccaaagcag ggattttag
    mRNA sequence
    • >TC005781 RA
      aaccaagac gtaatacat aaacccact cctccattc 
      cgacgtcac agcatttga atcagcggt tggttcacc 
      tggttcacc atttaccat taccaagga tgacctggt 
      agaggtgcc tgtctcgcg tgagtcacc atcagtgaa 
      attttattc cactcgagc agaaattta cagtgttta 
      ccacgttta ccaaatatt taaccacaa taatcgtaa 
      atcaacagt tctgcttaa aatgtgagt gcttgcagc 
      gaaatacca gcgacatta tatcacaaa tagaccaat 
      ggcggagtt cctgcagta tgactggcg cccccataa 
      aatcgtccc ggagccggc gccccgcag tgagcccct 
      cgacaaatc ggtctcaat ccaggcctt aagacagat 
      gatccaaca atcaactga cttggaact taaaaataa 
      cattcccca agacgataa aaccctcct caagttcct 
      ctttgcccg gaaattcaa cattccaga cacctatct 
      tctggtcca gaattatta ctactaccg gaagaaaaa 
      ccacataat tttcgaaaa tttcactcc aagcgcccc 
      cgacattaa aaacgccct ggaaagcag tctcccagg 
      cgttttacc ctcgaaaga ccgcaaagg acgctgcgt 
      tttaatcct gaacgcaac aaactggga ttgtaacta 
      ctccttaat cagtattta tcgcgcgat tttgctaag 
      tttggagca cttagtgaa cgaagtgca caaccaggc 
      cagaggctt cgtcgttat agtcgactg gacggaatt 
      ttccttccg ccaaagtac aaatttgaa accttcgat 
      acttaaatt aatgatcga gggtttgca ggactgttt 
      ccccgcgaa attcaaagg gattcattt tattggcca 
      gccgtggta cgtcgaagc cgccttgac tttcatcaa 
      accgttttt gaaggagaa aatcaagga acggatttt 
      cgtacatgg gaataatct gagtacttt acacgatta 
      tgtgcacaa ggatatttt accggctga gttaggcgg 
      ggaacaacc gagttacaa tcccgaaat ttggattaa 
      aaatttggg ggaggaaaa tggcaacga cgcaaagcc 
      aaagcaggg attttagga cgatttggg cattgtagt 
      ggctgcact gcacatgac aataatttg aacaaagtg 
      ctgtgattt atggaattg gcgccaaat attttgtag 
      ttgcaaaat aaatgctga actgactg