Status
Please wait ...
Gene information
General information
TC005738 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8305830..8311009
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005738
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04003

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Fertility
    egg production: number >150% of expected -
    Comment: No phenotype found!!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005738 RA
      MSEDGFSDG FDEPTMEIQ IDTLMGTSF DMRVSATDT 
      VREIKQRIQ RVEGIPIHQ QNLIFQSKE LKDSRRLCD 
      AGIKNGSII KLVIAMKGG PISTRRLSV SCEHHMMLK 
      ELKELLENT RDEIGDKLA PGSKVSVLV FKEGDILNL 
      LRVIENEDG SYSPYSEKP ISPPSKPRK ESQMAMFEK 
      LVEDTDMMT KISNLRKKM EDLSLRRQS RNVKHKDEE 
      KVSYKLLTE CSNDLLDVD LKIFEKNLE EESQSETEE 
      VALKDKHRI KARKSVYSE SKGAFSHTK QNYARIHKK 
      TRELRGEGS SKEGDLCVA VPKNTVHLT RLVLSEGVE 
      RPKSTDSIE LEENAQDLW EIHEPATER LKHSATCKF 
      GLLKRRQSL DYGGFEGLE NAPKQEYNY GEVCSGNFF 
      HVPQFVSSP SAIPPQYAP KEQDLYYFR KNNRSPLYG 
      RKNEESFLE CSSIQRLKN DEKSLSESL LSGHDESID 
      ELYGYYNLG CAIGESKVS KKKEVELPP VIKKKSRCS 
      ECNKRLNIT NIYDCRCGK IFCSQHRYS EVHRCSYDY 
      KTEGRRILE HQNPLVTAD KINRI
    CDS sequence
    • >TC005738 RA
      atgtctgaa gatgggttt tccgacggt ttcgacgaa 
      cccaccatg gaaatacag atcgacacg ctcatgggc 
      acctcgttc gacatgcgg gtctcggcg accgacacc 
      gtcagggag atcaagcag agaatccag cgagtcgaa 
      ggtatcccg atccaccaa caaaacctc atattccaa 
      tccaaggaa ttgaaagac tctcgtcga ttatgtgat 
      gcaggcatc aaaaacggt tcgataata aaactagtg 
      atagcgatg aaaggcggt ccgatatca acccgtcgc 
      ttatcagtt agttgcgaa caccacatg atgttaaaa 
      gaactgaaa gaactcttg gaaaacacc agggatgaa 
      atcggcgat aaactcgcc cccggctca aaagtatca 
      gttttagtg ttcaaagaa ggcgacatt ttaaattta 
      cttcgagta attgaaaac gaagacggt tcatattcc 
      ccttacagc gagaagcca atatcgccc ccgagcaag 
      ccacggaag gagtcccaa atggcaatg ttcgaaaaa 
      ctggtcgaa gacaccgat atgatgacg aaaatctcg 
      aatttgaga aagaaaatg gaagatttg agtcttcga 
      cgacagtcg cgcaacgtt aaacacaaa gacgaggaa 
      aaagtctcc tacaagtta ctgaccgag tgttcgaac 
      gatttactc gacgtggac ttgaagatt tttgagaag 
      aatctcgaa gaagagagc cagtccgag accgaggaa 
      gtggcccta aaagacaag caccggatc aaggcccgg 
      aagtcggtc tacagcgaa agcaagggg gccttctcg 
      cacacgaag caaaactac gcccgcatc cacaagaag 
      acgcgcgaa ctgcggggc gaaggctcg agcaaagag 
      ggcgacctg tgcgtcgcc gtgccgaaa aacaccgtt 
      catttgacg cggctcgtc ctctcggaa ggggtcgaa 
      cggccgaaa agtaccgac agtattgaa ttggaggaa 
      aacgcgcaa gatttgtgg gagattcac gagccggcg 
      accgaaaga ctgaaacac agcgcgacg tgcaagttc 
      gggctgttg aagcggcgc cagagcctg gactacggg 
      ggcttcgag gggctggag aacgccccc aagcaggag 
      tacaactac ggggaggtg tgttcgggg aacttcttc 
      catgtgccc cagttcgtc agctcgccg agcgccatt 
      ccgccccag tacgccccc aaggagcag gacttgtac 
      tactttcgg aaaaacaac cgctcgccg ctttacggc 
      cgcaagaac gaggagagc tttctcgag tgttcgagc 
      atacagagg ctgaagaac gacgagaag agtttgagt 
      gagagtctg ctctcgggt catgatgag agtatagac 
      gaactgtac ggttattac aatttaggg tgcgcgata 
      ggcgagtcg aaggtttcg aagaagaag gaagtcgag 
      ttgccgcct gtgataaag aagaagtct aggtgtagc 
      gagtgcaac aagcggttg aatatcacg aacatttac 
      gactgccga tgcgggaag atcttctgt tcgcagcat 
      aggtattcg gaggtgcac cgatgcagc tacgattac 
      aagacggaa gggaggcgg attttggag caccagaat 
      ccgcttgtt actgccgac aagattaac cgaatctaa
    mRNA sequence
    • >TC005738 RA
      tgacagctg tgaatcatt gccaacact aattttagc 
      tccgttacg tatgtttgc gttaatttt ttagttaca 
      taaacaaga ttgtctccc ggggggcgc acaccaccg 
      ctgccgtgc tccaccacc gttcgggcg cacccacag 
      tggcgcaag ccgcggaaa attccagga ctttgctcg 
      ggtcggttg caatgcaac gttgcgccc gtgcataaa 
      ataaatcaa agtgacttt gctatctgt caactgtca 
      tcgtttgaa aatttagaa aaatgtctg aagatgggt 
      tttccgacg gtttcgacg aacccacca tggaaatac 
      agatcgaca cgctcatgg gcacctcgt tcgacatgc 
      gggtctcgg cgaccgaca ccgtcaggg agatcaagc 
      agagaatcc agcgagtcg aaggtatcc cgatccacc 
      aacaaaacc tcatattcc aatccaagg aattgaaag 
      actctcgtc gattatgtg atgcaggca tcaaaaacg 
      gttcgataa taaaactag tgatagcga tgaaaggcg 
      gtccgatat caacccgtc gcttatcag ttagttgcg 
      aacaccaca tgatgttaa aagaactga aagaactct 
      tggaaaaca ccagggatg aaatcggcg ataaactcg 
      cccccggct caaaagtat cagttttag tgttcaaag 
      aaggcgaca ttttaaatt tacttcgag taattgaaa 
      acgaagacg gttcatatt ccccttaca gcgagaagc 
      caatatcgc ccccgagca agccacgga aggagtccc 
      aaatggcaa tgttcgaaa aactggtcg aagacaccg 
      atatgatga cgaaaatct cgaatttga gaaagaaaa 
      tggaagatt tgagtcttc gacgacagt cgcgcaacg 
      ttaaacaca aagacgagg aaaaagtct cctacaagt 
      tactgaccg agtgttcga acgatttac tcgacgtgg 
      acttgaaga tttttgaga agaatctcg aagaagaga 
      gccagtccg agaccgagg aagtggccc taaaagaca 
      agcaccgga tcaaggccc ggaagtcgg tctacagcg 
      aaagcaagg gggccttct cgcacacga agcaaaact 
      acgcccgca tccacaaga agacgcgcg aactgcggg 
      gcgaaggct cgagcaaag agggcgacc tgtgcgtcg 
      ccgtgccga aaaacaccg ttcatttga cgcggctcg 
      tcctctcgg aaggggtcg aacggccga aaagtaccg 
      acagtattg aattggagg aaaacgcgc aagatttgt 
      gggagattc acgagccgg cgaccgaaa gactgaaac 
      acagcgcga cgtgcaagt tcgggctgt tgaagcggc 
      gccagagcc tggactacg ggggcttcg aggggctgg 
      agaacgccc ccaagcagg agtacaact acggggagg 
      tgtgttcgg ggaacttct tccatgtgc cccagttcg 
      tcagctcgc cgagcgcca ttccgcccc agtacgccc 
      ccaaggagc aggacttgt actactttc ggaaaaaca 
      accgctcgc cgctttacg gccgcaaga acgaggaga 
      gctttctcg agtgttcga gcatacaga ggctgaaga 
      acgacgaga agagtttga gtgagagtc tgctctcgg 
      gtcatgatg agagtatag acgaactgt acggttatt 
      acaatttag ggtgcgcga taggcgagt cgaaggttt 
      cgaagaaga aggaagtcg agttgccgc ctgtgataa 
      agaagaagt ctaggtgta gcgagtgca acaagcggt 
      tgaatatca cgaacattt acgactgcc gatgcggga 
      agatcttct gttcgcagc ataggtatt cggaggtgc 
      accgatgca gctacgatt acaagacgg aagggaggc 
      ggattttgg agcaccaga atccgcttg ttactgccg 
      acaagatta accgaatct aaacgagtg cctggattt 
      attttattt ttctttttc ttcaaacat gctgtattg 
      tcgatatca aatataccg tttaccatt tttttgcct 
      atttgagtg ttgtatatt ggatttccg aggttaggg 
      attttggtt gttgagtca gcacaagtc gagctttaa 
      taacaattt agtggattg tgctattat aatatcact 
      catcgttat atattttgt aattgattg ttttacttt 
      atggtctaa ctaattaga atgatattc tgttttatt 
      atcgaaaac gttcaaatg ttcctagag taatgtttg 
      gaccgtcct aaaaaattt ataagacaa aacaaaaaa 
      atcattgta tactttgtg attatactg tgttaatac 
      ttttaatcg atgtaaata tgttatgta aaatataac 
      cccactcca act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY