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Gene information
General information
TC005721 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8512071..8513788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005721
    iBeetle screen iB_04002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005721 RA
      MSVSHNCDE NRRNLLSEV ESAISNNDL ATLKQLLPH 
      PFDQTTLQD ENGNNPLHL SIIYEKNAI FDYLLDKFD 
      LEAQNHSGH TALSLSLLY HSDIFIIEY FALELIRKG 
      ANIEVVFSD NNTVLHLAL QRHYFQVAQ LVIEKGADL 
      NVQNDAGCT PLHMLFHVK TDPEGVTEG VGLMLYHGA 
      DASVRDKNG NVPFELAVI YDRYIKVAQ ENLFFYTFD 
      SNSSYEMSD EVLFELLES KSSLFFQIV DYVSEVVFV 
      SGVTFSPFL LFRIDPNFL KVVIGKFDY VVRKILACP 
      PDHFNWFRF RIESLQNWE ILLASHLSA DTIEFIGRF 
      SISWLVKKL DEDQVPPKI ILGFLFYLL SYGLEVSEI 
      DVQAVYKKF GYCDLFKTF LHLDIKKSK LMNTDILPK 
      LIYDVSLDL GTFLTDPHD FSINAIENL LDYYADPQL 
      RQFCNKLGV KKLVVKSTA LPQVPLLVQ LARDTVRKY 
      LVEKFSIRH PKQFFSLLK QLPISHIHE KILSFETKL 
      Y
    CDS sequence
    • >TC005721 RA
      atgtctgtc tcacataat tgtgatgaa aaccgccga 
      aatctctta tctgaagtt gaatcagca atatctaac 
      aatgacctt gccaccctc aagcagctt ttaccgcac 
      ccttttgac caaaccacc cttcaggac gaaaacggc 
      aacaaccct ctgcactta tccataatt tacgaaaag 
      aacgcaatt tttgactac ttgcttgat aaattcgac 
      ttggaggcg caaaaccac tccggacac acagcccta 
      tccctgtcc ctgctctac cacagcgac attttcata 
      atcgagtac tttgccctg gaactgatc cgcaagggg 
      gccaacatt gaggtagtt tttagcgat aataacaca 
      gtactgcac ttggccctc caacgtcac tacttccaa 
      gtcgcccag cttgtaatt gaaaagggg gccgattta 
      aacgtgcaa aacgacgcg gggtgcacc cccttacac 
      atgcttttc cacgtaaag accgacccc gaaggggtg 
      actgagggc gtggggctc atgctgtac cacggggcg 
      gatgccagc gtccgtgac aagaacggg aacgtcccc 
      ttcgagctt gcggtaatt tatgatcgt tatataaaa 
      gtagcgcag gaaaatctg ttcttttac acgtttgat 
      tccaactcg agctacgaa atgtcggac gaggtgttg 
      ttcgagttg ttggagtcc aaatcatcg ttgtttttc 
      caaattgtt gattacgtt tccgaagtt gtttttgtc 
      tcaggtgtg acgttctcg ccgtttttg ttgtttcgg 
      atcgatccg aattttctt aaagttgta atcgggaaa 
      tttgactac gttgttcgg aaaatccta gcgtgtcca 
      cccgaccat ttcaattgg ttccggttc agaattgag 
      tcgcttcaa aattgggaa attctactc gcgagtcat 
      ttaagcgcg gacactatt gaattcatt ggcaggttt 
      agtatcagt tggttggtt aaaaagcta gatgaggat 
      caagtgcct ccgaaaata attttaggt tttttgttt 
      tacttgctt tcgtacggt ttggaggtt tccgaaatt 
      gacgtacag gctgtgtac aaaaaattt ggttactgt 
      gatcttttc aagactttc ttacacttg gatattaag 
      aaaagcaaa ttaatgaac accgatata ctcccgaaa 
      ttaatttac gacgttagt ttagacttg gggacgttc 
      ctcacagac cctcacgac ttttcaatc aatgcgatt 
      gaaaatttg ttggattat tacgccgac ccccaatta 
      agacaattt tgcaataaa ctgggagtc aaaaaactt 
      gtggttaaa tcaaccgct ttgccccaa gtgcccctc 
      cttgtgcaa ctagccaga gacacggtg cgcaaatac 
      ctggttgaa aaattcagt ataaggcac ccgaaacaa 
      tttttcagc ctacttaaa caactacca atcagccac 
      atccatgaa aaaatcctg tcgtttgaa accaaactg 
      tattga
    mRNA sequence
    • >TC005721 RA
      gctttcttc aaataattg ttacaaatg aaattgatg 
      gaaaattga tgaagatga ctcgtaaat cgtttaagt 
      gatgtctgt ctcacataa ttgtgatga aaaccgccg 
      aaatctctt atctgaagt tgaatcagc aatatctaa 
      caatgacct tgccaccct caagcagct tttaccgca 
      cccttttga ccaaaccac ccttcagga cgaaaacgg 
      caacaaccc tctgcactt atccataat ttacgaaaa 
      gaacgcaat ttttgacta cttgcttga taaattcga 
      cttggaggc gcaaaacca ctccggaca cacagccct 
      atccctgtc cctgctcta ccacagcga cattttcat 
      aatcgagta ctttgccct ggaactgat ccgcaaggg 
      ggccaacat tgaggtagt ttttagcga taataacac 
      agtactgca cttggccct ccaacgtca ctacttcca 
      agtcgccca gcttgtaat tgaaaaggg ggccgattt 
      aaacgtgca aaacgacgc ggggtgcac ccccttaca 
      catgctttt ccacgtaaa gaccgaccc cgaaggggt 
      gactgaggg cgtggggct catgctgta ccacggggc 
      ggatgccag cgtccgtga caagaacgg gaacgtccc 
      cttcgagct tgcggtaat ttatgatcg ttatataaa 
      agtagcgca ggaaaatct gttctttta cacgtttga 
      ttccaactc gagctacga aatgtcgga cgaggtgtt 
      gttcgagtt gttggagtc caaatcatc gttgttttt 
      ccaaattgt tgattacgt ttccgaagt tgtttttgt 
      ctcaggtgt gacgttctc gccgttttt gttgtttcg 
      gatcgatcc gaattttct taaagttgt aatcgggaa 
      atttgacta cgttgttcg gaaaatcct agcgtgtcc 
      acccgacca tttcaattg gttccggtt cagaattga 
      gtcgcttca aaattggga aattctact cgcgagtca 
      tttaagcgc ggacactat tgaattcat tggcaggtt 
      tagtatcag ttggttggt taaaaagct agatgagga 
      tcaagtgcc tccgaaaat aattttagg ttttttgtt 
      ttacttgct ttcgtacgg tttggaggt ttccgaaat 
      tgacgtaca ggctgtgta caaaaaatt tggttactg 
      tgatctttt caagacttt cttacactt ggatattaa 
      gaaaagcaa attaatgaa caccgatat actcccgaa 
      attaattta cgacgttag tttagactt ggggacgtt 
      cctcacaga ccctcacga cttttcaat caatgcgat 
      tgaaaattt gttggatta ttacgccga cccccaatt 
      aagacaatt ttgcaataa actgggagt caaaaaact 
      tgtggttaa atcaaccgc tttgcccca agtgcccct 
      ccttgtgca actagccag agacacggt gcgcaaata 
      cctggttga aaaattcag tataaggca cccgaaaca 
      atttttcag cctacttaa acaactacc aatcagcca 
      catccatga aaaaatcct gtcgtttga aaccaaact 
      gtattgaat aaaacaagc gagctttta taaactttt 
      at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY