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Gene information
General information
TC005714 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8566252..8570498
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005714
    iBeetle screen iB_04000

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs segment shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Adult morphology
    elytra pigmentation/tanning decreased - Number of animals showing the phenotype: 4


    elytra surface irregular - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005714 RA
      MEEIDGNAI LAQALKEQG IEYVFGICG FPVIELSMA 
      LQTAGIHYI GMRNEQAAC YAAQAIGYL TGVPGGVLV 
      VSGPGLLHV CAGMANAQV NCWPVVVIG GSCPQDHEG 
      IGGFQECYQ VELARPYCK YSARPPSLA LIPQHVEKA 
      VRLATYGRP GAVYLDFPG NLLQARTTI DKIPKQYTA 
      PEIPVIYPE PRKIDEAAA LIMGAQRPL VIVGKGAAY 
      ARAEGQVRE LVDSTNLPF LATPMGKGV VSDTNPNSI 
      QPARSLALQ RADVVLLLG ARLNWILHF GRPPRYNPN 
      VKVIQIDVC AEELNNSVK AAVAIQSDL KPAVGQLVQ 
      SLKRRNFTF SKNSDWWND LNKKIQDNK RKVQEMTLD 
      VSEPLNYYA VFHHLQEVL PQNPIIVSE GANTMDIGR 
      AVLMNGLPR HRLDAGTFG TMGVGLGFA IAAALYCRH 
      FEPKKRVIC VEGDSAFGF SGMEIETMV RYKLPVIIV 
      VVNNSGIYG GLPQEVYKD LQESGEVTK VTPPTTLFT 
      STHYEKMMT MFGRQGYHC TTIAELQKA VKEALKETE 
      GPSIINVII SPSADRKPQ DFNWLTESK L
    CDS sequence
    • >TC005714 RA
      atggaagaa atcgatgga aacgcaata cttgcccaa 
      gccctcaaa gaacagggc attgagtac gtcttcggc 
      atctgcggc tttccggta atcgagctc agcatggcc 
      ctccaaacg gccggcatc cactacatc ggcatgcgt 
      aacgagcaa gccgcctgc tacgccgct caggccatc 
      ggctaccta accggagtc cccggcggc gtcctcgtc 
      gtctccggc cccggcctc ctccacgtg tgcgccggc 
      atggccaac gcccaagtc aactgctgg cccgtggtg 
      gtcattggc ggctcctgc ccgcaggac cacgaaggc 
      atcggcggc ttccaggag tgctaccaa gtggagcta 
      gcccgccct tactgcaaa tactccgcc cggccgccc 
      agcctcgcc ctcatcccc cagcacgtg gaaaaggcc 
      gtgcgcttg gccacatac ggccgaccc ggagccgtc 
      tacttggac tttcccggg aacctgctc caggcccgc 
      accactatc gacaaaatc cccaagcag tacaccgcg 
      cctgaaatc ccggtgatt tacccggag ccgcggaaa 
      atcgacgag gccgccgct ttgatcatg ggggcccag 
      cgaccgctg gtgatagtc gggaaaggg gcggcgtat 
      gcacgtgcc gaggggcaa gtccgggag ttggtcgat 
      tcgaccaac ttacccttc ttggccacg ccgatggga 
      aaaggggtg gtttcggac accaaccct aactccatc 
      cagcctgct agatcccta gcgttgcaa cgagccgac 
      gttgttttg ttattaggg gctaggctg aactggatc 
      ttgcacttc gggcgcccc ccgaggtac aaccccaac 
      gtcaaggtc atccaaatt gatgtttgt gccgaagaa 
      ttgaacaac agtgtcaag gcggctgtg gccatccaa 
      tccgacctt aaaccagca gtggggcaa ttggtccaa 
      agcctcaaa aggcgtaac ttcaccttt agcaaaaac 
      tccgattgg tggaacgat cttaacaag aaaatccaa 
      gacaataag aggaaagtg caagaaatg acgcttgat 
      gtttccgaa cctttgaac tactatgcc gttttccac 
      cacttgcaa gaagttttg ccccagaac cccatcata 
      gtgagcgag ggcgccaat accatggat attgggcga 
      gctgtgcta atgaacggc ctaccacgg catagactg 
      gacgctggt acttttggt accatgggt gtgggtcta 
      ggttttgcc atcgcggcg gccctttat tgccgccat 
      tttgagccc aagaaaagg gttatttgt gtggagggc 
      gactcagcg tttggcttc tcggggatg gagattgag 
      accatggta cggtacaaa ctcccggtg attatagtc 
      gtggtcaac aactcgggg atttacgga ggcttgccc 
      caggaagtc tacaaggat ttgcaggag agtggagaa 
      gtcacaaaa gtgacacct ccgaccact ctgttcacg 
      agcacccat tacgagaaa atgatgacg atgtttggg 
      cgtcagggg taccactgc acgaccatt gccgagctg 
      cagaaagcc gtcaaggaa gcactgaag gagaccgaa 
      ggtcccagc attatcaac gttattatc agtccttca 
      gccgacaga aaaccgcaa gatttcaac tggttgact 
      gaatcaaaa ttgtaa
    mRNA sequence
    • >TC005714 RA
      cgaaaacga aaagtcatt cctacttac gtccaaaaa 
      gcaaatcat taaaactaa ttcaacgag aatccttat 
      ttttgattt agctgtaaa gataacgcc atttctttg 
      tggtccttt tgatttaaa aggcgcatc cagcaaaat 
      aataatgcc atttcccca cgttcccct accaggttg 
      ttattgtta agaaccgat aaacgtcaa gcaaggttt 
      gtgctgtgt tgttatcag agtgaaaca agtttttag 
      aggtaaaac agcgtaaaa taatggaag aaatcgatg 
      gaaacgcaa tacttgccc aagccctca aagaacagg 
      gcattgagt acgtcttcg gcatctgcg gctttccgg 
      taatcgagc tcagcatgg ccctccaaa cggccggca 
      tccactaca tcggcatgc gtaacgagc aagccgcct 
      gctacgccg ctcaggcca tcggctacc taaccggag 
      tccccggcg gcgtcctcg tcgtctccg gccccggcc 
      tcctccacg tgtgcgccg gcatggcca acgcccaag 
      tcaactgct ggcccgtgg tggtcattg gcggctcct 
      gcccgcagg accacgaag gcatcggcg gcttccagg 
      agtgctacc aagtggagc tagcccgcc cttactgca 
      aatactccg cccggccgc ccagcctcg ccctcatcc 
      cccagcacg tggaaaagg ccgtgcgct tggccacat 
      acggccgac ccggagccg tctacttgg actttcccg 
      ggaacctgc tccaggccc gcaccacta tcgacaaaa 
      tccccaagc agtacaccg cgcctgaaa tcccggtga 
      tttacccgg agccgcgga aaatcgacg aggccgccg 
      ctttgatca tgggggccc agcgaccgc tggtgatag 
      tcgggaaag gggcggcgt atgcacgtg ccgaggggc 
      aagtccggg agttggtcg attcgacca acttaccct 
      tcttggcca cgccgatgg gaaaagggg tggtttcgg 
      acaccaacc ctaactcca tccagcctg ctagatccc 
      tagcgttgc aacgagccg acgttgttt tgttattag 
      gggctaggc tgaactgga tcttgcact tcgggcgcc 
      ccccgaggt acaacccca acgtcaagg tcatccaaa 
      ttgatgttt gtgccgaag aattgaaca acagtgtca 
      aggcggctg tggccatcc aatccgacc ttaaaccag 
      cagtggggc aattggtcc aaagcctca aaaggcgta 
      acttcacct ttagcaaaa actccgatt ggtggaacg 
      atcttaaca agaaaatcc aagacaata agaggaaag 
      tgcaagaaa tgacgcttg atgtttccg aacctttga 
      actactatg ccgttttcc accacttgc aagaagttt 
      tgccccaga accccatca tagtgagcg agggcgcca 
      ataccatgg atattgggc gagctgtgc taatgaacg 
      gcctaccac ggcatagac tggacgctg gtacttttg 
      gtaccatgg gtgtgggtc taggttttg ccatcgcgg 
      cggcccttt attgccgcc attttgagc ccaagaaaa 
      gggttattt gtgtggagg gcgactcag cgtttggct 
      tctcgggga tggagattg agaccatgg tacggtaca 
      aactcccgg tgattatag tcgtggtca acaactcgg 
      ggatttacg gaggcttgc cccaggaag tctacaagg 
      atttgcagg agagtggag aagtcacaa aagtgacac 
      ctccgacca ctctgttca cgagcaccc attacgaga 
      aaatgatga cgatgtttg ggcgtcagg ggtaccact 
      gcacgacca ttgccgagc tgcagaaag ccgtcaagg 
      aagcactga aggagaccg aaggtccca gcattatca 
      acgttatta tcagtcctt cagccgaca gaaaaccgc 
      aagatttca actggttga ctgaatcaa aattgtaat 
      ttttgtact gttgttata ataaagtgt tggttaatt 
      a
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