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Gene information
General information
TC005652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10123952..10136169
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005652
    iBeetle screen iB_03995

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005652 RA
      MNFSSAIFL ILVAVCGAT ADCPAKKTQ SKLICYYSK 
      LTQADSCKC SHVILPADA DVKAIDRLR EHAKGVKIL 
      ITVNEFNQG LVNLLKSSK VDGIEINLK KLDSKNDIS 
      DFISTVRSK LGADLSIVL SVPSKAETL AKYYDFKAL 
      SKNADLFIL QTAFLGASK NVTFHPSRL SGLWDMQNT 
      DSVVDLVSG LGAPLSKLV ITAPVQAFK FKLQDEKHT 
      APGSPALEV QSITRDELC SVMGNGGNW TLERDQDQT 
      GSYIFSKDQ WIAFDDATS IDIKAKYAR VRGLAGMAL 
      KDLSQDGGT KCESSLLEA AFTGLSRQA RAPRGAVLH 
      SLEREILET PARPLDSIQ VSPYRISRV IDVEGNVHV 
      IRKDTRTEF ECSRQGYFV HPRSCGRFY RCVKFDQLS 
      DEFSVFEFD CPAGLAFDS RVEVCVWPG SLPHASACA 
      GSSEIAPVP RERFVCPNE PGYYADPEN CRWFFACLD 
      HGKSPLSAY EFRCPFGLV YDESRLLCE WPWLVPKCG 
      SGYAVGSEV VYGGAGSVT LDQYDGLGV LGVTALGGL 
      HGPLQQPVV YSNVGVGGL SVKSAQSLQ SAGLLKAGY 
      ADGNLAYSN VGNVGIDGS SFIGTQKHG FYRGDGYGV 
      KSQGFVHGS SHVHNGDAY LVNTGLYQG HVASDQTSN 
      YVHGTNLDY ETGVSGAAV KSAEGYQGA LHLVDGAKT 
      GYIDTANAH LVNGLAENY QQAVLVNQQ AGAYAQGAA 
      VSGYDSAKS SVEYHGNTG TTYGSGTSG SINIVGGGY 
      SAVGVATDG VQTNYADTV AHVTQSPIA VSSIPTVTS 
      VPVPALKVA SGFESYNGG VVANVPELQ YKLQSSSHG 
      TRGFANISD SFVNFVSST PSTIAVEDG YHYPKPTVA 
      FVEGPVKSV STGTSFVQQ NIASVQPVV TTYQKPVFV 
      STTQRPLLV EKVNVQPVV TTYQKPVQP VVTTYQKPV 
      TTSFVQHVQ PVVTTYQKP VIVSSTPAP VVVEKVNIQ 
      PVVTTYEKP VFVSTPAPI DIQPVVAKE SFVGYDYPK 
      PAVKFEEVP VKTTGGSFS YQNVDLIHK TEGYTYPKP 
      AVAFEEVPV KTYKQEAVS SFTYSTPST VRPVVVHKV 
      QPVVQPQIH VTSYNVPST TVKPVIVEQ PQVYVSSTP 
      APIQPVVKQ QAVSFLNYQ KHETVQPQL EVTAYNLPS 
      STIRPVVTP VVHQKPAVV SSFSISSNS EGYHYEKPK 
      IVFDERPVV TYQQPAVQK TVVATQPVY STYNYQTPV 
      STYNYKNVV KSAVNLGYS YPKPAIAFE ETPASVTRL 
      SYTQPAVFS SYSYAAPTV QPVVVQKQA PVVTSYTYN 
      APSTAKPAV YVQQPVVNT YVSSTPRPE ILIHKQPVV 
      TTYVSSTPK PAVFYQQQP VVTTYASTP KPAVFFQQQ 
      PVVTTYAST PKPKVFFQQ QPVVTYTQK PVVTTYVST 
      PKPEIFVQK QPVVTYTQK PVVTTYVSS TPKPEIYVQ 
      KQPAVVSSF SFTNNVQGY DYPKPVVPF VEEPAPVVT 
      SYENRQPVE EVPFVKKKA YVTGQQTNY QYVEQPRFE 
      YKQPVVVST TPRYEYKQQ YSVDSGYIY DKPAVRFEE 
      KPAFVKVST PKAIVDYTY VQSTPKPTF ASTITEVVA 
      AKQPFTFYD SRLHSTPKP VVYSTQLPV VETVSTSRP 
      ISKYAFSSL NEYNKIASY VPPAKIVTP TVVTTSRPI 
      VKYSFTSQP RVVENYVAP IEVTTPTRD YLPIKVTTS 
      APTEAYVVP EVVKVTTSR PRVKVASLS TVVPEVPTR 
      EYLPVRTRV KVTSLPTET YVVPEATTR GYLPPRTRV 
      KVTPSSTYL PVEEELVST SRPVVKVAR PKTIIKVND 
      FHPLLSAKL GAQCTCTSN TVRLRKKKL RIVVEDDDD 
      DGYVVDDNN EGVVVENYQ YEPQKLDIT PTPEIYIKS 
      TTSHIVEPT PSPTYSTRR RVRVRPATS TVLYDSPVT 
      QAAEIFVKK AVTPAVVVN AVTDDDAKL AKTSVESRT 
      FDRYGPGGW RSRNEKLQG TIDCQRAGL FRHPTQCNK 
      FYACRWDCT KNRFTLHVF NCPVHLTFD NTLGACNWP 
      SQGPACLEN TLLPSD
    CDS sequence
    • >TC005652 RA
      atgaacttt tcaagtgcc atatttttg atccttgtc 
      gcggtctgc ggggccacg gccgactgt cccgcgaaa 
      aaaactcag tcgaaactg atctgttat tatagtaaa 
      ttgactcag gccgattcc tgcaaatgt tcacatgtt 
      atcctgccg gcggacgcc gacgtcaag gccatcgac 
      aggctcagg gagcacgct aagggcgtc aaaattttg 
      atcaccgtc aacgaattc aatcagggc ttggtcaac 
      ctcctgaaa agcagtaaa gtggacggt attgaaatc 
      aacctgaag aaactcgat tcgaaaaac gacatcagc 
      gacttcatc tcgaccgtc cgaagcaag ctcggcgcc 
      gacttgtcc atcgtcctt tctgtgcct tcaaaagcc 
      gagaccttg gcaaaatac tacgacttt aaggctttg 
      agcaaaaat gccgatctg ttcatcctg caaacagcg 
      ttccttggc gcttccaag aacgtcact ttccacccc 
      agccggctt tccggcctt tgggacatg cagaatact 
      gattccgtg gtggatctg gtgagcggc ttgggggcc 
      cctttgtcc aaactggtg ataacagca cccgtccaa 
      gctttcaag ttcaagctc caggacgag aaacacaca 
      gctccgggg agtccggcg ctggaagtc cagtcaatt 
      acgagagat gagctttgc agtgttatg gggaatggc 
      gggaactgg acgctggag cgggaccag gaccagact 
      gggtcctac atatttagt aaagaccag tggatcgct 
      ttcgacgac gccacgagc attgacatc aaggccaag 
      tacgcgaga gtccggggc ttggccgga atggccctt 
      aaggacctc tcgcaggac ggcggcacc aagtgcgaa 
      agctcgctc ctggaggca gctttcacg ggtttgtcg 
      cggcaagcg cgcgcccca cgcggcgcc gtgttgcac 
      tccctggag cgcgagatc ctggaaacg ccagctcgg 
      cccctggac agcatccag gtgtcgcct taccgcatc 
      tccagggtt atagacgtt gaggggaac gtgcacgtt 
      attagaaag gatacaagg acggagttt gagtgttcg 
      cggcaaggg tatttcgtc catccgcgg tcctgtggc 
      cggttctac cgttgtgtc aagttcgac caactttcc 
      gacgagttt agcgttttc gaattcgat tgtcctgca 
      ggactggct tttgatagt cgagtggag gtttgtgtt 
      tggcccgga agtttgccg catgcttct gcttgtgcc 
      ggaagtagt gaaattgcg cccgtgccg agggaaaga 
      ttcgtttgt cccaatgaa ccaggttat tacgcggat 
      cccgaaaac tgtcgctgg ttctttgcg tgtttggac 
      cacggaaaa tctccattg tccgcctat gaatttaga 
      tgtcccttc ggattagtt tacgacgag tcaagactt 
      ctctgtgaa tggccttgg ttggtacca aaatgtggc 
      agcggttat gcagttggt tctgaagtg gtttatgga 
      ggtgcagga tcggttact ctcgatcag tatgatggt 
      ttgggcgtt cttggagta acagctctt ggtggactt 
      cacggtccg ttgcaacag cctgtggtt tatagcaac 
      gtgggtgtt ggaggatta tccgtcaaa agtgcacaa 
      tccttgcaa agtgccgga ctcctcaag gctggatat 
      gccgatggt aatcttgct tactcaaac gtgggtaat 
      gtagggatt gatggttca tctttcatt ggtacacaa 
      aaacatggt ttttatcgc ggtgatggg tacggggtt 
      aaatcccaa ggttttgtt catggatcg agtcatgtt 
      cataatggt gacgcttat ttggtaaac acaggactt 
      taccaagga catgtagct tcagatcag acatctaat 
      tatgtacat ggtactaat ttagattac gaaacaggt 
      gtgtcaggt gctgctgtt aaatccgca gaaggatat 
      cagggtgct cttcaccta gttgatgga gctaagact 
      ggctacatc gatactgcc aatgcccat ctagtcaat 
      ggtcttgca gaaaattac caacaagct gttttggta 
      aatcaacaa gctggtgcc tatgcacaa ggagctgca 
      gtctctggt tatgatagc gctaaatcc tctgttgaa 
      tatcatgga aacactgga accacctat ggaagtggt 
      accagcgga agtataaat atcgtcgga ggaggatac 
      tcagcagtt ggagtagcc actgacggt gttcaaacc 
      aattatgcc gatacagta gcccacgtg acccaatct 
      ccaattgct gtctcctcc atccctact gtaacttcc 
      gttccagta ccagcactt aaagttgcc tcaggattt 
      gaaagctac aacggtgga gttgtcgca aacgtgccc 
      gaactgcag tacaaactc cagtcctcg tcacatgga 
      actagaggt tttgcgaat atttcagac agtttcgtc 
      aattttgtc tcatcaact ccgtcaaca attgcagta 
      gaggatggt tatcattac cctaaacct acagtcgct 
      tttgttgaa ggaccagtt aagtctgta tcaactggt 
      acctccttc gtccaacaa aatatcgct agtgttcaa 
      ccagttgtc acaacttac caaaagcct gtatttgtt 
      tcaacaaca caaagacca ctacttgtt gaaaaagtg 
      aacgtacaa ccggttgta actacatat caaaaacca 
      gttcaacca gtcgtaacc acatatcag aaaccagtc 
      acaacttcg tttgtacaa catgtccaa ccagtagtt 
      acaacatat caaaaacct gtaattgta tcatcaaca 
      ccagctcca gtagtagtt gaaaaagtt aacattcaa 
      cctgtggtt acaacgtat gagaaacct gtatttgtt 
      tcaacaccg gctcctatt gatattcaa ccagttgtt 
      gcaaaagaa agttttgtt gggtacgac taccccaaa 
      cctgctgtt aaatttgaa gaagtacca gtaaaaacc 
      accggtggt agctttagc tatcaaaat gttgatttg 
      attcacaag actgaaggt tacacttat cctaaacca 
      gcggtagct tttgaagaa gtaccggtg aaaacttac 
      aaacaagaa gcagtatcg tctttcact tattcaact 
      ccgtcaacc gttagacct gtggttgta cacaaagtt 
      caaccagtt gttcaaccg caaatccat gtaacgtct 
      tacaacgta ccgtcaaca acagtcaaa ccagtaatt 
      gttgaacaa ccacaagtt tacgtgtca tcaacacct 
      gcccctatt caaccagtg gtcaaacaa caagctgta 
      tcattttta aattaccag aaacatgaa actgttcaa 
      cctcaactt gaagtaacc gcctacaat ttaccatca 
      tcaactatc agacctgta gttactcct gtcgtccac 
      cagaaacca gctgttgta tccagtttt agtatatca 
      agcaacagt gaaggttat cattacgaa aaacctaaa 
      attgttttt gacgaaaga cctgtagtc acgtatcaa 
      caaccagca gtacaaaaa accgtagta gccactcaa 
      ccagtctat tcaacctat aattaccaa acaccagtt 
      agtacttac aactacaag aacgtggtg aaatcagca 
      gttaacctt ggatattca tacccgaaa cctgcaatt 
      gcttttgaa gaaacgcca gcttcagtt accagactg 
      agttacact caacctgcc gtattctca agttatagt 
      tacgcggct ccaacggta caacccgtt gtagtccaa 
      aaacaagca cctgtggtt actagctac acgtacaac 
      gcaccatcg actgccaaa cctgcagtt tacgtacaa 
      cagcctgtt gtaaacacc tacgtttcg tctacaccc 
      agacccgaa atcttaata cataaacaa cctgtcgta 
      accacctat gtatcatct acacctaaa cctgcagtc 
      ttctaccaa cagcaacct gtagtaacc acttacgca 
      tctacacct aaacctgca gtctttttc caacaacaa 
      cctgtagta accacttac gcatctaca cccaaacct 
      aaagtcttc ttccaacaa caacctgtg gttacatac 
      actcaaaaa cctgtcgtc accacttac gtatccaca 
      cccaaacct gaaatcttt gtccaaaaa caaccagtt 
      gttacgtac actcagaaa cctgtggta accacttat 
      gtctcctct acacctaaa cccgaaatc tacgtccag 
      aaacaacca gctgttgtt tccagcttc agttttaca 
      aataatgtg caaggttac gattatcca aaaccagtg 
      gttccgttc gtggaagaa ccagctccg gttgtaacg 
      tcttacgaa aatcgtcaa ccggttgag gaagtacca 
      tttgtaaag aagaaggcg tacgttact ggacaacaa 
      accaattac caatacgtg gaacaacca cgatttgag 
      tacaaacaa ccagttgtg gtatcgaca actccacga 
      tatgagtat aaacaacaa tatagtgtt gattctggt 
      tatatctac gataaacca gcagtgcgc tttgaagaa 
      aaacccgct tttgtcaaa gtgtcaact ccgaaagct 
      atagtcgac tacacctac gtccaatca actccaaaa 
      ccgacgttt gcgtccact attaccgaa gttgtggcc 
      gccaaacaa ccgtttact ttctacgac agtagactg 
      cactcaact cctaaaccc gtggtctac tcaacgcaa 
      cttccagtt gttgaaacg gtttcaaca agtcgaccg 
      atttcaaaa tacgctttc agttcgctt aacgaatac 
      aacaaaatt gcgagttac gtaccaccg gctaaaata 
      gtaacacca acagtggtt acgacaagc cgaccaata 
      gtcaagtac tcgttcacc tcgcaaccc cgagttgtt 
      gaaaattat gttgcacca attgaagta actacacca 
      acacgtgac tacttgccg atcaaggtg acaactagt 
      gcaccaacc gaggcatat gtagtacca gaggttgta 
      aaagttaca acgtcaaga cctagagtt aaggttgca 
      agtctatca actgtagta ccagaagta ccaacacgt 
      gaatatttg ccagtgaga acacgagtt aaagttaca 
      agcctacca actgaaact tacgtagta ccagaagcc 
      acaacacgt gggtatttg ccgccgaga acacgagtc 
      aaggtgact ccaagtagt acctacttg ccagttgag 
      gaagaactg gtttcgacc agcaggccg gttgtaaaa 
      gtcgcaaga ccgaagact ataatcaaa gtcaatgac 
      ttccacccg ttactctcg gcaaaacta ggagcacaa 
      tgcacctgc acgtcaaac acagtacga cttcgcaag 
      aagaagttg cgaattgtt gtagaagat gatgatgac 
      gatggttac gttgtcgat gacaacaac gagggcgtt 
      gttgtggaa aactaccaa tacgagccc caaaaactc 
      gacataacc ccgaccccc gaaatctac atcaaaagc 
      accactagt cacatagtc gaaccgacc ccctccccc 
      acctacagc accaggaga cgcgtccgc gtccgacct 
      gccacgtcc accgtcctg tacgactcc ccggtgacc 
      caagccgct gaaatcttc gtcaagaaa gcagtaacc 
      ccggctgtt gtcgtaaac gcagtgact gacgatgac 
      gcaaaactg gccaaaacg tcggtcgaa agccgcacc 
      ttcgaccgc tacggcccc ggcggttgg aggagtcgc 
      aacgaaaag ttgcaagga acgatcgac tgtcaaaga 
      gctgggctc ttccgacac ccgactcag tgcaacaaa 
      ttctacgcc tgtcggtgg gattgcacc aaaaaccgg 
      ttcacactg catgttttc aactgtccg gtccacctt 
      acgttcgac aatactctg ggcgcttgc aactggcca 
      agtcaggga ccggcgtgc ttggagaac accctcctc 
      cccagtgat tag
    mRNA sequence
    • >TC005652 RA
      aatggcaca aggcgaaac tgcaccaag acaaaataa 
      aactgctct ttggccggc tccgggcgt cgttacaaa 
      acggcgtta gtcaccaaa tcaaattcc gggatccgg 
      gcgtgaaac cggggcaag aattcggac tttgcggtg 
      ttgaaatgt gactccggt cacgacagt ctggtcccc 
      ggattatgc aacagctgg aaactgggc cagttgcac 
      tcggcggtc aagaaaatt ccaggcagg aaacggcga 
      aaattgggt gggtgtgaa agtgaaacc gatgggtgt 
      ttgatgggt tacgtgcag gaacaggtg catgggccc 
      gaatattga tgaactggt cggagaaga gacaagaaa 
      gtgattttc ttttgatag ccgttattt atgcaatgt 
      tctcacgag tggcataac accatggta ttttgatga 
      cagaggact acataatgc gattaagag aaggagagc 
      accctacat tagcccaag tgaccgcca aaggatcag 
      ttaatcttc ggcgatcgg cttaataag atcggccgc 
      caaaaaaga gcgaaatca ctttcagtt tcgcagtga 
      cttcaacgg tagatcaac acatgtgcg cgggatgac 
      gagactttg atacttttg ccagtttaa acgcatcaa 
      agttgacga aatgaactt ttcaagtgc catattttt 
      gatccttgt cgcggtctg cggggccac ggccgactg 
      tcccgcgaa aaaaactca gtcgaaact gatctgtta 
      ttatagtaa attgactca ggccgattc ctgcaaatg 
      ttcacatgt tatcctgcc ggcggacgc cgacgtcaa 
      ggccatcga caggctcag ggagcacgc taagggcgt 
      caaaatttt gatcaccgt caacgaatt caatcaggg 
      cttggtcaa cctcctgaa aagcagtaa agtggacgg 
      tattgaaat caacctgaa gaaactcga ttcgaaaaa 
      cgacatcag cgacttcat ctcgaccgt ccgaagcaa 
      gctcggcgc cgacttgtc catcgtcct ttctgtgcc 
      ttcaaaagc cgagacctt ggcaaaata ctacgactt 
      taaggcttt gagcaaaaa tgccgatct gttcatcct 
      gcaaacagc gttccttgg cgcttccaa gaacgtcac 
      tttccaccc cagccggct ttccggcct ttgggacat 
      gcagaatac tgattccgt ggtggatct ggtgagcgg 
      cttgggggc ccctttgtc caaactggt gataacagc 
      acccgtcca agctttcaa gttcaagct ccaggacga 
      gaaacacac agctccggg gagtccggc gctggaagt 
      ccagtcaat tacgagaga tgagctttg cagtgttat 
      ggggaatgg cgggaactg gacgctgga gcgggacca 
      ggaccagac tgggtccta catatttag taaagacca 
      gtggatcgc tttcgacga cgccacgag cattgacat 
      caaggccaa gtacgcgag agtccgggg cttggccgg 
      aatggccct taaggacct ctcgcagga cggcggcac 
      caagtgcga aagctcgct cctggaggc agctttcac 
      gggtttgtc gcggcaagc gcgcgcccc acgcggcgc 
      cgtgttgca ctccctgga gcgcgagat cctggaaac 
      gccagctcg gcccctgga cagcatcca ggtgtcgcc 
      ttaccgcat ctccagggt tatagacgt tgaggggaa 
      cgtgcacgt tattagaaa ggatacaag gacggagtt 
      tgagtgttc gcggcaagg gtatttcgt ccatccgcg 
      gtcctgtgg ccggttcta ccgttgtgt caagttcga 
      ccaactttc cgacgagtt tagcgtttt cgaattcga 
      ttgtcctgc aggactggc ttttgatag tcgagtgga 
      ggtttgtgt ttggcccgg aagtttgcc gcatgcttc 
      tgcttgtgc cggaagtag tgaaattgc gcccgtgcc 
      gagggaaag attcgtttg tcccaatga accaggtta 
      ttacgcgga tcccgaaaa ctgtcgctg gttctttgc 
      gtgtttgga ccacggaaa atctccatt gtccgccta 
      tgaatttag atgtccctt cggattagt ttacgacga 
      gtcaagact tctctgtga atggccttg gttggtacc 
      aaaatgtgg cagcggtta tgcagttgg ttctgaagt 
      ggtttatgg aggtgcagg atcggttac tctcgatca 
      gtatgatgg tttgggcgt tcttggagt aacagctct 
      tggtggact tcacggtcc gttgcaaca gcctgtggt 
      ttatagcaa cgtgggtgt tggaggatt atccgtcaa 
      aagtgcaca atccttgca aagtgccgg actcctcaa 
      ggctggata tgccgatgg taatcttgc ttactcaaa 
      cgtgggtaa tgtagggat tgatggttc atctttcat 
      tggtacaca aaaacatgg tttttatcg cggtgatgg 
      gtacggggt taaatccca aggttttgt tcatggatc 
      gagtcatgt tcataatgg tgacgctta tttggtaaa 
      cacaggact ttaccaagg acatgtagc ttcagatca 
      gacatctaa ttatgtaca tggtactaa tttagatta 
      cgaaacagg tgtgtcagg tgctgctgt taaatccgc 
      agaaggata tcagggtgc tcttcacct agttgatgg 
      agctaagac tggctacat cgatactgc caatgccca 
      tctagtcaa tggtcttgc agaaaatta ccaacaagc 
      tgttttggt aaatcaaca agctggtgc ctatgcaca 
      aggagctgc agtctctgg ttatgatag cgctaaatc 
      ctctgttga atatcatgg aaacactgg aaccaccta 
      tggaagtgg taccagcgg aagtataaa tatcgtcgg 
      aggaggata ctcagcagt tggagtagc cactgacgg 
      tgttcaaac caattatgc cgatacagt agcccacgt 
      gacccaatc tccaattgc tgtctcctc catccctac 
      tgtaacttc cgttccagt accagcact taaagttgc 
      ctcaggatt tgaaagcta caacggtgg agttgtcgc 
      aaacgtgcc cgaactgca gtacaaact ccagtcctc 
      gtcacatgg aactagagg ttttgcgaa tatttcaga 
      cagtttcgt caattttgt ctcatcaac tccgtcaac 
      aattgcagt agaggatgg ttatcatta ccctaaacc 
      tacagtcgc ttttgttga aggaccagt taagtctgt 
      atcaactgg tacctcctt cgtccaaca aaatatcgc 
      tagtgttca accagttgt cacaactta ccaaaagcc 
      tgtatttgt ttcaacaac acaaagacc actacttgt 
      tgaaaaagt gaacgtaca accggttgt aactacata 
      tcaaaaacc agttcaacc agtcgtaac cacatatca 
      gaaaccagt cacaacttc gtttgtaca acatgtcca 
      accagtagt tacaacata tcaaaaacc tgtaattgt 
      atcatcaac accagctcc agtagtagt tgaaaaagt 
      taacattca acctgtggt tacaacgta tgagaaacc 
      tgtatttgt ttcaacacc ggctcctat tgatattca 
      accagttgt tgcaaaaga aagttttgt tgggtacga 
      ctaccccaa acctgctgt taaatttga agaagtacc 
      agtaaaaac caccggtgg tagctttag ctatcaaaa 
      tgttgattt gattcacaa gactgaagg ttacactta 
      tcctaaacc agcggtagc ttttgaaga agtaccggt 
      gaaaactta caaacaaga agcagtatc gtctttcac 
      ttattcaac tccgtcaac cgttagacc tgtggttgt 
      acacaaagt tcaaccagt tgttcaacc gcaaatcca 
      tgtaacgtc ttacaacgt accgtcaac aacagtcaa 
      accagtaat tgttgaaca accacaagt ttacgtgtc 
      atcaacacc tgcccctat tcaaccagt ggtcaaaca 
      acaagctgt atcattttt aaattacca gaaacatga 
      aactgttca acctcaact tgaagtaac cgcctacaa 
      tttaccatc atcaactat cagacctgt agttactcc 
      tgtcgtcca ccagaaacc agctgttgt atccagttt 
      tagtatatc aagcaacag tgaaggtta tcattacga 
      aaaacctaa aattgtttt tgacgaaag acctgtagt 
      cacgtatca acaaccagc agtacaaaa aaccgtagt 
      agccactca accagtcta ttcaaccta taattacca 
      aacaccagt tagtactta caactacaa gaacgtggt 
      gaaatcagc agttaacct tggatattc atacccgaa 
      acctgcaat tgcttttga agaaacgcc agcttcagt 
      taccagact gagttacac tcaacctgc cgtattctc 
      aagttatag ttacgcggc tccaacggt acaacccgt 
      tgtagtcca aaaacaagc acctgtggt tactagcta 
      cacgtacaa cgcaccatc gactgccaa acctgcagt 
      ttacgtaca acagcctgt tgtaaacac ctacgtttc 
      gtctacacc cagacccga aatcttaat acataaaca 
      acctgtcgt aaccaccta tgtatcatc tacacctaa 
      acctgcagt cttctacca acagcaacc tgtagtaac 
      cacttacgc atctacacc taaacctgc agtcttttt 
      ccaacaaca acctgtagt aaccactta cgcatctac 
      acccaaacc taaagtctt cttccaaca acaacctgt 
      ggttacata cactcaaaa acctgtcgt caccactta 
      cgtatccac acccaaacc tgaaatctt tgtccaaaa 
      acaaccagt tgttacgta cactcagaa acctgtggt 
      aaccactta tgtctcctc tacacctaa acccgaaat 
      ctacgtcca gaaacaacc agctgttgt ttccagctt 
      cagttttac aaataatgt gcaaggtta cgattatcc 
      aaaaccagt ggttccgtt cgtggaaga accagctcc 
      ggttgtaac gtcttacga aaatcgtca accggttga 
      ggaagtacc atttgtaaa gaagaaggc gtacgttac 
      tggacaaca aaccaatta ccaatacgt ggaacaacc 
      acgatttga gtacaaaca accagttgt ggtatcgac 
      aactccacg atatgagta taaacaaca atatagtgt 
      tgattctgg ttatatcta cgataaacc agcagtgcg 
      ctttgaaga aaaacccgc ttttgtcaa agtgtcaac 
      tccgaaagc tatagtcga ctacaccta cgtccaatc 
      aactccaaa accgacgtt tgcgtccac tattaccga 
      agttgtggc cgccaaaca accgtttac tttctacga 
      cagtagact gcactcaac tcctaaacc cgtggtcta 
      ctcaacgca acttccagt tgttgaaac ggtttcaac 
      aagtcgacc gatttcaaa atacgcttt cagttcgct 
      taacgaata caacaaaat tgcgagtta cgtaccacc 
      ggctaaaat agtaacacc aacagtggt tacgacaag 
      ccgaccaat agtcaagta ctcgttcac ctcgcaacc 
      ccgagttgt tgaaaatta tgttgcacc aattgaagt 
      aactacacc aacacgtga ctacttgcc gatcaaggt 
      gacaactag tgcaccaac cgaggcata tgtagtacc 
      agaggttgt aaaagttac aacgtcaag acctagagt 
      taaggttgc aagtctatc aactgtagt accagaagt 
      accaacacg tgaatattt gccagtgag aacacgagt 
      taaagttac aagcctacc aactgaaac ttacgtagt 
      accagaagc cacaacacg tgggtattt gccgccgag 
      aacacgagt caaggtgac tccaagtag tacctactt 
      gccagttga ggaagaact ggtttcgac cagcaggcc 
      ggttgtaaa agtcgcaag accgaagac tataatcaa 
      agtcaatga cttccaccc gttactctc ggcaaaact 
      aggagcaca atgcacctg cacgtcaaa cacagtacg 
      acttcgcaa gaagaagtt gcgaattgt tgtagaaga 
      tgatgatga cgatggtta cgttgtcga tgacaacaa 
      cgagggcgt tgttgtgga aaactacca atacgagcc 
      ccaaaaact cgacataac cccgacccc cgaaatcta 
      catcaaaag caccactag tcacatagt cgaaccgac 
      cccctcccc cacctacag caccaggag acgcgtccg 
      cgtccgacc tgccacgtc caccgtcct gtacgactc 
      cccggtgac ccaagccgc tgaaatctt cgtcaagaa 
      agcagtaac cccggctgt tgtcgtaaa cgcagtgac 
      tgacgatga cgcaaaact ggccaaaac gtcggtcga 
      aagccgcac cttcgaccg ctacggccc cggcggttg 
      gaggagtcg caacgaaaa gttgcaagg aacgatcga 
      ctgtcaaag agctgggct cttccgaca cccgactca 
      gtgcaacaa attctacgc ctgtcggtg ggattgcac 
      caaaaaccg gttcacact gcatgtttt caactgtcc 
      ggtccacct tacgttcga caatactct gggcgcttg 
      caactggcc aagtcaggg accggcgtg cttggagaa 
      caccctcct ccccagtga ttagtctta agttctagt 
      atttattgc gcattgtcc atcatttta tttattaca 
      tcacccatc acgaaattc agctttttg ggtcttgga 
      ttgtatttt gtaaaataa aaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY