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Gene information
General information
TC005640 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10409397..10414088
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005640
    iBeetle screen iB_03994

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005640 RA
      MVARLLSTL LVLHSISVL LPSSCCRKK EPKLPVLQL 
      HTNDSLPAD LQVTVGLPA GRNKSYSYT SPSAHGALS 
      VTLTACTSP IFWSFGPDN TTMLHTHSM DTYHVPSPS 
      QGVYTLTLL APESATLAH IYISTERGG PQALQSAKF 
      SKVRLLKRQ KGKRLTVKW NPSLVDPQG TDYCLVVGT 
      KPPVGSLCA AQQDPALAV ACVGRKTHH TITGLKHSQ 
      KYYFSVFAI NNQSNFTYP YGTISSVFD GRFRHTSLK 
      DGKATFANL KKLDGKAVF RYKVVNATE SLDLFVIPC 
      GGAIDIEVT LKNATVVPP KRVKSFGRI TIKNPVQFA 
      RYYIKIYAL NREELKKTS GVEIYATTR FPTKIPLPN 
      MPQTLKVTE YKGLTTCDS VTVAWMAAP GQKSAHYCL 
      SATEGKIRE MEDYRMPNQ CGLESRLKK SVDFAVKFC 
      TDEKNYKEP VVYKTINQL KPGRNYIIQ VTVKKPKGK 
      TLSYDLLQV HTKPSCRRK QE
    CDS sequence
    • >TC005640 RA
      atggtggcc cgtttgctg agcaccctg ttggtcctg 
      cactccatt tcggtgctg ctccccagc agctgctgc 
      cgcaagaag gagcccaaa ctgcccgtc ctccagctc 
      cacaccaac gactccctg cccgccgac ctccaggtc 
      accgtcggg ctgcccgcc ggccgcaac aaaagttac 
      tcgtacaca tcgcccagc gcccacggc gcgctgtcc 
      gtcaccctc acggcgtgc acgtcgccc attttttgg 
      tccttcggg ccggataac accaccatg ctgcacacc 
      cactccatg gacacctac catgtgccg tctcccagc 
      cagggcgtg tacacgctc accctgctg gccccagaa 
      tccgccacc ctggcccac atctacata agcacggag 
      cgtggcgga ccccaagcg ctccagagc gccaaattc 
      tccaaagtc cgtctgctc aagcgccag aaagggaag 
      cggctaact gtgaagtgg aatccgagc ctggtggac 
      ccgcaaggt accgactat tgtctggtg gtgggtacc 
      aagccgccc gttgggtcg ctgtgtgcc gcacaacag 
      gacccagca ctggctgtg gcttgtgtt ggccgcaag 
      acccatcac accatcact ggtttaaag cacagccaa 
      aagtactac tttagcgtc ttcgcgatc aacaaccaa 
      tcgaatttt acgtaccct tacgggacc atatcttca 
      gttttcgac ggccggttc agacacacc agtctcaaa 
      gacgggaaa gccacgttt gccaattta aaaaaactc 
      gacggaaaa gcagttttt cgctacaaa gttgttaat 
      gcaacggag agtctagac ttattcgtg atcccatgt 
      gggggtgcg attgatatc gaagtaaca ttaaaaaac 
      gcaacggtg gtaccaccg aaacgagtc aaaagtttc 
      ggcagaatc actataaaa aatccagta cagttcgcc 
      cggtactac atcaagatt tatgccctg aaccgcgaa 
      gaattgaag aagacatcg ggagttgaa atttatgca 
      accacacgg tttccgacc aaaattccc ttaccaaac 
      atgccccaa accctcaaa gtgacagaa tacaagggt 
      ttgaccact tgtgatagt gtgacagta gcttggatg 
      gcagcgcca gggcagaaa agtgcccat tactgcctt 
      tcggcaacc gaaggaaaa atccgggag atggaagac 
      tacagaatg cccaaccaa tgcgggctt gaaagtcgc 
      ttgaaaaaa tcagtcgac tttgctgtg aaattttgc 
      acagatgag aaaaattac aaagaacct gtggtttac 
      aaaacaata aatcagcta aagcccggt cgcaattac 
      ataatccaa gtaactgtc aaaaagcca aaagggaaa 
      accttgtcg tatgatctt ctccaagtg catacgaaa 
      ccgtcctgc agaaggaag caagagtga
    mRNA sequence
    • >TC005640 RA
      caaggagtc cgctgaaag aaattacag gcagtgagc 
      tttcaggcc tgaagctga aaagtgaca attgtgtgg 
      tgctttttc gggacgtta aggtcaaga tggtggccc 
      gtttgctga gcaccctgt tggtcctgc actccattt 
      cggtgctgc tccccagca gctgctgcc gcaagaagg 
      agcccaaac tgcccgtcc tccagctcc acaccaacg 
      actccctgc ccgccgacc tccaggtca ccgtcgggc 
      tgcccgccg gccgcaaca aaagttact cgtacacat 
      cgcccagcg cccacggcg cgctgtccg tcaccctca 
      cggcgtgca cgtcgccca ttttttggt ccttcgggc 
      cggataaca ccaccatgc tgcacaccc actccatgg 
      acacctacc atgtgccgt ctcccagcc agggcgtgt 
      acacgctca ccctgctgg ccccagaat ccgccaccc 
      tggcccaca tctacataa gcacggagc gtggcggac 
      cccaagcgc tccagagcg ccaaattct ccaaagtcc 
      gtctgctca agcgccaga aagggaagc ggctaactg 
      tgaagtgga atccgagcc tggtggacc cgcaaggta 
      ccgactatt gtctggtgg tgggtacca agccgcccg 
      ttgggtcgc tgtgtgccg cacaacagg acccagcac 
      tggctgtgg cttgtgttg gccgcaaga cccatcaca 
      ccatcactg gtttaaagc acagccaaa agtactact 
      ttagcgtct tcgcgatca acaaccaat cgaatttta 
      cgtaccctt acgggacca tatcttcag ttttcgacg 
      gccggttca gacacacca gtctcaaag acgggaaag 
      ccacgtttg ccaatttaa aaaaactcg acggaaaag 
      cagtttttc gctacaaag ttgttaatg caacggaga 
      gtctagact tattcgtga tcccatgtg ggggtgcga 
      ttgatatcg aagtaacat taaaaaacg caacggtgg 
      taccaccga aacgagtca aaagtttcg gcagaatca 
      ctataaaaa atccagtac agttcgccc ggtactaca 
      tcaagattt atgccctga accgcgaag aattgaaga 
      agacatcgg gagttgaaa tttatgcaa ccacacggt 
      ttccgacca aaattccct taccaaaca tgccccaaa 
      ccctcaaag tgacagaat acaagggtt tgaccactt 
      gtgatagtg tgacagtag cttggatgg cagcgccag 
      ggcagaaaa gtgcccatt actgccttt cggcaaccg 
      aaggaaaaa tccgggaga tggaagact acagaatgc 
      ccaaccaat gcgggcttg aaagtcgct tgaaaaaat 
      cagtcgact ttgctgtga aattttgca cagatgaga 
      aaaattaca aagaacctg tggtttaca aaacaataa 
      atcagctaa agcccggtc gcaattaca taatccaag 
      taactgtca aaaagccaa aagggaaaa ccttgtcgt 
      atgatcttc tccaagtgc atacgaaac cgtcctgca 
      gaaggaagc aagagtgac ttttagttt ggttttttc 
      attgattcc tgaatgagg ctagatgtt tatttattt 
      ttattattt ttactcagt tgtaagttt tatattttc 
      cagaataaa tatttttgt aaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY