Status
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Gene information
General information
TC005640 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10409397..10414088
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005640
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03994

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005640 RA
      MVARLLSTL LVLHSISVL LPSSCCRKK EPKLPVLQL 
HTNDSLPAD LQVTVGLPA GRNKSYSYT SPSAHGALS 
VTLTACTSP IFWSFGPDN TTMLHTHSM DTYHVPSPS 
QGVYTLTLL APESATLAH IYISTERGG PQALQSAKF 
SKVRLLKRQ KGKRLTVKW NPSLVDPQG TDYCLVVGT 
KPPVGSLCA AQQDPALAV ACVGRKTHH TITGLKHSQ 
KYYFSVFAI NNQSNFTYP YGTISSVFD GRFRHTSLK 
DGKATFANL KKLDGKAVF RYKVVNATE SLDLFVIPC 
GGAIDIEVT LKNATVVPP KRVKSFGRI TIKNPVQFA 
RYYIKIYAL NREELKKTS GVEIYATTR FPTKIPLPN 
MPQTLKVTE YKGLTTCDS VTVAWMAAP GQKSAHYCL 
SATEGKIRE MEDYRMPNQ CGLESRLKK SVDFAVKFC 
TDEKNYKEP VVYKTINQL KPGRNYIIQ VTVKKPKGK 
TLSYDLLQV HTKPSCRRK QE
    CDS sequence
    • >TC005640 RA
      atggtggcc cgtttgctg agcaccctg ttggtcctg 
cactccatt tcggtgctg ctccccagc agctgctgc 
cgcaagaag gagcccaaa ctgcccgtc ctccagctc 
cacaccaac gactccctg cccgccgac ctccaggtc 
accgtcggg ctgcccgcc ggccgcaac aaaagttac 
tcgtacaca tcgcccagc gcccacggc gcgctgtcc 
gtcaccctc acggcgtgc acgtcgccc attttttgg 
tccttcggg ccggataac accaccatg ctgcacacc 
cactccatg gacacctac catgtgccg tctcccagc 
cagggcgtg tacacgctc accctgctg gccccagaa 
tccgccacc ctggcccac atctacata agcacggag 
cgtggcgga ccccaagcg ctccagagc gccaaattc 
tccaaagtc cgtctgctc aagcgccag aaagggaag 
cggctaact gtgaagtgg aatccgagc ctggtggac 
ccgcaaggt accgactat tgtctggtg gtgggtacc 
aagccgccc gttgggtcg ctgtgtgcc gcacaacag 
gacccagca ctggctgtg gcttgtgtt ggccgcaag 
acccatcac accatcact ggtttaaag cacagccaa 
aagtactac tttagcgtc ttcgcgatc aacaaccaa 
tcgaatttt acgtaccct tacgggacc atatcttca 
gttttcgac ggccggttc agacacacc agtctcaaa 
gacgggaaa gccacgttt gccaattta aaaaaactc 
gacggaaaa gcagttttt cgctacaaa gttgttaat 
gcaacggag agtctagac ttattcgtg atcccatgt 
gggggtgcg attgatatc gaagtaaca ttaaaaaac 
gcaacggtg gtaccaccg aaacgagtc aaaagtttc 
ggcagaatc actataaaa aatccagta cagttcgcc 
cggtactac atcaagatt tatgccctg aaccgcgaa 
gaattgaag aagacatcg ggagttgaa atttatgca 
accacacgg tttccgacc aaaattccc ttaccaaac 
atgccccaa accctcaaa gtgacagaa tacaagggt 
ttgaccact tgtgatagt gtgacagta gcttggatg 
gcagcgcca gggcagaaa agtgcccat tactgcctt 
tcggcaacc gaaggaaaa atccgggag atggaagac 
tacagaatg cccaaccaa tgcgggctt gaaagtcgc 
ttgaaaaaa tcagtcgac tttgctgtg aaattttgc 
acagatgag aaaaattac aaagaacct gtggtttac 
aaaacaata aatcagcta aagcccggt cgcaattac 
ataatccaa gtaactgtc aaaaagcca aaagggaaa 
accttgtcg tatgatctt ctccaagtg catacgaaa 
ccgtcctgc agaaggaag caagagtga
    mRNA sequence
    • >TC005640 RA
      caaggagtc cgctgaaag aaattacag gcagtgagc 
tttcaggcc tgaagctga aaagtgaca attgtgtgg 
tgctttttc gggacgtta aggtcaaga tggtggccc 
gtttgctga gcaccctgt tggtcctgc actccattt 
cggtgctgc tccccagca gctgctgcc gcaagaagg 
agcccaaac tgcccgtcc tccagctcc acaccaacg 
actccctgc ccgccgacc tccaggtca ccgtcgggc 
tgcccgccg gccgcaaca aaagttact cgtacacat 
cgcccagcg cccacggcg cgctgtccg tcaccctca 
cggcgtgca cgtcgccca ttttttggt ccttcgggc 
cggataaca ccaccatgc tgcacaccc actccatgg 
acacctacc atgtgccgt ctcccagcc agggcgtgt 
acacgctca ccctgctgg ccccagaat ccgccaccc 
tggcccaca tctacataa gcacggagc gtggcggac 
cccaagcgc tccagagcg ccaaattct ccaaagtcc 
gtctgctca agcgccaga aagggaagc ggctaactg 
tgaagtgga atccgagcc tggtggacc cgcaaggta 
ccgactatt gtctggtgg tgggtacca agccgcccg 
ttgggtcgc tgtgtgccg cacaacagg acccagcac 
tggctgtgg cttgtgttg gccgcaaga cccatcaca 
ccatcactg gtttaaagc acagccaaa agtactact 
ttagcgtct tcgcgatca acaaccaat cgaatttta 
cgtaccctt acgggacca tatcttcag ttttcgacg 
gccggttca gacacacca gtctcaaag acgggaaag 
ccacgtttg ccaatttaa aaaaactcg acggaaaag 
cagtttttc gctacaaag ttgttaatg caacggaga 
gtctagact tattcgtga tcccatgtg ggggtgcga 
ttgatatcg aagtaacat taaaaaacg caacggtgg 
taccaccga aacgagtca aaagtttcg gcagaatca 
ctataaaaa atccagtac agttcgccc ggtactaca 
tcaagattt atgccctga accgcgaag aattgaaga 
agacatcgg gagttgaaa tttatgcaa ccacacggt 
ttccgacca aaattccct taccaaaca tgccccaaa 
ccctcaaag tgacagaat acaagggtt tgaccactt 
gtgatagtg tgacagtag cttggatgg cagcgccag 
ggcagaaaa gtgcccatt actgccttt cggcaaccg 
aaggaaaaa tccgggaga tggaagact acagaatgc 
ccaaccaat gcgggcttg aaagtcgct tgaaaaaat 
cagtcgact ttgctgtga aattttgca cagatgaga 
aaaattaca aagaacctg tggtttaca aaacaataa 
atcagctaa agcccggtc gcaattaca taatccaag 
taactgtca aaaagccaa aagggaaaa ccttgtcgt 
atgatcttc tccaagtgc atacgaaac cgtcctgca 
gaaggaagc aagagtgac ttttagttt ggttttttc 
attgattcc tgaatgagg ctagatgtt tatttattt 
ttattattt ttactcagt tgtaagttt tatattttc 
cagaataaa tatttttgt aaagt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY