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Gene information
General information
TC005624 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3451875..3455468
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005624
    iBeetle screen iB_03991
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005624 RA
      MVQARTADL VLTARDAFN KGFTKNMRY RVKNLKNFI 
      KFLEENETA IVEAMSDDL RKHREEARL EVFVAINHL 
      KYLIDNVRT WSEPDLAAK RWTDLLDGL YIYHDPLGV 
      VLVMGAWNV PLLTLVPVT GAMAAGNCV VIKPSHTCP 
      SFGKTLARL LPKYIDPIC YPVFLGGVE ETNELLEQR 
      FDYIYYTGG ATVGKIVHR AANKYLTPI TLELGGKNP 
      VFVDASADL ELTATRVMW GKCFNSGQA CIAPDYVLC 
      TKQVEKEFI QHARSALTK FFNNDPRCC PIVNDFHFR 
      RLQKLLRGL NVVIGGRVD PLDNYIEPT VVTGVTPAD 
      PIMQEEIFG PILPIITVS DFKDAVNFI NKGEKPLAL 
      YVFTKNAVV RDYIIDNTS SGGVTVNDT LMHLIVESL 
      PFGGVGLSG MGCYKGKDS FDTFVHKKS VLVKNFSRI 
      IEKVNEMRY PPYSSRKTD ILAFLLKYR RGLSWIWVF 
      YFSVFLIGF LVGFLFFNY FALVHYMK
    CDS sequence
    • >TC005624 RA
      atggtgcag gcccgaacg gccgacctg gtgctaaca 
      gcgcgcgat gccttcaac aaaggcttc accaagaac 
      atgcgctac cgcgtcaaa aacctcaag aacttcatc 
      aagttcctg gaggagaac gagaccgcg atcgtggag 
      gcaatgtcc gacgacctc cgcaagcac cgggaggag 
      gcccgcctt gaggtcttc gtggccata aaccacctc 
      aagtacctc atcgacaac gtgcgcacg tggtcggag 
      cccgacctg gcggccaag cgctggacc gacctcctg 
      gacggcctg tacatctac cacgacccc ttgggggtg 
      gtcctggtc atgggggcg tggaacgtc ccgctgttg 
      accctcgtt ccggtcaca ggcgccatg gccgccggc 
      aactgcgtg gtgatcaag ccgtcgcac acttgcccc 
      agctttggc aaaacgctg gcccggctt ttgcccaaa 
      tacatcgac cccatttgc taccccgtc ttcctgggc 
      ggggtcgag gagacgaac gagttgctg gagcagcga 
      tttgactat atttactac acagggggt gcgaccgtg 
      ggcaaaatc gtgcacagg gcggccaat aaatacttg 
      acccccata actttggaa ctagggggg aagaacccg 
      gtttttgtc gatgcgagt gccgatttg gaactgaca 
      gcgactcgg gtcatgtgg ggcaagtgt ttcaactcg 
      ggccaggcg tgcatcgcc cctgattac gtcctgtgt 
      acgaaacag gtcgagaag gaatttatc caacacgcc 
      cgaagtgct ttgaccaaa tttttcaac aatgaccct 
      aggtgttgc cccatcgtc aatgatttt cacttcaga 
      aggcttcag aaattgctc agggggctc aatgtggtg 
      atcgggggg cgcgtggac cccctggac aattacata 
      gagccgacg gttgtcaca ggggtaact ccagccgac 
      cccatcatg caagaggaa attttcgga ccaatcttg 
      ccaattatc acagttagt gattttaag gacgctgtg 
      aattttatc aacaagggc gaaaaaccc ctagctttg 
      tatgttttt acgaaaaat gccgtggtt agggattat 
      atcatcgat aatacttct tcgggtggt gttacagtc 
      aatgacacg ctcatgcat ttgattgtt gagagtttg 
      ccgtttgga ggagtgggt ttgagtgga atggggtgt 
      tataaagga aaggatagt ttcgatacg tttgttcat 
      aagaaaagt gtgttggtg aaaaatttt agcaggatt 
      atcgagaag gtgaatgaa atgaggtat ccgccgtat 
      tccagtcgg aaaactgat attttggcg tttttgctg 
      aagtacagg agagggctg tcctggatc tgggtgttt 
      tatttctcg gtttttttg atcgggttt cttgtaggt 
      tttctattt tttaattat tttgcccta gttcactat 
      atgaaataa
    mRNA sequence
    • >TC005624 RA
      ctaaatggt gtgggagag aactgctta cttgtaagg 
      tttttgaaa aacaaggac aaacagtat tgaagaacg 
      ttaaatagt ggaagaact tgtaaaatc aatatttta 
      ttacaacaa atactaagt taaaatcta ctttacata 
      acttagttt tttatatta tcggaaata tgatgctaa 
      ggagatgga actggaata attattaga attaccgac 
      ttcgactaa aataataaa aaatagaaa gacgcaggt 
      tttaaaaga aaataaaca gtgatcaat aataaaatt 
      gcgctgtaa taatgttat caaaataaa acgtagggc 
      gtcctaggt gaataaaaa ataaaaaaa caagcattt 
      ttaccctaa actaaatac ttggtactc gtattttat 
      gaaaaaaac tgaagctag taactatat ttaattaac 
      aacataaat aatcattct aattcgcct cagaagtaa 
      taataagag taacaaacg taaccggtt ttcaaaaat 
      attttttcg aaaatttta tgttgtcat attttttat 
      ttttattct tgacactgt caaaatttc ctttgtagg 
      gtagttttg actgtttgg catttctca atatgaaag 
      gtcagatta tttttaaaa tttaaaaaa aaagattta 
      agccttgtt gagtctaag tcgagaaaa aaaaagttg 
      tattcaact cgtttttgt gtaaatcgg ttcatttat 
      ttcgcctcg tggatgata aaccactcg tttatataa 
      taaaacgtc cgatttaca cccaaactc gttaaataa 
      ataaccact tttatagta acccacatc catggaaaa 
      ttgaaaaaa aatctcagg gtgaagata tgttttgtc 
      aattttatt ccagtttcc taagtgcgg tattttcaa 
      atgagttac agttttgca cataatttc gagtaaaat 
      atgcacaca caccgattt attatccat atttagatg 
      aataactgt tttgccgaa tacacgcag ctcataatg 
      gtcgcttta ttttccaac aaatagcca catgttacc 
      aaatgtcac gtactgtat gaattaaat aagccctag 
      cttgccact gaattataa aaactgaaa cgtttagaa 
      ttgagaaat atgaaataa gttattttt taacacata 
      atagttaca taacggtgc aatttgtaa ccaggtttg 
      tatcactat aataaatga tcaacacgt aggaggctt 
      actttcttt tattccaac caacctgaa aaaggaaga 
      aatatgtgg acatgctaa gcgcgtaac acttgcagt 
      cgcccaaaa gcgaattcg gaattttcc caaattcaa 
      aaccagtcc cttatctta atttcccgt gtagcagac 
      tttgaaact tttcagtca tgtcaatgt cacctcaat 
      tgcgcctga attgaatac acttgtggt gagtaaata 
      gcgtgattt tcatttgat gagtaaatt tacttcgag 
      caattacct ttgaataag tcatcacgc acctaatgc 
      cagtcaaca gaataaaga gttatgacg cttgagcga 
      ctcctccaa gtgtttcaa ttattagat aaccgatac 
      cgcgccaac tgattgaaa ttcagcgga gcttcgccc 
      caaagtggt gtcacaacg ccggccttc ggcaaaaat 
      acttaaacc cccttgcct ccaaaccaa cctcagtcc 
      ggcccagct ctataatgg tgcaggccc gaacggccg 
      acctggtgc taacagcgc gcgatgcct tcaacaaag 
      gcttcacca agaacatgc gctaccgcg tcaaaaacc 
      tcaagaact tcatcaagt tcctggagg agaacgaga 
      ccgcgatcg tggaggcaa tgtccgacg acctccgca 
      agcaccggg aggaggccc gccttgagg tcttcgtgg 
      ccataaacc acctcaagt acctcatcg acaacgtgc 
      gcacgtggt cggagcccg acctggcgg ccaagcgct 
      ggaccgacc tcctggacg gcctgtaca tctaccacg 
      accccttgg gggtggtcc tggtcatgg gggcgtgga 
      acgtcccgc tgttgaccc tcgttccgg tcacaggcg 
      ccatggccg ccggcaact gcgtggtga tcaagccgt 
      cgcacactt gccccagct ttggcaaaa cgctggccc 
      ggcttttgc ccaaataca tcgacccca tttgctacc 
      ccgtcttcc tgggcgggg tcgaggaga cgaacgagt 
      tgctggagc agcgatttg actatattt actacacag 
      ggggtgcga ccgtgggca aaatcgtgc acagggcgg 
      ccaataaat acttgaccc ccataactt tggaactag 
      gggggaaga acccggttt ttgtcgatg cgagtgccg 
      atttggaac tgacagcga ctcgggtca tgtggggca 
      agtgtttca actcgggcc aggcgtgca tcgcccctg 
      attacgtcc tgtgtacga aacaggtcg agaaggaat 
      ttatccaac acgcccgaa gtgctttga ccaaatttt 
      tcaacaatg accctaggt gttgcccca tcgtcaatg 
      attttcact tcagaaggc ttcagaaat tgctcaggg 
      ggctcaatg tggtgatcg gggggcgcg tggaccccc 
      tggacaatt acatagagc cgacggttg tcacagggg 
      taactccag ccgacccca tcatgcaag aggaaattt 
      tcggaccaa tcttgccaa ttatcacag ttagtgatt 
      ttaaggacg ctgtgaatt ttatcaaca agggcgaaa 
      aacccctag ctttgtatg tttttacga aaaatgccg 
      tggttaggg attatatca tcgataata cttcttcgg 
      gtggtgtta cagtcaatg acacgctca tgcatttga 
      ttgttgaga gtttgccgt ttggaggag tgggtttga 
      gtggaatgg ggtgttata aaggaaagg atagtttcg 
      atacgtttg ttcataaga aaagtgtgt tggtgaaaa 
      attttagca ggattatcg agaaggtga atgaaatga 
      ggtatccgc cgtattcca gtcggaaaa ctgatattt 
      tggcgtttt tgctgaagt acaggagag ggctgtcct 
      ggatctggg tgttttatt tctcggttt ttttgatcg 
      ggtttcttg taggttttc tatttttta attattttg 
      ccctagttc actatatga aataatact tactgtaga 
      aaagtatat tatctaaaa taaagcgat gttttttgt