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Gene information
General information
TC005623 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10518761..10543018
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005623
    iBeetle screen iB_03990

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005623 RA
      MENQSWTRK LLSTWRKDQ QWYADKLAI LRSVRSSEP 
      PPGDIQSPP TGETFKEYV VSEQTPGEL QRHEILYSS 
      VAVGVVLLV FISAAVLFF LRRRKTKNA DEEHAASSI 
      LVYPSQQGT APQFLTKEI HFSLPPLRS TSLGDLTEP 
      DDSDVDTES LLLPSVSNQ RSNSFSCYG LGVIEPALY 
      KSTLDLDEI QWPEGHIGR IWFSLRYEP STEKLLVSL 
      LKAKNLPSR TVGTVNSCD PFVRLHLMP DERRYLQSK 
      QKKKTCNPY FDETLVFQV SQKDMSDHI LKLTVIDGS 
      RTKRRSEIG HVTFPLKDL EIGDGTEQQ LFKLDLEKE 
      PQEIKSNLG ELLVSLLYN ENLSRLTAT VIEARGLKS 
      ADTRLKWTP DSLRFSKVR EMTRVESEL ILPAKQPLN 
      GFYNPVVVD IDEDTKQNE KPLQEVLNT ARTAFSCGK 
      SKPINFRKK QLKALEKFL IECEDEICE AVHKDLGKH 
      RQEASMGEI DLVKRDLRH TLFELSDWA KPVAPDRSI 
      LNLLDGVYI YHDPYGVVL IMSAWNYPF MLLLGPLIG 
      AIAGGNCVI LKPSELAVE SAKLIAKFL PRYLDKDCY 
      QVVLGGIED TTELLKHKF DYIFFTGSS SVGKIIHQA 
      AARHLTPTT LELGGKSPI YLDESADIL TATRRIIWG 
      RTMNAGQTC ISPDYLLCS KEVEEKFLA AARTVLEEF 
      YGDGDVKLS PYLSKIVLD RHYNRLMNF IKGSNVAIG 
      GSGDPRTRI IAPTVLIDV KPDDPVMLE EIFGPILPI 
      VTVNSPLEA INFINERDK PLALYVFTN RKDVKELFL 
      NNTSSGAVT VNDVIMHIW ADNLPFGGV GASGMGAYH 
      GKFGFDTFT HKKGVLERG LGKIAELTQ FARYPPYTK 
      SKGQFLAIA TKKRRGISV SWIKTALVF GLGVAFSYG 
      LQYICRDAD D
    CDS sequence
    • >TC005623 RA
      atggaaaat caatcgtgg acccgtaaa ctgctgagc 
      acttggcgc aaggatcaa caatggtac gctgacaaa 
      ttggcgatc ctgcggagc gtgcggtcg tcggagcct 
      ccgccggga gacatccaa tcgccacca acaggagag 
      accttcaaa gagtacgtg gtctcggaa caaactccc 
      ggagaattg caacggcac gaaatcctg tattcgtcg 
      gtggctgtg ggcgtcgtc ctgcttgtc tttatttcg 
      gcggctgtg ttgtttttc ctccgtcgt cgcaaaact 
      aaaaacgct gacgaggaa cacgccgct tccagcatc 
      cttgtttac ccatctcag caaggaacc gcgcctcag 
      tttttgacc aaagagatc catttcagt cttccacct 
      ttaaggagc acctcacta ggcgacctc actgaaccg 
      gatgattcc gatgtcgac accgagagt ttactgctt 
      ccttcggtc tcgaatcag agatcgaat tctttcagt 
      tgttacggc ttaggggtg atagaaccc gccctgtat 
      aaaagcaca ctggatttg gacgaaatc cagtggccg 
      gaagggcac attggccgg atctggttc tcgctaagg 
      tacgaacct tcgacggaa aaattgctg gtctccttg 
      ctcaaggcc aagaatttg ccctcgagg actgtgggc 
      acggtcaac agctgcgac ccctttgtt cgcttgcac 
      ttaatgccc gacgaaaga cgttacttg caatccaaa 
      cagaagaaa aagacttgc aacccgtac tttgacgaa 
      actctggtc ttccaagtc tcgcagaaa gatatgtct 
      gatcacatc ctgaagctg acagtaatc gacggcagc 
      aggactaag cgaagatcc gaaatcgga cacgtgacg 
      tttccgctc aaggatttg gaaattggg gacgggacc 
      gaacagcag cttttcaag ttggacttg gaaaaggag 
      ccgcaagag atcaagtcg aatctgggg gagttgctt 
      gtgtcgctg ctctacaac gaaaatttg agtcggctt 
      acggcgact gtgatagag gccaggggg ttgaagtcg 
      gcggacact cgtttgaaa tggacgccc gactcgctg 
      agattcagc aaagttcgc gaaatgacc cgagtggaa 
      agtgaactt atcttgcct gcgaagcag ccactcaac 
      gggttctac aacccggtg gtggtcgac atcgacgaa 
      gacaccaaa cagaacgag aagcccctc caggaggtg 
      ctcaacacg gccaggacg gccttcagc tgcggcaaa 
      tccaagccg atcaatttc cggaaaaag cagctcaag 
      gcgcttgaa aagttcctc atcgagtgt gaagacgaa 
      atttgcgaa gccgtccac aaagacctg gggaagcac 
      cgccaggag gcctccatg ggggagatc gatttggtc 
      aagcgcgac ctcagacac accctcttc gagctgtcg 
      gattgggca aaacccgtg gctccagac cggagcatc 
      ctgaacctc ctcgacgga gtctacatc taccacgat 
      ccctacggc gtggtcctc attatgagc gcgtggaac 
      taccccttc atgctcctc ctggggccc ttgataggt 
      gctatcgcc ggcggaaac tgcgttatc ctcaaaccg 
      tcggaactg gccgtcgaa tcggcgaaa ctcatcgcc 
      aaattcctg ccccggtac ctggacaag gactgctac 
      caggtggtg ctgggcggg attgaggac accacagag 
      ctgctcaaa cacaagttc gactatatc ttcttcacc 
      gggtcgtca agcgtcggg aaaatcatc caccaagcg 
      gccgccaga cacctgacc cccaccacg ctggaactg 
      gggggcaaa agccccatt tacttggac gaatccgcg 
      gatattttg actgcgact cgccggatc atttggggg 
      aggaccatg aacgcgggc caaacgtgc atctccccc 
      gattacctc ctctgctcg aaggaggtc gaggagaag 
      ttcctggca gctgcgcgt acagttctt gaggagttt 
      tacggtgat ggggacgtc aaactcagc ccttattta 
      agcaaaatc gtcctggac cgccactac aaccgcctc 
      atgaacttc atcaagggc tcgaacgtg gcaattggg 
      ggaagtggc gaccccagg accaggatc atcgccccc 
      acggtcctc atcgacgtc aagcccgac gacccggtg 
      atgttggag gaaattttc gggccgatt ctccccatt 
      gtcaccgtg aattcgccc ttggaggcc atcaatttc 
      atcaacgag cgcgacaag cccttggcc ctttacgtt 
      ttcaccaat cgcaaggac gtgaaggag ttgttcttg 
      aataacacc tcaagtggg gcggtgact gtcaacgac 
      gtgatcatg cacatttgg gccgataat ttgcccttc 
      ggaggggtg ggcgcgagc gggatgggg gcctaccat 
      ggcaagttc gggttcgac actttcacg cataaaaag 
      ggcgttttg gagcggggg ttggggaaa atagcagag 
      ttgacgcag tttgcgagg tatccgccg tacaccaag 
      tcaaagggc cagttcctg gcaattgcc acgaagaag 
      cggcgcggg atcagcgtc agttggatc aagacggcg 
      ttggttttt gggttaggg gtggcgttt tcgtacggc 
      ttgcaatac atttgtcgg gacgccgat gattaa
    mRNA sequence
    • >TC005623 RA
      cgcgtcgta tttcgtagt ccgctcctg ttgcaggct 
      aaatatgtg aatccccga ccgcccata atggaaaat 
      caatcgtgg acccgtaaa ctgctgagc acttggcgc 
      aaggatcaa caatggtac gctgacaaa ttggcgatc 
      ctgcggagc gtgcggtcg tcggagcct ccgccggga 
      gacatccaa tcgccacca acaggagag accttcaaa 
      gagtacgtg gtctcggaa caaactccc ggagaattg 
      caacggcac gaaatcctg tattcgtcg gtggctgtg 
      ggcgtcgtc ctgcttgtc tttatttcg gcggctgtg 
      ttgtttttc ctccgtcgt cgcaaaact aaaaacgct 
      gacgaggaa cacgccgct tccagcatc cttgtttac 
      ccatctcag caaggaacc gcgcctcag tttttgacc 
      aaagagatc catttcagt cttccacct ttaaggagc 
      acctcacta ggcgacctc actgaaccg gatgattcc 
      gatgtcgac accgagagt ttactgctt ccttcggtc 
      tcgaatcag agatcgaat tctttcagt tgttacggc 
      ttaggggtg atagaaccc gccctgtat aaaagcaca 
      ctggatttg gacgaaatc cagtggccg gaagggcac 
      attggccgg atctggttc tcgctaagg tacgaacct 
      tcgacggaa aaattgctg gtctccttg ctcaaggcc 
      aagaatttg ccctcgagg actgtgggc acggtcaac 
      agctgcgac ccctttgtt cgcttgcac ttaatgccc 
      gacgaaaga cgttacttg caatccaaa cagaagaaa 
      aagacttgc aacccgtac tttgacgaa actctggtc 
      ttccaagtc tcgcagaaa gatatgtct gatcacatc 
      ctgaagctg acagtaatc gacggcagc aggactaag 
      cgaagatcc gaaatcgga cacgtgacg tttccgctc 
      aaggatttg gaaattggg gacgggacc gaacagcag 
      cttttcaag ttggacttg gaaaaggag ccgcaagag 
      atcaagtcg aatctgggg gagttgctt gtgtcgctg 
      ctctacaac gaaaatttg agtcggctt acggcgact 
      gtgatagag gccaggggg ttgaagtcg gcggacact 
      cgtttgaaa tggacgccc gactcgctg agattcagc 
      aaagttcgc gaaatgacc cgagtggaa agtgaactt 
      atcttgcct gcgaagcag ccactcaac gggttctac 
      aacccggtg gtggtcgac atcgacgaa gacaccaaa 
      cagaacgag aagcccctc caggaggtg ctcaacacg 
      gccaggacg gccttcagc tgcggcaaa tccaagccg 
      atcaatttc cggaaaaag cagctcaag gcgcttgaa 
      aagttcctc atcgagtgt gaagacgaa atttgcgaa 
      gccgtccac aaagacctg gggaagcac cgccaggag 
      gcctccatg ggggagatc gatttggtc aagcgcgac 
      ctcagacac accctcttc gagctgtcg gattgggca 
      aaacccgtg gctccagac cggagcatc ctgaacctc 
      ctcgacgga gtctacatc taccacgat ccctacggc 
      gtggtcctc attatgagc gcgtggaac taccccttc 
      atgctcctc ctggggccc ttgataggt gctatcgcc 
      ggcggaaac tgcgttatc ctcaaaccg tcggaactg 
      gccgtcgaa tcggcgaaa ctcatcgcc aaattcctg 
      ccccggtac ctggacaag gactgctac caggtggtg 
      ctgggcggg attgaggac accacagag ctgctcaaa 
      cacaagttc gactatatc ttcttcacc gggtcgtca 
      agcgtcggg aaaatcatc caccaagcg gccgccaga 
      cacctgacc cccaccacg ctggaactg gggggcaaa 
      agccccatt tacttggac gaatccgcg gatattttg 
      actgcgact cgccggatc atttggggg aggaccatg 
      aacgcgggc caaacgtgc atctccccc gattacctc 
      ctctgctcg aaggaggtc gaggagaag ttcctggca 
      gctgcgcgt acagttctt gaggagttt tacggtgat 
      ggggacgtc aaactcagc ccttattta agcaaaatc 
      gtcctggac cgccactac aaccgcctc atgaacttc 
      atcaagggc tcgaacgtg gcaattggg ggaagtggc 
      gaccccagg accaggatc atcgccccc acggtcctc 
      atcgacgtc aagcccgac gacccggtg atgttggag 
      gaaattttc gggccgatt ctccccatt gtcaccgtg 
      aattcgccc ttggaggcc atcaatttc atcaacgag 
      cgcgacaag cccttggcc ctttacgtt ttcaccaat 
      cgcaaggac gtgaaggag ttgttcttg aataacacc 
      tcaagtggg gcggtgact gtcaacgac gtgatcatg 
      cacatttgg gccgataat ttgcccttc ggaggggtg 
      ggcgcgagc gggatgggg gcctaccat ggcaagttc 
      gggttcgac actttcacg cataaaaag ggcgttttg 
      gagcggggg ttggggaaa atagcagag ttgacgcag 
      tttgcgagg tatccgccg tacaccaag tcaaagggc 
      cagttcctg gcaattgcc acgaagaag cggcgcggg 
      atcagcgtc agttggatc aagacggcg ttggttttt 
      gggttaggg gtggcgttt tcgtacggc ttgcaatac 
      atttgtcgg gacgccgat gattaattt taagggcgg 
      gtttggaat tttctgtga tatgtactg tcgtataca 
      gggtgatga caaattgat ttttgagaa aaatgtaca 
      agttccttt atagattag tcgacgagt gactgaatc 
      aatatgcta tctatacag ggaaaaatt ttaattata 
      tattttgta acgatttat cgaccgttg gcatatttt 
      tttaatatg tgcagctgc cacgttgta aaatatgga 
      tagtttaaa gaggagtcg agagagctc ggtggcctt 
      ggtgaataa ttaatttga aatgtttga aacaaaacg 
      acatctttt ttaatagtt tgtatttat tttggcaaa 
      gtttattcg catttctca actcaaggc tgaaactgt 
      tatacctac cgagagtga atgttttat agtatttag 
      aacataaac agcacaagg taatcaatt agaaatgtc 
      tcaattatt taggtacaa aaataattg gtaaaatga 
      atgtaggaa atgtacaaa caat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY